PRELIM-LABORATORY_2-PROPER-HANDLING
PRELIM-LABORATORY_2-PROPER-HANDLING
Objective: To learn and practice the correct techniques for handling and using a compound
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light microscope.
Materials:
• Compound light microscope • Prepared slides (e.g., bacteria, protozoa,
• Blank slides tissue samples)
• Lens paper • Cover slips
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• Water • Immersion oil (optional)
• Piece of newspaper or • Dropper
• scissor
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Procedure:
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1. Carrying the Microscope: Always hold the microscope by its arm and base for support.
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Avoid carrying it by the stage or body tube.
2. Placing the Microscope: Place the microscope on a flat, stable surface. Ensure the
area is clean and free from dust.
3. Turning on the Light Source: Adjust the light intensity to a comfortable level.
4. Preparing the Slide: Place the prepared slide on the stage and secure it with the stage
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higher magnifications (40x, 100x), always using the fine adjustment knob.
7. Using the 100x Objective (Oil Immersion): If using the 100x objective lens, place a
drop of immersion oil directly on the slide over the specimen. Carefully rotate the 100x
objective lens into position. Use the fine adjustment knob to focus.
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specimen.
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Part 5: Cleaning Up
1. Removing the Slide: Carefully remove the slide from the stage and dispose of it
properly.
2. Cleaning the Lenses: Use lens paper to wipe the objective lenses and the eyepiece.
3. Turning off the Light Source: Cover the microscope with a dust cover.
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Safety Precautions:
• Always handle the microscope with care.
• Never touch the objective lenses or the eyepiece with your fingers.
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• Use lens paper to clean the lenses.
• Be careful when using the coarse adjustment knob, especially at higher magnifications.
• Never force the microscope or its parts.
• If you have any questions or concerns, ask your instructor for assistance.
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Questions:
Part 1 Familiarization and Cleaning
1. What are the main parts of a compound light microscope?
2. Why is it important to clean the microscope lenses before and after use?
3. What type of material should be used to clean the microscope lenses?
Part 2: Handling and Focusing
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1. How should you carry a microscope to avoid damaging it?
2. What is the proper way to place a microscope on a work surface?
3. Why is it important to start with the lowest magnification objective lens when focusing?
4. What is the difference between focusing with the 4x objective and the 100x objective?
5. What is immersion oil used for, and how is it applied?
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microscope?
2. Why is it helpful to draw the specimen you are observing?
Part 5: Cleaning Up
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