co2_handout
co2_handout
(LI-6400)
/Petra Majer/
PRINCIPLES
LI-6400 is an open system design photosynthetic gas exchange system. IRGAs (H2O
and CO2) are in the sensor head, that gives a possibility for a tight control of
conditions in the leaf chamber response to leaf changes. The incoming air stream can
be conditioned for CO2 concentration, humidity and temperature. There are chemical
tubes for scrubbing CO2 and H2O, and air can be diverted through these tubes in any
proportion desired. CO2, however, is best controlled by scrubbing all of it from the
incoming air, and using CO2 source to provide a stable concentration at the desired
value (optional CO2 injection system). The system is equipped with Peltier
thermoelectric coolers for temperature control and light sensors. LI-COR offers a
variety of interchangeable leaf chambers (coniferous, broadleaf, soil). The newest
version, LI-6400XT is also capable of measuring fluorescence and gas exchange
simultaneously over the same leaf area.
The reference analyzer measures incoming gas concentrations and is located directly
below the sample analyzer. The sample and reference analyzers can be matched at any
time, either manually or automatically, without altering conditions in the leaf
chamber.
A B
The LI-6400 device (A) and the sensor head (B) (Source: Using the LI-6400, Manual, version 5.3)
Main parts of the LI-6400 device:
Console: a data logging computer with keypad and LCD screen
Sensor Head/IRGA: leaf chamber (for different leaf areas), spring-loaded latching
handle, 2 Peltier thermoelectric coolers, sample and reference IRGAs, light sensor
(external quantum sensor is optional)
Chemical Tubes: a tube containing soda lime (to remove CO2 from incoming air
stream) and a tube containing Drierite, a desiccant (to remove water vapour)
CO2 source: cartridge + injector or tubing from a buffer volume
Amax
(PAR)
Photosynthetic light response curve in a C3 plant (Source: Taiz & Zeiger 2010)
PRACTICAL
Samples:
Tobacco (Nicotiana tabacum L. var Xanthi), 6-week-old
1, greenhouse grown at ca. 200 µmol m2 s-1 PAR
2, greenhouse grown at ca. 200 µmol m2 s-1 PAR and placed outdoor under solar
radiation for a few days
3, greenhouse grown at ca. 200 µmol m2 s-1 PAR, water withdrawal for a few days
(drought stressed)
Measurements:
1, Rapid light response curves (ca. 2 min per irradiation level)
a) greenhouse tobacco
b) outdoor tobacco
c) drought stressed tobacco
2, Effect of clouds
a) „fast cloud” (brief shade simulation)
b) „slow cloud” (long shade simulation)
Long SP, Farage PK, Garcia RL, 1996. Measurement of leaf and canopy photosynthetic CO2 exchange in
the field. Journal of Experimental Botany 47: 1629-1642.
Mulkey SS, Smith M, 1988. Measurement of photosynthesis by Infra Red Gas Analysis. IN:
Bioinstrumentation, C.T. Lange, (ed). 79-84. Publication of the American Biology Teachers Association.
Taiz L, Zeiger E, 2010. Plant Physiology, Fifth Edition. Sinauer Associates, Inc. Sunderland, MA, USA.
LI-COR, 2004. Using the LI-6400 Portable Photosznthesis System. Version 5 (2004) LI-COR
Biosciences, Inc. Lincoln, NE, USA
http://www.licor.com/env/products/photosynthesis/measurements.html
DATA SHEET
Plant PAR A E gs Ci
µmol m-2 s-1 µmol CO2 m-2 s-1 mmol H2O m-2 s-1 mol H2O m-2 s-1 µmol CO2 mol-1
100
300
500
greenhouse 700
tobacco 900
1100
1300
1500
1500
(30 s) 300
1500
(2 min) 300
1300
outdoor
1100
tobacco
900
700
500
300
100
100
300
500
drought
700
stressed
900
tobacco 1100
1300
1500
30 30
A (µmol CO2 m s )
A (µmol CO2 m s )
-1
-1
25 25
-2
-2
20 20
15 15
10 10
5 5
0 0
100 300 500 700 900 1100 1300 1500 100 300 500 700 900 1100 1300 1500
-2 -1 -2 -1
PAR (µmol m s ) PAR (µmol m s )