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Biochemistry PDF

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Misana Thomas
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10 views

Biochemistry PDF

Uploaded by

Misana Thomas
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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1.

Each deoxyribonucleoside is composed of

a. Purine and pyrimidine

b. Thyamine and purines

c. Nitrogen bases and a sugar deoxyribose

d. Deoxyribose sugar and purine

2. The structural components of the ribosome is

a. tRNA

b. mRNA

c. SRNA

d. TRNA

3. Genetic information in biological systems is often depicted in a scheme known as

a. 'gene dogma'

b. 'genetic dogma"

c. 'central dogma'

d. 'dogma'

4. The information stored in the DNA of the organism is

a. Genotype

b. Phenotype

c. Both A and B

d. None of the above

Which one of the following is NOT a method of protein extraction?


a.

Sonication,

b. Homogenization by high pressure

Photos

c. Elution

d. Repeated freezing and thawing

6. In protein purification the specific assay can be bared upon the following EXCE

a. Enzymatic activity

b. Immunological activity

c. Chemical activity

d. Physical characteristics

7. In chromatography protein separation is based on

a. Size, shape or ionic interaction

b. Size, affinity or ionic interaction

c. Size, shape or affinity

d. Size, affinity or structure

8. The following are fat soluble vitamins, which one is NOT.

a. Vitamin A

b. Vitamin K

c. Vitamin B

d. Vitamin E
9. Amphoteric substances are

a. Substances that can react with an acid and a base

b. Substance that can act as an acid or a base

c. Substances that cannot react with an acid and a base

d. All the above

10. Proteins structure differ from each other by

a. -NHz (amino group)

b. -COOH

c. -R chain

d. -a Carbon

11. A method of writing structural formula of carbohydrates to represent m structure with a


simple 3D perspective is known as

a. Haworth projection

b. Structural formula

c. Empirical formula

d. Simple formula

12. Glucose is a monosaccharide and is a

a. hexose

b. pentose

c. furanose

d. sucrose
13. A 45-year-oid female presents to her primary care doctor with fatigue and tingling/numbness
her extremities (bilateral). Examination reveals a beefy red and fissured tongue. Further
evaluation reveals a low Glutathione reductase activity in the red blood cells. Which of the
following vitamin deficiencies could have caused her symptoms?

a. Vitamin C deficiency

b. Niacin deficiency

c. Vitamin B2 deficiency

d. B6-P deficiency.

14. A 23-year-old male, golden gloves boxing contender presents with metabolic disorder. He
describes his training regimen that involves consuming a dozen raw eggs a day for a protein Raw
eggs contain a protein called Avidin; with an extremely high affinity for a cofactor, req by
Propionyl CoA carboxylase, Acetyl CoA carboxylase and pyruvate carboxylase. The patie
deficient in which of the following co factor?

a. Cobalamine

b. Biotin

c. Folic acid

d. Pyridoxal phosphate

15. Compounds having the same structural formula but they differ in spatial configuration

a. Diastreoisomers

b. Isomers

c. Anomers

d. Stereoisomers

16. Which technique uses the attraction of the protein for a particular chemical group dur protein
purification?

a. Affinity chromatography
b. Gel-filtration chromatography

HPLC

d.

SDS PAGE

17. Which of the following described the quaternary structure of a protein? a.

Helix or sheet stabilized by hydrogen bonds

b. Formation of all four kinds of noncovalent bonds

C.

Requires disulfide bonds

d. None of the above are correct

18. The nucleic acid base found in mRNA but NOT in DNA is

a. Adenine

b. Cytosine

c. Guanine

d. Uracil

19. The protein present in hair is

a. Keratin

b. Elastin

c. Tropocollagen

d. Myosin
20. The site of protein synthesis is a.

Ribosome

b. Nucleus

6. Endoplasmic reticulum

d. Chromosome

SECTION B (30 MARKS) answer all questions

1. Describe the following terms

i) Epimer

11) Stereoisomer

2. Draw an illustration of the structure of DNA double helix

3. Differentiate between amylose and amylopectin

4. Draw a structure of a phosphophytidyl ethanolamine

5. State the functions of following

i) Topoisomerase ii Primase

ill) Helicuse

SECTION C (20 MARKS) answer any one question

1. Explain the properties of genetic codes

2. Discuss the functions of biomembranes

3. Discuss different types of chromatographic techniques

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