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BIOLOGY 0610/52
Paper 5 Practical Test May/June 2024
1 hour 15 minutes
You will need: The materials and apparatus listed in the confidential instructions
INSTRUCTIONS
● Answer all questions.
● Use a black or dark blue pen. You may use an HB pencil for any diagrams or graphs.
● Write your name, centre number and candidate number in the boxes at the top of the page.
● Write your answer to each question in the space provided.
● Do not use an erasable pen or correction fluid.
● Do not write on any bar codes.
● You may use a calculator.
● You should show all your working and use appropriate units.
INFORMATION
● The total mark for this paper is 40.
● The number of marks for each question or part question is shown in brackets [ ].
Total
DC (PB/CB) 330272/3
© UCLES 2024 [Turn over
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1 You are going to investigate the effect of acid concentration on the rate of diffusion.
Read all the instructions but DO NOT DO THEM until you have drawn a table for your results
in the space provided in 1(a)(iii).
You should use the safety equipment provided while you are doing the practical work.
Step 3 Add 2 cm3 of 1 mol per dm3 hydrochloric acid solution to beaker A2.
Step 4 Add 18 cm3 of distilled water to the hydrochloric acid solution in beaker A2 and mix well.
Step 5 Use a clean syringe to transfer 2 cm3 of the solution from beaker A2 to beaker A3.
Table 1.1 shows the final concentrations of the hydrochloric acid solution in each beaker.
A1 1.00 20
A2 0.10
………
(a) (i) Complete Table 1.1 by calculating the final volume of the solution in beaker A2.
You are provided with three test‑tubes containing bromothymol blue indicator in agar jelly.
Step 8 Use a clean syringe to transfer 5 cm3 of the hydrochloric acid solution from beaker A1 to
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test‑tube A1.
Step 9 Use a clean syringe to transfer 5 cm3 of the hydrochloric acid solution from beaker A2 to
test‑tube A2.
Step 10 Use a clean syringe to transfer 5 cm3 of the hydrochloric acid solution from beaker A3 to
test‑tube A3.
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3
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Step 11 Raise your hand when you are ready for warm water to be poured into the beaker
labelled water‑bath.
Step 12 Place the three test‑tubes into the warm water‑bath and start the stop‑clock.
Step 13 After 20 minutes, remove the test‑tubes from the water‑bath and place them in the
test‑tube rack.
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Step 14 Remove the hydrochloric acid solution from each test‑tube by pouring the solution in
each test‑tube into the beaker labelled waste.
Step 15 For each test‑tube, measure the distance between the top of the agar and the point at
which the blue agar has become yellow. Fig. 1.1 shows where to take the measurement.
Fig. 1.1
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(iii) Prepare a table and record your results in the space provided.
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(v) Suggest why the temperature of the water‑bath did not need to be maintained during
the investigation.
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5
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(vi) Suggest why the hydrochloric acid was removed from the test‑tube in step 14 before
measuring the distance of the yellow colour.
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(vii) Identify the independent variable and the dependent variable in this investigation.
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[2]
(viii) Explain why suitable eye protection should be worn when doing this investigation.
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(b) In an experiment, a student cut different‑sized cubes of agar containing bromothymol blue
indicator. The student placed the cubes in hydrochloric acid and measured the time taken for
each cube to become yellow.
Table 1.2
4 60
...........
6 216 170
8 512 275
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7
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(ii) Plot a line graph on the grid of the length of the cube sides against the time taken for the
cubes to become yellow.
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[4]
(iii) Use your graph to estimate the time taken for a cube with a side length of 3 mm to
become yellow.
.............................................................. s
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[2]
(iv) State two variables that should have been kept constant in this investigation.
1 ........................................................................................................................................
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2 ........................................................................................................................................
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[2]
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[Total: 21]
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A B
[4]
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Calculate the magnification of the seedling in Fig. 2.1 using the formula and your
measurement.
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[3]
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Fig. 2.2
similarity 1 ..........................................................................................................................
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difference 1 ...........................................................................................................………..
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difference 2 ........................................................................................................................
Describe the methods you would use to test a sample of seedlings for starch and
reducing sugars.
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[3]
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11
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(c) When light shines from one side, seedlings grow towards the light.
Fig. 2.3 shows a diagram of a seedling growing towards a light source. The angle of growth is
shown on the diagram.
light source
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Fig. 2.3
Plan an investigation to find out how light intensity affects the angle at which the
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seedling grows.
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[Total: 19]
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Cambridge Assessment International Education is part of Cambridge Assessment. Cambridge Assessment is the brand name of the University of Cambridge
Local Examinations Syndicate (UCLES), which is a department of the University of Cambridge.
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