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Lab Report

Lab Report in BioChem

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Marvin Iloreta
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26 views

Lab Report

Lab Report in BioChem

Uploaded by

Marvin Iloreta
Copyright
© © All Rights Reserved
Available Formats
Download as PDF, TXT or read online on Scribd
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1. Why do you need specific restriction enzymes in recombinant technology?

Specific restriction enzymes are needed in recombinant DNA technology to ensure


accuracy and specificity in DNA manipulation. Restriction enzymes are proteins that cut
DNA at specific sequences, producing fragments with known sequences at each end. The
availability of different restriction enzymes and ligases allows for the transfer of specific
DNA sequences from one molecule to another.

2. Selection of a vector depends upon?

When selecting a vector, you should consider the purpose of the process and the size of
the DNA fragment you're working with:

a. Purpose
The type of vector you choose depends on the process you're using it for. For
example, cloning vectors are essential for cloning procedures.
b. DNA fragment size
Vectors should be small enough to manipulate easily. Large vectors can affect
replication and stability. Plasmids can typically handle inserts up to 15 kb.

Vectors are an important part of genetic engineering, as they move DNA fragments from
one cell to another. They have specific features that allow them to survive in the host cell
and carry gene sequences. Some vectors integrate into the host DNA, while others pass
the genetic material into the host cell and then recover themselves.

3. What are the main steps of cloning?

The main steps of DNA cloning are:

1. Isolate the DNA: Cut the DNA from its source


2. Insert the DNA into a vector: "Paste" the DNA into a DNA vector that can
replicate
3. Join the DNA and vector: Use ligation to join the ends of the DNA with the vector
DNA
4. Introduce the vector into a host cell: Introduce the vector into a host cell
5. Isolate and purify the vector DNA: Isolate the vector DNA from the host cells'
DNA and purify it

4. What is the shape of plasmid?


A plasmid is typically circular in shape, but some plasmids in bacteria can be linear. A
plasmid is a small, double-stranded DNA molecule that is separate from the
chromosomal DNA of a cell.

5. How do you carry out colony selection?

Here are the steps for carrying out colony selection:

1. Identify a colony: Look for a well-isolated bacterial colony on the agar plate.
Avoid colonies that are clustered together.
2. Pick the colony: Use a sterile toothpick, pipette tip, or inoculation loop to pick up
the colony.
3. Transfer the colony: Put the colony into a cell culture medium, which can be
liquid or agar.
4. Incubate: Incubate the medium overnight to encourage bacterial growth.
5. Test the colonies: Test the resulting colonies to see if they meet the desired goal.

6. Why is colony selection important?

Colony selection is important because it allows researchers to identify and isolate


microorganisms for further study and experimentation.

7. Can you think of other applications of cloning related to mammals excluding DNA fragment
and mammalian cloning?

It can be used for conservation. Cloning endangered species or preserving genetic


diversity.

8. Do you support GM food or advocate against it? Give reasons for your choice.

Yes, I support it. Simply because I don’t really see any harm in doing so and we have
been eating genetically modified foods such as the likes of corn and papaya.

9. Do you think, as muslims, our ethics allow us to clone similar to the way 'Dolly was cloned?

Based solely on the brief research I did on the internet. It seems that to muslims animal
cloning is permissible so long as it does not involve harm or violate any Islamic laws and
is beneficial to humanity.

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