Auto Hematology Analyzer

SERVICE MANUAL

www.aehealthgroup.com
 CONTENTS
CORYRINGHT AND STATEMENT .................................................................................................................. 1
Chapter 1 Product specifications .......................................................................................................................1
1.1 PRODUCT NAME AND TYPE ............................................................................................................ 1
1.1.1 Name ............................................................................................................................................... 1
1.1.2 Model .............................................................................................................................................. 1
1.2 INTENDED USE ....................................................................................................................................1
1.3 SYSTEM STRUCTURE ........................................................................................................................ 1
1.3.1 Configuration ...............................................................................................................................1
1.3.2 Interfaces ......................................................................................................................................1
1.4 ELECTRICAL CHARACTERISTICS .................................................................................................. 1
1.5 SIZE AND WEIGHT ..............................................................................................................................1
1.6 ANALYSIS PARMTERS ....................................................................................................................... 2
1.7 PERFORMANCE SPECIFICATIONS .................................................................................................. 3
1.8 TRANSPORTATION AND STORAGE CONDITIONS .......................................................................4
1.9 IMPORTANT NOTE .............................................................................................................................. 4
1.10 FUNCTIONS ........................................................................................................................................... 5
1.10.1 Analysis mode ..........................................................................................................................5
1.10.2 Sample size required for each sampling ..................................................................................5
1.10.3 Detection Speed ....................................................................................................................... 5
1.10.4 Calibration&QC .......................................................................................................................5
1.10.5 Auto cleaning and auto unblocking functions of sample aspirating probe and automatic re
counting after blockage removal ............................................................................................................. 5
1.10.6 Sleep and awake functions ...................................................................................................... 6
1.10.7 Functions of auto intetized diagnosis,mainenance and maintenance logbook records .......... 6
1.10.8 Screen keyboard,mouse,standard keyboard,online Chinese input funchtion ......................... 6
1.10.9 Display and print ..................................................................................................................... 6
1.10.10 Reference value and abnormal result warning system ............................................................6
1.10.11 LIS Function ............................................................................................................................ 6
1.10.12 Storage ..................................................................................................................................... 6
1.10.13 Optional functions of the analyzer .......................................................................................... 6
1.10.13.1 Bar code reader ..................................................................................................................... 7
1.10.13.2 External recorder .................................................................................................................. 7
Chapter 2 Principle ................................................................................................................................................8
2.1 INTRODUTION .....................................................................................................................................8
2.2 ASPIRATION SAMPLES ...................................................................................................................... 8
2.3 DILUTION OF SAMPLES .................................................................................................................... 8
2.4 WHITE BLOOD CELL MEASUREMENTS ........................................................................................8
2.4.1 Laser flow cytometry ................................................................................................................8
2.4.2 White blood cell parameters .....................................................................................................9
2.5 HEMOGLOBIN CONCENTRATION MEASUREMENT .................................................................10
2.5.1 Colorimetric method ............................................................................................................... 10
2.5.2 Hemoglobin concentration parameters ...................................................................................11
2.6 RED BLOOD CELL/PLATELET MEASUREMENTS .......................................................................11
2.6.1 Principle of impedance method ................................................................................................ 11
2.6.2 Red blood cell parameters ........................................................................................................ 12
2.6.3 Platelet parameters .................................................................................................................... 12
Chapter 3 Software and interface ................................................................................................................... 14
3.1 POWER ON .............................................................................................................................................14
3.1.1Version check .................................................................................................................................14
3.2 LOGIN ..................................................................................................................................................... 14
3.2.1 User name and password .............................................................................................................. 14
3.2.2 Login check .................................................................................................................................. 14
3.3 CALIBRATION .......................................................................................................................................14
3.3.1 Calibration factors and transfer factors ........................................................................................ 14
3.3.2 Calibration with calibrator ............................................................................................................15
3.4 GAIN CALIBRATION ............................................................................................................................15
3.5 SOFTWARE INSTALLATION AND UPGRADATION ....................................................................... 16

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 3.6 STATUS INDICATOR ............................................................................................................................ 16
Chapter 4 Hardware system ............................................................................................................................17
4.1 INTRODUCTION OF HARDWARE .................................................................................................. 17
4.2 MAIN CONTROL BOARD MODULE ...............................................................................................17
4.2.1 The main input signal of the motherboard ................................................................................... 17
4.2.2 The main output signal of the motherboard ................................................................................. 18
4.2.3 Functionality and performance implementation .......................................................................... 18
4.2.4 Introduction to the module circuit ................................................................................................ 18
4.2.5Common faults and analysis ..........................................................................................................20
4.3 SIGNAL BOARD MODULE ...............................................................................................................22
4.3.1 The main input signal of the signal board .................................................................................... 22
4.3.2 The main output signal of the signal board .................................................................................. 22
4.3.3 Functionality and performance implementation .......................................................................... 22
4.3.4 Introduction to the module circuit ................................................................................................ 23
4.3.5Common faults and analysis ..........................................................................................................25
4.4 DRIVER BOARD MODULE .............................................................................................................. 26
4.4.1 The main input signal of the signal board .................................................................................... 26
4.4.2 The main output signal of the signal board .................................................................................. 26
4.4.3 Functionality and performance implementation .......................................................................... 26
4.4.4 Introduction to the module circuit ................................................................................................ 27
4.4.5Common faults and analysis ..........................................................................................................28
4.5 WBC OPITAL SIGNAL AMPLIFICATION BOARD MODULE ......................................................31
4.5.1 Functionality and performance implementation .......................................................................... 31
4.5.2 Introduction to the module circuit ................................................................................................ 31
4.5.3Common faults and analysis ..........................................................................................................32
4.6 LASER DRIVER BOARD MODULE ................................................................................................ 32
4.6.1 Functionality and performance implementation .......................................................................... 32
4.6.2 Introduction to the module circuit ................................................................................................ 33
4.7 HIGH-ANGULE PD BOARD MODULE ........................................................................................... 33
4.7.1 The main input signal of the High-angule PD board ................................................................... 33
4.7.2 The main output signal of the High-angule PD board ................................................................. 33
4.7.3 Functionality and performance implementation .......................................................................... 33
Chapter 5 Fault alarm information ................................................................................................................... 35

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 COPYRIGHT
Copyright

AEHEALTH LIMITED. (following referred to as "AEHEALTH") owns the intellectual

property rights to this operation manual. This manual may refer to information protected by

copyright or patents and does not convey any license under the patent rights or copyright of

AEHEALTH, or of others. The intellectual property rights of the MANUAL and the

corresponding product belong to AEHEALTH .

This manual and its contents represent special and confidential information of AEHEALTH.

Disclosure of the information in this manual in any manner whatsoever without the written

permission of AEHEALTH is strictly forbidden. Release, amendment, reproduction, distribution,

rental, adaptation, translation or any other derivative work of this manual in any manner

whatsoever without the written permission of AEHEALTH is strictly forbidden.

“ ” is the trademarks of AEHEALTH LIMITED. The product name of

non-AEHEALTH may be the trademarks of correspondung owners.

Statement

AEHEALTH reserves the final right to interpret this manual. In the event that all of the

following requirements are met, AEHEALTH is responsible for the safety, reliability and

performance of the product:

1.The assembly, extensions, readjustments, modifications, and repairs are performed

by professionals authorized by AEHEALTH.

2. All replacement parts used in the repairs and all accessories and consumables used are

products of or approved by AEHEALTH.

3. Relevant electrical equipment conforms to national standards and the requirements of this

manual.

4.The operation of the product shall be carried out in accordance with this manual.

1
 CHAPTER 1 Product specifications

1.1 PRODUCT NAME AND TYPE

1.1.1 Name
Auto 5-Diff Hematology Analyzer

1.1.2 Model
AerC-5

1.2 INTEND USE

AerC-5 Auto 5-Diff Hematology Analyzer is a kind of in vitro diagnostic
equipment used in clinical laboratory for hemoglobin test, blood cell count&analysis,
and WBC 5-part classification count of human blood sample .

1.3 SYSTEM STUCTURE

1.3.1 Configuration
Standard configuration
Instrument packing list is as the Appendix

1.3.2 Interfaces
1 LAN net gape
4 USB interface

1.4 ELECTRICAL CHARCTERISTICS

Parameters Values
Voltage (100~240) V±10% V
Frequency 50Hz/60 Hz±1Hz
Input power ≤180VA

1.5 SIZE AND WEIGHT

L (mm) ≤330
H (mm) ≤565
D (mm) ≤470
Weight (Kg) ≤30

1.6 ANALYSIS PARAMETERS

The analyzer outputs a total of 25 report parameters, 7 research parameters, 3 histograms,
and 1 scatter plot. The corresponding parameters in CBC, CBC+DIFF measurement
modes are detailed in the table below:

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Parameters Name Abbreviations CBC CBC+DIFF
White blood cell count WBC √ √
Basophil count Bas# / √
Basophil percentage Bas% / √
Neutrophil count Neu# / √
Neutrophil percentage Neu% / √
White blood Eosinophil count Eos# / √
cell lines Eosinophil percentage Eos% / √
( 15items), Lymphocyte count Lym# / √
Including 4 Lymphocyte percentage Lym% / √
Monocyte count Mon# / √
reportable
Monocyte percentage Mon% / √
parameters
atypical lymphocyte count ALY# / √
Atypical lymphocyte percentage ALY% / √
Large number of immature cells LIC# / √
count
Large number of immature cells LIC% / √
percentage
Red blood cell count RBC √ √
Hemoglobin concentration HGB √ √
White blood Mean corpuscular volume MCV √ √
cell lines Mean corpushcular hemoglobin MCH √ √
(10 items) Mean corpushcular hemoglobin MCHC √ √
Including 2 concentration
reportable Red blood cell distribution RDW-CV √ √
parameters width-Coefficient of Variation
Red blood cell distribution RDW-SD √ √
width-Standard Variation
Hematocrit HCT √ √
Nucleated redblood cells count *NRBC# √ √
Nucleated redblood cells *NRBC% √ √
percentage
Platelet Platelet count PLT √ √
group(7 Mean platelet volume MPV √ √
items) Platelet distribution width PDW √ √
Including 1 Plateletcrit PCT √ √
reportable Large platelet ratio P-LCR √ √
parameter Large platelet count P-LCC √ √
Platelet Clumps count *PLT Clumps# √ √
WBC Histogram √ √
Histogram RBC Histogram √ √
PLT Histogram √ √
Diff Scattergram / √

Notice
● "√" indicates provided in this measurement mode, and "/" indicates not provided in
this measurement mode.

● ALY%, LIC%, ALY #, LIC #, * NRBC #, * NRBC%, * PLT Clums # are research
parameters and are only used for research purposes and cannot be used as a basis for
clinical diagnosis.

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1.7 Performance Specifications
a. Blank Count

Parameters Blank Counting Requirements

WBC ≤ 0.20× 109 /L
RBC 12
≤ 0.02× 10 /L
HGB ≤ 1g/L
PLT ≤ 10×109 /L

b. Linearity Range
Deviation Linearity
Parameters Linearity Range correlation
Range coefficient
(Whole
Blood)
0.00×109 /L～10.00×109 /L
±0.30× 109 /L
WBC 10.1 × 109 /L～100.0×109 /L ≥0.990
±5%
0.00× 1012 /L～1.00× 1012 /L
±0.05× 1012 /L
RBC 1.01× 1012 /L～8.00×1012 /L ≥0.990
±5%

0 g/L～70g/L ±2g/L
HGB ≥0.990
71 g/L～250g/L ±2%

0 × 109 /L～100× 109 /L ±10× 109 /L

PLT 101× 109 /L～1000× 109 /L ≥0.990
±8%

c. Accuracy
Allowable relative
Parameters Measuring Range
deviation range
WBC 3.5 × 109 /L～9.5× 109 /L ±10%

RBC ±4%
3.8 × 1012 /L～5.8× 1012 /L

HGB 115 g/L～175 g/L ±4%

PLT ±15%
125× 109 /L～350× 109 /L

MCV 82 fL to 100 fL ±5%

d.Repeatability(Better to use middle level quality control or samples

3
 Parameters Measuring Range Accuracy

WBC 3.5× 109 /L～15.0× 109 ≤2.0%

/L
3.50× 1012 /L ~
RBC ≤1.5%
6.00× 1012 /L

HGB 110 g/L ~ 180 g/L ≤1.5%

MCV 70 fL～120 fL ≤1.0%

100× 109 /L ~ 149× 109 /L ≤6.0%

PLT 150×109 /L ~ 500×109 /L
≤4.0%

e.Carryover

Parameters Carryover
WBC ≤0.5%
RBC ≤0.5%
HGB ≤0.6 %
PLT ≤1.0 %

1.8 Analyzer Storage Environment

Ambient temperature range: -10℃～40℃
Relative humidity range: 10%～90% RH
Atmospheric pressure range: 50.0kPa～106.0kPa

1.9 Important
To keep your instrument up and running for as long as possible, be sure to do the
following：
1 Analyzer Operating Environment：
a．Ambient temperature range：10℃～30℃
b．Relative humidity range：≤ 85% RH
c．Atmospheric pressure range：70.0 kPa～106.0 kPa
d．Avoid excessive dust entering the inside of the instrument
e．Keep the analyzer away from strong sources of electromagnetic interference

2 Stable Power Supply

For analyzers working in areas with unstable power supply or frequent power outages, it
is strongly

recommended that the analyzer be equipped with an uninterruptible power

supply (UPS) or a regulated power supply.

4
 3 Grounding
Use the matching special ground wire to ground the analyzer well
4 Maintenance
a．Periodically remove dust adhering to the outside and inside of the analyzer
b．Maintain sample cups, valves and tubing with concentrated cleaning solution
during each shutdown
c．Atmospheric pressure：70.0 kPa～106.0 kPa
d．Keep the analyzer away from strong sources of electromagnetic interference

1.10 Function
1.10.1 Analyzer Mode
It can provide venous blood, peripheral blood test ,pre-dilution test;

1.10.2 Sucking Sample Volume

Test Mode CBC CBC+DIFF

Whole blood Mode 20 μL 20 μL
Pre-Diluted Mode 224 μL(Mixture sample: 20 μL peripheral blood +480uL diluent reagent

1.10.3 Throughput

Whole blood Mode 60T/hour

Pre-Diluted Mode 60T/hour

1.10.4 Calibration and QC

Quality controls and calibrators are used to perform calibration and quality control of the analyzer.
The "quality control substances" and "calibration substances" mentioned in this instruction manual
refer to the special quality control substances and calibration substances specified by AEHEALTH, which
should be purchased from AEHEALTH designated agent or AEHEALTH.

1.10.5 Aspirating needle automatic cleaning, automatic blockage

removal and automatic recount function after blockage removal
After aspirating the sample, the analyzer can automatically clean the sample
remaining on the outside of the tube wall of the aspirating needle.
A variety of automatic blocking functions, recoil, immersion and high pressure
burning.
After the blockage is removed, the instrument automatically re-analyzes the blood
sample: cell re-counting.

1.10.6 Sleep and wake function

When the analyzer does not have any operation within the specified time, the
aspirating needle will retract to avoid long-term exposure of contamination. And enter
5
the sleep state, press the sample key to wake up the sleep and enter the ready state.

1.10.7 Maintenance and establishment of maintenance log function

In case of improper operation or failure, the analyzer will automatically prompt the
relevant failure and prompt how to deal with it. In the analyzer menu, there is an
independent maintenance menu for users to call when dealing with related problems.
When the relevant maintenance and maintenance procedures have been carried out,
and the fault has been prompted, the analyzer will automatically record the information,
and automatically create a log table of maintenance, maintenance, and faults for users or
engineers and technicians to refer to and check.

1.10.8 Soft keyboard、Mouse、Keyboard

Support soft keyboard, mouse, keyboard；

1.10.9 Display、Printer
The display interface supports arbitrary switching of multiple languages: Chinese,
English, Spanish;
Both built-in and external recorders have printing versions in the above 3 languages;
can automatically detect paper loading and paper shortage prompts;
Various statistical reports of test functions can be printed;

1.10.10 Reference value and abnormal result alarm system

Reference value ranges for different normal groups can be provided: general, man,
woman, child, baby;
Multiple : multilingual 、 report formats;
When the test result is abnormal, the analyzer will prompt the corresponding alarm
for reference.

1.10.11 LIS Function

Various data of the test can be transmitted to an external computer or LIS system
through LAN port；

1.10.12 Storage
Mass storage of test results, at least 50,000 sample analysis results including
parameter results 、histograms、patent and alarm information etc；

1.10.13 Optional features of the analyzer

According to the different customers, the optional functions of the analyzer are as
follows.

1.10.13.1 Barcode Reader

Support USB interface barcode scanner; when the barcode scanner is connected, the
analyzer automatically collects sample information and manages it;
6
1.10.13.2 External Recorder
1.10.13.2.1 Can support buit-in printer：SP-RMD8C；
1.10.13.2.2 Can support ordinary printer：HP LaserJet P1108；
1.10.13.2.3 Can support laser printer：HP M403D、Samsung M3825D、HP M401D；
1.10.13.2.3 Can support external thermal printer：WH-P03

7
 Chapter 2 Principle
2.1 INTRODUCTION
This chapter explains the principal of the analyzer. This analyzer uses the Coulter

principle to detect the number of RBC and PLT as well as their volume distribution;

the colorimetric method to measure HGB concentration; and the semiconductor laser

flow cytometry technique to obtain a statistical count of WBC in 5 Diff; the above

usually becomes CBC+Diff, abbreviated as " CD". On top of this, the analyzer calculates

the results of the remaining parameters.

2.2 ASPIRATION SAMPLES

In whole blood working mode, the analyzer will aspirate 20 μL of whole blood sample.

2.3 DILUTION OF SAMPLES

The sample to be tested is aspirated to the host and then subjected to different
reagents in a dilution process to form the test sample for blood routine test (red blood
cell/platelet measurement, white blood cell count/hemoglobin measurement, white blood
cell sorting measurement).

2.4 WHITE BLOOD CELL MEASUREMENTS

2.4.1 Laser Flow Cytometry

Figure 3- 1 white blood cell measurement

After the Lyse is mixed with the blood sample, the red blood cells are lysed and the white
8
blood cells are stained. The stained white blood cells and red blood cell fragments are

injected through a sample needle into a flow chamber filled with diluent. Under the sheath

formed by the diluent, the cells are accelerated twice and arranged in rows across the

laser detection area. The scattered light generated by the exposure of the cells to the laser

beam correlates with the cell size, the refractive index of the cell membrane and the

internal cell structure. The photodiode receives these scattered light signals and converts

them into electrical pulses. Based on the collected data of these electrical pulses, a

two-dimensional distribution of blood cell size and internal cell information, called a

scatter plot, can be obtained, as shown in Figure 3-2. The horizontal coordinate reflects

the internal complexity information of the cell, and the vertical coordinate

reflects the volume of the cell.

Figure 3-2 White blood cell scatter plot

2.4.2 white blood cell parameters

The analyzer obtained the lymphocyte percentage (Lym%), neutrophil percentage (Neu%),
monocyte percentage (Mon%), and eosinophil percentage (Eos%) by analyzing the DIFF
channel scatter plot and the Lym region, Neu region, Mon region, and Eos region therein.
The number of lymphocytes (Lym#), neutrophils (Neu#), monocytes (Mon#), and
eosinophils (Eos#) were then calculated in combination with the number of white blood
cells obtained from the BASO channel. Basophil count (Bas#) can be obtained directly

from the BASO channel. Cell counts are all in units of 109 /L.

A) White blood cell count

The analyzer obtains the white blood cell count (WBC) by directly measuring the
number of pulses corresponding to white blood cells in the BASO channel.

B) Basophil count
The analyzer obtains the white blood cell count (Bas#) by directly measuring the
number of

9
 pulses corresponding to basophils in the BASO channel.
C) Basophil percentage
Bas% = Bas# / WBC * 100%
D) Percentage of lymphocytes
Lym% = the number of particles in the DIFF channel that fall in the Lym
region / the sum of the counts of all particles in the DIFF channel except for the blood shadow
region * 100%

E) Neutrophil percentage
Neu% = number of particles in the DIFF channel that fall in the Neu region /
sum of the counts of all particles in the DIFF channel except for the blood shadow
region * 100%

F) Percentage of monocytes

Mon% = number of particles in the DIFF channel that fall in the Mon region /
sum of the

counts of all particles in the DIFF channel except for the blood shadow region *
100%

G ) Eosinophil percentage
Eos% = number of particles in the DIFF channel that fall in the Eos region /
sum of the counts of all particles in the DIFF channel except for the blood shadow
region * 100%

H) Number of lymphocytes
Lym# = WBC * Lym%
I) Neutrophil count
Neu# = WBC * Neu%
J) Number of monocytes
Mon# = WBC * Mon%
K) Eosinophil count
Eos# = WBC * Eos%

2.5 HEMOGLOBIN CONCENTRATION MEASUREMENT

2.5.1 Colorimetric method

When the diluted sample is added to the Lyse, the red blood cells lyse and release
hemoglobin, which binds to the Lyse and forms a hemoglobin complex. According to
Lambert's law, the transmitted light intensity of the hemoglobin complex solution and the
background case can be measured under monochromatic light irradiation of LED with a

10
central wavelength of 530 nm, and the hemoglobin concentration can be obtained by
calculation.
2.5.2 Hemoglobin concentration parameters
The hemoglobin concentration (HGB) is calculated in g/L by the following formula.
HGB = Constant * Ln (background transmitted light intensity / sample transmitted light
intensity)

2.6 RED BLOOD CELL/PLATELET MEASUREMENT

2.6.1 Principle of impedance method

Figure 3- 3 Counting schematic

The analyzer uses the principle of impedance method to achieve red blood cell/platelet
count. The RBC counting cell has a small opening called the test orifice. There are a pair
of positive and negative electrodes on both sides of the small hole, which are connected
to a constant current power supply. Since cells are poor conductors of electricity, the
resistance between the electrodes changes when cells in a diluted sample pass through
the test orifice under constant negative pressure, resulting in a pulse signal at both ends
of the electrodes proportional to the size of the cell volume. The number of pulses is equal
to the number of cells passing through the pore, and the amplitude of the pulse is
proportional to the volume of the cells.

The number of electrical pulses whose amplitude falls within the red blood cell/platelet
channel is calculated by comparing the amplification of the acquired electrical pulses with
the channel voltage threshold corresponding to the normal red blood cell/Platelet Volume
range. Thus, all collected electrical pulses are classified according to different channel
voltage thresholds, and the number of electrical pulses falling in the red
blood cell/platelet channel is the number of red blood cells/platelets. The
number of cells in each channel range based on pulse voltage amplitude
determines the volume distribution of cells. The histogram reflecting the distribution of

11
the cell population is a two-dimensional plot with horizontal coordinates indicating the
cell volume and vertical coordinates indicating the relative number of cells.

2.6.2 Red blood cell parameters

(A) Number of red blood cells

The analyzer obtains the red blood cell count (RBC) in units of 10 12 /L by directly
measuring the number of electrical pulses corresponding to the red blood cells.

(B) Mean red blood cell volume

From the Red blood cell Histogram, the mean red blood cell volume (MCV) was
calculated in fL.
(C) Hematocrit, mean red blood cell hemoglobin content, and mean red blood cell
hemoglogbin concentration.

The Hematocrit (HCT) in %; mean red blood cell hemoglobin content (MCH) in
pg; mean red blood cell hemoglobin concentration (MCHC) in g/L.

HCT = RBC * MCV / 10

MCH = HGB /RBC
MCHC = HGB/HCT * 100
(D) Coefficient of variation of red blood cell distribution width
The coefficient of variation of red blood cell distribution width (RDW-CV) was
obtained from the Red blood cell Histogram in %.

(E) Standard deviation of red blood cell distribution width

The standard deviation of red blood cell distribution width (RDW-SD) was
obtained by calculating the standard deviation of the red blood cell volume
distribution in fL.
2.6.3 Platelet parameters
A) Platelet count

The analyzer obtains the platelet count (PLT) in units of 109 /L by directly
measuring the number of electrical pulses corresponding to platelets.

(B) Mean Platelet Volume

The mean Platelet Volume (MPV) in fL was calculated from the histogram of
platelet distribution.

(C) Platelet distribution width

Platelet distribution width (PDW) was obtained from platelet histograms as the
geometric standard deviation of Platelet Volume distribution ( 10 GSD).

D) Plateletcrit
The analyzer calculates the Plateletcrit (PCT) in % by the following
12
 formula PCT = PLT * MPV / 10000

E) Number of large platelets

The analyzer obtains the number of large platelets (P-LCC) in 10 9/L by directly
measuring the number of electrical pulses corresponding to large platelets.

(F) Platelet-larger cell ratio

P-LCR = P-LCC / PLT * 100

13
 Chapter 3 Software and interface
3.1 POWER ON
3.1.1 Version check
When turned on, the machine will automatically check the software version matching. If
the software version does not match, a version matching window will pop up. Please prepare
the software upgrade package first, select the appropriate language, and click the "OK" button.
The system will execute the software upgrade process. The reason why the software version
mismatch is detected at startup is that the software components have been upgraded separately,
such as the digital version of FPGA writing software and driver. Dynamic board FPGA
writing software, MCU writing software, timing, system software

Notice
● Be sure to use controlled software upgrade packages when upgrading software.

3.2 LOGIN
3.2.1 User name and password
User name:Service
Password: 123456

3.2.2 Login check

Log in with user rights, and the machine will automatically detect the match between the
board backup data and the SD card data. If the board or SD card has been replaced, or the
configuration has been changed and the device is shut down abnormally, the user will be
prompted to restore or back up instrument data after logging in.
After clicking "OK", you will enter the data backup and recovery interface, and follow
the prompts to perform data backup and recovery operations:
1) If you replace the SD card with a new one, please follow the subsequent text
guidance and select "Restore" important parameters to the SD card.
2) If the main control board is replaced, please follow the subsequent text guidance and
select "Backup" to back up the data to the new main control board again.
3) If the configuration data has changed and the system shuts down abnormally, please
select "Backup" to back up the data to the new main control board again.

3.3 Calibration factors and transfer factors

The purpose of calibration is to obtain accurate blood sample analysis results.
The calibration method is to multiply the analysis results by the calibration coefficient so
that the final output analysis result is close to the target value. The calculation formula of the
calibration coefficient can be obtained as:

Calibration factor=(Target value/Test value)*100.00%

Since the blood sample mode can distinguish between whole blood and pre-dilution, and
14
these two different blood sample modes also correspond to different liquid circuit timings,
separate calibration is required for different blood sample modes.
Calibration coefficients are divided into manufacturer calibration coefficients and user
calibration coefficients, corresponding to CBC+DIFF mode. The analysis results will be
calculated by the following formula:

New result=Test value * Factory factor * User factor

Calibration only generates calibration factors and transfer factors for 5 traceable
parameters: WBC, RBC, HGB, MCV, and PLT.

3.3.2 Calibration with calibrator

The calibration of calibration materials with user permissions will obtain the
manufacturer's calibration coefficient and transfer coefficient in one go; The first 5 counts
were conducted in CBC+DIFF analysis mode; After completing 5 counts, new calibration
coefficients and transfer coefficients will be automatically calculated; When exiting this
interface, you will be prompted to save the calibration coefficient.

Before starting calibration, it is necessary to set the batch number, expiration date,
analysis mode, and calibration target value of the calibration material.

Calibration coefficient range [75, 125].

Notice
● Please use designated calibrator within the validity period for calibration.

3.4 GAIN CALIBRATION

Gain calibration includes gain calibration of optical channels, impedance channels and
HGB. The purpose of optical gain calibration is to ensure that the classification algorithm can
accurately classify; the purpose of gain calibration of the impedance channel and HGB is to
ensure that MCV and HGB can calculate the correct values. If it is found that the shape of the
scatter plot is normal but there is no classification result or the classification result is
obviously deviated, you need to check the optical system or recalibrate the optical gain; if you
find that the HGB background voltage on the status interface exceeds the range, you need to
recalibrate the HGB gain.
The gain calibration interface can calibrate optical gain, MCV gain and HGB gain at the
same time. The optical gain is a software gain, which is calibrated using the mean method.
You can decide whether the counting result participates in the mean calculation by clicking
the "check" box of a single count; the MCV gain and HGB gain are hardware gains, which are
calibrated using the successive approximation method. The "checked" box has no effect on
the results of MCV gain and HGB gain.
The gain calibration results are colored to distinguish whether they are successful or not.
If not, the result will be displayed in red and the gain calibration needs to be redone.
If the target value is not set, the corresponding gain coefficient will not be calibrated.
Exiting the gain calibration interface will prompt whether to save the gain coefficient.

Notice

15
● The gain calibration interface only supports calibration using calibrators;
● Please use designated calibrator within the valid period for gain calibration;
● Please refer to the calibration target value sheet for the target value.

3.5 SOFTWARE INSTALLATION AND UPGRADATION

3.5.1 Copy the unziped file“update”to a formatted U disk（FAT32 format）;

3.5.2 Insert this U disk on the machine ,and then power on this
machine,then this machine will turn to the upgradation interface automatically;
3.5.3 Click the upgrade button, then upgrade it according to the upgradation
guidelines;
3.5.4 After upgradation ,machine will enter to the maininterface
automatically;

Notice
● Do not remove the USB disk or cut off the power during tdhe upgrade process, as this
may cause the instrument to fail to start.
● If the upgrade prompt fails, you can try to upgrade again.

3.6 STATUS INDICATOR

The current status of the system is displayed through the three-color indicator lights on
the panel door; all flashes are: one flash cycle every 2 seconds; the indicator lights change
with the change of the instrument status, see the table below:

Instrument Status Indicator Meanings

Ready Steady green Allow to execute sequentialoperations
Working Flashing green Executing sequential operation
Working with fault Flashing Red Working with fault
Not working with fault Steady Red Not working with fault
Can not excute anything Steady yellow During startup or hibernation state
without fault
Enter/exit sleep mode Flashing yellow Enter/exit sleep mode

16
 Chapter 4 Hardware system

4.1 INTRODUCTION OF HARDWARE

The hardware system of the automatic hematology analyzer consists of four parts: (1)main
board; (2)signal board;(3) driver board;(4)optical module, in addition to the board room and board
and component interconnection cables.

1、10.4 inches color display\LVDS interface\resolution

800×600\Appearance236mm×177mm
Displays resources
2、10.4 Resistive touch screen
1、TCP/IP internet interface

Interface resources 2、4*USB ports

3、Serial communication interface
1、It can meet the drive of four-way two-phase stepper motor
Motor driver 2、Able to meet 16
resources subdivision designs
3、The peak drive output current can reach 2.2A
1、It can meet the temperature acquisition requirements
Input and output
sensor resources 2、It can realize positive and negative pressure measurement
3、Photoelectric sensor signals can be read correctly
4、Able to read 1D barcodes accurately
Signal Processing 1、Accurate reading of conversion optical WBC signals
Design Resource 2、It can accurately read the RBC/PLT signal of the turn
counting cell
3、Accurate reading of HGB to HGB signals

4.2 MAIN CONTROL BOARD MODULE

4.2.1 The main input signal of the motherboard

1. USB2.0, network port 100M (IEEE 802.3);

2. Signal board data input (TTL signal);
3. Barcode scanning signal input (serial port signal);
4. Feedback signal of the motion state of the driver board (serial port signal);
5. SD card signal input (SD card 2.0);
6. Serial communication with PC (serial port);
7. Resistive touch screen signal input (analog signal).
17
4.2.2 The main output signal of the motherboard

1. Display data output (LVDS signal);

2. Send instructions to the driver board (serial port signal);
3. Barcode scanning control signal (effective at low level);
4. Printer data signal (serial port signal);
5. Network port 100M (IEEE 802.3);
6. Serial communication with PC (serial port).

4.2.3 Functionality and performance implementation

Main control board function module (Figure 1)

4.2.4 Introduction to the circuit module

1、Screen display function and touch operation design scheme

Display function module (Figure 2)

As shown in the figure above, since the screen we use is an LVDS signal and the CPU output is
an RGB signal, we use THC63LVDM83D cores here.

18
 The touch screen outputs as an analog signal, which is divided into X and Y directions, and this
analog signal is converted to the digital signal by the CPU's internal AD, the algorithm process, and
the processing result determines the position of the touch, so as to realize the touch function.

2、Network port communication design scheme

Network port function module (Figure 3)

The AR8035 is used to convert the RGMII signal to the MDI signal and transmit data to 100M.

3、Communication design scheme between CPU and signal board

Interface function module (Figure 4)

As shown in the figure above, the FPGA uploads the signal data to the CPU, and the CPU
algorithmizes the signal data, and the communication between them is 12-bit parallel communication,
and the transmission speed is fast, up to 30M

4、The communication design scheme between the CPU and each serial port

Interface function module (Figure 5)

19
4.2.5 Common faults and analysis

The common fault phenomena and solutions of the main control board are limited to the
hardware perspective, excluding software failures that cause the same fault phenomena, but many
faults need to be detected by software.

Before troubleshooting MCU-related faults, check the following points:

1、Whether the wires connected to the main control board are loose and whether there is
unreliable connection;
2、Whether the tag of the cable identification corresponds to the tag of the socket on the main
control board, and whether the cable is broken or damaged;
3、Whether the power supply input of board socket J1 is normal (the multimeter tests whether
the voltage between the black wire and the red wire is positive 5V);

After confirming that the cable connection, input power supply and indicator light are normal,
check the fault as follows:
20
Fault phenomenon Solution

1. Make sure that the input mains power is

normal, and if it is normal, enter the next step to
check;

2. Check whether the voltage on each voltage

test point on the motherboard is normal, and
check whether the board is short-circuited if not;

3. Check whether the backlight is lit, and if not,

whether the high-voltage strip is damaged;
1、It can't be turned on
4. Check whether the connection line between
the main control board and the backlight interface
and the LCD screen is reliably connected, plug
and unplug it again, and if the fault disappears
after re-powering on, it is an unreliable fault of
the cable connection, otherwise proceed to the
next step;

5. Replace the LCD screen, if the fault

disappears, it is the LCD screen component
failure, otherwise proceed to the next step.
1. Check whether the SD card is securely plugged
in.

2、The boot kernel cannot be loaded 2. Check that the connector of the motherboard
and the core board is soldered or the lead angle
and pad fall off.

1. Check whether the barcode type is the type

supported by the barcoder

3. Check whether the scanner connection line

3、Unable to read barcode information has poor contact or breakage;

3. If the above faults have been eliminated, please

replace the scanner parts.
1. Check whether the printer line has poor contact
or breakage;
4、The test results cannot be printed
2. If the above faults have been eliminated, please
replace the printer parts.
1. After the power is turned off, in the case of live
5、The clock was reset every time when turning power, use a multimeter to test the voltage at both
on the machine ends of the battery clamp, and the voltage at both
ends < 2.8V, it is a fault for insufficient battery
power and replace the battery.

21
 When disassembling the board, please wear anti-static gloves;
When removing the board, make sure to turn off the power supply and unplug the power cord.

Installation:
Follow the steps for disassembly in reverse.
Verification：
1、Check that the board screws are fully installed;
2、Connect the power cord and turn on the AC control switch, the machine enters the
initialization state, and various indicators on the board light up.

Ensure that the board and the host chassis are fastened and connected by screws;
The removal operation can only be performed after the board and its surroundings have cooled.

4.3 SIGNAL BOARD MODULE

4.3.1 The main input signal of the signal board

Optical high-angle (HS), medium-angle (MS), low-angle (LS) signals, these three input signals
come from the output of the optical signal amplifier board, they are pulse signals, their periods are the
same and T=2.5us, amplitude V(HS)=1.2V, V(MS)=1V, V(LS)=0.8V (the above is an approximate
value);
Red blood cells (RBCs), which are derived from the RBC counting cell, are pulsed signals,
period T=16us, amplitude V=1mv (this is approximate);
The hemoglobin (HGB) signal, which is an input signal from the HGB measuring cup, is a DC
signal with an amplitude of approximately 1.5V.

4.3.2 The main output signal of the signal board

Signal board signal output: FPGA collects data for preliminary sorting and uploads it to the CPU
(main control board AM3358), and the FPGA communicates with the CPU as a 12-bit parallel
communication protocol.

4.3.3 Functionality and performance implementation

22
 Signal function module（Figure 6）

4.3.4 Introduction to the module circuit

HS、MS、LS Acquisition function module（Figure 7）

HS, MS, LS are pulsed signals, the period is the same and T=2.5us, the amplitude V(HS)=1.2V,
V(MS)=1V, V(LS)=0.8V, the value amplified by the AD8034 is V(HS)=3.6V, V(MS)=3V,
V(LS)=2.4V (the above specific magnification will be fine-tuned according to the target value, so the
above is an approximate value), the AD9224 converts these analog signals into digital signals for
FPGA acquisition and upload CPU.

RBC Acquisition function module（Figure 8）

RBC is a pulse signal, the period T=16us, the input amplitude V=1mv, the amplitude is about
0.9V after 921 times amplification, AD9224 converts this signal into a digital signal, for FPGA
acquisition and upload to the CPU, after 921 times amplification of the RBC signal amplified by 4
times to obtain a PLT signal, this PLT is also a pulse signal, period T=16us, the amplitude is about
3.6V, after the AD9221 converts it into a digital signal for FPGA acquisition and then uploads to CPU.

23
 HGB Acquisition function module（Figure 9）

The AD7265 converts the HGB signal to a DC signal with an input amplitude of approximately
1.5mV, which is amplified by 1000x and converted to a digital signal that can be captured by the
FPGA and uploaded to the CPU

24
4.3.5 Common faults and analysis

The signal board circuit completes the conditioning and amplification of WBC, RBC/PLT and
HGB signals, so that the signals before entering A/D are basically real and suitable for A/D conversion
signals; The A/D module is the interface of the analog circuit and the digital circuit module, which
completes the sampling of the above sensing signal and other monitoring signals, and converts the
analog signal into the digital signal suitable for the digital circuit processing.

25
Fault phenomenon Solution:

1. There is no response 1. Check whether the suction key connection wire is loose or broken, if so,
when the sample please plug and unplug or replace the wire;
aspiration button is 2. If the fault cannot be eliminated from 1, remove the connecting plate of
pressed the sample suction key switch to see if the switch is splashed, if so, wipe
the liquid clean, and reinstall it.
2. No WBC value is 1. Replace the motherboard to confirm that there is no fault in other
measured components
3. No RBC value is 1. Replace the motherboard to confirm that there is no fault in other
measured components
4. Measurement 1. Replace the motherboard to confirm that there is no fault in other
without HGB value components

4.4 Driver board module

4.4.1 The main input signal of the driver board

1、Command issuance of the main control board (serial port signal);

2、Motor position signal feedback (photoelectric sensor signal);
3、Hydraulic pressure and negative pressure pump pressure signal feedback;
4、Temperature sensor signal input.

4.4.2 The main output signal of the driver board

1、The feedback of the status of the motion mechanism is sent to the main control board (serial
port signal);
2、Control signals for valves;
3、Stepper motor control signal;
4、Liquid heating control signal for pipelines.

4.4.3 Functionality and performance implementation

Driver board function module (Figure 10)

26
4.4.4 Introduction to the module circuit

1、Stepper motor drive design

Stepping motor function module（Figure 11）

The drive of the stepper motor requires two parts of the circuit, one is the driver chip, the other is connected to
the optocoupler control signal, and the control signal is converted into the rotation direction and microstep number
of the motor, and the FPGA outputs two signals: direction, step pulse: clock; The stepper motor is driven at 24V The
current is determined by the voltage at the VREF terminal of the driver chip.

2、Drive of pumps, valves

Pump, valve function module (Figure 12)

The principle of pump and valve drive is to control the switch of the CMOS tube circuit by the high and low
level of the FPGA output, and each pump and valve corresponds to a CMOS tube circuit.

3、Drive of trough type dust-proof snap type light control switch

27
 Slot sensor function module(Figure 12)
The fork sensor works by passing through the groove notch of the horse plate to sense the step position of the
motor, and when the fork sensor is blocked by light, the output is high, and then the signal is transmitted to the FPGA
through the logic circuit.

4.4.5 Common faults and analysis

Before troubleshooting drive-related faults, first check whether the cable connected to the driver board is loose,
whether there is unreliable connection, whether the mark of the cable identification corresponds to the number of the
socket on the simulated driver board, and whether the cable is broken or damaged.
If you suspect that the analog driver board is faulty, first check whether the indicator lights of the driver board
are normal.
After confirming that all the indicators of the driver board are normal, it is necessary to self-check whether the
solenoid valve and moving parts have reported the corresponding faults through the "self-test" function of the
software interface.

No The type of failure Fault description Troubleshooting and solutions

1、First, check whether the power supply of the
board is normal.
2、Check whether the connection between the motor
and the optocoupler wire in the corresponding
channel is reliable, whether the joints at both ends
are well connected, whether the identification of the
optocoupler and motor connection wire corresponds
to its position, and whether the wire is broken or
damaged;
a、The motor does not work; 3、After confirming 1 and 2, perform maintenance
Motors and b、Motor action but report operations under the debugging interface of the host
1
optocouplers motor failure or optocoupler computer to control the corresponding fault points
failure: in a single way to see if the fault can be eliminated;
4、Check whether the optocoupler of the
corresponding channel has dust blocking the
light-emitting surface, or liquid splashes on the
light-emitting surface of the optocoupler, if so, wipe
the optocoupler surface clean and reinstall it to see
whether the fault is eliminated, if not, replace the
optocoupler;
5、If the fault cannot be eliminated by replacing the
28
 optocoupler, replace the driver board to see if the
fault can be eliminated.
6 、If the fault cannot be eliminated, replace the
corresponding channel motor to see if the fault is
eliminated;
7、If the fault still cannot be eliminated, it should be
suspected that the mechanical component failure is
caused (such as too much friction), please follow
the mechanical troubleshooting.

1、First of all, check whether the motor connection

wire of the corresponding channel is loose, broken
or damaged, if the above phenomena exist, please
re-plug the wire or replace the wire in the case of
power failure;
2、Check whether the fastening screws of the motor
and optocoupler mechanical components are loose,
Abnormal motor noise if the above phenomena exist, please tighten the
screws;
3、If 1 and 2 can be ruled out, the driver board may
be suspected to be faulty, please replace the analog
driver board;
4、If the fault cannot be eliminated by 3, the motor
assembly needs to be replaced.

1、First of all, perform maintenance operations

under the debugging interface of the host computer,
whether the corresponding valve can be switched on
and off normally (under normal circumstances, the
switch valve can hear the sound of "pop"), if so,
there is no fault in the valve drive, please check the
fault from the hydraulic circuit;
2、If the valve cannot be opened and closed
normally, please check whether the valve
connection wire is loose or the connection is
unreliable or whether there is a break, if the above
situation exists, please re-plug or replace the valve
connection wire;
Suspect the valve is not 3、If the fault cannot be eliminated through 2, then
2 Valves working other valve connection wires can be connected to
the valve, and then the operation through the
maintenance interface operation to confirm whether
the valve starting circuit is faulty or there is a
problem with the valve itself (if it is suspected that
there is a problem with valve 2, you can connect
valve 3 to the line to valve 2, and then operate to
open and close valve 3 in the maintenance interface,
if the valve can be switched on and off normally, it
means that the analog drive board has been
damaged and needs to be replaced, if the valve
cannot be switched on and off normally, it means
that valve 2 is broken and the valve needs to be
replaced)
1. First of all, check whether the vacuum pump can
work normally through the operation of the
a、Report abnormal pressure maintenance interface, and if so, the fault should be
3 Pumps checked from the gas circuit;
b、The pump does not work
2. If the pump cannot work normally, please check
whether the pump connection line is loose or

29
 unreliable, whether there is any breakage, if the
above situation exists, please plug and unplug or
replace the pump connection line;
3. If the fault cannot be eliminated through 2,
replace the analog driver board to see if the fault
can be eliminated;
4. If the fault cannot be eliminated, the
corresponding pump needs to be replaced.

1. Check whether the working indicators of the

main control board and driver board are normal, if
not, replace the corresponding board;
2. Check whether the connection line between the
main control board and the driver board is loose,
Report a communication and if it is loose, how to re-plug it;
4 Communication failure 3. If the fault cannot be eliminated, replace the
connection line between the main control board and
the driver board;
4. If the fault still exists, the driver board and the
main control board should be replaced successively,
and the fault can be basically eliminated.
1. Check whether the connection cable of the liquid
level sensor connector is loose, splashed or broken,
Liquid level False alarm of liquid level if so, please re-plug and unplug the connector, or
5 sensors status wipe the liquid clean and plug it back in, or replace
the waste liquid connector cable.
2. If not, the liquid level sensor assembly should be
changed.

30
 When disassembling the board, please wear anti-static gloves;
When removing the board, make sure to turn off the power supply and unplug the power cord.
Installation:
Follow the steps for disassembly in reverse.
Verification:
1. Check whether the board screws have been fully installed;
2、Connect the power cord and turn on the AC control switch, the machine enters the initialization state,
and various indicators on the board light up.

The connection between the board and the host chassis must be tightened by screws.
The removal operation can only be performed after the board and its surroundings have cooled.

4.5 WBC Optical Signal Amplification Board Module

4.5.1 Functionality and performance implementation

Signal function module（Figure 13）

4.5.2 Introduction to the module circuit

Current-to-voltage function module (Fig. 14)

The main is to convert the WBC three-way photoelectric signal into a stable voltage signal.

31
4.5.3 Introduction to board interfaces

4.6 Laser driver board module

4.6.1 Functionality and performance implementation

When the laser diode flows through the current above the threshold, the laser will be generated, and the light
output will be affected when the temperature changes, and the constant current reverse design must be adopted to
ensure that the output of the laser diode is not affected by the temperature.

Laser-driven function modules（Figure 15）

Because the current of the PD is proportional to the output of the light output, as long as the PD current is
constant, the light output is also a constant value, and the laser is injected into the photodiode PD, the PD generates

32
the output current and converts the current into a feedback voltage with a resistor, which is equal to the reference
voltage to control the forward current of the laser diode, and a stable light output is obtained.

4.6.2 Introduction to board interfaces

4.7 High-angle PD board module

4.7.1 The main input signal of the high-angle PD board

The input signal of this plate: the light signal scattered at a 45-degree angle of the flow chamber, the
characteristics of this light signal: when the flow chamber has blood cells passing through, this light will be
relatively stronger, when the blood cells pass through, this light will be relatively weaker.

4.7.2 The main output signal of the high-angle PD board

The output signal of this board is a pulse signal, which is characterized by an average period of 2.5us and an
amplitude of about a few mV.

4.7.3 Functionality and performance implementation

S-25C is the image sensor, model: S-25C, brand: American OSI. After the laser passes through the flow
chamber, the scattered light at an angle of 45 degrees to the laser hits the image sensor S-25C, when the flow
chamber is a uniform dilution (that is, there are no blood cells), then the light hitting the S-25C is uniform, then the

33
 output of the S-25C is a few mV of direct current, when there are blood cells flowing through the flow
chamber, then the light hitting the S-25C. The intensity will become stronger, and the S-25C will output a forward
pulse signal of a few mV, which has a period of 2.5us and an amplitude of several mV.

34
 Chapter 5 Fault alarm information
Involved Fault Repair Guide
Associated Potential
Faults fault triggering Device Troubleshooting and
features failure modes
coding mechanism interface maintenance process
The For occasional failures:
The serial
Analog driver communicatio 1. Check the connection
communication
board n module is between the digital board J81
protocol is
damaged and the analog driver J31
wrong
The For betfair failures:
No header,
Communic Main control communicatio 1，Check the connection
0x0100 ultra-long
ation board n module is / between the main control
001 frame,
failures damaged board J81 and the analog
insufficient
The main control driver J31
header, wrong
board J81 is 2，Replace the associated
check code, and The wire is
connected to the board
unrecognized loose
analog driver
command word
board J31
1， Check whether the
parameters under the voltage
and current status interface
are within the normal range
2，If it is within the normal
Main control range, replace the main
board control board
The main
The AD range is Analog driver Voltage and 3， Otherwise, confirm
control 0x0100
more board / current status whether the power board and
board is 002
than(2.44-2.55） Power board and interface its connecting cables, and
faulty
its connecting whether the connection
cables between the analog driver
board J2 and the main control
board J86 are normal
4，If all the above checks are
normal, replace the analog
driver board
1，Check whether the digital
board coin cell battery is
installed
2，If installed, replace the
Check that the new button cell battery, reset
The system Coin cell batteries
0x0100 system clock is the date and time in the
clock is Main control /
004 less than setting interface, save and
faulty board
January 1, 2000 exit and then shut down and
restart
3，If the fault persists after
startup, replace the main
control board
1，Confirm that there is no
1，The diluent alarm prompt on the reagent
Bubbles
residue is less management interface
than 4% 2，Confirm that the
2，There are no Reagent remaining amount of reagent
The diluent 0x0100 Diluent associated
reagents or management in the diluent bucket is
runs out 0104 tubing
abnormalities Air leakage interface sufficient
that cause from pipes & 3，Check that the pipeline
insufficient valves outside the machine is not
reagent addition broken or leaking
4，Disassemble the housing
35
 of the whole machine and
check the related pipelines
(cap assembly, valves,
electromagnetic dosing
pumps, pipeline joints, etc.)
1，The 1，Confirm that there is no
remaining alarm prompt on the reagent
amount of LH management interface
1，The
Lyse is less than 2，Confirm that the reagent
quantitative
10% balance in the reagent bucket
LH Lyse is 0x0100 pump fails
2，There are no is sufficient
exhausted 0105 2，Lyse
reagents or 3，Check that the pipeline
related
abnormalities outside the machine is not
pipelines,
that cause broken or leaking
1,Lyse reagent valve leakage,
insufficient Reagent 4，Disassemble the shell of
related pipelines and death
reagent addition management the whole machine and check
2,Anti-soluble 3，Non-hemol
1，Diff interface the associated pipelines (cap
samples ysis due to
hemolytic agent assembly, valve,
other reasons,
balance is less electromagnetic quantitative
such as
than 3% pump, pipeline joint, etc.)
Diff Lyse non-claimed
0x0100 2，There are no 5，Use quality control and
is quality control,
0106 reagents or calibrators that are not
exhausted use of
abnormalities claimed by the company
calibrators
that cause
insufficient
reagent addition
There is a 1，Confirm whether the
foreign object indication information on the
on the float, sensor status interface meets
which causes expectations
the state to 2，Confirm that the liquid
The waste
0x0100 Liquid Waste change Sensor status waste sensor cable is properly
liquid is Float state
0110 Sensor Assembly properly screen connected
full
Waste liquid 3，Whether the float can
sensor change state normally
The BNC head
is not
connected
56V The power 1，Confirm whether the
0x0100
Overrange(47,6 Power board board is indication information on the
0201
0) damaged voltage and current status
The power interface meets expectations
12V
0x0100 cord is not 2，According to the interface
Over-Range Power cord
0202 securely instructions, confirm whether
(11.5, 12.5)
connected the voltage of the relevant
Negative 12V power board test port meets
0x0100 Analog driver The board is
out of range the expectation
0203 board damaged Voltage and
Power (-12.5, -11.5) 3，Confirm whether the
current status
failure P12V Out of power cable connection from
0x0100 Peripherals and interfaces
Range (11.5, Short circuit the power board to the analog
0204 their cables
12.5) driver board is normal
4，Confirm that there is no
short circuit between the
P24V Out of analog peripherals and the
0x0100
Range (22.0, / / power drive peripherals and
0205
29.0) their connecting wires
5，Replace the analog driver
board

36
 The warm-up Warmed pool Temperature 1，Check the temperature
pool components sensor value corresponding to the
The shaft
temperature status interface
temperatur 0x0100
read from the 2，First, confirm whether the
e sensor is 0401 Temperature The wire is
analog driver ambient temperature is in an
faulty sensor cable loose
board is either 0 over-temperature state
or 70 3，If the temperature value is
Temperature not "0", it means that the
The optical Optical module Temperature temperature detection sensor
Failure of sensor
temperature & Pressure is short-circuited, and the
the optical read from the
0x0100 Status corresponding components
component analog driver Temperature The wire falls
0402 Interface are replaced’
temperatur board is 0 or 70 sensor off
e sensor 4，If the temperature value is
"70", the temperature
Diluent
The optical The cable is detection sensor is
temperatur
temperature detached or the disconnected, first check
e sensor 0x0100 Temperature
read from the temperature whether there is an unreliable
malfunctio 0403 sensor cable
analog driver sensor is line connection, and then
n
board is 0 or 70 damaged replace the corresponding
components
Failure of The heating 1，Enter the interface of
the When the rod is damaged temperature and pressure, if
preheating machine is The the temperature of the
component turned on, the temperature pre-heating pool is lower than
temperature of Warmed pool sensor is Temperature 35 degrees Celsius or higher
0x0100 the preheating components damaged & Pressure than 55 degrees Celsius, the
0404 pool rises by The Status component failure can be
less than 1 temperature Interface judged
degree for 3 switch is 2，If there is no
consecutive damaged improvement in re-inserting
minutes Component-relate The wire falls the cable, replace the
d cables off pre-heating module
First, check whether the laser
The laser is current is abnormal on the
Optical module
damaged status screen
Laser driver The board is Open the optical system
board damaged shielding box, manually
The shield box switch the shielding box
Optical module switch is switch, if you can't see the
damaged laser lighting, then check
whether the optical system
connection line, shielding
box switch, laser connection
line and other connections are
reliable; if there is no
Laser tube Voltage and connection problem, refer to
Laser 0x0100
current out of current status the hardware troubleshooting
failure 0809
range (20,70) interfaces steps to confirm whether the
laser driver board and the
analog driver board are
Optical system The wire falls normal
related cables off If the laser can be lit
normally, refer to the
hardware troubleshooting
steps to confirm whether the
laser driver board is normal,
otherwise replace the optical
module
If the laser itself is damaged,
the optical system needs to be
replaced directly
37
 The Enter the system self-test
optocoupler is interface to confirm the
Optocoupler damaged or problem
the surface is Refer to the fault code to
/ dirty check the wire, optocoupler,
The motor is motor, and motion
Syringe Syringe motor
damaged interference
assembly
Related The wire is The system
failure
connection cables loose is restricted
Mechanical
interference or
Transmission
other causes
mechanism
cause limited
movement
The lifting Vertical position Enter the system self-test
/
mechanism optocoupler interface to confirm the
of the Lifting motors / problem
sampling Limit occurs The system Refer to the fault code to
assembly Mechanical limits during is restricted check the wire, optocoupler,
is faulty movement motor, and motion
Related The wire is interference
connection cables loose
The Deflection Enter the system self-test
rotating position / interface to confirm the
mechanism optocoupler problem
of the Refer to the fault code to
sampling Deflection motors / check the wire, optocoupler,
assembly The system motor, and motion
is faulty is restricted interference
Interference
Mechanical limits occurs during
movement
Related The wire is
connection cables loose
The reagent is Confirm the quality of the
Reagents contaminated reagent
or expired If the background PLT is
Abnormal found to be high, confirm the
shutdown impedance channel shielding
(refer to the impedance
The boot
Backgroun channel signal interference
0x0100 background is
d alarm processing)
0702 not up to
anomalies Pipelines for the Confirm the cleanliness of
standard Poor
whole machine the counting pool, etc., and
maintenance
guide the maintenance
habits
habits .
Confirm that there is no air
leakage in the pipeline
connection and valve
There is a Confirm that there are no
WBC Reaction
foreign body foreign substances in the
Cell
in the pool WBC reaction cell
The HGB gain Confirm that the reagent
The Setup of the
is not set perfusion in the pool is
backgroun HGB voltage whole machine Voltage and
0x0100 properly normal
d voltage out of range current status
0801 Enter the voltage and current
of HGB is (3.2, 4.9) interface
status interface to confirm the
abnormal
HGB module HGB background voltage
HGB module
is damaged Confirm the HGB gain
setting
Check the HGB components

38
 The associated Check whether the hydraulic
line is broken, pressure value under the
Hydraulic sensor
and the valve status interface is close to the
associated lines
is clogged or current local air pressure
fails Check whether the pipeline
According to
The cable is Temperature of the whole machine is
The liquid MCV, the Hydraulic sensors
0x0100 loose or the & Pressure broken, the valve is blocked
pressure is absolute and their
0803 sensor is Status or damaged
out of limit pressure connecting cables
damaged Interface Check whether the sampling
exceeds 320Kpa
needle and optical system are
blocked
Severe
Optical module Check the hydraulic sensor
blockage
and its connecting wires for
looseness
Enter the interface between
Liquid waste Dirty, blocked, temperature and pressure to
pumps ineffective observe whether the pressure
building process is normal
(such as whether the waste
liquid pump is operating)
Observe whether the pressure
Associated Dirty, blocked,
under the interface can be
solenoid valves ineffective
maintained, if not, check
The vacuum
The whether there is air leakage
pressure is
vacuum 0x0100 Temperature in the relevant pipelines and
outside the
pressure is 0804 & Pressure valves
range specified
abnormal Related pipelines Air leakage Status If it cannot be established,
by the timing
Interface check whether the waste
liquid pump fails or the
pipeline leaks
If the waste liquid pump does
not work, it is necessary to
Analog driver The board is
check whether the relevant
board damaged
wire connection and the
simulated driver board are
normal
Check whether the
temperature of the optical
system under the status
interface meets the
The temperature The cable is expectation, and judge
Temperature
The of the loose or the whether the state is abnormal
Temperature & Pressure
temperatur preheating pool temperature due to the replacement of the
sensor cable Status
e of the 0x0100 is out of range sensor is diluent with too large
Interface
preheating 0805 (target damaged temperature difference
pool is temperature (recoverable, non-faulty)
abnormal -1.5, target See Temperature Sensor
temperature +3) Failure to Resolve Sensor
Issues
Pre-heating
See Shaft Component Failure
components
The fan is Check whether the
The Cooling fan damaged or temperature of the optical
temperatur The optical module the cable is Temperature system under the status
e of the 0x0100 temperature loose & Pressure interface meets the
whole 0806 exceeds 40 Status expectation
The air inlet and The air inlet Check whether the fan
machine is degrees Celsius Interface
outlet of the and outlet are assembly is working (the fan
too high
whole machine blocked assembly is bad or the

39
 The simulated driver board is
temperature damaged), and the colleague
sensor is checks whether the air outlet
Optical module damaged or and air inlet are blocked
the associated See Temperature Sensor
cables are Failure to Resolve Sensor
loose Issues
Analog driver The board is
board damaged
The fan is Check that the temperature of
Cooling fan damaged or the diluent in the status
module the cable is interface is as expected
loose Check whether the fan
The room assembly is working, and
Ambient
The diluent temperature is check whether the air outlet
The temperature temperature Temperature
temperatur too high and air inlet are blocked
0x0100 of the dilution & Pressure Refer to Temperature Sensor
e is beyond
080C exceeds 40 The Status Failure to Solve Sensor
the upper
degrees Celsius temperature Interface Problems
limit
sensor is Confirm that the ambient
Temperature
damaged or temperature is within the
sensor cable
the associated declared operating
cables are temperature of the product
loose

The gem hole /

The orifice is clogged or
0x0100 voltage is the connection System
Blockage RBC chamber
0901 ultra-high (24V) between the Self-test
or abrupt front and back
pools fails
Gemstone The system self-test interface
Gem hole holes of the runs the hole voltage
wrong size detection
If the gem hole is replaced, it
Bore voltage is necessary to check whether
The
The orifice less than 17V the gem hole specification is
0x0100 temperature
voltage is (after Self-test wrong, and whether there is a
0902 Diluent sensor is
too low temperature gem hole assembly problem
temperature damaged or
correction) or a crack in the gem hole
sensor assembly the associated
See Diluent Temperature
cables are
Sensor Failure for
loose
troubleshooting temperature
sensor issues
The shield / /
RBC chamber wire is not
tightened
The ground
wire of the
Laboratory
grid is not
Impedance electromagnetic
reliably
channel The PLT noise environment
0x0100 connected to
signal ratio is more
0903 the ground
interferenc than 105
The shield box
e RBC Count Pool
is not
Shielding Box
tightened
The impedance
Analog driver channel shield
board box is not
tightened
40
 Dirt or / Fault 1: Clean the flow
The optical Flow chamber chamber
The sample blockage
system 0x0100 Fault 2: Replace the optical
stream is
signal is 0904 Abnormal system
abnormal Optical module
abnormal front light

41