0% found this document useful (0 votes)
4 views

chromatography, chem. hon. unit 5

The document discusses the technique of column chromatography, which separates components of a mixture based on their different affinities to a stationary phase and a mobile phase. It outlines the process of separation, the role of the solvent, and the applications of chromatography in biochemical laboratories. Additionally, it mentions the importance of analyzing various substances, including sedatives and metabolites, using this technique.

Uploaded by

uraj44212
Copyright
© © All Rights Reserved
Available Formats
Download as PDF or read online on Scribd
0% found this document useful (0 votes)
4 views

chromatography, chem. hon. unit 5

The document discusses the technique of column chromatography, which separates components of a mixture based on their different affinities to a stationary phase and a mobile phase. It outlines the process of separation, the role of the solvent, and the applications of chromatography in biochemical laboratories. Additionally, it mentions the importance of analyzing various substances, including sedatives and metabolites, using this technique.

Uploaded by

uraj44212
Copyright
© © All Rights Reserved
Available Formats
Download as PDF or read online on Scribd
You are on page 1/ 15
> Column chmynatoyeaphiy ed Chremategraphy in a technique uth wo sect e., Seprate a J bound trom a mixtune a {cd & This _decKniaue 1 ee ae hile” pha ca evtune._th U : the the ay int Phase ag Ad fenenf _vateg- _ walt bow V travel os the great ainsi ned | individual is ake mixture ren adsorption __aud fo Hho Stefanos conten he en Sith he fotionary GAG hee ie = add inity 0 f lane he Components Hoe | mauve a ane Yemeoved gist Ha ft move. I lowly ane elie out fast. , the adsorbtion Solute. Piolecutes to the lolumy occurs fn a | Yevensihle mannen - ‘the rate ot Hie movement of the Components i a Expressed ee : | Re = the. aes by 3 _— fs dlisted a call column Os Loy re aay lattes Povoles remo ing zi ual tp the Solweat Fanaa pres 6 sor BELG i uae _adsovbent _avel dissolve fa tha. eluent - cate A She vo sienanes ies Poem pheoen that transports "fhe ble Compo ee ee The __mixt ae Soluent thet ids a Sie . is Known aD an elfte - The 2 lutte. in made up ‘of Be tis) mabile phave, aurol anabires. A__sulstauco that seprates aud >| move i fpf a mictiune : | thorough the Colpemn of a lecematesraph- The dluzent tn Se ——— Shoeld __medth - The —mobile |shase | ov ehuend 1s atlben | Solent on a _mix tune a Solvent - fin tse first | Step ti end —_mixtine phot needs _-tn __be Sefrratecl _. in added |) Tie column + hee the ee an 1% bre mont + fe zs T then thety orden based a || bolanity= tl{ be aa follouss Blue S wed is Geren i vi J an ee | As’ ie. polanit io: ogee a i - ‘ t sapoud ts Fe bh ot lf move | s When it onrives at te, 3 ! af Ma (eluren ie ip e _ = — Collector} + tn 9 Clea test hae ——__| Atten “this ; eee roe Ss ff collected gud | fempbourd fp AE a these ane colluted fn ri ; tales ae —Sepracte. a Abplialions of Column chromadorve bs, oe eh ae a pa Calg ly F chromategraphy — technig n___ Shrac ts 5s ae eee TPN ugh which a Solution _ [ a opie | fo. Called p th . ee Har mobile = | te Seprat-ion “a so __|| takes Be : an [tee misclune Sebrale | Dee ney oa er cliff entice 1 ae = | inity _bowands "" shite affinity onany aud || mobile” _phare Solent 7 | ieee iUony actin Oren, Pe 1 Ailcertgs Ms daas a . Ligiricl en h By tt do ected based toni! tin i complexity of His Solwenk » paper | | méixttene “, etc. Usecall antending | bythe oy vadial p Son chrom atoyre pp iS vsecl ap they! “ane ae: Also it in eaot ty han ese) _chromatoyam —_obteined is sfacten cul Hass __prmess is __fess time _conserninsgs 4 Saal cried —lelendtify Le sample __{ | chem atesicinns aha, To examine the yeh eee biochemical laberatrvies. Gol 2 mixtunes:' 4 She _expenimeat f& concurted ona Shi 2 E luminion foil. _ Plastic or glass dich Lut. Sf __@ || the —_Seponation nelative agginey of Beno fowands cay thu" bhanen = I Cembournals ia ctr mobile Over tle Surface of phases The movement Occurs fre puch & H_G_tvtay oa Haw compound g hich ee 2ffinity to tha — pha move. solowalas while other tombounds travel Udon. Bo sae, rise te teraction ef ct Sau sla ee is @tfeaned - ‘On Corplion hie sefrration foc fhe aE id att TE Plate peat 7 Procedune SS > Difkonent (ombaneasts required _ke BoA Ha -brecechune along wito He phones Croton os oer ce cb a @ Thin layer chromatography plake os rrachy . ihc ie ie ae nent aud stable - The —— i plied on ifs sunfaco— ——— oy = Cx boaiimenbs ee he fhe -pitsloray I is “i _to ae { to Dad mos. [2 Apply Sample | sea pote thin ora | Mode “nt _ i es el beds | ith ta Ore fa: | Albly Sonite Sluts Ja Hur tanked Spa: Q i. [* four the mobile phase indo Ha Tie i ied tt into ‘di: i fe ! i. 1 Place the plate tn Ha. TL chambon auc) : 1: out any [Such Ove thal the Soombl Sacer. wo thes mobile! panes : a Lampe ate talor Wleralay | ty Keep oe spots Deel cxaliog immense ib fn He » Wait HU Ha ee ence the Shotz ane Losel of He. mobile phase - Bo not, Ha. plates aud dary th The Soumbe Spa det a i + The qualitative testing | oni ade cines f “uch as Sedatives , Local anoesthetica 1 onsiconu= | -ulsant freunqerilisers » analgesion—s a Sihistarsin or | _Stenaid > hybnotice & —clone_by — | -Tle. b extremely urefull | analysis duh ar _Sepanaction or | oh “biochemical metabo bites frac ite_bloot plasma» Unine » body flustd + Serum etc: eA

You might also like