* 0019655390501 *

,

 

,

Cambridge IGCSE™

¬Wz> 3mEuO]~kyEW
¬8:aL¨Q3b†tªm««ª‡‚
¥¥¥•u•¥•u¥ U5ee uU
* 6 3 2 2 1 1 7 2 8 9 *

BIOLOGY 0610/52
Paper 5 Practical Test May/June 2024

1 hour 15 minutes

You must answer on the question paper.

You will need: The materials and apparatus listed in the confidential instructions

INSTRUCTIONS
● Answer all questions.
● Use a black or dark blue pen. You may use an HB pencil for any diagrams or graphs.
● Write your name, centre number and candidate number in the boxes at the top of the page.
● Write your answer to each question in the space provided.
● Do not use an erasable pen or correction fluid.
● Do not write on any bar codes.
● You may use a calculator.
● You should show all your working and use appropriate units.

INFORMATION
● The total mark for this paper is 40.
● The number of marks for each question or part question is shown in brackets [ ].

For Examiner’s Use

Total

This document has 12 pages. Any blank pages are indicated.

DC (PB/CB) 330272/3
© UCLES 2024 [Turn over
 * 0019655390502 *

DO NOT WRITE IN THIS MARGIN

2
,

 

,

1 You are going to investigate the effect of acid concentration on the rate of diffusion.

Bromothymol blue is an indicator that turns yellow in the presence of an acid.

Read all the instructions but DO NOT DO THEM until you have drawn a table for your results
in the space provided in 1(a)(iii).

You should use the safety equipment provided while you are doing the practical work.

Step 1 Label three beakers A1, A2 and A3.

DO NOT WRITE IN THIS MARGIN

Step 2 Add 20 cm3 of 1 mol per dm3 hydrochloric acid solution to beaker A1.

Step 3 Add 2 cm3 of 1 mol per dm3 hydrochloric acid solution to beaker A2.

Step 4 Add 18 cm3 of distilled water to the hydrochloric acid solution in beaker A2 and mix well.

Step 5 Use a clean syringe to transfer 2 cm3 of the solution from beaker A2 to beaker A3.

Step 6 Add 18 cm3 of distilled water to beaker A3 and mix well.

Table 1.1 shows the final concentrations of the hydrochloric acid solution in each beaker.

DO NOT WRITE IN THIS MARGIN

Table 1.1

final concentration of the final volume of the

beaker hydrochloric acid solution solution / cm3
/ mol per dm3

A1 1.00 20

A2 0.10
………

DO NOT WRITE IN THIS MARGIN

A3 0.01 20

(a) (i) Complete Table 1.1 by calculating the final volume of the solution in beaker A2.

Write your answer in Table 1.1. [1]

You are provided with three test‑tubes containing bromothymol blue indicator in agar jelly.

Step 7 Label the three test‑tubes A1, A2 and A3.

Step 8 Use a clean syringe to transfer 5 cm3 of the hydrochloric acid solution from beaker A1 to
DO NOT WRITE IN THIS MARGIN

test‑tube A1.

Step 9 Use a clean syringe to transfer 5 cm3 of the hydrochloric acid solution from beaker A2 to
test‑tube A2.

Step 10 Use a clean syringe to transfer 5 cm3 of the hydrochloric acid solution from beaker A3 to
test‑tube A3.
ĬÕú¾Ġ³íÅõÏāÝþêûÇĎ×
© UCLES 2024 Ĭ¸¼âÉĤÕÕÙûĆąÅđÛÒÿĂ
ĥµõĕµõåõÅåÕĕµĥÅÅÕÕ
0610/52/M/J/24
 * 0019655390503 *
DO NOT WRITE IN THIS MARGIN

3
,

 

,

Step 11 Raise your hand when you are ready for warm water to be poured into the beaker
labelled water‑bath.

Step 12 Place the three test‑tubes into the warm water‑bath and start the stop‑clock.

Leave the test‑tubes in the water‑bath for 20 minutes.

While you are waiting continue with the other questions.

Step 13 After 20 minutes, remove the test‑tubes from the water‑bath and place them in the
test‑tube rack.
DO NOT WRITE IN THIS MARGIN

(ii) Record the temperature of the water in the water‑bath.

temperature ........................................................... °C [1]

Step 14 Remove the hydrochloric acid solution from each test‑tube by pouring the solution in
each test‑tube into the beaker labelled waste.

Step 15 For each test‑tube, measure the distance between the top of the agar and the point at
which the blue agar has become yellow. Fig. 1.1 shows where to take the measurement.

Record your measurements in your table in 1(a)(iii).

DO NOT WRITE IN THIS MARGIN

top of the agar

point at which the

blue agar has distance to measure
become yellow
DO NOT WRITE IN THIS MARGIN

Fig. 1.1
DO NOT WRITE IN THIS MARGIN

Ĭ×ú¾Ġ³íÅõÏāÝþêùÇĎ×
© UCLES 2024 Ĭ¸»áÁĦÙåàýûÄđĩÿÒïĂ
ĥµąÕõĕÅĕÕÕąĕµąåąÅÕ
0610/52/M/J/24 [Turn over
 * 0019655390504 *

DO NOT WRITE IN THIS MARGIN

4
,

 

,

(iii) Prepare a table and record your results in the space provided.

Include the final concentration of hydrochloric acid in your table.

DO NOT WRITE IN THIS MARGIN

DO NOT WRITE IN THIS MARGIN
[4]

(iv) State a conclusion for your results.

...........................................................................................................................................

...........................................................................................................................................

DO NOT WRITE IN THIS MARGIN

..................................................................................................................................... [1]

(v) Suggest why the temperature of the water‑bath did not need to be maintained during
the investigation.

...........................................................................................................................................

...........................................................................................................................................

..................................................................................................................................... [1]
DO NOT WRITE IN THIS MARGIN

ĬÕú¾Ġ³íÅõÏāÝþìûÇĐ×
© UCLES 2024 Ĭ¸»äÁĠëäÛăô˳ÅÝÂćĂ
ĥąÕÕµĕŵµõõĕõąąąÕÕ
0610/52/M/J/24
 * 0019655390505 *
DO NOT WRITE IN THIS MARGIN

5
,

 

,

(vi) Suggest why the hydrochloric acid was removed from the test‑tube in step 14 before
measuring the distance of the yellow colour.

...........................................................................................................................................

...........................................................................................................................................

..................................................................................................................................... [1]

(vii) Identify the independent variable and the dependent variable in this investigation.
DO NOT WRITE IN THIS MARGIN

independent variable .........................................................................................................

...........................................................................................................................................

dependent variable ............................................................................................................

...........................................................................................................................................
[2]

(viii) Explain why suitable eye protection should be worn when doing this investigation.

………………… ...........................................................................................................……
DO NOT WRITE IN THIS MARGIN

...........................................................................................................................................

..................................................................................................................................... [1]
DO NOT WRITE IN THIS MARGIN
DO NOT WRITE IN THIS MARGIN

Ĭ×ú¾Ġ³íÅõÏāÝþìùÇĐ×
© UCLES 2024 Ĭ¸¼ãÉĪçÔÞõýþħ­ùÂ÷Ă
ĥąåĕõõåÕåąåĕõĥĥÅÅÕ
0610/52/M/J/24 [Turn over
 * 0019655390506 *

DO NOT WRITE IN THIS MARGIN

6
,

 

,

(b) In an experiment, a student cut different‑sized cubes of agar containing bromothymol blue
indicator. The student placed the cubes in hydrochloric acid and measured the time taken for
each cube to become yellow.

The results are shown in Table 1.2.

Table 1.2

length of the volume of time taken for the cubes

cube sides / mm the cube / mm3 to become yellow / s

DO NOT WRITE IN THIS MARGIN

2 8 35

4 60
...........

6 216 170

8 512 275

DO NOT WRITE IN THIS MARGIN

10 1000 445

(i) Complete Table 1.2 by calculating the volume of the 4 mm cube.

Write your answer in Table 1.2. [1]

DO NOT WRITE IN THIS MARGIN

DO NOT WRITE IN THIS MARGIN

ĬÙú¾Ġ³íÅõÏāÝþéùÆĎ×
© UCLES 2024 Ĭ¸»ãÆĤ¿Úæòð÷ćÅĜĪćĂ
ĥÕąĕõÕåõĥĥąĕµĥÅąĕÕ
0610/52/M/J/24
 * 0019655390607 *
DO NOT WRITE IN THIS MARGIN

7
,

 

,

(ii) Plot a line graph on the grid of the length of the cube sides against the time taken for the
cubes to become yellow.
DO NOT WRITE IN THIS MARGIN
DO NOT WRITE IN THIS MARGIN

[4]

(iii) Use your graph to estimate the time taken for a cube with a side length of 3 mm to
become yellow.

Show on your graph how you obtained your answer.

.............................................................. s
DO NOT WRITE IN THIS MARGIN

[2]

(iv) State two variables that should have been kept constant in this investigation.

1 ........................................................................................................................................

...........................................................................................................................................

2 ........................................................................................................................................

...........................................................................................................................................
[2]
DO NOT WRITE IN THIS MARGIN

[Total: 21]

ĬÛù¿Ġ³íÅõÏāÝþêùÆĎ×
© UCLES 2024 Ĭ¸»áÉğ°°ÕóĄÄ°ąĜÚïĂ
ĥĥåÕõõąĕąĥåĕõåÅÅåÕ
0610/52/M/J/24 [Turn over
 * 0019655390608 *

DO NOT WRITE IN THIS MARGIN

8
,

 

,

2 (a) When a seed germinates it grows into a seedling.

Fig. 2.1 is a photograph of a seedling.

A B

DO NOT WRITE IN THIS MARGIN

Fig. 2.1

DO NOT WRITE IN THIS MARGIN

(i) Make a large drawing of the seedling shown in Fig. 2.1.

DO NOT WRITE IN THIS MARGIN

DO NOT WRITE IN THIS MARGIN

[4]
ĬÙù¿Ġ³íÅõÏāÝþìûÆĐ×
© UCLES 2024 Ĭ¸»äÉĥ¾¹âíċËĎéºÊćĂ
ĥÕõÕµõąµĥÅÕĕµåĥŵÕ
0610/52/M/J/24
 * 0019655390609 *
DO NOT WRITE IN THIS MARGIN

9
,

 

,

(ii) Line AB represents the width of the seedling.

Measure the length of line AB on Fig. 2.1.

length of line AB ........................................................ mm

The actual width of the seedling is 9 mm.

Calculate the magnification of the seedling in Fig. 2.1 using the formula and your
measurement.
DO NOT WRITE IN THIS MARGIN

length of line AB in Fig. 2.1

magnification =
actual width of the seedling

Give your answer to two significant figures.

Space for working.

DO NOT WRITE IN THIS MARGIN

................................................................
[3]
DO NOT WRITE IN THIS MARGIN
DO NOT WRITE IN THIS MARGIN

ĬÛù¿Ġ³íÅõÏāÝþìùÆĐ×
© UCLES 2024 Ĭ¸¼ãÁģÂÉ×ċöþÊÑĞÊ÷Ă
ĥÕąĕõĕĥÕõµąĕµÅąąåÕ
0610/52/M/J/24 [Turn over
 * 0019655390610 *

DO NOT WRITE IN THIS MARGIN

10
,

 

,

(iii) Fig. 2.2 shows photographs of two different seedlings, P and Q.

DO NOT WRITE IN THIS MARGIN

P Q

Fig. 2.2

DO NOT WRITE IN THIS MARGIN

Describe one visible similarity and two visible differences between seedling P and
seedling Q in Fig. 2.2.

similarity 1 ..........................................................................................................................

...........................................................................................................................................

difference 1 ...........................................................................................................………..

...........................................................................................................................................

difference 2 ........................................................................................................................

DO NOT WRITE IN THIS MARGIN

...........................................................................................................................................
[3]

(b) As seedlings grow, they change starch into reducing sugars.

Describe the methods you would use to test a sample of seedlings for starch and
reducing sugars.

starch test .................................................................................................................................

...................................................................................................................................................

...................................................................................................................................................
DO NOT WRITE IN THIS MARGIN

reducing sugar test ............................................................................................................... …

...................................................................................................................................................

...................................................................................................................................................
[3]
ĬÙù¿Ġ³íÅõÏāÝþéûÈĎ×
© UCLES 2024 Ĭ¸ºäÄħÆßàöĄġòëÎòïĂ
ĥąĕĕµõåµÅõÕÕµąĥÅąÕ
0610/52/M/J/24
 * 0019655390611 *
DO NOT WRITE IN THIS MARGIN

11
,

 
,

(c) When light shines from one side, seedlings grow towards the light.

Fig. 2.3 shows a diagram of a seedling growing towards a light source. The angle of growth is
shown on the diagram.

light source
DO NOT WRITE IN THIS MARGIN

Fig. 2.3

Plan an investigation to find out how light intensity affects the angle at which the
DO NOT WRITE IN THIS MARGIN

seedling grows.

...................................................................................................................................................

...................................................................................................................................................

...................................................................................................................................................

...................................................................................................................................................

...................................................................................................................................................

...................................................................................................................................................
DO NOT WRITE IN THIS MARGIN

...................................................................................................................................................

...................................................................................................................................................

...................................................................................................................................................

...................................................................................................................................................

...................................................................................................................................................

...................................................................................................................................................
DO NOT WRITE IN THIS MARGIN

............................................................................................................................................. [6]

[Total: 19]

ĬÛù¿Ġ³íÅõÏāÝþéùÈĎ×
© UCLES 2024 Ĭ¸¹ãÌġÊÏÙĄíèæÓĊòÿĂ
ĥąĥÕõĕÅÕÕąąÕµĥąąĕÕ
0610/52/M/J/24
 * 0019655390612 *

DO NOT WRITE IN THIS MARGIN

12
,

 
,

BLANK PAGE

DO NOT WRITE IN THIS MARGIN

DO NOT WRITE IN THIS MARGIN
DO NOT WRITE IN THIS MARGIN
DO NOT WRITE IN THIS MARGIN

Permission to reproduce items where third‑party owned material protected by copyright is included has been sought and cleared where possible. Every
reasonable effort has been made by the publisher (UCLES) to trace copyright holders, but if any items requiring clearance have unwittingly been included, the
publisher will be pleased to make amends at the earliest possible opportunity.

To avoid the issue of disclosure of answer‑related information to candidates, all copyright acknowledgements are reproduced online in the Cambridge
Assessment International Education Copyright Acknowledgements Booklet. This is produced for each series of examinations and is freely available to download
at www.cambridgeinternational.org after the live examination series.

Cambridge Assessment International Education is part of Cambridge Assessment. Cambridge Assessment is the brand name of the University of Cambridge
Local Examinations Syndicate (UCLES), which is a department of the University of Cambridge.
ĬÙù¿Ġ³íÅõÏāÝþëûÈĐ×
© UCLES 2024 Ĭ¸¹âÌī¼ÚÞþößĈïìĢ÷Ă
ĥµµÕµĕÅõµåõÕõĥåąąÕ
0610/52/M/J/24