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Agar Plate Lab Manual

This manual provides a step-by-step guide on how to create agar plates using homemade materials for culturing microorganisms. It explains the purpose of agar in microbiology, its use for growing bacteria, and outlines the necessary materials and procedures for making and sterilizing agar medium. The document includes detailed instructions for pouring agar plates and storing them properly.

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12 views

Agar Plate Lab Manual

This manual provides a step-by-step guide on how to create agar plates using homemade materials for culturing microorganisms. It explains the purpose of agar in microbiology, its use for growing bacteria, and outlines the necessary materials and procedures for making and sterilizing agar medium. The document includes detailed instructions for pouring agar plates and storing them properly.

Uploaded by

theuriitime
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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ATOM ALLIANCE

8 - Eddington

Agar Plates Manual

Group Members:
Alvarez, Cassiel Jamie
Frias, Lorraine Kristel
Macalalad, Marco Santino
Trangia, Julian Mari
Villadolid, Ayessa Amanda
Yao, Allaine Reese
Table of contents
 Introduction
 What is an Agar plate?
 What is the purpose of Agar in Microbiology?
 What is Agar used for bacteria?
 Materials
 Procedure

Introduction
In this manual, we will show you how to make an Agar plate using
homemade materials.

What is an Agar plate?


An agar plate is a Petri dish that contains a growth medium solidified with
agar, used to culture microorganisms, Sometimes selective compounds are added
to influence growth, such as antibiotics.

What is the purpose of Agar in


Microbiology?
Due to its nature, agar is an aseptic and solidifying component, which
makes it an ideal candidate to use in microbiology. Technicians must use agar to
create their culture media. Agar is mixed with nutrients to create the perfect
conditions for the development of a microorganism.
What is Agar used for bacteria?
Agar is commonly used in the laboratory to help feed and grow bacteria
and other micrgoorganisms. It acts as a culture that provides nutrients and a place
for these items to grow, but since it is indigestible to the microorganisms, they
cannot eat and destroy it.

Materials
 1 beef bouillon cube

 2 teaspoons (8 g) of sugar

 1 cup (240 ml) of distilled water

 1 packet (2 1/2 teaspoons or 7 g) of unflavored gelatin

Procedure
I. Making and Sterilizing an Agar
Medium
1. Heat a beaker and Petri dishes in a 350 °F (177 °C) oven for 2 to 3
hours.

2. Pour the medium ingredients into the beaker.

3. Bring the mixture to a boil on a hot plate for 1 minute.

4. Cool the agar medium to 120 °F (49 °C).


II. Pouring Agar Plates
1. Sanitize your workspace with isopropyl alcohol or diluted bleach.

2. Label the bottom of each Petri dish with experiment information.

3. Flip the Petri dishes and wipe the beaker's mouth with an alcohol

wipe.

4. Pour 1⁄4 in (0.64 cm) of medium into each dish and set the lids on.

5. Leave the dishes for about 30 minutes so the agar sets.

6. Turn the dishes upside-down and store them in the fridge.

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