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Complete CW and HW and Submit Tomorrow. Learn the Next 10 Conversions Along With Intext and Book Exercise for a Test. Procedure Can Be Written During the Week End.1740592012279

The document outlines a procedure for separating colored components in a mixture of red and blue inks using ascending paper chromatography. It details the materials needed, the principle of differential adsorption, and the step-by-step procedure to calculate the Rf values for each dye. The results show Rf values of 0.2 for red dye, 0.6 for purple dye, and 0.8 for blue dye.

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7 views

Complete CW and HW and Submit Tomorrow. Learn the Next 10 Conversions Along With Intext and Book Exercise for a Test. Procedure Can Be Written During the Week End.1740592012279

The document outlines a procedure for separating colored components in a mixture of red and blue inks using ascending paper chromatography. It details the materials needed, the principle of differential adsorption, and the step-by-step procedure to calculate the Rf values for each dye. The results show Rf values of 0.2 for red dye, 0.6 for purple dye, and 0.8 for blue dye.

Uploaded by

dharaneshvnr
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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1.

Paper Chromatography

Aim: To separate the coloured components present in the mixture of red and blue inks by ascending
paper chromatography and to find their Rf values.

Materials and Chemicals Required: Whatman filter paper strip, beaker, scale, pencil, glass rod, paper
clip, distilled water, mixture of red and blue inks, fine capillary tube
Principle: It is based on the differential adsorption of the components on the adsorbent. The different
coloured dyes are adsorbed on the filter paper at different rates, based on their solubility with the given
solvent.
# Stationary Phase – Filter paper
# Mobile Phase – Solvent

Procedure:
• Take a Whatman filter paper strip and using a sharp pencil draw a horizontal line 4cm from one
end of the paper. Then draw another line vertically from the centre of the paper. Name the point
at which the two lines intersect as P.
• Place a drop of the mixture at the point P. Let it dry in air.

• Pour distilled water into a beaker, which is used as the solvent.


• Suspend the filter paper vertically in the beaker containing the solvent in such a way that the
pencil line remains about 2cm above the solvent level.
• Keep the jar undisturbed.
• Notice the rising solvent along with the different coloured dyes. After the solvent has risen about
15 cm, different spots are seen on the filter paper.
• Take the filter paper out and using a pencil, mark the distance that the solvent has risen on the
paper. This is called the solvent front.
• Dry the filter paper and mark at the centre of each coloured spots using a pencil.
• Measure the distance travelled by the solvent and that of the different spots from the original
line.
• Calculate the Rf (Retention Factor) values of the dyes using the formula,

LHS Chromatogram
LHS
Observation and Rf values
Distance travelled by the Distance travelled by
Rf value
component from the the solvent from the
S.No Components
original line (cm) original line (cm)
= A/B
(A) (B)
1 Red dye 2 10 2/10 = 0.2
2 Purple dye 6 10 6/10 = 0.6
3 Blue dye 8 10 8/10 = 0.8

RHS
RESULT
• Rf value of red dye = 0.2
• Rf value of purple dye = 0.6
• Rf value of blue dye = 0.8

Precautions:
1. Use a sharp pencil to draw the reference line.
2. Use a fine capillary tube.
3. Keep the jar undisturbed and covered during the experiment.
4. The spot should be small and rich in mixture.
5. Allow the spot to dry and keep the strip of filter paper straight with the tip touching the
solvent.

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