Scribd
Upload
19 views2 pages

ABO lab report

A lab report that entails the abstract, introduction, requirements, procedure, outcomes, explanation and conclusion is conducting the ABO blood test.

Uploaded by

iloveodds1out
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
19 views2 pages

ABO lab report

A lab report that entails the abstract, introduction, requirements, procedure, outcomes, explanation and conclusion is conducting the ABO blood test.

Uploaded by

iloveodds1out
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
You are on page 1/ 2

Unit name: Biochemistry of biomolecules

Objective: Identify different types of blood groups using various anti sera
Practical no: 003
TITLE:ABO BLOOD GROUPING
Abstract
This experiment was carried out to determine the blood type of individuals using the ABO blood
group system. Blood samples were mixed with anti-sera to observe agglutination reactions.
Agglutination of red blood cells with anti-A serum indicated the presence of A antigens.
Agglutination with anti-B serum indicated B antigens. Agglutination with both A and B anti sera
indicated presence of AB antigens. No agglutination with both sera shows O blood group. The
Rh factor was tested using anti-Rh serum. Rh+ showed agglutination while Rh- had no
agglutination.
Introduction
Blood groups are determined by the presence or absence of antigens(A and B) on the surface of
red blood cells. The ABO system classifies blood into four main types: A, B, AB, and O. The Rh
factor, classifies blood as Rh+ or Rh-. Blood typing is useful in blood transfusion to ensure
compatibility. This experiment was done to determine the ABO and Rh blood types of students
by mixing their blood with corresponding anti-sera and observing agglutination reactions.
Reagents and materials
1. Applicator sticks
2. Blood samples from volunteers
3. Anti A serum
4. Anti B serum
5. Rhesus serum
6. Distilled water
7. Spotting tiles
8. Capillary tube
9. Lancet
10. Cotton wool
Procedure
1. Using cotton wool soaked in ethanol, clean the volunteers finger.
2. Using the index and thumb and index finger push blood to the tip of the finger.
3. Using the lancet, prick the finger and use the capillary tube to collect the blood.
4. Transfer the blood to the spotting tile and label A, B and Rh for each of the sera.
5. Add a drop of each sera to the respective blood samples and mix each with its own
applicator stick.
6. Gently rock the spotting tiles to allow for agglutination.
7. Record your observations after 2-5 minutes and make relevant conclusions to identify the
blood group.
Results and Conclusion
Volunteer Observation Conclusion
Lorena Iha Agglutination with anti A Volunteer is of A+ blood
serum group
Agglutination with Rh serum
No agglutination with Anti B
serum
Angela Wagithi No agglutination with anti A Volunteer is of O+ blood
serum group
Agglutination with Rh serum
No agglutination with Anti B
serum
Emmanuel Musyoka Agglutination with anti A Volunteer is of A+ blood
serum group
Agglutination with Rh serum
No agglutination with Anti B
serum

Discussion
The ABO and Rh blood grouping system is based on agglutination reaction. Agglutination is the
reaction between antigens present on red blood cells and antibodies present in serum resulting in
visible clumping. Agglutination occurs if an antigen is mixed with its corresponding antibody,
i.e. occurs when A antigen is mixed with anti-A or when B antigen is mixed with anti-B.
REFERENCE
Kenyatta university laboratory practical manual

You might also like