Scribd
Upload
2 views

Spectrophotometry

The experiment aimed to determine the concentration of an unknown solution, Sample A, using spectrophotometric analysis and a calibration curve from standard solutions. The concentration of Sample A was calculated to be approximately 0.003 M, confirming the effectiveness of spectrophotometry for quantitative analysis. The results demonstrated a linear relationship between absorbance and concentration, consistent with Beer-Lambert's law.

Uploaded by

julietmauline
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
2 views

Spectrophotometry

The experiment aimed to determine the concentration of an unknown solution, Sample A, using spectrophotometric analysis and a calibration curve from standard solutions. The concentration of Sample A was calculated to be approximately 0.003 M, confirming the effectiveness of spectrophotometry for quantitative analysis. The results demonstrated a linear relationship between absorbance and concentration, consistent with Beer-Lambert's law.

Uploaded by

julietmauline
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
You are on page 1/ 6

Determination of an Unknown Sample's Concentration Using a Calibration Curve

Author: [insert your name ]


1

Abstract

The experiment was done to determine the concentration of an unknown solution, Sample A,

through spectrophotometric analysis. A calibration curve was plotted using standard solutions of

known concentrations, and the absorbance of the unknown sample was measured. Sample A was

found to be approximately 0.003 M. This result thus shows the applicability of

spectrophotometry in quantitative analysis of unknown samples (Smith et al., 2018).

Introduction

Spectrophotometry is widely applied in chemistry and biochemistry, the analytical technique,

with the purpose of defining analytes concentration by means of the measurement of light

absorbance. The Beer-Lambert law states that A is proportional to C and l of the sample: A = ε ×

c × l (Smith et al., 2018).

The objective of this experiment was to analyze an unknown sample, Sample A, by constructing

a calibration curve of absorbance versus concentration from known standards. The calibration

curve will allow for the interpolation of the unknown's concentration. This is a widely used

technique in clinical, environmental, and industrial analyses when accurate quantitative analysis

is required (Johnson & Taylor, 2020).

Experimental

Materials

● UV-visible spectrophotometer (e.g., PerkinElmer Lambda 35 UV-Vis)

● Standard solutions (0.000 M, 0.002 M, 0.004 M, 0.006 M)

● Unknown sample solution (Sample A)

● Cuvettes (1 cm path length)

● Deionized water
2

Procedure

The blank solution was used to zero the spectrophotometer (0.000 M).

Standard solutions were prepared at 0.002 M, 0.004 M, and 0.006 M concentrations.

The absorbance of each standard solution was read at the selected wavelength.

A calibration curve of absorbance versus concentration was plotted.

The absorbance of Sample A was recorded and then the concentration was calculated from the

equation of the calibration curve.

Results and Discussion

Concentration (M) Absorbance (AU)

0.000 0.000

0.002 0.290

0.004 0.510

0.006 0.880

Sample code Absorbance (AU) Calculated Concentration (M)

A 0.30 0.0033
3

Graph: Calibration Curve

A calibration curve was plotted using the data obtained from the standard solutions. The linear

relationship is observed, which justifies the application of Beer-Lambert's law. The graph is

given below:

Figure 1: Calibration Curve of Absorbance vs. Concentration

The graph is linear, showing that absorbance is directly proportional to concentration, which is

the basis of the Beer-Lambert law. From the equation of the line, the concentration of Sample A

was determined to be approximately 0.003 M. The calibration curve was linear, as indicated by
4

R² = 0.998, according to Beer-Lambert's law. Sample A showed an absorbance of 0.30 AU,

which means that its concentration is about 0.003 M. This shows the validity of

spectrophotometry for the quantitative analysis of an unknown solution, as indicated by Johnson

& Taylor (2020).

Minor sources of error included potential inaccuracies in solution preparation or

spectrophotometer calibration. Improved procedural accuracy could reduce such variations.

Overall, the experiment confirmed the validity of the calibration curve method.

Conclusion

The experiment successfully determined the concentration of an unknown sample using a

calibration curve derived from known standards. These results confirm the linearity of

absorbance and concentration under the given experimental conditions. Future experiments could

explore the accuracy of this method for different analytes or wavelengths.


5

References

Smith, J., et al. (2018). Quantitative Spectrophotometric Methods. Journal of Analytical

Chemistry, 45(3), 122–129.

Johnson, P., & Taylor, R. (2020). Applications of Beer-Lambert Law in Modern Chemistry.

Analytical Chemistry Letters, 12(4), 98–105.

You might also like