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Motility Test

The motility test determines the motility of enteric organisms by observing growth patterns in semisolid agar. A positive result is indicated by diffuse growth beyond the inoculation site, while a negative result shows no growth beyond that site. Quality control involves using Proteus vulgaris as a positive control and Klebsiella pneumoniae as a negative control.

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Motility Test

The motility test determines the motility of enteric organisms by observing growth patterns in semisolid agar. A positive result is indicated by diffuse growth beyond the inoculation site, while a negative result shows no growth beyond that site. Quality control involves using Proteus vulgaris as a positive control and Klebsiella pneumoniae as a negative control.

Uploaded by

mindytran95
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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MOTILITY TEST

PURPOSE
Determines whether enteric organisms are motile.
PRINCIPLE
Bacterial motility is evident by a diffuse zone of growth in
semisolid agar extending out from the line of inoculation. Some
organisms grow throughout the entire medium, whereas others
show small areas of growth out from the line of inoculation.
Some tests include triphenyltetrazolium chloride (TTC). TTC in
its oxidized form is colorless. As bacteria grow in the presence
of TTC, it is absorbed and reduced to the insoluble red-colored
pigment formazan.
If TTC is present, growth is indicated by the presence of the red
color, and as motility occurs, small to very large regions of color
can be observed around the area of inoculation. If no TTC is
present, growth is indicated by cloudiness.

METHOD - ABBREVIATED STEPS


1. Touch a straight needle to 1-2 colonies.
2. Stab once 2/3 the depth of the tube in the middle.
3. Incubate tube at 35-37C for 48 hours.
EXPECTED RESULTS
Positive - diffuse growth
beyond site of inoculation
(with or without color)
Negative- no growth beyond
site of inoculation (with out
without color)

QUALITY CONTROL
Positive - Proteus vulgaris
Negative - Klebsiella
pneumoniae

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