Botany
Botany
U.P-222001
0
M.Sc.Botany Syllabus VBS Purvanchal University Jaunpur
D.D.U.Gorakhpur
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M.Sc.Botany Syllabus VBS Purvanchal University Jaunpur
SEMESTER - I
Evaluation Duration of
Teaching
Subject Code Subject / Paper Theory / Practical Credits External
hours /Sem. Internal External
Examination
B040702T Theory
Mycology 60 4 25 75 3
(Paper-II)
B040703T Theory
Phycology 60 4 25 75 3
(Paper-III)
B040704T Bryophytes
Theory
And 60 4 25 75 3
(Paper-IV)
Pteridophytes
B040705P Practical
Practical Lab 60 4 25 75 8
(Paper- V)
Minor elective
Industrial Project
B040706R training/Research Report/Dissertation 4 50
60
project/Survey (Paper-Vll)
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M.Sc.Botany Syllabus VBS Purvanchal University Jaunpur
SEMESTER - II
Subject Code Subject / Paper Theory / Practical Teaching Credits Evaluation Duration of
hours/sem. External
Examination
Internal External
Or 4 25 75 3
Elementary Biotechnology
Or
B040804TB
B040805P Practical Lab Practical 60
4 25 75 8
(Paper-V)
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M.Sc.Botany Syllabus VBS Purvanchal University Jaunpur
SEMESTER – III
Biodiversity of (Paper-III) 4 25 75
angiosperm
B040904TB B-Genetic
Engineering
(Paper-VI)
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M.Sc.Botany Syllabus VBS Purvanchal University Jaunpur
SEMESTER - IV
A-Taxonomy Of (Paper-III)
Angiosperm And 60 4 25 75 3
Or
Ethnobotany Or
A-Pharmacognosy (Paper-IV)
Or 60 4 25 75 3
(special)
Industrial Project 50
B041006R training/Research Report/Disser
60 4
project/Survey tation
(Paper-VI)
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Subject Prerequisites: To study this subject a student must have had the subject(s)
Botany at UG Level.
Course Structure: The courses will be based on Choice Based Credit System
(CBCS) structure developed by the University. There will be four compulsory or
elective core coursesof Botany in each semester. Apart from these, one minor
elective course of other faculty is to be chosen by a student in the first year of
M.Sc. (Botany). In each semester, there will be one research project of 04 credits.
This programme has been designed to train and enable students to understand the
relationship between science and society as well as logical, scientific and ethical issues
related to science. In addition to this, the students will be able to think critically for the
formulation of hypotheses and experimental designing based on the scientific method, which
will make the students readily employable in various streams of teaching, research, civil
services and in industries.
Programme Specific Outcomes (PSOs): After completing M.Sc. (with Botany), the following
will be the PSOs
PSO-1: It is expected that after successfully completing M.Sc. Botany, students will
develop deeper theoretical & Practical knowledge of different branches of Botany like
Phytotecnique, Plant taxonomy, Anatomy, Mycology, Microbiology, Physiology,
Biochemistry, Cell biology, Genetics, Molecular biology, Medicinal Botany,
Pharmacognosy, Environmental issues etc, making them capable of understanding the
societal, environmental issues, demands and their solutions.
PSO-2: This program has a strong theoretical basis that will help students in evolutionary
relationship of lower and higher plants by using the key characters which is expected from a
student of Botany to support the other branches of knowledge related to plants.
PSO-3: Many of the courses in the programme have been carefully designed that will help the
students for qualifying competitive exams like IAS, IFS, CSIR NET, SET, TGT, PGT and
to write research proposals for grants.
PSO-4: Continuous internal assessment provides ample opportunity to the students for
improvement after every evaluation. Seminar and field visits system grooms the personality
of the students and enables them to present oneself with confidence, develop a reasonably
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
well compiled content and discuss. Assignments enable the students to compile the
solutions of the given problems with optimal discussion.
PSO-5: In each semester of the programme, each student is given research project of their own
choice to allow students to understand various steps of solving a research problem. Thus,
this programme will help to develop research aptitude at PG level with identification of
gaps in knowledge and relevance oftheir solutions for the society.
PSO 6. The student completing the course will be capable of executing research projects.
The types of paper, number of papers, credit for each semester and research project
semester wise, as per guidelines of UP state govt. letter no. 401/sattar-3-2022, dated
February 09, 2022, as a part of NEP-2020 implementation in U.P. universities, have been
prepared as above.
The maximum and minimum marks for each Theory paper, Practical papers
(internal & external) and projects have been finalized according to the letter
number-1032/sattar-3-2022-8(35)/2020, dated 9 February, 2022. The basic
structure of the programme related to types of paper, number of papers, credit etc
have been finalized according to the letter of government dated July 13, 2021.
Core Compulsory Courses: These are main (major) courses of the subject
which every student has to study who has taken admission in PG (First and
Second Year).
Core optional (specialization) Courses: These are full major courses of the
subject/programme. There courses will selected by the studentsin 1/2/3/4 (or
7/8/9/10 in case of integrated PG) Semesters. The Botany department of
university/colleges will run these courses in their department/colleges according
to their resources/ specialization of teacher and students may opt them according
to their choice.
Minor Electives: Some of the above courses, or any other such course
developed by BoS, can be taken as Minor electives by the students of other
Faculty, for multi-disciplinarily.
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Research Project:
B.Sc. IV year will be equivalent to M.Sc. 1st year. At the end of the 2nd(even)
semester (B.Sc. VIII semester or MSc IInd semester) & 4th/10th semester
(M.Sc.), the candidate will submit a research project, which will be evaluated by
an external examiner & internal supervisor along with a presentation and viva-
voce examination.
In Fourth year (B.Sc.) or MSc first year and Fifth year (MSc final) the topic of
the research project will be chosen from among the core compulsory
courses/core elective courses of that year.
In each semester, each student will work 4 hour/ week/ semester for 4 credit. In
this way a project work will be of 8 credits (i.e. 16 credits for two years).
Research project may be interdisciplinary/ multidisciplinary. It may be an
industrial training/ internship/ survey work. Research project will be done
under the guidance of the faculty member (s) preferably having PhD degree.
For this a co-supervisor may be chosen from a university, college, industry,
research institute etc.
The research project will be of 100 marks. If any student publishes a research paper from
his/her research project in a UGC care listed/ Scopus indexed or Thomsom rueter, then
he/she will get 25 extra marks (although maximum marks will not exceed more 100). The
marks obtained in research project will be coded in grades and they will be counted in the
calculation of CGPA.
Credits: MSc Programme will be run semester wise and choice based credit system.
MSc Ist year or B.Sc 4th year will be of 52 credits whereas MSc 2nd year will be
of 48 credits. Each semester will be of 20 credits of courses (4 theory+1 practical,
each will be of 4 credits) and thus the credits of two years (4 semesters) will be
80 credits.
A project work will be of 8 credits (i.e. 16 credits for two years). In other words
it will be of 4credits/semester i.e. a total of 16 credits.
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
A minor elective will be of 4 credits.
Thus M.Sc Programme will be of 100 credits (52+48).
Semester Ⅰ St
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
PAPER-Ⅰ: Microbiology
Unit-Ⅰ
Lichens- A general account of lichens and its symbionts, thallus structure, physiology, reproduction,
classification and chemistry of lichens, isolation of symbionts, synthesis of food and economic
importance.
Contribution Of Indian Botanist among different stream in Life Sciences- Professor M.O.P.
Iyengar,Professor K.C.Mehta, Professor Shiv Ram Kashyap, Professor Birbal Sahni and
Professor Hargovind Khorana
Unit-Ⅱ
Viruses- Nature of plant viruses and symptoms caused by them, microscopic, histological and
cytological,Transmission of plant viruses, their relationship with vectors
Unit-Ⅲ
Unit-Ⅳ
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Reference Books-
Change. S.T. and P.G. Miles - Edible mushrooms and their cultivation
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
PAPER-Ⅱ :Mycology
Unit-Ⅰ
Status of fungi, principles of important systems of classification of fungi upto the rank of classes, Detailed study of the
classification of Alexopoulos and Mims 1970.
Unit-Ⅱ
Classification upto the rank of order. Range of structure and organisation of vegetative and reproductive bodies. Ultra
structure of fungal cells. Method of reproduction. Variations in life-cycle.
Unit-Ⅲ
Modes of nutrition of fungi and their physical and chemical requirement for growth and reproduction.
Unit-Ⅳ
b.Harmful activities, Deterioration of materials by fungi, Fungi as agents of Plant and Human diseases.
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Reference Books-
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
UNIT - I
General characters and comparative study of important systems of classification of algae – Fritsch system of
classification.
Criteria used in the primary classification of algae: a) Pigments b) Reserve food materials c) Flagella d) Cell wall
e) Range of the organization.
Algae of diverse habitats – a) Terrestrial b) Freshwater algae and c) Marine algae d)Gross Cell Structure
UNIT – II
General characters, morphology, life history and classification of the followinggroups of algae:
Cyanophyceae – Chrococcus,Gloeotrichia,Nostoc,Cylendrospermum,Stigonema,Cephaleuros.
Charophyceae - Nitella
UNIT-III
General characters and morphology, life history of the following groups of algae.
Xanthophyceae – Botrydium,Vaucheria
Dinophyceae-Peridinium,Ceratium
UNIT-IV
Algal blooms and Toxic algae, Algal biofertilizers, Algae as Food and Feed. Role of algae in industry (Alginic
acid, Agar, Carrageenan)
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Reference Books-
Bold, H.C. and Wynne, M.D.: Introduction to the Algae structure and reproduction
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
UNIT - I
Classification systems of Bryophytes Distribution, structure and reproduction of the following groups:
Marchantiales; Marchantiaceae-Marchantia, Targionia, Jugarmanniales- Porella,Anthocerotales- Anthoceros, Notothyllas
,Sphagnales – Sphagnum,Polytrichales- Polytrichum , jungermaniles
UNIT - II
UNIT - III
UNIT- IV
Telome theory & its application,Heterospory & seed habit,Geological time scale,Process of fossalization, and
techniques employed in types of fossils,Origin and evolution of early vascular plants,General characters of
Lepidodendrales, Calamitales and Sphenophyllales.
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Reference Books-
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Unit-I -Microbiology
Introduction to basic Mycrobiological Techniques and Lab. Safety; Methods of sterilization, media preparation and culturing.
Unit - II -mycology
Identification of fungal cultures, slides and specimens of Synchytrium, Allomyces, Glomus, Emericella, Neurospora,
Morchella, Fusarium, Colletotrichum, Melampsora, Phallus, Ustilago, Peronospora, and Stemonitis.
Study of Symptomology of the following fungal diseases by taking sections and slide preparation: Downy mildews, Tikka
disease, Melampsora rust, Wheat rust and White rust.
Identification of fungal cultures, slides and specimens of Rhizopus/Mucor, Aspergillus, Penicillium, Yeast, Fusarium,
Alternaria, Cercospora, Pythium, , VAM fungi, Trichoderma. Study of Mycorrhizal colonization in roots of Parthenium and
Study of Mushroom specimens.
Unit - III-Phycology
Identification of the genera mentioned in Cyanophyceae and Chlorophyceae. Collecton and identification of algae
occurring in and around university college/campus . .
Identification of bloom forming algae. Identification of Algal biofertilizers. Identification of toxic algae.
Plagiochasma
Targionia
Notothylas
Sphagnum
Pteridophyta, Morphology and anatomy of vegetative and reproductive organs using cleared whole mount sections. Macerations
and permanent preparation of Psilotum, Isoetes, Ophioglossum, Adiantum, Salvinia, Azolla.
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Course Objectives: The objective of this course is to provide students with hands-on training in
specialized areas of plant sciences
Course Outcomes: After completion of this course the students will acquire the following:
1. Training in experimental design and execution
2. Knowledge on techniques and tools of research
3. Designing of research proposal.
4. Writing of research and review paper
Contents
The topic of the research project will be chosen from among the core compulsorycourses/core
elected courses of that year.
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
nd
Semester II
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
UNIT – I
Distribution of Gymnosperms - Past and present. Classification of Gymnosperms – Proposed by Sporne and Pant.
UNIT – II
A general account of Gymnosperms with reference to their vegetative morphology and anatomy and male and
female cones of the following taxa). a)Cycadales (Cycas, Zamia) b). Ginkgoales (Ginkgo)c). Coniferales (Araucaria,
Podocarpus, Cupressus and Cedrus) d)Taxales (Taxus) e).Gnetales (Ephedra, Welwitschia)
UNIT – III
Microsporangium: Anther, sporogenous tissue, formation of pollen wall, vegetative and generative nucleus.
Megasporangium: Ovule, types of ovules, Nucellus, Megasporogenesis, embryosac types, a special account of mature
embryosac.
UNIT-IV
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Reference Books-
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Unit -I
The Environment: Physical environment; biotic environment; biotic and abiotic interactions.Habitat and Niche: Concept
of habitat and niche; niche width and overlap; fundamental and realized niche; resource partitioning; character
displacement.
Population Ecology: Characteristics of a population; population growth curves; population regulation; life history
strategies (r and K selection); concept of metapopulation – demes and dispersal, interdemic extinctions, age structured
populations.Species Interactions: Types of interactions, interspecific competition, herbivory, carnivory, pollination,
symbiosis.
Unit- II
Community Ecology: Nature of communities; community structure and attributes; levels of species diversity and its
measurement; edges and ecotones.Ecological Succession: Types; mechanisms; changes involved in succession; concept
of climax.
Unit- III
Ecosystem Ecology: Ecosystem structure; ecosystem function; energy flow and mineral cycling (C,N,P); primary
production and decomposition; structure and function of some Indian ecosystems: terrestrial (forest, grassland) and
aquatic (fresh water, marine, eustarine).Biogeography: Major terrestrial biomes; theory of island biogeography;
biogeographical zones of India.
Unit-ⅤI
Applied Ecology: Environmental pollution; global environmental change; biodiversity: status, monitoring and
documentation; major drivers of biodiversity change; biodiversity management approaches.Conservation Biology:
Principles of conservation, major approaches to management, Indian case studies on conservation/management strategy
(Project Tiger, Biosphere reserves).
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Reference Books-
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Course Code – B040803T Course Title -Taxonomy of Angiosperms and Medicinal Botany
Paper- III - Taxonomy of Angiosperms and Medicinal Botany
UNIT -I
1. Systems of classification: Phenetic and Phylogenetic systems. Critical account of the system of
classifications of a) Hutchinson b) Cronquist and c) Takhtajan.
2. Taxonomic evidence and techniques used therein a) Morphology b) Micromorphology c)
Epidermology d) Cytology e) Phytochemistry f) Nucleic acid hybridization.
UNIT -II
3. Nomenclature: a) Concept of ICBN b) Salient features of Botanical Nomenclature c) Ranks and
Nomenclature of taxa d) Typification e) Rules of Priority f) Effective and valid publication g) Author
citations.
4. Biosystematics: a) Concept b) Categories c) Species concept
UNIT -III
5. A comparative study of the following pairs of families and their treatment in recent
systems:
a) Magnoliaceae & Winteraceae
b) Malvaceae & Sterculiaceae
c) Rutaceae & Meliaceae
d) Apocynaceae & Asclepiadaceae
e) Verbenaceae &Lamiaceae
f) Amaranthaceae & Chenopodiaceae
g) Cyperaceae & Poaceae
6. Origin of angiosperms, with reference to recent findings.
UNIT -IV
7. Medicinal Botany:
a) Role of plants in medicine, its origin and development
b) Morphology, active principles and medicinal value of the following:
i. Andrographis paniculata
ii. Asparagus racemosus
iii. Clitoria ternatea
iv. Phyllanthus emblica
v. Gymnema sylvestre
8. Flora of U.P.: Salient features of vegetational aspects.
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Reference Books-
1. Lawrence: Taxonomy of Vascular Plants
2. Sivarajan, V.V. (Ed. Robson). Introduction to the Principles of Plant Taxonomy
3. Heywood, V.H. Plant Taxonomy
4. Naik, V.N. Taxonomy of Angiosperms (1988)
5. Stace, C.R. Plant Taxonomy and biosystematics (2nd Ed.)
6. Hutchinson, J. The families of flowering plants (3rd Ed.), 1973
7. Takhtajan, K. Outline of classification of flowering plants. Botanical Rev. 46:225-359),
1980
8. Flowering plants. Origin and Dispersal (Trans. By Jeffry), 1969
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Course Code – B040804T A Course Title - Cell Biology, Genetics and Biostatistics
Paper- IV - Cell Biology, Genetics and Biostatistics
UNIT -I
2. (Structure of model membrane, lipid bilayer and membrane protein diffusion, osmosis, ion channels, active transport,
membrane pumps, mechanism of sorting and regulation of intracellular transport,electrical properties of membranes).
3. Structural organization and function of intracellular organelles (Cell wall, nucleus, mitochondria, Golgi bodies,
lysosomes, endoplasmic reticulum, peroxisomes, plastids, vacuoles, chloroplast, structure & function of cytoskeleton
and its role in motility).
4. Organization of genes and chromosomes (Operon, unique and repetitive DNA, interrupted genes, gene families, structure
of chromatin and chromosomes, heterochromatin, euchromatin, transposons).
5. Cell division and cell cycle (Mitosis and meiosis, their regulation, steps in cell cycle, regulation and control of cell cycle).
UNIT –II
Mutations: Gene mutations (substitutions and frame-shift mutations), Chromosomal aberrations (structural), Transposon-
induced mutations; Site-directed mutagenesis.
Inherited human diseases: Haemophilia and Sickle cell Anaemia. Gene therapy
UNIT –III
Brief account of plant tissue culture, micro propagation and transgenic plants. Overview of recombinant DNA technology.
Gene cloning, genomic / cDNA libraries, restriction mapping, blotting methods, polymerase chain reaction and DNA
fingerprinting. Brief overview of plant breeding methods: Conventional, mutation breeding, QTLs and MAS.
UNIT –IV
Mean, Variance, Standard deviation and Standard error.Chi-square and Student’s “t” test. Probability distribution
(Binomial, Poisson and Normal). Introduction to computers. Use of Word and PowerPoint in the preparation and
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Reference Books-
G. M. Cooper. 1997. The Cell and Molecular approach. ASM Press. Ed.
Prathibha Devi. Principles & Methods in Plant Molecular Biology, Genetics&Biochemistry, Agrobios.
Stansfield. 1996. Theory & Problems in Genetics. Schaum’s Series. McGraw & Hill.
OR
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Unit-I
2.Recombinant DNA technology, basic concept in genetic engineering, tool and techniques of recombinant
DNA technology.
4.Cloning vehicles: Plasmids, Cosmids, Lambda phage, Charon phage, shuttle vectors, 2µ DNA plasmids, yeast
plasmids, M13 vector. Fermentation and enzyme technology
Unit-II
Unit-III
1-Selection of genes, Gene libraries, Genomic and cDNA library - Gene transfer methods, Genetic
organization of Ti plasmids, Ti plasmid mediated transfer - Agrobacterium tumifaciens, DNA mediated
transfer.
2-Calcium phosphate, PEG, DEAE, via liposomes - Microinjection -Macroinjection, microprojectile, and
electroporation, - Selection of clones,marker and reporter genes in screening methods.
Unit-IV
2.Use of transposons in genetic analysis: Transposon tagging and its use in identification and isolation of genes.
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Reference Books-
1.Tropp, B. E. (2012). Molecular Biology. Fourth Edition, Jones and Bartlett India Pvt. Ltd, New Delhi.
2.Howe, C., (2007). Gene Cloning and Manipulation. 2nd Edition.
3.Watson, D., Baker, T. A., Bell, S. P., Gann, A., Levine, M., and Losick, R. (2008). Molecular Biology of
Gene. 6th Edition, Cold Spring HarborLaboratory Press Cold Spring Harbor, New York, U.S.A.
4.Clark, D., Pazdernik, N., McGehee, M. (2018). Molecular biology. 3rd Edition.
5.Freifelder, D. (1990). Molecular Biology. 2ndEdition,Narosa Publishing HouseNew Delhi.
6.Nicholl, D. S. T. (2008). An Introduction to Genetic Engineering. 3rd Edition.
7.Plant Molecular Biology - Genetic Analysis of Plant Development and Metabolism. Springer-Verlag, New
York, London.
8.Grierson, D. and Covey, S. (1984). Plant Molecular Biology, Practical Approach. IRL Press, Oxford,
Washington DC.
9.Henry, R. J. (2005). Practical Applications of Plant Molecular Biology. Chapman & Hall, London, UK.
10.Shaw, C. H. and Brown. T.A. (1988, 2020). Gene Cloning and DNA Analysis: An Introduction. 8th Edition.
11.Primrose, S. B. and Twyman, R. (2006). Principles of Gene Manipulation and Genomics. 7th Edition.
12.Tewari, K. K. and Singhal, G. S. (1997). Plant Molecular Biology and Biotechnology. Narosa Publishing
House, New Delhi.
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
UNIT- I
1-Gymnosperms: Comparative study of the vegetative, reproductive parts and Anatomy of the following: Zamia,
Araucaria, Cedrus, Thuja, Ginkgo and Taxus.
2-Embryology: Study of embryology by specimens and slides.T.S. of anther.Study of ovules by hand section.Globular
embryo Mature embryo .Polyembryony.Pollen viability.
1. Study of the locally available plants and recording of the intraspecific variation.
2. Description and identification at family, genus and species levels using Floras.
3. Identification of key characters in a group of species of a genus and construction of keys.
4. Construction of indented keys for the given material
5. Simple Nomenclatural problems
6. Identification of families studied based on flowers or essential parts of the flowers
7. Knowledge of Herbarium techniques
8. Record and Herbarium
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
OR
3. Organ culture
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Course Objectives: The objective of this course is to provide students with hands-on training in specialized
areas of plant sciences
Course Outcomes: After completion of this course the students will acquire the following:
5. Training in experimental design and execution
6. Knowledge on techniques and tools of research
7. Designing of research proposal.
8. Writing of research and review paper
Contents
The topic of the research project will be chosen from among the core compulsorycourses/core
elected courses of that year.
34
M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
rd
Semester III
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
UNIT -I
Composition, structure and function of biomolecules (carbohydrates, lipids, proteins, nucleic acids and vitamins).
Stablizing interactions (Van der Waals, electrostatic, hydrogen bonding, hydrophobic interaction,etc.).
Principles of biophysical chemistry (pH, buffer, reaction kinetics, thermodynamics, colligative properties).
UNIT –II
Bioenergetics, glycolysis, oxidative phosphorylation, coupled reaction, grouptransfer, biological energy transducers.
Principles of catalysis, enzymes and enzyme kinetics, enzyme regulation, mechanism of enzyme catalysis, isozymes
UNIT-III
UNIT-IV
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Reference Books-
Plant Physiology, biochemistry and molecular biology. David, T: Dennis and Davis Turnip. Longman. Scientific and
technical U.K. 1990.
Outlines of biochemistry. 5th edition Con E.E. and Stump P.K. 1995. Willey
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
UNIT –I
Photosynthesis - Light harvesting complexes; mechanisms of electron transport; photoprotective mechanisms; CO2
fixation-C3, C4 and CAM pathways.
Respiration and photorespiration – Citric acid cycle; plant mitochondrial electron transport and ATP synthesis; alternate
oxidase; photorespiratory pathway.
UNIT –II
Plant hormones – Biosynthesis, storage, breakdown and transport; physiological effects and mechanisms of action.
UNIT-III
Sensory photobiology - Structure, function and mechanisms of action of phytochromes, cryptochromes and
phototropins; stomatal movement; photoperiodism and biological clocks.
Solute transport and photoassimilate translocation – uptake, transport and translocation of water, ions, solutes and
macromolecules from soil, through cells, across membranes, through xylem and phloem; transpiration; mechanisms of
loading and unloading of photoassimilates.
UNIT-IV
Secondary metabolites - Biosynthesis of terpenes, phenols and nitrogenous compounds and their roles.
Stress physiology – Responses of plants to biotic (pathogen and insects) and abiotic (water, temperature and salt) stresses.
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Reference Books-
Plant Physiology by F.B. Salisbury and C.W. Ross. Wordsworth biology series.
Plants Cell structure and metabolism. J.L. Hall, Flower and Roberts, ELBS, Longman.
Introduction to Plant Physiology by G.R. Noggle and G.J. Fritz, Printice Hall Press
Introduction to plant physiology W.G. Hopkins and Norman P.A. Huner, Plant Physiology. Lincoln Taiz and Eduardo Zeiger
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
UNIT –I
Concept of Biodiversity, its origin and development .a) Definition b) Past history c) Ranks recognized in Biodiversity
studies, taxonomy and others d)Keystone taxa.,Aims and objectives of Biodiversity ,Characterization of Biodiversity
UNIT –II
Magnitude and Distribution of Biodiversity,Current magnitude of Global Biodiversity,Botanical regions and Hotspots
,Distribution of Biodiversity,Endemism and Biodiversity,Degeneration Maintenance and Loss of Biodiversity,Diversification
of species,Ecological extinctions,Proximate causes
UNIT –III
Inventorying: Definition, purpose, orientation, completeness and intensity. Indicator selection for Biodiversity inventory.
Monitoring of Biodiversity at different biological levels: Genetics, Population level and Species level; Species turnover in
Ecosystems-Landscape levels.
Monitoring:
Inventorying and monitoring for conservation: RAMSAR convention, sites, Red data (booksand lists).
UNIT –IV
Economic value and utilization of Biodiversity with reference to the following taking five examples for each: a) Food b)
Fodder c) Fibre d) Oils e) Drugs f) Timber g) Rubber h) Spices I) Essential oils j) Gums and Resins k) Insecticides and
Pesticides l) Ornamentation
A brief account of origin of cultivated plants,Biodiversity convention a) Initiative from UN b) Rio Conference c) Recent
efforts,Conservation of Biodiversity
Reference Books-
Biodiveirsity and ecosystem function.Ed.B7 Schulze, ED and Mooney, HA Springer Verlag. NY. 1996.
Functional roles of Biodidversity: A Global Perspective. Mooney, HA, Cushman, JH, Miduo, E, Sale, OE and Schulze,
ED. 1995.
Biodiversity prospecting: Using Genetic resources for suitable development. Reid et al.WRI, USA, 1993.
Conserving Biodiversity for suitable development, Ramakrishnan, AK.Das and Saxena INSA, N.Delhi. 1995.
Biodiversity and Forest Genetic Resources. D.N. Tewari. International Book Distrib.Dehradun
Biodiversity in Managed landscapes. Theory and practice. R.C. Szatro and D.W. Johnston.Oxford University Press. 1996.
General Ecology. HD. Kumar. Vikass Publ. House Pvt. Ltd. 1995.
Kumar, U – Biodiversity
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
UNIT -I
Importance of active principles and uses of medicinal plants in different traditional systems of medicine and Allopathy
Origin, Historical background. Active principles uses and cultivation practices of the following medicinal plants
Andrographis paniculata b) Asparagus racemosus c) Bacopa monnieri d) Coleus forskohlii e) Rauwolfia serpentine f
)Withania somnifera
Origin, Historical background, Active principles uses and cultivation practices (including organic farming) of the
following aromatic plants: a) Lemon grass (Cymbopogon flexuosus)
UNIT -II
Distillation of aromatic plants: a) Description of distillation units .b) Principles of distillation .c) Methods of distillation .d)
Maintenance and precautions for distillation units. e) Yields and recoveries of different aromatic plants
UNIT-III
Techniques involved in the isolation in the biomolecules of medicinal importance- solvent extraction, chemical separation,
steam distillation, soxhlet extraction
UNIT –IV
1. Biosynthetic pathways important phytochemicals
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Reference Books-
Pharmacognosy- Kokate et al
Pharmacognosy- Wallis
OR
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Unit II
DNA analysis: Labelling of DNA & RNA probes, southern & florescence in-situ hybridization, DNA
fingerprinting, chromosome walking.
Techniques for gene expression: Northern & western blotting, gelretardation technique, DNA footprinting,
primer extension, S1 mapping,
reporter assays.
Unit III
Proteomics as a tool for plant genetics, breeding & diversity studies.Protein extraction/ purification techniques viz
electrophoresis & column chromatography.Protein sequencing methods, detection of post translational
modifications of proteins, methods of analysis of gene expression at RNA and protein level, large scale expression
such as Microarray based techniques. Protein Engineering and metabolic
Unit IV
RNA interference- Introduction, RNAi as tool for gene expression. RNAias a potential therapy.Use of
transposons in genetic analysis: Transposons & T-DNA tagging & its use in identification & isolation of
genes.Introduction to genome editing with reference to CRISPR/Cas
system.
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Refrence Books-
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
vi. Comparative study of species diversity of different Genera from published Floras (Jaccard index Coefficient). The student
should be provided data on specific Genera represented in therelevant Floras.
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
4. Organoleptic and Microscopic analysis, identification and adulteration check of thefollowing crude drugs.
10. Histochemical identification of the following chemical substances: a) Carbohydrates b) Proteins c)Amino acids
d)Starch e)Tannins f)Enzymes
11. Estimation of oil content in aromatic crops (Clevenger apparatus) and GSC analysis of oil samples for identification of
major compounds.
OR
Unit–IVB-
1. Isolation and purification of genomic DNA from plant materials.
2. Isolation and purification of RNA from plants.
3. Culture of plasmid and maintenance of culture.
4. Isolation of plasmid DNA.
5. Quantitative estimation of genomic DNA and RNA usingspectrophotometer.
6. Agarose gel electrophoresis of genomic DNA and RNA anddetection using gel documentation system.
7. Digestions of DNA by restriction enzymes and size fractionation offragments.Ligation of digested fragments.
8. Primer designing. cDNA formation using reverse transcriptase.
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Course Objectives: The objective of this course is to provide students with hands-on training in
specialized areas of plant sciences
Course Outcomes: After completion of this course the students will acquire the following:
9. Training in experimental design and execution
10. Knowledge on techniques and tools of research
11. Designing of research proposal.
12. Writing of research and review paper
Contents
The topic of the research project will be chosen from among the core compulsorycourses/core
elected courses of that year.
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
th
Semester IV
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Shoot Development: Recent views on organization of shoot Apical Meristem and types of vegetative shoot apex in
Gymnosperms and Angiosperms.
Root Development: Organization of root apex and significance of Quiscent center,Recent experimental studies on
differentiation of tissues.
Leaf: Structure with reference to C3 and C4 plants – Kranz and CAM Syndrome.
UNIT -II
Epidermology:
Structural composition of Epidermal cells, stomata and trichomes b). Epidermal cell complex – Structure, orientation and
arrangement
ultrastructure of guard cells. Ontogeny of Paracyctic, diacytic, and anisocytic stomata.Classification of trichomes.
UNIT -III
c). Morphology and arrangement of Vessels, Axial Parenchyma Fibres and Ray parenchyma and their value in wood
identification.
UNIT – IV
Palynology: Introduction and scope of palynological science,Morphology of pollen – Polarity, symmetry, size and shape,
apertural pattern, exinestratification and ornamentation of pollen wall.
Aeropalynology – principles, dissemination, distribution of aerospora and meteorological factors. Pollen and spore allergy
and clinical treatment. Importance of melittopalynology.
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Reference Books-
Cutter, E.G. Plant Anatomy, Part I and II Edward Arnold; London, 1971 and 1978
Metcalf and Chalk. Anatomy of dicots (2nd Edition) (1983). Clarendon Press, Oxford.
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Reference Books-
53
M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Reference Books-
OR
55
M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Unit -I
Concept and importance of plant diseases
Symptoms of plant diseases caused by fungi, bacterial and viruses , Mode of
infections and development of pathogen in plants ,Enzymes and toxins in plant
diseases
Mechanism of defence in plants: Morphological and biochemical defence mechanism, disease resistance. Variability in
plant pathogens: Types of variations, mechanism of variability.
Effect of environment on development of infectious diseases of plants: epidemiology, plant disease forecasting.
Transmission of plant disease
Methods of study of disease of plant, isolation of pathogens and tests of pathogenecity
Principles and method of plant disease control: regulatory methods, cultural and biological methods,physical means,
chemical methods (fungicides chemotherapy), resistant varieties.
Unit –II
Plant Diseases
Study of important symptoms, causal organism, disease cycle and control of the following disease of cropplants in U.P.
caused by fungi, bacteria, viruses and M.L.O.
a. Rots: Fruit and stem rot of papaya, fruit rot and die-back of chillies, rhizome rot of ginger and red rot of sugarcane.
b. Damping of seedling of crop plants.
c. Downy mildew of bajra, crucifers, pea and cucurbits.
d. Powdery mildew of barley, wheat, pea, apple and cucurbits.
e. Rusts of wheat, Cicer (Gram), barley and linseed.
f. Smuts and Bunts: Covered and loose smuts of barley and wheat; smuts of maize, sorghum and sugarcane; loose
smut of rice and bunt of rice.
g. Wilt of arahar, cotton, gram and sugarcane.
h. Leaf spots, blights and necrosis; leaf spot of crucifers, rice and turmeric; Tikka disease of ground nut, early and late
blight of potato; leaf blight of wheat; blast disease of rice, mango abnormalities.
h. Galls and other abnormalities stem, galls of coriander, ergot of bajra, leaf curl of peach and apple.
UNIT –III
Bacterial Disease
Citrus cankers, blight of cotton, angular leaf spot, black and scattering blight; blight of rice, brown rot of potato, red stripe
of sugarcane, tundu diseases of wheat.
UNIT -IV
Virus Disease
Mosaics of apple, cucurbits, sugarcane, papaya, potato (Potato virus A, X,Y) and tobacco vein mosaic of bhindi, Yellow
mosaic of legumes.
Leaf curl of chillies, papaya, tobacco and tomato; leaf roll of potato bunchy top of banana, tristeza ofcitrus; tug of rice
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Disease cuased by M.L.O.: Little leaf of brinjal, curling of citrus, purple top of potato, sesame phyllody,grassy shoot of
sugarcane.
Refrence Books-
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
UNIT –I
1. Introduction and Scope of Pharmacognosy: Pharmacognosy and modern medicine
2. Crude plant drugs
a) Sources: Geographical, Biological, Cell Culture and Sea
b) Classification: Morphological (Organized and unorganized), Taxonomical, Chemical,
Pharmacological and alphabetical
3. Indigenous tradional drugs and their market adulteration of Punarnava,
Shankhapuspi (Clitoria), Indian goose-berry, Tulasi, Commiphora, Kalmegh
UNIT -II
4. Types of Plant drug and their Pharmacognostic study
a) Root drugs; Glycyrrhiza and Ipecac, Raulvolfia, Satavari, Colcus,
Withania
b) Rhizome drugs, Ginger
c) Leaf drugs, Andrographis, Clitoria, Senna
d) Bark drugs: Terminalia arjuna, Holorrhena
e) Flower drugs: Saffron
f) Seed drugs: Piper longum, Mucuna
g) Fruit drugs: Cumin, Amla, Senna pods
h) Whole plant drugs: Catheranthus roseus
UNIT -III
5.Evaluation of the drugs; Organoleptic, Microscopic, Physical Chemical
and Biological methods of evaluation
6. A brief account of various drug constituents: Carbohydrates, Cardiac glycosides, alkaloids,
volatile oils, resins quinines and steroids with particular reference to
Accacia gum, amla, Coleus, Satavari, Rauvolfia
UNIT -IV
7. Medicinal Principles and powder analysis of Curcuma, Cloves, Senna, Fennel and
Cinnamon
8. Large scale Industrial preparation of Crude Drugs
a) Types of reactors used and extraction methods
b) Active principles and non-active principle of drugs
c) Import and Export potentials of Crude Drugs
d) Preparation of crude drugs in indigenous system of medicine
e) Quality control test – contamination, Adulteration
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Reference Books-
OR
59
M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Unit -I
Principles and concept of plant breeding; techniques of plant breeding, selection, hybridization, acclimatization, heterosis,
sterility and incompatibility
Unit –II
chimera and graft hybrids, breeding for disease, insect and drought resistance, crop improvement and methods of breeding
of wheat maize, paddy, sugarcane, arhar, potato and cotton with special reference to work done in India
UNIT –III
In-vitro techniques in relation to plant breeding classification, meristem culture, anther and pollen culture, tissue and cell
culture, cybrids, protoplast fusion, hybridomas achievements and prospects
UNIT -IV
Biometry and experimental designs : Importance of biometry in plant breeding data representation, classification tabulation,
frequency, Null hypothesis, chi-square test (Numericals in relation to the genetics and plant breeding) correlation and
regression in relation to plant breeding) Experimental designs
60
M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Refrence Books-
4. Genetics by Strickberger
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
1.Study of the following locally available taxa (living sand herbarium) belonging to:
a) Cleome b) Cassia c) Sida d) Indigofera e) Euphorbia f) Corchorus g)Tephrosia
h) Phyllanthus i)Brachiaria j)Ipomoea
1. Identification of key characters of species of above genera and construction of keys
2. Study of the seed morphology of the following:
a) Cleome b) Gossypium c ) Calotropis d) Annona e) Cyperus f) Oryza g) Castor/
Croton h) Portulaca i) Tecoma j) Glinus / Mullugo k)Pulses
3. Identification of selected families based on their Androecium and
Gynoeciumgiven in the mixture
a) Malvaceae b) Meliaceae c) Fabaceae d) Umbelliferae e) Cucurbiataceae
f) Compositsae g) Euphorbaceae h) Lamiaceae\
4. Students are required to maintain field note book and record of the taxa
occurring in the areas visited
5. Students are required to prepare herbarium of plants collected during field trips
6. Record.
OR
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M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
OR
64
M.Sc. Botany Syllabus VBS Purvanchal University, Jaunpur
Course Objectives: The objective of this course is to provide students with hands-on training in
specialized areas of plant sciences
Course Outcomes: After completion of this course the students will acquire the following:
13. Training in experimental design and execution
14. Knowledge on techniques and tools of research
15. Designing of research proposal.
16. Writing of research and review paper
Contents
The topic of the research project will be chosen from among the core compulsorycourses/core
elected courses of that year.
65