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Training Manual AI Part 2 06-16-2023 12-50-56
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Training Manual AI Part 2 06-16-2023 12-50-56
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“Training Manel on Ovioe Artifical insemination (er 11: Pregnancy Diagnosis of Sheep by Ultrasonography iagnosisof pregnancy and determination of fetal numbers in management ofthe ewes in of lambs. In adstion, the adequate period |. weaning weight and survivabil ion would be useful to dey offlactating femal aceurate information on and tomonitorthe females neater, Ultrasonography utilizes pulses of sound waves that are projected towards its intended structures and within seconds the echoes are returned to the source from 3 they originate. Various body tissues conduct sound differently. Somme sues absorb sound waves while oters reflect thom. The density of su dictates the speed at which the echoes ret. The transducer fot pregnancy at 40-50 days of gestation with 95 ~ 100% securacy, A portable convex ara ‘used for ultrasonography of sheep at die ‘90 days Following measurements options are available inthe S-600 ultrasound machine OB Mode: ex pos ts posible ve displays two reduced - size images on the thers orenor bh mapes canbe oe. @ Laces Training Manwal on Ovae Artie sem (i) BA and MModes: sada particularly nd MMede nce ca be inBM Mode, -Modoare adjusted 1 heart rate meas rementeanbe made. Sweep sp BM] key onthe keyboard eee ‘0 calculate and measire the variables inthe sume Up to completion of two or more measurement valve sichasarato or anengleof Tomeasure distances, dothe following: Press [MEASURE] function key to display B-Mode measure menu. Th Dighlighte,Pressthe [SELECT] key A cursor appears onthe sanning ar ‘cursor to one end of the distance measurement. Press yum release the frst point and me UMEASURE] key. A dot ‘pears. Measure second stance as same a first. When yo ‘eright side ofthe sereen, Press [Ro sect Heartrate measurements: 8ISBLEC’ the distance measurement. Press the [SELECT] key. Ifyou net [SELECT] key againtoreleate heist Tine, press the [MEASURE] key. A dotted lines right sie ofthe screen (Ttime, H: heart rate). Repeat step raining Manual on Ovin Artsical insemination lide point to move the cursor to one end of cy. Acutsor appears on the scanning area Usethe 3-6 fr another measurement heart rate measurements are displayed on the right sie ofthe screen Precaution during ultrasonography: 0 «i iy For getting better image, cleanliness of probeisnecesar. Daring the scanning of sheep wit sector array probe the operator shoul si onitsleftsideand beside its back legs ‘The probe is placed in the groin areas, between the udder and the right properly shaved. ‘Approved scanning gel should be applied on the shaved are a ss well a8 on the probe to ensure 1 be gradually tured in clockwise direction without moving it from the original Ia 1s of pregnancy, when the wera has become very larg, or where ther ae multiple ferses, the probe should be moved on theabdomen or adistance of approximately 100mm as ‘Tining Manual on Ovine Artie Chapter 12: Disease Transmision through at She in Sheep 7 % Perspective of disease transmissic ae =» on cay many pe ee ing vn esc cb chology insheep eeinprrenene tee cams ee uti mj joni deo se a th poly of nfectons aget eg anil ems on faemenincotnec Alpen on trohemen 0 pet of esting ene On ‘organisms, which are present in Ee bd qeaiehres at tn te crs tated prions ola apo As of micro-organisms are added in the semen during collection, proce eee 7 Dicroorganisme are Escherichia Cone setback with A. is that semen placed i he vers isnot exposed tthe known bacteria eet ofthe Vaginal and cerviea seereion during orsus. Diseases transmis by semen in shep re meatoned presence and transmission stansfinfctiveagentsin or though sme, andiransmited otand mouth disease Bluetongue Campylobactrioss John's diseasesexton Ove iil ine stratedinsemenandproveby ransmited B_Infectiveagent Rinderpest Rittvalley few itsraminans(PPR) Enzootcaborion(Clamysiosis) C_Infectiveagentdemonsiratedinsemen butnottansmited = re jtransmitted 1 ttn ei mi = vali nase of infection or in a nonclinical carrier st = ee eat tion is also high because freezing can maintain inf eer reduction and contingency planning , eects are teven! precedes tnt ca be pred to reduce the ks scinted wi ingoduction or spread ofitess, maximise the detention nd detection of cisezed animals nd thedisease strategy contol programs. These ety props level tht permis the intrational distribution animals or humans with specific pathos" ‘organisms thatcanbetansmitedby semen Siek animals should be placed in immedi isolation and subjected to detailed vee™? © c ¥ + Seoptivenm nkteaedestty ay palomitas Sn 2 esmenolncldfanasaia ect eu + Enetnone sets led peda The eaten etoangersoe : sna nn yc sen Any end wg ean ses hog mast ‘render the product safe, eae «Alsen {Tec pee me ie qaera stn Ae site ft cen nie sci be enon eases released for use. se + Hetantihdeom darned gs dine te ieeuetteneemon Fareonalone nsec ee Pen 2 ec omsmaybemace foms recovered animal donor andthe day of production, onrledand staat the centre shouldbe technically hygiene opel Provide. + Staffshouldbe encouraged i animals residing ofthe cence, epily thse of the same or similar species. Whee tis snc posible, suitable hygienic procedures should be maintained, * Inseminators and veterinarians shouldbe restricted fom aces ot ont withthe anima ules ‘hey change nt clean protective lig nd footwear provided the quarantine ety points Training off: SisTeitoy couse every yea or coursesshouldeover: ved Early recognition of signs ofdissse; Emergency strategies for control Role and responsitiltesof staff and Significance of anoutbreaktothe operation ofthe AB ngs) ® : 1raining Maal on vine ria insemination ali sting, presenta great risk tthe ced on farms fom avimals tht ave bai oath esting, reset rel Hk tthe of infection. To reduce the risks ascociatod with this eommom 230d Donor sires are esthestedand cenifd fee of isase forthe 30 ys on nen ‘Semen collected from these donors should oaly be transferred otber anima ofthe donors; and Javon rom icensedsemen. ease through unlicensed semen, Inthe event pious material and ‘of an emergency disease outbreak any fedtohave been contaminated would be destoye. Record kecping: Concise, aceurate and accessible records, econ —— nam aceefmeneeny dose oubreak ee imal product or people, thereby Timiting possible contamination and to preventbs unnecessary deemed infected or highly suspect Such records mustincloée the following desis Animals: + Breed,sexandage tat, tag, brands, photo of removal method of ans ‘Animal contacts oo, tag, brands, photo of donor numbers, date, destination, all straws tobe identified by tof collection andcenreofeallection . v es SEOs Mant on Orne Arial semtstion Chapter 13: i Tater 13: Factors infuencing success of AI in sheep contin fr Ala fot an When dipping toate aeanee rats after Aare ge te Al echology tel iil that afer unlikely ob tout te heathy, are in good body condition and ae fie of urbe offers, which may influence the 1 qNumbe of spermatato and ste of epson: Asa generals peat ae eid {orwerinethan for cervical ine es focal thn for vaginal insemination Table?) Table 2: Minimum numberof motile sermon st iferent sites in sheep (0 ia milion) | ‘Type ofsemen Vagina env ‘Utes (laparoscopy) Fresh 300 100) 20 Liquid stored Notefetve 150 20 Frosen-thawed Notetetve 180 20 ble, the amber of Spemmtozoa can be increased, as there no har, In of insemination sth ters andthe number of spematzos is increased oat ‘Survival of spermatozoa: The semen of tam is seastve to environmental and other changes and, therefore, strict care shouldbe taken to avoid damage when banding semen, Survival of spermatozoa is isan se woennne ing met ol feofthesemen. harmful to sermen, Short exposure may reduce viability and 230-40 syns Alo aoid exposure of erento stong Mhoesent igh or ° graining Manual on vine Artist nsemintih (aly Metts; Cones with metal of ey ni harmful to speratozs. Only gas or net syste ‘material equipments shouldbe used forcllectio,cilaion storageand inser (Go) Water: Water educesthe osmotic pressure ofthe sein plata nd tery ‘equipments shouldbe thoroughly dried © Smoke: Te gases: arf fr semen, No bush Fir the semen laboratory collection and insemination wea. (0) Impurities and bacteria: Boeri, dt, bi, urine and ater comaiants reves the Viabty or Tellthespenmatoze, Aways over semen wth akuminium fiend clean he prepuce oframs before collection, Add antibiotistothedluent to cont betes (iy Disinectants and andzepes: Disifectans and ansetcs are harmfil (© spemmatozon, “Thoroughly dry the diinfctedequipment beforewse. “Long exposure to ars Semen shouldbe wed fr insemination or storage the caries after wn Long exposure to oxygen inthe ar nereases metabolic activity of spermatozoa and spermatozoa, All orsmoking should beallowed near below 7.0 (ave) reduces um pH of media surrounding the spacty of dient Shits ia pH either sbove ‘of spermatozoa, The buffer should man spermatozoa. 6) Osmotic pressure of dient: Spermatozoa rein wot longest when suspended in an isotonic “ituent Hypertons (higher solute conten than spertozoa) media and hypotonic media (lower coverage and results, heat shou females shouldbe inseminated es the next momingandi ‘When estrus hs been synchronized theres no need o detec ert; insemination ean be done a snimals.L progestogen progesterone spongesare sen conjunction with PMSG, the majority afte ee Papen about 60 hours afer sponge withdrawal. In such cass th ‘optima time of cervical insemination is between 48.58 hours (generally $5 hours) afer SPORE? © . ¥ here ozen-shawed me amsemen is generally sponge witha. aie seer ace foment sme Onanmist.nargureny aoe a carn ana ine teres a recommended when IUALs performed mis used Prsenaney wien the placentas being stl 11. Hygiene conditions: The inseminaoro operat ious agents from one ee othe oer fom et asl al epupat ned an rou was sb boos and cles afer ch insemination session. The use of diposbesis sen resorinened. © 8 ¥“rating Manual on ving Ae esemination BKSeSEEZLBESEEE_E SS ——— Chapter 14: Data management at te eer Lambing percentage= (No.ofeweslambed/n0.ofewes inseminated) 100, ‘Thefollowinginformation should benscesarytomantain proper records Name ofthe lockowner [Nameof he village Date of insemination Sirebreed Flockno. ‘Stage oflambing Heat (stage) stinsemination ‘Oasecofheat (interval) ‘Lambing date ith weightoflamb S-month weightoflam fav) 6-momh weightoflam (vi) 9-month weighto Yearling weightoflanb Chapter 1S: Import-export regulations of ovine germ plasm In Indi, there ae several Acs and pol iis enacted sae practices. Among those Acts, Inporsend Espa and Regulation) Act, 1992 and impor: Expo ‘The Planning Commission's report on Ani lan (2007-12) has noted the poor genetic potent ‘concemed, access 10 sheep genetics can improve the efficiency breeds can be imported and evalustedalong side dome ian sheep industy ‘Current GOI regulations restrict import of and embryos. The Livestock Importation Act, ‘roducs, Livestock Importation Ac, 1898 9 (Amendmet ibreedstose been made to regulate of vetock products in soch manne these imports do not adversely affect the human and animal heath population oft ‘he powers of the Cental Goverament under the amended Livestock inporion A (tAnimal Husbandry and Dairying has issued a notifeation dated 7th Jay 2 livestok products, the Notification the Central Government rests the imprint Indi fal ivesock ing + Meat and products of all kinds of including tes, chilled and fozen sheep, goat, Eezandegg powder, Mitkand mitk products; Bovine, ovine and caprine embryos, ova orsemen; and Pet food products of animal erin; ‘The import ofthese product shall be allowed o ® = ¥‘Training Manual on Ovine Artifical Insemination eS Ou
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