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perhitungan 3 ml

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13 views6 pages

perhitungan 3 ml

Uploaded by

Indaah S
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Lampiran 3

a. Penyiapan Larutan Baku


Larutan baku 500 ppm
500 mL
=
1000 mL
25 mg
=
50 mL
Jadi larutan baku 500 ppm terdiri dari 25 mg beta karoten murni dalam 50 mL pelarut
b. Perhitungan Kurva baku
Rumus : v1.c1 = v2.c2
50 x 5
 V1 = = 0,5 mL
500
50 x 10
 V1 = = 1 mL
500
50 x 15
 V1 = = 1,5 mL
500
50 x 20
 V1 = = 2 mL
500
50 x 25
 V1 = = 2,5 mL
500
c. Perhitungan Persamaan Regresi Linear
Rumus : Y = a + bx
 a = ¿¿
6,179 x 1.375− (75 ) x 96,455
=
5 ( 1.375 ) −( 75 ) 2

8.496,125−7.234,125
=
6.875−5.625

1.262
=
1.250

= 1,0096

Jadi nilai a = 1,0096

n ( ∑ XY )−( ∑ X ) (∑ Y )
 b=
n ( ∑ X 2 )−( ∑ X ) 2
5 ( 96,455 )−( 75 )( 6,179 )
=
5 ( 1.375 )−( 75 ) 2
482,275−463,425
=
6.875−5.625
18 ,85
=
1.250
= 0,0151
Jadi nilai b = 0,0151

Sehingga di dapat rumus regresi linearnya yaitu Y = 1,0096 + 0,0151x

Tabel 4. Nilai Absorbansi dari sampel A dan B

Sampel Berat Sampel Serapan (A)


(gram)
1,436
Daging Buah 20,013 gram 1,440
(A) 1,443
2,245
Kulit Buah 20,035 gram 2,309
(B) 2,343

d. Perhitungan kadar sampel


1. Perhitungan kadar sampel daging buah (A)
Diketahui :
Berat sammpel A : 20 gram
Y (absorban) : 1,436 , 1,440, 1, 443
Volume sampel : 3 mL
Faktor pengenceran (FP) ; 1 kali
Sehingga :
 Pengukuran 1
 Y = 0,0151x + 1,0096
1,436 = 0,0151x + 1,0096
1,436 – 1,0096 = 0,0151x
0,4264 = 0,0151x
0,4264
x=
0,0151
x = 28,238 ppm
x = 28, 238 µg/ml
kemudian :
C x Fp x V
Kadar =
Bs
28,238 x 1 x 3
Kadar =
20,013
84,714 µg/ml x 3 ml
Kadar =
20,013 gr
Kadar = 4,232 µg/g
 Pengukuran 2
 Y = 0,0151x + 1,0096
1,440 = 0,0151x + 1,0096
1,440 – 1,0096 = 0,0151x
0,4304 = 0,0151x
0,4304
x=
0,0151
x = 28,503 ppm
x = 28,503 µg/ml
kemudian :
C x Fp x V
Kadar =
Bs
28,503 µ /ml x 1 x 3 ml
Kadar =
20,013 g
85,509 µg
Kadar =
20 , 013 g
Kadar = 4,272 µg/g
 Pengukuran 3
 Y = 0,0151x + 1,0096
1,443 = 0,0151x + 1,0096
1,443 – 1,0096 = 0,0151x
0,4334 = 0,0151x
0,4334
x=
0,0151
x = 28,701 ppm
x = 28,701 µg/ml
kemudian :
C x Fp x V
Kadar =
Bs
28,701 µg /ml x 1 x 3 ml
Kadar =
20,013 g
86,103 µg
Kadar =
20,013 g
Kadar = 4,302 µg/g
2. Perhitungan kadar sampel kulit buah (B)
Diketahui :

Berat sammpel B : 20,035 gram

Y (absorban) : 2,245, 2,309, 2,343

Volume sampel : 3 mL

Faktor pengenceran (FP) ; 1 kali

Sehingga :

 Pengukuran 1
 Y = 0,0151x + 1,0096
2,245 = 0,0151x + 1,0096
2,245 – 1,0096 = 0,0151x
1,235 = 0,0151x
1,235
x=
0,0151
x = 81,814 ppm
x = 81,814 µg/ml
kemudian :
C x Fp x V
Kadar =
Bs
81 ,81 4 µg /ml x 1 x 3 ml
Kadar =
20,035 g
245,442 µg
Kadar =
20,035 g
Kadar = 12,25 µg/g
 Pengkuran 2
 Y = 0,0151x + 1,0096
2,309 = 0,0151x + 1,0096
2,309 – 1,0096 = 0,0151x
1,299 = 0,0151x
1,299
x=
0,0151
x = 86,052 ppm
x = 86,052 µg/g
kemudian :
C x Fp x V
Kadar =
Bs
86,052 µg /g x 1 x 3 ml
Kadar =
20,035 g
258,156 µg
Kadar =
20,035 g
Kadar = 12,885 µg/g
 Pengukuran 3
 Y = 0,0151x + 1,0096
2,343 = 0,0151x + 1,0096
2,343 – 1,0096 = 0,0151x
1,334 = 0,0151x
1,334
x=
0,0151
x = 88,344 ppm
x = 88,344 µg/g
kemudian :
C x Fp x V
Kadar =
Bs
88,344 µg/ g x 1 x 3 ml
Kadar =
20,035 g
265,032 µg
Kadar =
20,035 g
Kadar = 13,228 µg/g
3. Perhitungan kadar rata – rata sampel A dan B
Kadar rata-rata :
Kadar A 1+ Kadar A 2+ Kadar A 3
1. Kadar β-karoten sampel A =
3
4,232+ 4,272+ 4,302
=
3
12,806
=
3
= 4,268 µg/g

Kadar B1+ Kadar B 2+ Kadar B 3


2. Kadar β-karoten sampel B =
3
12, 25+12,885+13,228
=
3
38,363
=
3
= 12,787 µg/g

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