JWT 115 CHEMISTRY PRACTICAL GUIDE Booklet (1)
JWT 115 CHEMISTRY PRACTICAL GUIDE Booklet (1)
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Table of Contents
1.0 Introduction ....................................................................................................................1
1.1 Attendance ........................................................................................................................... 1
1.2 Lab Preparatory Tasks ....................................................................................................... 1
1.3 The Practical Activity ......................................................................................................... 2
1.4 Practical Report .................................................................................................................. 2
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1.0 Introduction
Practical work is divided into three components: i) Pre-laboratory task (10 marks); ii)
During the practical and iii) post-laboratory task. All three tasks are compulsory, the first
task always has 10 marks and it is written before doing the practical work in the laboratory.
The second part is the practical work itself and you will be scored by the teaching assistant
during the tasks and experiments in the laboratory. The last part is the writing of the
practical report which will be handed to the teaching assistant a week after doing the
practical.
1.1 Attendance
Practical work sessions are scheduled every fortnight from the beginning of the semester.
All sessions are compulsory and no opportunity will be given for any absenteeism unless
evidence of an authentic doctor’s note is produced.
All students are required to complete the pre-laboratory task (Appendix A) for each
practical before being allowed in the laboratory. The tasks should be handed to the
teaching assistant in your laboratory venue. No pre-laboratory task NO ENTRANCE into
the laboratory venue.
The Pre-laboratory work should be arranged according to the template that you find on
ClickUp under Laboratory resources. The structure of the template is as follows:
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1.3 The Practical Activity
There are six practical activities contained in this guide. You are required to familiarise
yourself with these experiments through research before coming to the laboratory and by
completing a pre-laboratory task. You will do the experiments in groups and you are
expected to be an active participant in your group as you will be scored by your TA using a
rubric that is at the end of your pre-laboratory task. Please, read and understand the
laboratory safety rules (Appendix B) whose soft copy you can find on ClickUp under
Laboratory resources.
A laboratory report is a written document that explains and analyses a practical work that
involves a scientific concept. It outlines a practical work's goal, techniques, results,
discussions, and conclusions. The main objective of the report is to demonstrate your
understanding of the processes of the scientific method by executing and assessing a
hands-on practical activity. Since parts of the report have been covered in your pre-
laboratory task there is no reason to include them in the practical laboratory report. Follow
the practical report template as shown in Appendix C. You end your report by answering
the questions that are asked in each practical activity.
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2.0 Practical 1- Flame tests
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Aim: To determine what colour a metal cation will cause a flame to be.
Apparatus:
Be careful when working with Bunsen burners as you can easily burn yourself. Make sure
all scarves/loose clothing are securely tucked in and long hair is tied back. Ensurethat you
work in a well-ventilated space and that there is nothing flammable near the open flame.
Results: Record your results in a table, listing the metal salt and the colour of the
flame.
Metal Salts Colour Metal Powder Colour
NaCl Copper
CuCl2 Magnesium
CaCl2 Zinc
KCl Iron
Discussion
The above experiment on flame tests relates to the line emission spectra of the metals.
Question
What is the significance of the line emission spectra in the experiment on a flame test
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3.0 Practical 2 – Heating curve of water
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Method:
Warning: Be careful when handling the beaker of hot water. Do not touch thebeaker
with your hands, you will burn yourself.
Results:
Heating of ice
Time (s) Temperature (oC) Time (s) Temperature (oC)
1 16
2 17
3 18
4 19
5 20
6 21
7 22
8 23
9 24
10 25
11 26
12 27
13 28
14 29
15 30
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2. Plot the graph of the heating curve water.
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3.2 Explain the observation above. (2)
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4.1 Name the process in which matter change from solid to liquid. (1)
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4.2 Name the process in which matter change from liquid to solid. (1)
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4.0 Practical 3 – Conservation of matter
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Warning: Always be careful when handling chemicals (particularly strong acids like
hydrochloricacid) as you can burn yourself badly.
Materials:
Reaction 1:
Reaction 2:
Reaction 3:
Any effervescent tablet (e.g. Cal-C-Vita tablet), balloon; rubber band; mass meter;Test
tube; beaker
Method:
Reaction 1
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Reaction 2:
1. Solution 1: Dissolve 0.4 g of sodium hydroxide in 100 ml of water. Add a few dropsof
bromothymol blue indicator to the solution.
5. Determine the mass of hydrochloric acid added. (You do this by weighing the
remaining solution and subtracting this from the starting mass)
6. Compare the mass before the reaction to the total mass after the reaction. What doyou
notice?
Reaction 3
5. Fit the balloon tightly to the test tube, being careful to not drop the contents into the
water. You can stand the test tube in a beaker to help you do this.
7. Lift the balloon so that the tablet goes into the water.
9. What do you observe about the masses before and after the reaction?
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Results: Fill in the following table for the total mass of reactants (starting materials)and
Reactants
Products
Add the masses for the reactants for each reaction. Do the same for the products.For each
reaction compare the mass of the reactants to the mass of the products.What do you notice?
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5.0 Practical 4 – Acids and Bases
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Titration is the name given to the procedure when an acid is reacted with a base to determine
the concentration of either the base or the acid.
Experiment: The skill you will encounter in this experiment is how to manipulate a burette
during titration. You will have to work the tap with your left hand while swirling the contents
of a conical flask with your right hand
Acetic acid (CH3CO2H(aq)) is the acid present in vinegar. In this part you will titrate acetic
acid against a standard solution of sodium hydroxide to find the concentration of acetic acid
in vinegar. Work in pairs. Do a rough plus at least three other titrations, swopping roles so
that you each get a chance to perform every operation. Combine your results and use the
average of the two readings for the final calculation.
Transfer the vinegar quantitatively to a 250 mL volumetric flask. (This means that you must
transfer every drop of the vinegar to a 250 mL volumetric flask.) To do this, add a little
distilled water to the vinegar in the measuring cylinder and swirl it around to mix. Put a glass
funnel into the neck of the volumetric flask and pour the solution in the measuring cylinder
through the funnel into the volumetric flask. Use your wash bottle to rinse the measuring
cylinder thoroughly so that the washings go into the funnel. Finally rinse the inside of the
funnel to make sure that any vinegar sticking to the inside of the funnel also goes into the
volumetric flask. Now carefully add distilled water up to the mark, put the stopper on and
gently turn the flask upside down a few times to make sure the contents are well mixed.
In a clean, dry beaker collect about 200 mL of the standardised approximately 0.2 mol L-1
solution of NaOH which has already been prepared. Use some of this solution to rinse out
your burette including the tap. (Pour this rinsing down the sink.) Now fill your burette with
the standard NaOH solution, make sure the tap is full and make a note of the burette reading.
You are now ready to perform the titration.
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How to do the titration:
Transfer your vinegar solution from the volumetric flask to a clean 250 ml beaker. Use a
pipette to transfer 20.00 mL of the vinegar solution from the volumetric flask to a clean 250
mL conical flask. Add two drops of Phenolphthalein indicator. You must now titrate the
vinegar solution in the conical flask with the sodium hydroxide in the burette, swirling the
flask all the time with your right hand.
You will be able to add NaOH fairly fast at first until you notice that the colourless colour
changes light pink and does not disappear immediately. Now continue adding the base drop
by drop until the light pink colour just remains. This is the end point. If the solution turns
pink, you have added too much NaOH and there is nothing you can do to fix it! Take the
burette reading at the end point and record it.
Repeat the titration at least three times and clean up before doing any calculations.
IMPORTANT: rinse the burette out very well with tap water to remove all traces of NaOH(aq)
or else the next person using it will encounter problems with the tap sticking.
Now present the results of your titrations in a table similar to the one below.
Titre 1 2 3
(6)
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Questions to be answered individually.
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6.0 Practical 5 – Galvanic cells Zinc-Copper Cell
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Aim:
MATERIALS:
INSTRUCTIONS:
Pour about 200 ml of the zinc sulphate solution into a beaker and put the zinc electrode
into the solution.
1. Pour about 200 ml of the copper sulphate solution into the second beaker and placethe
copper electrode into the solution.
2. Fill the U-tube with the sodium sulphate solution and seal the ends of the tubes withthe
cotton wool. This will stop the solution from flowing out when the U-tube is turnedupside down.
3. Connect the zinc and copper electrodes to the ammeter. Does the ammeter recorda
reading?
4. Place the U-tube so that one end is in the copper sulphate solution and the other end is
in the zinc sulphate solution, as shown in the diagram.
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Is there a reading on the ammeter?
5. Remove the ammeter and insert the LED bulb in the circuit. Does it glow?If not, try
connecting a few cells in series until the LED lights up.
6. Observe what is happening at the copper electrode and at the zinc electrode.
In the last activity, we demonstrated a zinc-copper cell. This is made up of a zinc half-cell
and a copper half-cell. Together, they make up the whole cell. The purpose of the U-tube
is to connect the two half cells. It is called the salt bridge.
TAKE NOTE: You can also use a voltmeter to measure the potential difference
across the cell. The voltmeter will replace the ammeter and LED light.
How do we explain the chemical reactions taking place in the zinc-copper cell?
When a zinc sulphate solution containing a zinc plate is connected by a U-tube to a copper
sulphate solution containing a copper plate, reactions occur in both solutions.
• At the zinc electrode, the zinc metal has gone into the zinc sulphate solution as zincions.
• At the copper electrode, copper ions from the solution have deposited onto the
electrode as copper metal atoms.
In the zinc-copper cell the important thing to notice is that the chemical reactions thattake
place at the two electrodes cause an electric current to flow through the outer circuit. In this
type of cell, chemical energy is converted to electrical energy.
As we have said before, an electric battery used in appliances such as a torch consistsof two
or more electric cells connected. There are many different battery celltypes such as zinc-
carbon, nickel-cadmium, and nickel-zinc batteries.
QUESTIONS:
1. What did you notice on the ammeter (or voltmeter) when you connected the circuitwith
the U-tube?
2. What does the ammeter reading tell us?
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7.0 Practical 6 - Organic chemistry- melting and boiling points
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Aim:
Apparatus:
Method:
WARNING!
Ethyl methanoate can irritate your eyes, skin, nose and lungs. Keep open flames
away from your experiment and make sure you work in a well ventilated area.
1. Label the test tubes 1, 2 and 3. Place 20 ml of butan-1-ol into test tube 1, 20 ml of
propanoic acid into test tube 2, and 20 ml of ethyl methanoate into test tube 3.
2. Half-fill the beaker with cooking oil and place it on the hot plate.
3. Place the thermometer and the three test tubesin the beaker.
4. Make a note of the temperature when eachsubstance starts to boil.
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Results:
Fill in the gaps in the table below. Do the values you obtained match those reported in
literature?
Molecular formula
Molecular mass
Type of
intermolecular
forces
Literature boiling
point (oC)
Draw the structural representation of ethyl methanoate, butan-1-ol and propanoic acid.
You should have found that the ethyl methanoate boiled first, then the butan-1- ol and then
the propanoic acid.
Ethyl methanoate has some dipole-dipole interactions but cannot form a hydrogen bond.
The alcohol (butan-1-ol) can form hydrogen bonds and so has a higher boiling point. This
strong intermolecularforce needs more energy to break and so the boiling point is higher.
For propanoic acid hydrogen bonds form between the carbonyl group on one acid andthe
hydroxyl group on another. This means that each molecule of propanoic acid canbe part of
two hydrogen bonds (this is called dimerisation, see Figure 4.57) and so theboiling point is
even higher for propanoic acid than for butan-1-ol.
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Appendix A: Pre-Laboratory Task
Lecturer: __________________________________
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Theory of the Practical Work
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List of apparatus
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Safety Precautions
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Flow diagrams
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Pre-Laboratory rubric
Criteria 0 1 2 3 Comments by
Tutor
Flow Student completed some Logical sequence was Evidence that the
diagrams of the steps of the lab, but followed, but missing procedures were
not in order. steps. The flow diagram followed completely
Flow diagram does not used is incomplete and and in logical
show a logic sequence. distracting. sequence.
The flow diagram is
complete with no steps
missing.
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Appendix B: laboratory safety rules
It is critical that you prepare for each experiment by reading it carefully before entering
the laboratory. Not only will this ensure that you get the maximum benefit of the
experience, but it also makes for a safer environment in the laboratory. This is important
not only for your own safety but also for those around you. A number of policies have
been developed in order to make sure that the laboratory is safe and that it runs
smoothly. In each experiment specific hazards are indicated by bold type and
procedures are described that must be adhered to. Accidents commonly occur when the
following rules are not followed.
The following rules are designed for your safety in the laboratory. The Laboratory
Instructor (LI) is required to enforce these rules and has the full backing of the Division
and Faculty. The LI is also required to enforce all laboratory experiment-specific safety
procedures in carrying out the laboratory work. Violations of these rules will result in
expulsion from the laboratory.
1. No one is allowed in the laboratory without the supervision of a LI. No laboratory work
will be done without supervision. Perform only authorized experiments, and only in the
manner instructed. DO NOT alter experimental procedures, except as instructed.
2. Approved safety glasses must be worn by all persons at all times and they may not be
modified in any manner. NO SAFETY GLASSES NO ENTRY INTO THE
LABORATORY!
4. Lab Coats of 100% cotton are REQUIRED upon entering lab. NO LAB COAT NO
ENTRY INTO THE LABORATORY!
5. Absolutely NO food or drinks are allowed in the laboratory. This prohibition applies to
the storage of food and the consumption of food, beverages, medicines, tobacco,
and chewing gum. Contact lenses and cosmetics are not to be applied while in the
laboratory. Infractions will result in expulsion from the laboratory. Because cell phones
or other personal electronic media can easily be damaged in the laboratory, use of such
devices is not allowed in the laboratory.
6. Learn the location and how to operate the nearest eyewash fountain, fire
extinguisher, and fire alarm box. First aid for acid or base in the eyes is to wash with
copious amounts of water using the tubing attached to the tap for 15 minutes; then
go immediately for further treatment. First aid for acid or base on skin or clothing is to
wash thoroughly with water for 15 minutes. Use the above tubing on tap as a shower if
appropriate, removing contaminated clothing for thorough washing.
7. All operations in which noxious or poisonous gases or vapours are used or produced
must be carried out in the fume hood.
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8. Confine long hair while in the laboratory. Hair can catch on fire while using open
flames.
9. Mouth suction must never be used to fill pippettes. Always use pipette fillers.
10. All accidents, injuries, explosions, or fires must be reported at once to the TA In case
of serious injury, the TA or Lab Supervisor must call 911 for an ambulance.
11. Horseplay and carelessness are not permitted and are cause for expulsion
from the laboratory. You are responsible for everyone's safety.
12. Keep your working area clean – immediately clean up ALL spills or broken glassware.
Exercise appropriate care to protect yourself from skin contact with all substances in the
laboratory. Clean off your lab workbench before leaving the laboratory.
Skateboards, rollerblades, and other such personal equipment must be stored outside of
the laboratory. Personal electronics are only permitted when needed for the laboratory.
13. Put all toxic or flammable waste into the appropriate waste container(s) provided in
your laboratory.
14. Containers of chemicals may not be taken out of the laboratory . Never take
uncapped glassware containing chemicals into the hallways or other public areas.
15. Laboratory doors must remain closed except when individuals are actively entering or
exiting the lab. DO NOT prop the door open with chairs, stools, or any other objects.
16. Gloves will be provided, when toxic chemicals are being used. Gloves are presumed
to be contaminated and must not come into contact with anything outside the laboratory
except chemical containers.
17. Specific permission from your the Lecturer is required before you may work in
any laboratory other than the one to which you have been assigned. Only laboratory
rooms where the same laboratory course is operating may be used for this purpose.
18. If you have a special health condition (asthma, pregnancy, etc.) or any personal
health concerns, consult your doctor before taking chemistry lab.
19. If you come to the laboratory with non-compliant goggles, shoes, or clothing, you will
not be allowed to work in the laboratory. In that context, note that THERE ARE NO
MAKE-UP LABORATORIES. Your course grade will be significantly lowered or you may
fail the course if you do not meet the dress code.
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Appendix C: Practical Report Template
Introduction
The purpose of this experiment was…
- Derive your introduction from the aims and objectives which you get from your
research about the practical work.
- Outline the purpose of the practical work.
- Practical hypotheses and Variables.
- The introduction should be just a paragraph between 100 and 150 words only. be
a few sentences long.
- remember everything in this report is past tense.
- no “I’’ or “We” in a practical laboratory report, replace them with “the student” and
“the group”.
Experimental Procedures
This should be returned in past participle tense and do not copy-paste every step
from the lab manual, paraphrase step by step the way you have carried the practical
in your own words. The procedure should be accompanied by diagram representation
of the final experiment set up.
Explain your results in terms of the theoretical background you provided in your pre-
laboratory report.
Analyse and infer what is the meaning of the data presented in the tables and
graphs.
3. Conclusion
After you present your conclusion, provide a short justification of the results. Discuss
the implications of your conclusion.
4. References
Pavelich, M. J., & Abraham, M. R. (1979). An inquiry format laboratory program for
general chemistry. Journal of Chemical Education, 56(2), 100.
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Rudd II, J. A., Greenbowe, T. J., & Hand, B. (2001). Recrafting the general chemistry
laboratory report. Journal of College Science Teaching, 31(4), 230.
Bhandari, P. (2022). How to write a lab report: step-by-step guide and examples.
Retrieved 16 February 2023 from the World wide web https://www.scribbr.com/academic-
writing/lab-report.
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