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Seat No.: _____ Enrolment No.

_____________

GUJARAT TECHNOLOGICAL UNIVERSITY


M.PHARM SEMESTER-1 EXAMINATION – SUMMER-2024

Subject Code: MAT101T Date: 07/06/2024


Subject Name: Modern Pharmaceutical Analytical Techniques
Time: 02:30 PM TO 05:30 PM Total Marks:80
Instructions:
1. Attempt any five questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.

Q.1 (a) Explain in detail instrumentation of UV-Visible spectrophotometer 06


(b) Write a brief note on theory and application of flourimetry 05
(c) Discuss the factors affecting fluorescence intensity 05
Q.2 (a) Write short note on detectors used in IR spectroscopy 06
(b) Discuss methods for sample preparation in IR spectroscopy 05
(c) Explain the principle, working, and advantages of FTIR 05
Q.3 (a) Enumerate ionization sources of Mass spectroscopy and discuss any one in 06
detail
(b) Discuss in detail about Quadrupole Analyzer 05
(c) Discuss Mc-Lafferty rearrangement 05
Q.4 (a) Explain in brief theory and principle of NMR spectroscopy 06
(b) What is Chemical shift? Write factors affecting on chemical shift. 05
(c) Difference between Atomic Absorption and Atomic Emission Spectroscopy. 05
Q.5 (a) Give difference between: 1) Guard column and Analytical column 2) Normal 06
phase and Reverse Phase chromatography.
(b) Write down the principle and applications of HPLC 05
(c) Write short note on stationary phase and mobile phase of Gas chromatography 05
Q. 6 (a) What is Chromatography? Enumerate different types of chromatography 06
techniques. Write the principle of ion-exchange chromatography
(b) Write short note on Affinity chromatography 05
(c) Discuss about Thin Layer chromatography 05
Q.7 (a) What is electrophoresis? Give different types of electrophoresis. Explain any 06
one in detail.
(b) Write a short note on Size exclusion chromatography 05
(c) Write a short not on X ray diffraction methods and Braggs law 05

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