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HPLC Application 18229 Pharma ABN Test 12 Mix On Luna 5um C18 (2) 150x4.6mm 1ml-Min

The document outlines the LC conditions for testing a mix of pharmaceuticals using a Luna 5 µm C18(2) column. It details the mobile phase composition, gradient profile, flow rate, temperature, and detection method. Additionally, it includes a note about the SecurityGuard™ Guard Cartridge System to extend column lifetime.

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11 views1 page

HPLC Application 18229 Pharma ABN Test 12 Mix On Luna 5um C18 (2) 150x4.6mm 1ml-Min

The document outlines the LC conditions for testing a mix of pharmaceuticals using a Luna 5 µm C18(2) column. It details the mobile phase composition, gradient profile, flow rate, temperature, and detection method. Additionally, it includes a note about the SecurityGuard™ Guard Cartridge System to extend column lifetime.

Uploaded by

cianrango
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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2/4/25, 9:15 AM about:blank

App ID 18229

Pharma ABN Test 12 Mix on Luna 5um C18(2) 150x4.6mm 1mL/min


Order Items Used in This
Application

00F-4252-E0
Luna 5 µm C18(2) 100 Å, LC Column 150 x 4.6
mm, Ea

LC Conditions 1. Pyridine
Column: Luna 5 µm C18(2) 100 Å, LC Column 150 x
4.6 mm, Ea 2. Acetaminophen

Brand Name: Luna 3. Quinine


Part No: 00F-4252-E0
4. Acebutolol
Phase Name: C18(2)
5. Chlorpheniramine
Elution Type: Gradient
Mobile Phase: A: water w/ 0.1% formic acid 6. Triprolidine
B: acetonitrile w/ 0.1% formic acid
7. Prednisolone
Gradient Profile: Step No. Time(min) %A %B
1 0 95 5 8. 4-Chlorobenzoic acid
2 1.67 95 5
9. 4-Chlorocinnamic acid
3 21.69 5 95
4 21.83 95 5 10. Diazepam

5 30.56 95 5 11. Diflunisal

Flow Rate: 1 mL/min 12. Hexanophenone


Temperature: 45°C
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 254 nm
(25°C)
Sample Note: SecurityGuard™ Guard Cartridge System
extends column lifetime. - SecurityGuard
Cartridges, C18 4 x 3.0mm, 10/Pk Part No.:
AJ0-4287 - Holder Part No.: KJ0-4282

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