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Staining Notes - Microbe Notes

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Kedir Mohammed
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© © All Rights Reserved
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Microbe Notes

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Staining Topics / Categories


Agricultural Microbiology (12)
Staining is a biochemical process usually conducted to increase the contrast of a
Bacteriology (123)
microscopic image.
Basic Microbiology (60)
Staining samples with different dyes is an important part of microscopy where the color
Biochemical Test (114)
contrast of a magnified is increased to differentiate the stained portion of the image from
the rest. Biochemistry (155)

Microscopic techniques in hematology, histopathology, and clinical microbiology use Bioinformatics (21)

staining as a method to study and diagnose diseases at the microscopic level. Biology (183)
Dyes used in staining can be used to define the biological tissues, cell populations, or Biotechnology (28)
subcellular organelles. Book Reviews (1)
The purpose of staining might also differ as some staining procedures are used to Cell Biology (94)
differentiate between two groups of microorganisms (Gram Staining) while some are
Culture Media (66)
used to observe motility and presence of a capsule.
Difference Between (89)
Staining is a part of biochemistry where a class-specific dye is added to a substrate to
Diseases (29)
qualify or quantify the presence of a certain compound.
Environmental Microbiology (6)
Staining, however, is not limited to biological samples, as it can be used to study the
lamellar structure of polymers and the structure of block compounds. Epidemiology (23)

Bright field microscopes are commonly used to observe stained cells or other biological Food Microbiology (49)

materials to provide a distinction between the sample and the background. Genetics (78)

It is also difficult to observe colorless substances under a microscope, which is why the Human Anatomy and Physiology (70)
concept of staining is so popular. Immunology (115)
Staining is of different types depending on their purpose; simple staining using a single Instrumentation (63)
stain to provide a contrast between the sample and the background; differential staining
Journals (1)
using more than one dyes in order to differentiate between cells or between organelles
Microscopy (28)
of a cell.
Molecular Biology (66)
Among all the staining techniques, Gram staining is the most popular technique that are
Mycology (33)
used to differentiate between Gram-positive and Gram-negative microorganisms.
Parasitology (25)
Other staining techniques include endospore staining for endospores, acid-fast staining
for fastidious organisms, Periodic-acid Schiff (PAS) for biomolecules, and Romanowsky Pharmacology (14)

staining to differentiate between blood cells. Phycology (2)

Protocols (9)

Research Methodology (19)


Sudan Black B Staining: Principle, Procedure, Results, Uses
Staining (29)
January 28, 2023 by Faith Mokobi
Syllabus (18)

Sudan stain is a special stain used for staining of fats Universities (4)

and fat droplets using several Sudan dyes, which Virology (50)
include Sudan II, Sudan III, Sudan IV, Oil Red O, and
… Read more

Lactophenol Cotton Blue (LPCB) Staining


August 21, 2022 by Faith Mokobi

Lactophenol Cotton Blue (LPCB) Staining is a simple


histological staining method used for the
microscopic examination and identification of fungi.
Principle of Lactophenol Cotton Blue (LPCB) Staining
LEAM Solution Fluorescence Microscope
Lactophenol Cotton Blue … Read more

Giemsa Stain- Principle, Procedure, Results, Interpretation


August 2, 2022 by Faith Mokobi

Introduction Giemsa stain was a name adopted from


a Germany Chemist scientist, for his application of a
combination of reagents in demonstrating the
presence of parasites in malaria. It belongs … Read
more

Masson’s Trichrome Staining


July 28, 2022 by Faith Mokobi

Masson’s Trichrome Staining definition Masson’s


Trichrome Staining is a histological staining method
used for selectively stain collagen, collagen fibers,
fibrin, muscles, and erythrocytes. It uses three stains
for staining hence … Read more

Ziehl-Neelsen Staining- Principle and Procedure with Results


July 8, 2022 by Faith Mokobi

What is Ziehl-Neelsen staining? The Ziehl-Neelsen


Hyaline Casts: Structure, Types, Process,
staining technique is a differential staining technique
Importance
that was initially developed by Ziehl and modified
later by Neelsen, hence the name Ziehl-Neelsen Secondary Succession: Stages, Examples,
Importance
stain. Neelsen … Read more
Primary Succession: Stages, Examples,
Importance
Positive staining of Viruses Mucous Membrane: Structure, Location,
June 29, 2022 by Faith Mokobi
Functions, Disorders

Secondary Consumers: Types, Food Chain,


What is positive staining? Positive staining of viruses
Examples, Roles
is similar to negative staining. The difference is that
the virus image is darker formed on a light
background, unlike the negative … Read more

Wheatley Trichrome Staining


June 27, 2022 by Faith Mokobi

Wheatley Trichrome Staining definition Wheatley


Trichrome staining technique is a special permanent
satin used in parasitology for the detection and
identification of intestine protozoans from stool
samples. Trichrome staining is … Read more

Romanowsky Stains- Principle, Types, Applications


June 23, 2022 by Faith Mokobi

What are the Romanowsky Stains? Romanowsky


Stains are the stains that are used in hematology and
cytological studies, to differentiate cells in
microscopic examinations of blood and bone marrow
samples. … Read more

Periodic Acid-Schiff (PAS) Staining


June 22, 2022 by Faith Mokobi

What is Periodic Acid-Schiff (PAS) Staining? Periodic-


Acid Schiff (PAS) staining technique is used in
histochemistry and histological studies to
demonstrate the presence of carbohydrates and
carbohydrate compounds such as polysaccharides, …
Read more

Negative staining of Viruses


June 17, 2022 by Faith Mokobi

What is the Negative staining of Viruses? Staining


techniques are designed to show contrast under
microscopic observation. The staining of viruses is
done to increase the contrast between the virus …
Read more

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