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Genetics Lab Midterm Exam (2)

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17 views

Genetics Lab Midterm Exam (2)

Uploaded by

Jett
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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University of Balamand

FAS
Department of Biology

Genetics Laboratory BIOL 284


Fall 2020

Online Examination

Name : __________________

ID : __________________

Grading: The point value is indicated with each question

Guidelines:

Please, carefully read what follows:

The time allocated for this exam is 60 minutes


Write your NAME and ID on THE COVER PAGE
Fill your answers by preferably using a different font color

Submit the completed questionnaire at the end of the exam by sending it by email
to: [email protected]

Do not delete any page from this questionnaire (Exam will be cancelled)
In case of suspicious cheating, the exam will be cancelled
You have a maximum of 2 minutes delay in order to send your online exams by
email; otherwise, the examination will be canceled
What is the use and composition of a running buffer? Sample buffer? (4 points)

Why is a semi log graph always crucial in accurately determining DNA size during agarose gel
electrophoresis? (6 points)

What are the two techniques that are widely used to perform DNA fingerprinting analysis?
Briefly discuss the concept behind those techniques stating the advantages and disadvantages
of each. (6 points)

You are studying a rare autosomal recessive human disease that is fully penetrant in adults. You
have identified a restriction fragment length polymorphism (RFLP) that is 10 map units from
the disease gene. Consider the following pedigree and Southern blot showing inheritance of the
RFLP. Individual D is too young to show the disease trait.
Using the above pedigree and ignoring the RFLP data, what is the probability that individual
D will be affected by the disease as an adult? (3 points)

Considering all the data, give the genotypes for the individuals F, C, and E. Define any
symbols used (4 points)

Considering all the data, what is the probability that individual D will be affected by the
disease as an adult? (3 points)

A common epistatic trait is albinism (lack of pigment). You are all familiar with white
laboratory rats. These are albino rats. Rats may also have agouti (grey) or black coats. These
are determined by the alleles G and g. The genes for albinism are a separate set of alleles.
These genes are either A or a.
Key: G = gray g = black A = color or pigment is present a = color or pigment is absent

What color would a rat who is GGaa be? How about if the rat was genotype ggAa? (2 points)

Try writing the genotypes for the following rats. (2 points)


Gray, homozygous for both traits:
Black, homozygous for both traits:
Gray, heterozygous for both traits:
Black, heterozygous for pigment:

• Two black rats have albino and black offspring. Please show all the genotypes of the
parents and progeny of this cross (with the appropriate ratios). (4 points)
Is it possible for these parents to produce any gray offspring? Why or why not? (2 points)

• A gray rat, heterozygous for both traits, is mated with a black rat heterozygous for
pigmentation. Please show all the phenotypes of the progeny of this cross (with the
appropriate ratios). (4 points)

The key portion of the autoradiograph from a single locus probe analysis of various DNA
samples in a rape investigation is shown in this figure.

Samples of DNA were loaded into the following lanes:


1. known blood sample of victim
2. known blood sample from defendant
3. DNA size markers
4. female fraction from vaginal swab of victim
5. male fraction from vaginal swab of victim

If you are the DNA analyst, what do you conclude by looking at the autoradiograph? (10
points)

The trait for smooth peas (W) is dominant over wrinkled peas (w) and yellow pea colour (G)
is dominant to green (g)
A dihybrid cross between two heterozygous pea plants is performed (WwGg × WwGg)
The following phenotypic frequencies are observed:

▪ 695 smooth yellow; 199 smooth green; 250 wrinkled yellow; 70 wrinkled green

Based on what you learned in class about statistical testing methods, do you think that the 2
genes are linked or not? (10 points)

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