The document discusses the technique of column chromatography, which separates components of a mixture based on their different affinities to a stationary phase and a mobile phase. It outlines the process of separation, the role of the solvent, and the applications of chromatography in biochemical laboratories. Additionally, it mentions the importance of analyzing various substances, including sedatives and metabolites, using this technique.
The document discusses the technique of column chromatography, which separates components of a mixture based on their different affinities to a stationary phase and a mobile phase. It outlines the process of separation, the role of the solvent, and the applications of chromatography in biochemical laboratories. Additionally, it mentions the importance of analyzing various substances, including sedatives and metabolites, using this technique.
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