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2019 DNA Extraction Experiment

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6 views

2019 DNA Extraction Experiment

Uploaded by

akilan.akil8838
Copyright
© © All Rights Reserved
Available Formats
Download as PDF, TXT or read online on Scribd
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Class 9-____ Name ＿＿＿＿＿＿＿＿＿＿＿＿＿＿

Science English Experiment: Extraction of Banana DNA

Introduction:
All living things are made up of cells.
Each cell has a nucleus which controls the life of the cell.
Each nucleus has chromosomes which contain DNA.
DNA dissolves in salt water and precipitates in alcohol.

Purpose:
To extract DNA from banana cells by using items easily available in the
kitchen.

Materials:
Banana Liquid detergent Cooled ethanol
Mortar and pestle Glass rod Beaker
Tea bag Salt Tap water
Wooden chopsticks Test tube

Procedure:
1. Preparation of Solution:
In a beaker mix 8g of salt with 3mL of liquid detergent.
Fill the beaker with water to a volume of 50mL and stir gently with the
glass rod.

2. Preparation of Sample:
Mash-up a 5cm piece of banana and place it in the beaker.

3. Extraction of DNA:
Gently stir mashed banana suspension then let it stand for 10 minutes.
(The liquid detergent is a surface acting agent [ called a surfactant ] and
helps the DNA dissolve into salt water.)

4. Filtration:
Use a tea bag to filter the mashed banana suspension into a smaller
beaker, then pour the resulting filtrate into a test tube. Fill the test tube
about halfway.

5. Precipitation of DNA:
Add an equal amount of ethanol to the filtrate by slowly pouring it down a
glass rod. (Ethanol is less dense than water, so it sits on the water solution
making 2 different layers.)
Wait for a white precipitate to form on the boundary phase. (DNA does not
dissolve in ethanol. It will precipitate out.)

6. Collection of DNA:
Gently stir the solution with a wooden chopstick. The fibers will stick to it
and the DNA can be spooled and collected.

Write down what you see! Use lots of details. (group question)

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