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Biotech. & Application Revision Test

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Mohd Arhan Ali
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13 views

Biotech. & Application Revision Test

Uploaded by

Mohd Arhan Ali
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOCX, PDF, TXT or read online on Scribd
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CLASS REVISION TEST

CH. BIOTECHNOLOGY AND ITS APPLICATION

1. Microparticles for coating with DNA to be bombarded with gene gun is made up of
(a) platinum or zinc. (b) silicon or platinum. (c) silver or platinum. (d) gold or
tungsten
2. The example of a natural genetic engineer is
(a) Bacillus subtilis. (b) Salmonella typhimurium. (c) Agrobacterium tumefaciens
(d) Bacillus amyloliquefaciens
3. The process of recombinant DNA technology has following steps
I. Amplification of gene
II. Insertion of recombinant DNA into the host cell.
III. Cutting of DNA at specific location using restriction enzyme
IV. Isolation of genetic material (DNA).
Pick out the option for the correct sequence of step for the process of recombinant DNA
technology.
(a) II, III, IV and I. (b) IV, II, III and I. (c) I, II, III and IV. (d) IV, III, I and II
4. Which of the following is not correctly matched for organism and its cell wall degrading
enzyme?
(a) Algal-methylase. (b) Bacteria-lysosyme. (c) Fungi-chitinase. (d) Plant cells-cellulase
5. What is the criterion for DNA fragments movement on agarose gel during gel
electrophoresis?
(a) The smaller the fragment size, the farther it moves
(b) Negatively charged fragments do not move
(c) Positively charged fragments move to the farther end
(d) The larger the fragment size, the farther it moves
6. Assertion (A) Biotechnology deals with techniques that use living organism to produce
products useful for humans.
Reason (R) It uses only a unicellular organism.
7. Assertion (A) The first restriction endonuclease was Hind II.
Reason (R) The Hind II always cut DNA molecule at particular point by recognising a
specific sequence of 6-base pair.
8. Assertion (A) Restriction enzymes cut the strand of DNA to produce sticky ends.
Reason (R) Stickiness of the end facilitates the action of enzyme DNA polymerase.
9. Assertion (A) In EcoRI, the letter 'R' derived from the order.
Reason (R) Exonuclease and endonuclease are the types of nucleases enzyme.
10. Assertion (A) Foreign DNA and vector DNA cut with the help of ligase.
Reason (R) Ligase acts by forming phosphodieste bonds.
11.Assertion (A) E. coli having pBR322 with DNA insert at BamHI site cannot grow in
medium containing tetracycline.
Reason (R) Recognition site for BamHI is present in tet region of pBR322
12.(i) State the principle involved in separation of DNA fragments using gel electrophoresis.
(ii) How are DNA fragments visualised once they are separated by gel
electrophoresis?(2)
13.Expand 'BAC' and 'YAC'. What are they and what is the purpose for which they are
used?(2)
14. What essential features must be present in a cloning vehicle/cloning vector? (3)
15. Describe the process of amplification of 'gene of interest' using PCR technique. (3)
16. Explain the contribution of Thermus aquaticus in the amplification of a gene of
interest.(3)
17. Describe the roles of (i) high temperature, (ii) primers and (iii) bacterium- Thermus
aquaticus in carrying the process of polymerase chain reaction. (3)
18. Explain incessional inactivation.(3)

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