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Seat matrix

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5 views3 pages

n5c41665cd4cbb

Seat matrix

Uploaded by

nagarmanoj132
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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FUNGAL CULTURE MEDIA

LEARNING OBJECTIVE

 Concept of culture media


 Preparation of culture media
 Specific use of culture media.

1. INTRODUCTION

Culture media: It is the media that allows physical growth of the microorganisms by
providing suitable pH, osmotic pressure and nutrition.

Fungal culture media: They are divided into two groups.

Non selective culture media: They will allow the growth of all clinically important fungi and
does not contain antibiotics. Ex: SDA

Selective culture media: This medium is used to isolate specific clinically important fungi of
interest. It may contain some antibiotics such to prevent bacterial infection. Ex: Yeast extract
phosphate agar.

1.1 SABORAUD DEXTROSE AGAR WITHOUT ANTIBIOTICS

This media is used to study fungal structure.

Composition:

 Peptone 10gm
 4% glucose 40gm
 Agar 15gm
 Distilled Water 1000ml

Procedure:

 Mix all the contents in a conical flask and boil it.


 Adjust pH to 5.6
 Dispense in tubes and autoclave for 10 minutes at 15psi.

1.2 SABORAUD DEXTROSE AGAR WITH ANTIBIOTICS

Composition:

 Peptone 10gm
 4%glucose 40gm
 Chloramphenicol 0.5gm
o Acetone 10mll
o Cycloheximide 0.5gm
 95% ethanol 10 ml
1
 Agar 15gm
 Distilled water 1000ml

PROCEDURE

 Dissolve all the contents in a conical flask.


 Mix all the contents in a conical flask and boil this mixture.
 Cool this mixture to about 50 degree.
 Adjust pH to 6.5.
 Dispense in tubes and autoclave for 10mins at 15psi.

1.3 CORN MEAL AGAR


COMPOSITION

 Yellow corn meal 40gm


 Agar 15gm
 Distilled water 1000ml

PROCEDURE

Place corn meal in a conical flask and dissolve in 900ml of distilled water. Boil this mixture

 Cool it to 50C and filter.


 Make the volume to 1000ml
 Now add agar by gently heating and agitation.
 Pour the medium into tubes.
 Autoclave for 15 minutes at 15 psi.

1.4 BRAIN HEART INFUSION


This is an enriched and not used for skin, hair and nail specimens.

COMPOSITION

 Calf brain infusion 200gm


 Beef brain infusion 250gm
 Peptone 10gm
 Agar 15gm
 Glucose 02gm
 Sodium chloride 05gm
 Disodium phosphate 2.5gm
 Distilled water 1000ml

PROCEDURE

 Mix all the contents in a conical flask and boil this mixture.

2
 Adjust the pH to 7.4.
 Pour this medium into tubes.
 Autoclave for 15 minutes at 15psi.

VERY SHORT ANSWER TYPES QUESTIONS

Q1 Define culture media.

Q2 .SDA, CMA BHI?

Q3. Name the antibiotics used in SDA.

Q4.Give examples of selective culture media.

Q5. Give examples of culture media.

SHORT ANSWER TYPES QUESTIONS

Q1.Discuss the characteristics of culture media.

Q2. Discuss the method of preparation of culture media.

Q3.Discuss the preparation of CMA.

Q4. Discuss the method of preparation of BHI.

Q5.Discuss method of preparation of SDA.

LONG ANSWER TYPES QUESTIONS

Q1.Discuss the composition and preparation of SDA with and without antibiotics.

Q2. Discuss the method of preparation of SDA, CMI and BHI.

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