Revised on July. 3.

2024
Revised on July. 3. 2024

Measurement principle
Chemiluminescence resonance energy transfer (CRET) and neighbor-joining techniques are

widely used for bioanalytical sensors. Under alkaline hydrogen peroxide conditions, acridine

ester (AE) is oxidized to the substrate state, releasing an intense chemiluminescence signal

upon return to the ground state. Graphene oxide (GO) with excellent quenching ability can

CRET with AE-labeled DNA3, making the chemiluminescence almost non-existent and

achieving a "signal off" signal transition. The addition of the target detector induces an

adjacent contact reaction, forming an adjacent complex that detaches the AE-labeled DNA3

from GO and inhibits the CRET process. In addition, in situ cycling using nucleic acid

endonucleases can further amplify the signal and achieve highly sensitive

chemiluminescence detection.

The core detector device of this instrument is a photomultiplier tube (PMT), detected by a

single photon and transmitted to an amplifier, and add high voltage current amplification,

the amplifier will be converted from analog current to digital current, digital current will

transmit the luminescent signal to the main board and be calculated to obtain the relative

light unit (RLU), and then calculated by the standard curve to obtain the concentration of

the antigen or antibody to be measured in the sample.

Revised on July. 3. 2024

Overview of system unit

Front view

Rear view

Side view
Revised on July. 3. 2024

Indicator status

Normal Operating Error

Software basis
Menu structure
Revised on July. 3. 2024

Status bar

INSTALLATION
POWER SUPPLY

Applicable power supply: AC110~220V±22V，Frequency: 50±1Hz;

Power rating: 120W

OPERATIONAL ENVIRONMENT

C5000 should be placed on a dry, clean, flat and horizontal surface in an in-door setting

away from direct sunlight, vibration and any source of electromagnetic radiation.

Temperature：10 ~ 30 ℃,

Relative humidity: 30% ~ 70%.

INSTALLATION PRECEDURE

Connect the AC adapter and press the on/off

switch button
Revised on July. 3. 2024

System login

Username: User

Passcode: 1

Wait for the system to complete initialization

and self-checking routine

Prepare the external fluid rack and tubing

adapters
Revised on July. 3. 2024

Go to Apply>Consumable, press the SCAN

button to activate the built-in scanner, read

the QR via the built-in scanner to register

the consumable

Connect the external fluid rack to the system

via tubing adapters (UA: substrate, UB: buffer,

Waste: waster container)

Go to Apply>Liquid cleaning
Revised on July. 3. 2024

Press the DRAIN button to perform liquid

draining procedure

Test procedure
1 Calibration

1.1 Find the calibration QR card inside the

test kit

1.2 Open Reagent panel, press the SCAN

button and scan the QR to add the master

curve of current lot reagent.

Revised on July. 3. 2024

2 Assay protocol

(Wait for the incubation temperature rise to 37°C±5)

2.1 Prepare the reagent cuvette and diluent

tube (if necessary), make sure all the

reagent lyophilized beads stay at the

bottom

2.2 Use the pipette to add the sample to the

reagent cuvette

（Please refer to the assay specification for

detailed sample volumes）

2.3 Go to Test menu. Lift up the lid of

detection chamber, place in the reagent

cuvette and close the lid.

Revised on July. 3. 2024

2.4 Assay item can be identified automatically.

Press the CONFIRM button to place the test

order.

2.5 If you wish to continue place another

assay item to the test order, press the ADD

ITEM button and repeat 2.2 and 2.3. When all

finished, press the CONFIRM button to place

the test order.

2.6 When the assay is finished, select the

corresponding test position to remove the

reagent cuvette.
Revised on July. 3. 2024

Results and report

When the assay is completed, the analyzer

can automatically print the assay report via

the built-in printer. Assay report can be

also printed out manually on the built-in

printer or the external printer by pressing

the PRT button in the result menu.

Go to Apply>User setting.

Select Auto print checkbox to enable auto

print function.

Select Built-in printer checkbox to enable

the built-in printer.

Revised on July. 3. 2024

Maintenance
Please shut down the system daily to keep the analyzer in good condition.

Remove all the reagent cuvettes when the system is shut down.

Be sure to check on the remaining of the substrate and buffer before placing a test order.

Be sure to check on the waste container, empty the liquid inside if necessary.

Run liquid draining procedure whenever reconnecting the tubing adapters.

Sample collection and preparation

Serum collected using standard sampling tubes or tubes containing separating gel.

After collection of the whole blood, allow the blood to clot by leaving it undisturbed at

room temperature for at least 15-30 minutes.

Heparin treated plasma is preferred over EDTA.

Sample with visually inspected hemolysis, lipemia, or icterus should be considered for its

potential interference against assay outcomes.

Sample Stability: Sample retrieved from the refrigerator should be left undisturbed till it

reaches room temperature. Only 1 freeze / thaw circle is allowed.

Hemolytic indices following different degrees of hemolysis.*

*Ni J, Zhu W, Wang Y, Wei X, Li J, Peng L, Zhang K, Bai B. A Reference chart for clinical biochemical tests of hemolyzed serum samples. J Clin
Lab Anal. 2021 Jan;35(1):e23561.
Revised on July. 3. 2024

FQA
What shall be done when the system fails to identify the assay item when the reagent

cuvette is placed in?

Make sure the calibration is added. Remove the reagent cuvette, switch to the other side QR

and place it in again.

Do I need to scan the calibration every time when placing a new test order?

No. Scan the calibration only once when a new lot test kit is opened.

Why does the assay result show F?

This error occurs when little chemiluminescence signals are detected. Check on the

substrate remaining, replace a new bottle of substrate if necessary. Also, makes sure all the

reagent beads a dissolved when pipetting sample.

What shall be done when the reaction tray fails to respond?

Switch off the analyzer and rotate the reaction tray manually. Clean the remaining liquid

inside the test positions if necessary.

Revised on July. 3. 2024

Nanjing Poclight Biotechnology Co., Ltd

nd
South Side, 2 Floor, Building A02, Biopharmaceutical Valley Accelerator (Phase III), No. 142 Huakang Road, Jiangbei New District,
Nanjing City, Jiangsu Province, P.R. China.
Tel: +86-25-58255771
Website: www.poclightbio.com

HUMISS TRADING S. L.
Calle Luis Buñuel 12, MADRID 28018,Spain