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4.Microscope.sp23

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1 views

4.Microscope.sp23

Uploaded by

pandastacy13
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Lab Talk 4

Base
Arm List of terms- Lab 4
Lamp
Total magnification (eyepiece X objective)
Condenser
Iris diaphragm Resolution
Mechanical stage
Mechanical stage knobs Orientation of objects on a slide (letter “e”)
Mechanical stage clips
Ocular (Eyepiece) Lens (10X) Effect of changing to higher power on:
Objective Lenses: • field of view (how much of the slide you
• Scanning lens (4X) can see) (slide of letter “e”)
• Low power lens (10X) • amount of light
• High Dry lens (40X) • depth of focus (slide of 3 colored threads)
• Oil immersion lens (100X)
Coarse focus (adjustment) • Stain (slide of cheek cells)
Fine focus (adjustment)
Factors that Assist in Viewing a Structure Using a
Brightfield Microscope

Magnification - enlarging the structure 40 – 1000 times


using different lenses

Resolution – separating two structures in space. Quality


of lenses prevents “blurry” images.

Contrast – stains are used to give different coloration to


different cellular structure
Brightfield Microscope
Steps to Magnify and Focus a Specimen

(1) Observe your slide and look for location of stain. That is probably
where the structure you are interested is located.
(2) Place the slide on the microscope, so that the stained area is centered
over the light coming from the light source.
(3) Make sure the oculars are the right distance for your eyes (this allows
binocular viewing of the specimen)
(4) Start with 4X (scanning) lens, so that you have the largest field of view
(amount of slide you can see through the eyepiece)
(5) Center the item you want to magnify within the field of view.
(6) Focus at 4X using coarse focus knob. Then, adjust with fine focus knob.
(7) Center area of interest. Switch to 10X. Use fine focus.
• Can magnify objects 40 – 1000 times.
• Magnifying lens
• Ocular lens (10 fold magnification)
• Objective lenses – 4 different magnifications
• Rotate into place

ocular X objective = total magnification


10 X 4 (scanning) = 40
10 X 10 (low power) = 100
10 X 40 (high dry) = 400
10 X 100 (oil immersion) = 1000
Distance between the objective and the
slide under the microscope.
Field of View – Viewing the Letter “E” (e)
4X 10X 40X

How is the letter “e” oriented? Right to left? Top to bottom?


As you magnify:
- does the “circle of light” change in size? increases / decreases / same
- does the object (letter “e”) change in size? increases /decreases/same
- does field of view change in size (how much can you see on the slide?)
increases / decreases / same
Depth of Focus – Viewing 3 threads
10X 40X

As you magnify:
- does the “circle of light” change in size? increases / decreases / same
- does the depth of focus change (how well you can see what is above
or below)? increases / decreases / Same
- does the amount of light change? increases/decreases/ Same
Stain – used to visualize structures
- most slides used in microscopy are stained with dyes that give
contrast. The nucleus becomes visible. Different cells and tissues
often absorb dyes differently, some staining shades of blue or red.
Cheek cell – slide of epithelium that lines
unstained stainedthe cheek

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