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ABSTRACT POSTER UEU

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Nurul Fajriyah
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8 views1 page

ABSTRACT POSTER UEU

Uploaded by

Nurul Fajriyah
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Antioxidant Activity Test and Determination of Total Phenolic

and Flavonoid Content of 80% Ethanol Extract of Sungkai


Leaves (Peronema canescens Jack)

Nurul Fajriyah Haerunnisa, Muchammad Reza Ghozaly, Yonatan Eden

Pharmacy Study Program, Faculty of Health Sciences, Esa Unggul University, Jakarta,
Indonesia

[email protected] [email protected]

Abstract
Sungkai (Peronema canescens Jack) is a plant that has potential as a medicinal plant.
Sungkai belongs to the Lamiaceae family and is geographically distributed in several areas.
Sungkai leaves have bioactive compounds in the form of alkaloids, flavonoids, phenolics, and
saponins (Pindan et al., 2021) which are believed to have antioxidant activity. This study aims
to determine the total flavonoid content, total phenols and antioxidant activity of 80% ethanol
extract of Sungkai leaves (Peronema canescens Jack). Total flavonoid content testing was
carried out using the colometry method with AICI3 reagent. Total phenol testing was carried
out with Folin Ciocalteu reagent. Meanwhile, antioxidant activity testing uses the DPPH (2,2-
Diphenyl-1picryl Hidrazil) method with results expressed in Inhibition Concentration 50%
(IC50). The test results showed that the total flavonoids in the thick extract of sungkai leaves
were 40,08 mgQE/g, while the total phenol test in the thick extract of sungkai leaves was 67,41
mgGAE/g. Antioxidant activity testing showed that the IC50 value obtained was 54,37 μg/mL,
and the antioxidant value for vitamin C was 17,22 μg/mL. This shows that sungkai leaves have
strong antioxidant activity >50 μg/mL and are rich in flavonoid and phenolic compounds.

Key words: Sungkai leaves (Peronema canescens Jack), Total Flavonoids, Total Phenols,
Antioxidant Activity, IC50.

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