Experiment

M o u n t P reparati on
Tem p o r a r y

OBJECTIVE
o u n t o f a le af p ee l to sh o w st o m at a.
ra ry m
To p re p ar e a te m p o

he
THEORY n ee d su n li g h t fo r p h o to sy n th es is . T
es
o f th e p la n t" . L ea v e o f th e p la n t
Leaf is th e "k it ch en ov er th e en ti re su rf ac
is th e o u te rm o st la y er th at ex te n d s a tr an sv er se section,
epid er m is ti ss u e ch y m at o u s. In
al ce ll s ar e th in w al le d an d p ar en m in u te o p en in g s called
bo dy . E pi de rm er m is co n ta in s
ar m o re o r le ss re ct an g u la r. E p id cu ti cu li se d called "cuticle
".
they appe is th ic k an d
a" . T h e o u te r w al l o f ep id er m is n er ti ss ue s ag ai n st excessive
"stomat e in
te ct iv e in n at u re . It p ro te ct s th ag ai n st ev en g ra zi n g
Epide rm is is p ro so m e ca se s
ac te ri al a n d fu n g al at ta ck an d in o u n d ed b y special cells
evap or at io n, b ep id er m is su rr
m at a ar e sm al l p o re s in th e lo w er co n ta in chloroplast. Their
an im als. S to Gu ar d ce ll s
ar d ce ll s" . T h ey ar e b ea n sh ap ed . tr o l o p en in g an d closing of
called "g u in . T h ey co n
ls ar e th ic k a n d o u te r w al ls ar e th w it h th e h el p of p ai r of
g u ar d
inner wal en an d cl os e
S to m at a h av e a m ec h an is m to o p
stomat a.
cells.

ED
MATERIAL R E Q U IR ,,. .__ _ _
Le af ap ex

f p la n t li k e li ly , p la in sl id e s, rgin
Leaf o sh , w - - - - Leaf ma
Petrid•18h , co v er sl ip , fo rc ep s, b ru I..;;J.~--
Le af bla de

needle.s ' a parr · f sc is so rs , fi lt er p ap er ,

Safr o t- -M id -r ib
"· ~' 11 !- '-b -= --
anine an d g ly ce ri n e.
Petiole

:~OCEDURE : ~---Lea
f ba se

e a fr es h li ly le af a n d p ee l o ff it
Tak
om it s u n d e r su rf ac e. P u t th e
fr
la ss co n ta in in g
Peel in a w at ch g
water.
 St om a
__ __ __ Gu ard

ce lls

Op en sto ma ta

Open an d clo se d sto m at

a (U nd er lo w po we r of
m ic ro sc op e.

~
~ tbsictiaty cen ~

Gu ard ce ll
Ou ter )
thm wa ll

Inn er
thi ck
•..:.-;~..--

~v
. \ ....

Op en an d clo se d st\ lm
at a lU nd er hi gh pv we r
of m icr os co pe ).
2.. A dd a few dr op s of sa fr
an in e st ai n in to th e w at
3. ch gl as s to st ai n th e pe el
Pu t th e pe el on a cl ea n .
sh ap e. sl id e af te r st ai ni ng an d
cu t it in a re ct an gu la r
or sq ua re
4. R em ov e th e excess of st
ai n an d w at er w it h th e he
5. lp of a fil te r pa pe r.
Pu t a dr op of gl yc er in e
us in g ne ed le to pr ev en on th e pe el an d co ve r it ca re fu lly w it h a co
t ai r bu bb le s. ve r sl ip by
6. Wait for ab ou t 2 m in ut es
for th e pe el to ta ke up th
7. e st ai n.
R em ov e th e excess gl yc
er in e w it h he lp of a filte
r pa pe r.
O BS ER V A TI O N S
1.
E~ i~ en ni s is m ad e of a
di st in ct cell walls. si ng le la ye r of ce lls th at
ar e cl os el y pa ck ed to ge th er w ith
 - ,-,~, •ure s are seen Whi
stom ata. ch are emb edd ed in the
~
.d
3. Eac h stom ata is gua rde d b ep1 erm al cells called
.
ya
4. Inn er wal l of gua rd cell s is thi pai
k
r of kid ney sh '
ape d gua rd cells' .
5. Eac h gua rd cell has a nuc leu s c era d
sco mp d
are to oute r wall.
an man y chlo ropl asts .
RESULT :
Lea f pee l has sto ma ta dist ribu ted in it.

PRE CA UT ION S:
1. The epi der ma l pee l sho uld be take n from a fres
h leaf.
2. Alw ays hol d the slid e by its edg es.
3. Alw ays use a bru sh to tran sfer the pee l to the
slid e.
4. Nev er fold the pee l.
5. Alw ays use a clea n slid e and a cov er slip .
6. Pla ce the cov er slip on the slid e gen tly and
grad uall y to avo id the entr y of air
bub ble s.

Viv a-V oce ~@ ~ @@ @

Q.l . Wh a t is s t o m a t a?
Ans. Sto mat a are min ute -po res pre sen t in leav es gua
rde d by gua rd cells.
Q .2. Wh a t are gua r d cell s?
.
Ans . The y are kid ney -sh ape d cell s surr oun din g the
stom ata! ope rung .
Q . 3. Wh i ch par t of l eaf h as s to m ata?
Ans . Epi der mis
. . 1
Q . 4. Why i s the pee l of l e af kep t in wat er b efor e sta1n1ng .
Ans . To pre ven t it from dry ing .
.
Q.s. N am e the par t of cell whi ch is colo ure db y s afra n1n .
Ans . Cel l wal l.
Q . 6. Wh at is the role of s t oma ta?
Ans . The y hel p in exc han ge of gas es.