BIG IDEA 2

EDVO-Kit: AP05

Photosynthesis

See Page 3 for storage instructions.

EXPERIMENT OBJECTIVE:
In this experiment, students will learn how to measure the rate
of photosynthesis indirectly by using the floating leaf disk
method. They will also investigate several factors that
might affect the photosynthesis process.

EVT AP04.130109
 EX PERIMENT

AP05 Photosynthesis

Table of Contents
Page

Experiment Components 3
Experiment Requirements 3
Background Information 4

Experiment Procedures 6
Experiment Overview 6
Investigation I: Observation of environmental factors that affect
the rate of photosynthesis 7
Investigation II: Observation of plant respiration 10
Data Collection and Analysis 12
Study Questions 14

Instructor’s Guidelines
Notes to the Instructor 15
Pre-Lab Preparations 16
Experiment Results and Analysis 17
Study Questions and Answers 19

Material Safety Data Sheets 20

The Advanced Placement (AP) Program is a registered trademark of the College Entrance Examination Board. These
laboratory materials have been prepared by EDVOTEK, Inc. which bears sole responsibility for their contents.

All components are intended for educational research only. They are not to be used for diagnostic or drug pur-
poses, nor administered to or consumed by humans or animals.

THIS EXPERIMENT DOES NOT CONTAIN HUMAN DNA. None of the experiment components are derived
from human sources.

EDVOTEK and The Biotechnology Education Company are registered trademarks of EDVOTEK, Inc.

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 E XP E RIME N T

Photosynthesis
AP05

Experiment Components
Store the entire
experiment at room
• Sodium Bicarbonate (baking soda) temperature.
• Liquid soap
• Plastic syringes This experiment is
• Transfer pipets designed for 10 lab
• Plastic cups groups.

Requirements

• Leaves (i.e. spinach, ivy, pokeweed)

• Timer
• Light source (60 watt light bulb recommended)
• Hand-held hole punch
• Beakers

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 EX PERIMENT

AP05 Photosynthesis

Background Information

Photosynthesis is the process by which plant cells use light energy for the biosynthesis of
cellular components. Photosynthetic organisms form the basis of the food chain. These
life forms include higher plants, algae, dinoflagellates, euglenoids, diatoms and certain
bacteria. Photosynthesis consists of two biochemical phases. The general equation for the
first phase is:

H2O + NADP+ + Pi + ADP + Light → O2 + NADPH + H+ + ATP

The first phase is light dependent. NADP+ and NADPH are oxidized and reduced forms,
respectively, of nicotinamide adenine dinucleotide phosphate. The reduced form is an
essential cofactor in the biosynthesis of many types of molecules such as carbohydrates.
Chemical energy of ATP is required for many biochemical reactions and for maintenance
of cellular integrity and function. ATP is generated from ADP and inorganic phosphate
(Pi). The reaction also generates protons (H+) and molecular oxygen from water. The sec-
ond phase of photosynthesis can be generally written as:

CO2 + NADPH + H+ + ATP → glucose + NADP+ + ADP + Pi

The second phase is not light dependent. The reaction fixes atmospheric carbon dioxide
into organic linkage (glucose). Each phase consists of many separate chemical steps. First
phase steps are called light reactions and second phase steps are called dark reactions.

Light reactions in eukaryotic cells occur in organelles called chloroplasts. Chloroplasts

contain DNA and are self-replicating. These organelles consist of an outer membrane and
a folded inner membrane. Stacked, disk- like structures called thylakoids form part of the
inner membrane and it is here that light dependent photosynthetic systems are found.
The primary photosynthetic pigments are green chlorophylls. Chloroplasts contain chloro-
phyll ‘a’ and ‘b’, magnesium-porphyrin complexes, and are specifically bound to proteins
that reside on and within the inner membrane. Pure chlorophyll ‘a’ maximally absorbs
light at wavelengths of around 420 and 660 nm. Chlorophyll ‘b’ absorbs primarily at ap-
proximately 480 and 640 nm.

The absorption spectrum of chlorophylls can be shifted depending on with which type
of protein they are associated. Other pigments found in chloroplasts include ß-carotene
and xanthophylls. These pigments have an accessory light harvesting function and absorb
at wavelengths in between the maxima of the chlorophylls. They all capture light energy
and transfer it to the chlorophyll a at the reaction center. All these pigments are sensi-
tive to light and oxygen in the purified state and eventually breakdown. Extremely pure
preparations required for chemical and biological studies are stored under vacuum, in the
dark, at -20° C.

Photosynthetic System

There are two photosynthetic systems in chloroplasts, termed Photosystem i and ii. These
physically distinct systems contain different proteins and ratios of chlorophylls and acces-
sory pigments. Photosystem i is not responsible for oxygen evolution and is activated by
longer wavelengths of light. Photosystem ii is activated by shorter wavelengths of light
and is required for oxygen and ATP production. Both systems contribute high energy
electrons for the reduction of NADP+. Both photosystems are required for maximal pho-
tosynthetic activity. When light is absorbed by the chlorophyll-protein complex of pho-
tosystem I, chlorophyll becomes excited and enters a higher energy state. During return

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 E XP E RIME N T

Photosynthesis
AP05
Background Information

from high energy to ground state, an electron is boosted to a higher energy level and is
sequentially transferred, via several membrane associated protein transport molecules, to
the final electron acceptor NADP+. The NADP+ is reduced to NADPH through the action
of a reductase enzyme. Since this is not normally a spontaneous (energetically favorable)
reaction, input of light energy is required to convert NADP+ to NADPH.

Electron transport proteins containing iron and sulfur are called ferredoxins. Other trans-
porters are called cytochromes. A high energy electron generated by light absorption in
photosystem II is donated, via a specific sequence of transporters, to the electron defi-
cient photosystem I. Photosystem II then receives an electron through a series of transport
proteins from H20. Water is oxidized to molecular oxygen during this process. Water is the
electron donor in photosynthesis. Conversion of water, a very stable molecule, to oxy-
gen is energetically unfavorable and would not occur to any significant extent without
input of light energy at photosystem II. Production of ATP occurs along the sequence of
electron transfer steps. ATP is a high energy compound and requires energy for its syn-
thesis. This energy is siphoned from the high energy electrons through a complex series
of events involving membrane proteins and formation of pH gradients across chloroplast
membranes to drive ATP synthesis.

The dark reactions of photosynthesis occur simultaneously with the light reactions in
plant cells. The dark reactions are a set of seven enzyme catalyzed metabolic steps that
synthesize glucose. The key metabolic step in plant glucose synthesis is catalyzed by the
abundant enzyme ribulose diphosphate carboxylase. The majority of these reactions take
place outside the chloroplast in the cytoplasm. Most of the glucose is polymerized into
starch and cellulose. The reaction involves the fixation of carbon dioxide.

This experiment uses the floating disk leaf assay to explore the process of photosynthesis
in plants. Leaf disks generally float due to the many intercellular spaces used for ex-
change of gases. When the air spaces are infiltrated with solution, the overall density of
the leaf disk increases, causing the disks to sink. The infiltration solution includes a small
amount of Sodium bicarbonate. Bicarbonate ion serves as the carbon source for photo-
synthesis. By providing the components needed for photosynthesis (light, CO2, and H20),
oxygen will be produced in the leaf. As photosynthesis takes place, oxygen is released
into the interior of the leaf which changes the buoyancy and causes the disks to rise.
Since cellular respiration takes place and also consumes oxygen, the rate that the disks
rise is an indirect measurement of the net rate of photosynthesis.

Respiration, which uses the oxygen produced by photosynthesis, is also observed in the
leaf disk assay. Some of the oxygen will be used in the leaf’s respiration process. Leaf disks
float, because the net result is that more oxygen is produced by photosynthesis than is
used in respiration. In this investigation, the rate at which leaf “disks” rise will be used as
an indirect measure of the net production of oxygen produced by photosynthesis.

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 EX PERIMENT

AP05 Photosynthesis

Experiment Overview and General Instructions

EXPERIMENT OBJECTIVE:
In this experiment, students will learn how to measure the rate of photosynthesis indi-
rectly using the floating leaf disk method. They will also investigate several factors that
might affect the photosynthesis process.

LABORATORY SAFETY GUIDELINES

1. Wear gloves and goggles while working in the laboratory.

2. Exercise caution when working in the laboratory – you will

Experiment Procedure

be using equipment that can be dangerous if used incor-

rectly.

3. DO NOT MOUTH PIPET REAGENTS - USE PIPET PUMPS.

4. Always wash hands thoroughly with soap and water after working in the laboratory.

5. If you are unsure of something, ASK YOUR INSTRUCTOR!

LABORATORY NOTEBOOKS:
Scientists document everything that happens during an experiment, including experi-
mental conditions, thoughts and observations while conducting the experiment, and, of
course, any data collected. Today, you’ll be documenting your experiment in a laboratory
notebook or on a separate worksheet.

Before starting the Experiment:

• Carefully read the introduction and the protocol. Use this information to form a
hypothesis for this experiment.
• Predict the results of your experiment.

During the Experiment:

• Record your observations.

After the Experiment:

• Interpret the results – does your data support or contradict your hypothesis?
• If you repeated this experiment, what would you change? Revise your hypothesis to
reflect this change.

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 E XP E RIME N T

Photosynthesis
AP05
Investigation I: Observation of Cellular Photosynthesis

Notes:

• For Module I, you will receive 2 plastic cups – (1) cup containing water / liquid
soap solution, and (1) cup containing the bicarbonate / liquid soap solution.
• Throughout this module, you will be preparing material for the “Light -
Control soln” and “15 cm Light - CO2 soln” simultaneously. Follow the steps
below for preparation of materials.

Procedure

1. Label the plastic cup containing water / liquid soap solution provided by your

Experiment Procedure
lab instructor as “Light - Control soln.” This is your control cup.
Fig. 1 - Making leaf disks.
2. Label the plastic cup containing the bicarbonate / liquid soap solution pro-
vided by your lab instructor as “15 cm Light - CO2 soln.” This is your experi-
mental cup.

3. Prepare 10 uniform leaf disks for each trial using the hole punch. Try not to
include the major veins in the leaf disks (Fig. 1).

4. Remove the plunger of the syringe and carefully transfer leaf disks in the
barrel. Shake or tap the barrel on the lab bench to collect the disks to the
bottom (near the opening) of the barrel.

5. Replace the plunger back into the barrel. Push the plunger until only a small
volume of air and leaf disks remain in the barrel (Fig. 2). Be careful not to
damage the leaf disks.

6. Using the transfer pipet provided, put a small volume of sodium bicarbonate
solution (4-5 cc) into the syringe. Gently shake or tap the syringe to suspend
the leaf disks in the solution.
Fig. 2 - Placing leaf disks into
7. While placing a finger over the syringe opening tightly, draw back slowly on the syringe.
the plunger to create a vacuum and hold it for 10 seconds (Fig. 3).

8. While holding the vacuum, swirl the leaf disks to suspend them in solution.
Turn the syringe upright and slowly let the plunger spring back to release the
vacuum.

9. If the disks don’t sink, repeat steps 7-8. You may

have to repeat this procedure two to three times
in order to get all the disks to sink to the bottom
(near the opening) of the syringe barrel (Fig. 4).

Fig. 3 - Creating vacuum in Fig. 4 - Sinking leaf disks.

the plunger.

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 EX PERIMENT

AP05 Photosynthesis

Investigation I: Observation of Cellular Photosynthesis, continued

10. Once all the disks have sunk, remove plunger from the barrel. Swirl and
quickly pour the disks and the solution into the cup labeled “15 cm Light -
CO2 soln“ (Fig. 5).

If the disks stick to the side of the syringe, add a small amount of Sodium
Bicarbonate Solution into the syringe. Slowly swirl the syringe to dislodge
the disks and pour it into the plastic cup (or beaker).

11. Repeat steps 3 – 9 for the cup labeled “Light – Control soln.” Remember
to replace the bicarbonate solution with the diluted soap solution in the
Fig. 5 - Sinking leaf disks
plunger.
in bicarbonate solution.
Experiment Procedure

12. Place both cups under light located about 15 cm away and begin timing
(Fig. 6).

13. Use Table 1 to record the number of disks that are floating in the “Light –
Control soln” cup at the end of each minute (Fig. 7).

14. Use Table 2 to record the number of disks that are floating in the “15 cm
Light - CO2 soln“ at the end of each minute.

15. Continue to record the number of floating disks at the end of each minute
until all of the disks are floating or you have reached 30 minutes.

Fig. 6 - Placing cup under

light source.

Fig. 7 - Disks floating in

bicarbonate solution.

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 E XP E RIME N T

Photosynthesis
AP05
Investigation I: Observation of Cellular Photosynthesis, continued

Table 1: Table 2: 15 cm
Light – Control Solution Light – Co2 Solution

Minutes # of leaf Minutes # of leaf

disks floating disks floating

1 1
2 2
3 3

Experiment Procedure
4 4
5 5
6 6
7 7
8 8
9 9
10 10
11 11
12 12
13 13
14 14
15 15
16 16
17 17
18 18
19 19
20 20
21 21
22 22
23 23
24 24
25 25
26 26
27 27
28 28
29 29
30 30

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 EX PERIMENT

AP05 Photosynthesis

Investigation II - Observation of Plant Respiration

Notes:
Question:
What happens if • For Investigation II, you will receive 1 plastic cup containing the bicarbonate / liquid
photosynthesis is soap solution
not occurring, but
cellular respiration Procedure
continues?
1. Label the plastic cup containing bicarbonate / liquid soap solution provided by your
lab instructor as “30 cm Light - CO2 soln.”

2. Prepare 10 uniform leaf disks using the hole punch. Remember to avoid the major
Experiment Procedure

veins in the leaf when making leaf disks.

3. Remove the plunger of the syringe and carefully transfer your leaf disks in the barrel.
Shake or tap the barrel on the lab bench to collect the disks to the bottom (near the
opening) of the barrel.

4. Replace the plunger back into the barrel. Push the plunger until only a small volume
of air and leaf disk remains in the barrel. Be careful not to damage the leaf disks.

5. Using the transfer pipet provided, put a small volume of sodium bicarbonate solution
(4-5 cc) into the syringe. Gently shake or tap the syringe to suspend the leaf disks in
the solution.

6. While placing a finger over the syringe opening tightly, draw back slowly on the
plunger to create a vacuum and hold it for 10 seconds.

7. While holding the vacuum, swirl the leaf disks to suspend them in solution. Turn the
syringe upright and slowly let the plunger spring back to release the vacuum.

8. If the disks don’t sink, repeat steps 7-8. You may have to repeat this procedure two to
three times in order to get all the disks to sink to the bottom.

9. Once all the disks have sunk, remove plunger from the barrel. Swirl and quickly pour
the disks and the solution into the cup containing bicarbonate / liquid soap solution.

If the disks stick to the side of the syringe, add a small amount of Sodium Bicarbonate
Solution into the syringe. Slowly swirl the syringe to dislodge the disks and pour it
into the plastic cup (or beaker).

10. Place the cup under light located about 30 cm away and begin timing (Fig. 6).

11. Use Table 3 to record the number of disks that are floating at the end of each minute
until all of the disks are floating or you have reached 30 minutes.

12. Once all the leaf disks have floated, remove the cup from the light source and place
it in the dark. Suggestions include covering the beaker with an empty box or a piece
of aluminum foil. Use Table 4 to record how many disks are still floating at the end
of each minute over the next 15 minutes.

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 E XP E RIME N T

Photosynthesis
AP05
Investigation II - Observation of Plant Respiration

Table 3: 30 cm Table 4:
Light – Co2 Solution Dark – Co2 Solution

Minutes # of leaf Minutes # of leaf

disks floating disks floating

1 1
2 2
3 3

Experiment Procedure
4 4
5 5
6 6
7 7
8 8
9 9
10 10
11 11
12 12
13 13
14 14
15 15
16 16
17 17
18 18
19 19
20 20
21 21
22 22
23 23
24 24
25 25
26 26
27 27
28 28
29 29
30 30

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 EX PERIMENT

AP05 Photosynthesis

Data Collection and Analysis

Graph your results for each of the trials on the graph paper provided. Graph the results
from your class average.

a. Label the independent variable (horizontal x-axis).

b. Label the dependent variable (vertical y-axis).
c. Title the Graphs
d. ET50light is the point at which 50% of leaf disks are floating (the median). Find the
ET50light value of each trial (if applicable).
e. ET50resp is the time for 50% to sink after the leaf disks were transferred to the dark
conditions. Determine the ET50resp value.
f. Because respiration occurs in both the light and dark, the rate of photosynthesis
Experiment Procedure

(ETps) is the sum of the rate in the light plus the respiration rate.

1/ET50ps = 1/ET50light + 1/ET50resp

What is the rate of photosynthesis for your experiment?

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 E XP E RIME N T

Photosynthesis
AP05
Data Collection and Analysis

Experiment Procedure

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 EX PERIMENT

AP05 Photosynthesis

Study Questions

1. What is the function of the sodium bicarbonate in this experiment?

2. Explain the process that causes the leaf disks to rise.

3. Explain the process that causes the leaf disks to sink.

4. What is the purpose of using water/soap solution for one of the trials?

5. What is the effect of darkness on photosynthesis? Explain.

6. How does light intensity affect the rate of photosynthesis?

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 E XP E RIME N T

Photosynthesis
AP05
Instructor’s
Guide
Notes to the Instructor & Pre-Lab Preparations
Overview of Laboratory Investigations

The “hands-on” laboratory experience is a very important component of

science courses. Laboratory experiment activities allow students to iden-
tify assumptions, use critical and logical thinking, and consider alterna-
tive explanations, as well as help apply themes and concepts to biological
Order processes.
Online
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Visit our web site for information laboratories conducting biotechnology research. Some of the experimental
about EDVOTEK's complete line procedures may have been modified or adapted to minimize equipment
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tory to the classroom setting. Furthermore, the experiments allow teachers
and students the flexibility to further modify and
adapt procedures for laboratory extensions or
alternative inquiry-based investigations.
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Please have the following
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• Experiment number and title in this section, a variety of re- sources are con-
• Kit lot number on box or tube tinuously being added to the EDVOTEK web site.
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 EX PERIMENT

AP05 In s truct or ’s
Photosynthesis

Guide

Pre-Lab Preparations

A. PREPARATION OF THE DILUTED LIQUID SOAP SOLUTION

1. Add all of the of liquid soap into a beaker containing 2 L of tap water. Let it stir gen-
tly to avoid suds.

2. Dispense 140 ml of the diluted liquid soap solution into 10 plastic cups.

3. Label these cups “Control soln.” Distribute one cup per group.

B. PREPARATION OF THE BICARBONATE SOLUTION

1. Transfer the entire amount of bicarbonate powder to a beaker or flask containing
Instructor’s Guide

2.5 L of tap water. Mix well.

2. Add 500 ml of diluted liquid soap solution (prepared above). Mix well.

3. Dispense 140 ml of the soap/bicarbonate solution into 20 plastic cups.

4. Label these cups “CO2.” Distribute two cups per group.

Each student group will receive:

• One “Control soln” cup containing the water/di-

luted soap solution
• Two “CO2” cups containing the soap/bicarbonate
solution
• One plastic syringe

Reagents to be shared among student groups:

• Leaves (i.e. spinach, ivy, pokeweed)

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 E XP E RIME N T

Photosynthesis
AP05
Inst r uct or ’s
G uide

Experiment Results and Analysis

Graph 1 Result - In this graph, the cup with its floating disks (spinach) was placed under
the light source 15 cm away. The number of floating disks was recorded at the end of
each minute until all of the disks were floating.

Graph 1 - Disks floating in 15 cm Light - CO2 cup during 30 minutes

25

20
Number of leaf disks

15

Instructor’s Guide
Number of
floating disks
10

0
0 5 10 15 20 25 30 35

Time in minutes

a. Label the independent variable (horizontal x-axis): Time in minutes

b. Label the dependent variable (vertical y-axis): Number of floating leaf disks
c. Title the Graph: Disks floating in 15 cm Light - CO2 cup during 30 minutes
By extrapolating the graph above, the ET50light value is 9.5. This means that 50% of
the leaf disks (or 10 leaf disks) were floating at 9.5 minutes.

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 EX PERIMENT

AP05 In s truct or ’s
Photosynthesis

Guide

Experiment Results and Analysis

Graph 2 Result - In this graph, the light was turned off after all the leaf disks have floated for 30 min-
utes. The cup with its floating disks was placed in the dark. Every minute, remove the dark cover and
count the number of leaf disks that were still floating in the next 15 minutes.
Graph 2 - Number of Leaf Disks

25

20
Number of leaf disks

15
Instructor’s Guide

10 Number of
floating disks

0 5 10 15 20 25 30 35 40 45 50
Time in minutes

a. Label the independent variable (horizontal x-axis): Time in minutes

b. Label the dependent variable (vertical y-axis): Number of leaf disks
c. Title the Graph: Disks floating in 30 cm Light - CO2 cup during 30 minutes and disks sinking in
30 cm Light - CO2 cup during 15 minutes
d. By extrapolating the graph above, the ET50light value is 20. This means that 50% of the leaf
disks (or 10 leaf disks) were floating at 20 minutes.
e. By extrapolating the graph above, the ET50resp value is 44 – 30 = 14. This means that 50% of
the leaf disks have sunk after the leaf disks were transferred to the dark conditions.
f. Because respiration occurs in both the light and dark, the rate of photosynthesis (ETps) is the
sum of the rate in the light plus the respiration rate. The rate of photosynthesis (ETps) is calcu-
lated as follows:

1/ET50ps = 1/ET50light + 1/ET50resp

Respiration Rate: ET50resp = 44 min - 30 minutes = 14 minutes

1/ ET50resp = 1/14min = 0.07 min-1

Rate in light: ET50light = 20 min

1/ ET50light = 1/20 min = 0.05 min-1

Photosynthesis Rate: 1/ET50 ps = 1/ ET50light + / ET50resp

1/ET50 ps = 0.05 min-1 + 0.07 min-1 = 0.12 min-1

Photosynthesis Rate: 1.2 x 10-1 min-1

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 Please refer to the kit
insert for the Answers to
Study Questions
 EX PERIMENT
Material Safety Data Sheets
AP05 Full-size (8.5 x 11”) pdf copy of MSDS is available at www.edvotek.com or by request.

Material Safety Data Sheet

May be used to comply with OSHA's Hazard Communication

EDVOTEK ®
Standard. 29 CFR 1910.1200. Standard must be consulted for
specific requirements.

IDENTITY (As Used on Label and List) Note: Blank spaces are not permitted. If any item is not
applicable, or no information is available, the space must
Sodium bicarbonate be marked to indicate that.

Section I
Manufacturer's Name EDVOTEK Emergency Telephone Number
202-370-1500
Address (Number, Street, City, State, and ZIP Code) Telephone Number for information
202-370-1500

1121 5th Street NW Date Prepared

6-12-12
Signature of Preparer (optional)
Washington DC 20001
Section II - Hazardous Ingredients/Identity Information
Hazardous Components Other Limits
(Specific Chemical Identity; Common Name(s)) OSHA PEL ACGIH TLV Recommended % (Optional)

CAS # 144-55-8 No data

Synonyms: Sodium hydrogen carbonate; sodium acid carbonate; baking soda

Section III - Physical/Chemical Characteristics

Boiling Point Specific Gravity (H20 = 1)
N.D. 2.159
Vapor Pressure (mm Hg.) Melting Point
N.D. N.D.
Vapor Density (AIR =1) Evaporation Rate
N.D. (Butyl Acetate =1) N.D.
Solubility in Water
7.8g/100g water @ 18° C (64° F).
Appearance and Odor
Odorless, White crystalline powder
Section IV - Fire and Explosion Hazard Data
Flash Point (Method Used) Flammable Limits LEL UEL

Extinguishing Media
Use any means suitable for extinguishing surrounding fire.
Special Fire Fighting Procedures
Wear SCBA and protective clothing to prevent contact with skin and eyes

Unusual Fire and Explosion Hazards

None

Section V - Reactivity Data

Stability Unstable Conditions to Avoid

Stable X
Incompatiblity (Materials to avoid) Reacts with acids to form carbon dioxide.
Hazardous Decomposition or Byproducts Gaseous carbon dioxide.

Hazardous May Occur

Polymerization
Will Not Occur X
Section VI - Health Hazard Data

Route(s) of Entry: Inhalation? Skin? Ingestion?

Yes Yes Yes
Health Hazards (Acute and Chronic)
Irritation

Carcinogenicity: NTP? IARC Monographs? OSHA Regulation?

Signs and Symptoms of Exposure

Irritation
Medical Conditions Generally Aggravated by Exposure

Emergency and First Aid Procedures

Inhalation: Remove to fresh air. Ingestion: Drink several glasses of water to dilute. If large swallowed, get
medical advice. Skin Contact: Not expected to require first aid measures. Eye Contact: Wash thoroughly
with running water. Get medical advice if irritation develops.
Section VII - Precautions for Safe Handling and Use
Steps to Be Taken in case Material Is Released or Spilled
Ventilate area of leak or spill. Sweep up and containerize for reclamation or disposal. Vacuuming or wet
sweeping may be used to avoid dust dispersal. Small amounts may be flushed to sewer with plenty of water.
Waste Disposal Method
See above
Observe all federal, state, and local laws.
Precautions to be Taken in Handling and Storing
Keep in a tightly closed container, stored in a cool, dry, ventilated area. Protect against physical damage.
Observe all warnings and precautions listed for the product.
Other Precautions

Section VIII - Control Measures

Respiratory Protection (Specify Type)

Ventilation Local Exhaust yes Special

Mechanical (General) Other
Protective Gloves yes Eye Protection Chem safety
Other Protective Clothing or Equipment
Work/Hygienic Practices
Wash thoroughly after handling

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