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11To Study Wing Shape and Eye Colour in Drosphia

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47 views

11To Study Wing Shape and Eye Colour in Drosphia

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thelostmoon61
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© © All Rights Reserved
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11. To study wing shape and eye colour in Drosphila.

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I _I __ j
Alm: To study the genetic variation of traits in Drosphilo melonogoster.
Requirements: Glass jar, petri dish, banana pieces, muslin cloth, bell jar, slide, cover slips,
graded series of alcohol, xylene, DPX, etc.
Figure:

Other
Colour

Wildtype Ebony ·1• (e) Yellow ·1• (y) Antp +i- (Antp)
Eyes

White ·1• (W) Mini white (W+) Bar +i- (B) Drop +i. (Dr) Irregular Facets +I- (if)
wings

Curly +i- (Cy) Serrate +i. (Ser) Vestigial ·1· (vg) Wildtype Dichaete (D)

Humeral +i- (Hu) Pin +i- (Pin) Stubble +i- (Sb) Singed ·1• (sn)
Procedue:
I. Preparation of culture beds
1. Peel the ripe banana fruit. Discard the skin and mash the fruit into a soupy pulp.
2. Take a clean sterilized glass jar or petridish and place the banana pulp in it.
3. Add few grains of yeast.
4. Keep a small blotting paper to absorb excess moisture from the pulp and also to provide
substratum for pupation of the larvae.
5. Many culture beds can be prepared by the above method for the different varieties of fruit fly.
II. The culturing of Drosophila :
6. The pulp in the culture beds attracts the Drosophila. Cover the culture beds with a bell jar after
the Drosophila have reach the pulp. They complete their life cycle within 48 hours.
7. Capture the Drosophila and fix it into 70 % alcohol.
8. Process the Drosophila flies through 70%, 90% and 100% graded series of ethyl alcohol for
10 minutes each.
9. Place Drosophila in Xylene for 5 min. and clear it properly.
10. Mount it on glass slide with DPX and observe under the microscope.
Observation and Result :
Eye Color Pottem of Wing
..
No. of 'segments in o leg
Culture 1 Red(wild) Normal Nine number of segments
Culture 2 Black (mutant) Vestigial Less number

(Questions j
1. Why T.H. Morgan used Drosophila melanogaster in his genetic experimen�s ?
T. H. Morgan used fruit flies for his genetical experiments for the following reasons-
1) Fruit flies have a short life cycle so genetic traits can be studied in many generations in a short span.
2) They reproduce rapidly and produce many offsprings.
3) They have four pairs of polytene chromosomes which are large and can be easily seen under a light
microscope.
2. What are the differences between male and female Drosophila?
Females Males
Body shape Pointed abdomen with a “spike” Rounded abdomen
on dorsal surface at rear
Colour Each abdominal segment Rearmost abdominal segments almost
carries a narrow dark band uniformly dark
Genitalia Few structures visible Complex structures visible on ventral
surface
Sex combs None A short row of thick, closely spaced bristles
appearing as a dark mass on the fourth
segment of the front legs (often seen
best with fly lying on its back)

3. What is linkage ?
The physical association of non-parental gene combinations is called linkage.
4. What kind of eye colour and pattern of wing is present in wild fruit fly?
The wild-type eye color of Drosophila is dull red
Wild type wings have wings that are flat and wide.

5. Is the inheritance of wing type/eye colour in drosophila-multigenic or multiallelic,

the inheritance of wing type/eye colour in drosophila is multigenic

( Multiple Choice Questions j


1. The mechanism of sex determination in Drosophila is_ _ _
_
a. XX - XO type b. XX - XY type
c. ZZ - ZW type d. XX - ZZ type
2. How many different alleles are seen in wing series of Drosophila?
a.3 b.6
C. 5 d.4
3. How many pairs of chromosomes are present in somatic cell ofDrosophila?
a. 7 b. 8
C. 12 d.4
4. Wing shape in Drosophilais an example of____ _
a. co-dominance b. multiple alleles
c. pleiotropy d. incomplete dominance
5. How many linkage groups do occur in female Drosophila?
a.4 b. 8
c.2 d. 7

Re111ork and Sig11ature of Teacher ........................... , ....._...........................................................

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