Biological Test
Biological Test
June 2016
Biology Practical
P530/3
3 Hours
Instructions to candidates:
Question Marks
1
Total
a) Dissect the specimen to release the skin from the body wall on the ventral side;
a) Obtain a cube measuring 1x1x1cm from specimen P. Crush it to obtain an extract. Carry
out tests in table 1 below on both the extract and solution X. Record your tests,
observations and conclusions.
b) Obtain 4 test tubes labeled 1-4. To all add 3cm3 of X. Now obtain 4 cubes from specimen
P of dimensions 1x1x1cm and treat them as follows.
Cube 1: Boil for 1 minute in water, remove and cool
Cube 2: Cut into 2 pieces
Cube 3: Cut into 4 pieces
Cube 4: Leave intact
Transfer the cubes into the corresponding test tubes 1-4. Leave for one hour as you
continue with other tasks. After this time, crush the cubes/the pieces to obtain extracts 1-
4. Carry out a reducing sugar test on extracts and record your observations in the table 2
below (12 marks)
Table 2
………………………………………………………………………………………………
d) Give precise explanations for the observations made in tests tubes 1-4 as informed by the
process stated in c(i) above. (9 marks)
i) Test tube 1
………………………………………………………………………………………
………………………………………………………………………………………
………………………………………………………………………………………
………………………………………………………………………………………
ii) Test tube 2
………………………………………………………………………………………
………………………………………………………………………………………
………………………………………………………………………………………
………………………………………………………………………………………
iii) Test tube 3
………………………………………………………………………………………
………………………………………………………………………………………
………………………………………………………………………………………
………………………………………………………………………………………
iv) Test tube 4
………………………………………………………………………………………
………………………………………………………………………………………
………………………………………………………………………………………
………………………………………………………………………………………
………………………………………………………………
You are provided with specimens A, B, C, D and E. Make biological examination of the
specimens using a hand lens and answer the following questions
a) Identify the specimens and place them into one major group. (1 mark)
…………………………………………………………………………………………
What diagnostic features have you used to give the specimens the identity in (a)
above? (3 marks)
…………………………………………………………………………………………
…………………………………………………………………………………………
…………………………………………………………………………………………
b) Complete the following table by giving the salient description of the structures
indicated in table 3 below (12 ½ marks)
Table 3
c) Using structural features of the wings, construct a dichotomous key to identify the
specimens (5 marks)
…………………………………………………………………………………
…………………………………………………………………………………
…………………………………………………………………………………
…………………………………………………………………………………
…………………………………………………………………………………
…………………………………………………………………………………
…………………………………………………………………………………
d) Observe the tarsal region of specimen B under low power of the microscope:
ii) Relate the structure of the tarsal region to the survival of the specimen in its
habitat. (3 marks)
…………………………………………………………………………………
…………………………………………………………………………………
…………………………………………………………………………………
…………………………………………………………………………………
END
Instructions to the teacher responsible for setting up the practical
6 test tubes
Butterfly labeled D
Cockroach labeled E
A hand lens