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PRELIM-LABORATORY_2-PROPER-HANDLING

The document outlines a laboratory activity focused on the proper handling and usage of a compound light microscope. It includes objectives, materials needed, detailed procedures for familiarization, handling, focusing, adjusting light, preparing specimens, and cleaning up, along with safety precautions and questions for understanding. The activity aims to teach students the correct techniques for effectively observing and documenting specimens under a microscope.

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littleweeb01
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10 views

PRELIM-LABORATORY_2-PROPER-HANDLING

The document outlines a laboratory activity focused on the proper handling and usage of a compound light microscope. It includes objectives, materials needed, detailed procedures for familiarization, handling, focusing, adjusting light, preparing specimens, and cleaning up, along with safety precautions and questions for understanding. The activity aims to teach students the correct techniques for effectively observing and documenting specimens under a microscope.

Uploaded by

littleweeb01
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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NAME: DATE:

YEAR AND SECTION: Prelim Laboratory 2

Laboratory Activity: Proper Handling of the Microscope

Objective: To learn and practice the correct techniques for handling and using a compound

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light microscope.
Materials:
• Compound light microscope • Prepared slides (e.g., bacteria, protozoa,
• Blank slides tissue samples)
• Lens paper • Cover slips

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• Water • Immersion oil (optional)
• Piece of newspaper or • Dropper
• scissor

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Procedure:

Part 1: Familiarization and Cleaning


1. Identify Microscope Parts: Locate and label the following parts of the microscope: Use
your submitted activity for reference.

smudges.

Part 2: Handling and Focusing


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2. Cleaning the Lenses: Use lens paper to gently wipe the objective lenses and the
eyepiece. Avoid touching the lenses with your fingers as this can leave fingerprints and

1. Carrying the Microscope: Always hold the microscope by its arm and base for support.
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Avoid carrying it by the stage or body tube.
2. Placing the Microscope: Place the microscope on a flat, stable surface. Ensure the
area is clean and free from dust.
3. Turning on the Light Source: Adjust the light intensity to a comfortable level.
4. Preparing the Slide: Place the prepared slide on the stage and secure it with the stage
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clips. Center the specimen under the objective lens.


5. Focusing with the 4x Objective: Start with the lowest magnification objective lens
(4x). Use the coarse adjustment knob to slowly move the stage up until the specimen
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comes into focus.


6. Focusing with Higher Magnifications: Switch to the next higher magnification
objective lens (10x). Use the fine adjustment knob to sharpen the focus. Repeat for
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higher magnifications (40x, 100x), always using the fine adjustment knob.
7. Using the 100x Objective (Oil Immersion): If using the 100x objective lens, place a
drop of immersion oil directly on the slide over the specimen. Carefully rotate the 100x
objective lens into position. Use the fine adjustment knob to focus.
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Part 3: Adjusting Light and Contrast


1. Adjusting the Iris Diaphragm: Open or close the diaphragm to control the amount of
light passing through the specimen. This can help improve contrast and visibility.
2. Adjusting the Condenser: Move the condenser up or down to focus the light onto the
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specimen.

Part 4: Preparing Sample Specimen


1. Using your scissor cut the smallest letter “e” you can find in the newspaper.
2. Place it in a blank slide and put a dropped of water using a dropper.
3. Then place the cover slip and observe it in the microscope. Avoid bubble formation.
4. Draw what you observed.
Microbiology and Parasitology
Calamba Doctors College
College of Nursing
Angelo Carlo B. Gulanes
Part 5: Observing Sample Specimen
1. Observing the Specimen: Carefully examine the specimen, noting its shape, size,
color, and any other features.
2. Drawing the Specimen: Make a detailed drawing of what you see, including labels for
important structures.

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Part 5: Cleaning Up
1. Removing the Slide: Carefully remove the slide from the stage and dispose of it
properly.
2. Cleaning the Lenses: Use lens paper to wipe the objective lenses and the eyepiece.
3. Turning off the Light Source: Cover the microscope with a dust cover.

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Safety Precautions:
• Always handle the microscope with care.
• Never touch the objective lenses or the eyepiece with your fingers.

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• Use lens paper to clean the lenses.
• Be careful when using the coarse adjustment knob, especially at higher magnifications.
• Never force the microscope or its parts.
• If you have any questions or concerns, ask your instructor for assistance.

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Questions:
Part 1 Familiarization and Cleaning
1. What are the main parts of a compound light microscope?
2. Why is it important to clean the microscope lenses before and after use?
3. What type of material should be used to clean the microscope lenses?
Part 2: Handling and Focusing
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1. How should you carry a microscope to avoid damaging it?
2. What is the proper way to place a microscope on a work surface?
3. Why is it important to start with the lowest magnification objective lens when focusing?
4. What is the difference between focusing with the 4x objective and the 100x objective?
5. What is immersion oil used for, and how is it applied?
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Part 3: Adjusting Light and Contrast


1. How does the iris diaphragm affect the amount of light passing through the specimen?
2. What is the role of the condenser in focusing light onto the specimen?
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Part 4: Preparing Sample Specimen


1. What is the purpose of adding a drop of water to the slide before placing the "e"? What
problems might occur if you skipped this step?
2. Describe the proper technique for placing a coverslip onto a wet mount slide. Why is it
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important to avoid trapping air bubbles?


3. What magnification did you use to observe the "e," and why did you choose that
magnification? Would different magnifications reveal different details?
Part 5: Observing and Drawing
1. What are some important features to observe when examining a specimen under the
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microscope?
2. Why is it helpful to draw the specimen you are observing?
Part 5: Cleaning Up
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1. What are the steps involved in cleaning up after using a microscope?


2. Why is it important to cover the microscope with a dust cover when not in use?
Additional Questions:
1. What are some common mistakes people make when handling a microscope?
2. What are some safety precautions to keep in mind when using a microscope?
3. How can you improve your skills in using a microscope?

Microbiology and Parasitology


Calamba Doctors College
College of Nursing
Angelo Carlo B. Gulanes

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