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Banana Dna Extraction

The document outlines a procedure for extracting DNA from a banana using common household items. It includes a list of materials, step-by-step instructions for preparation, lysis, filtration, and DNA precipitation, as well as guide questions for discussion. The objective is to observe the extracted DNA and understand the scientific principles behind the process.

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6 views2 pages

Banana Dna Extraction

The document outlines a procedure for extracting DNA from a banana using common household items. It includes a list of materials, step-by-step instructions for preparation, lysis, filtration, and DNA precipitation, as well as guide questions for discussion. The objective is to observe the extracted DNA and understand the scientific principles behind the process.

Uploaded by

dae
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Group Number: ________ Members: ____________________________________________________ Score:_________________

Banana DNA Extraction

Objective: To extract and observe DNA from a banana using everyday household items.

Procedure:
Materials:
 Preparation:
 1 ripe banana
o Peel and slice the banana, then place the
 Ziplock bags
slices in a ziplock bag.
 Dish soap
o Seal the bag and mash the banana until it
 Salt
becomes a smooth pulp.
 Rubbing alcohol (chilled in the freezer)
 Lysis Solution:
 Water
o In a small bowl, mix 2 teaspoons of dish
 Coffee filters or cheesecloth
soap, 1 teaspoon of salt, and half a cup of
 Clear glass or a beaker
water. This solution helps break down the
 Toothpicks or tweezers
cell membranes to release the DNA.

 Extraction:
o Add the lysis solution to the ziplock bag
GUIDE QUESTIONS:
with the mashed banana. 1.Explain how the lysis solution breaks
o Seal the bag and gently mix the contents open the cell membranes to release the
for about 5 minutes. DNA.
 Filtration:
o Place a coffee filter or cheesecloth over a
clear glass or beaker.
o Pour the banana mixture through the filter
to remove solid pieces, collecting the
liquid in the glass. 2.Discuss the role of salt in the process.
 DNA Precipitation:
o Slowly pour an equal amount of chilled
rubbing alcohol into the glass with the
banana liquid. Pour it down the side of the
glass to form a layer on top.
o Wait a few minutes for the DNA to 3.Talk about why rubbing alcohol is used.
precipitate out of the solution. You should
see white, stringy substances forming.
 Observation:
o Use a toothpick or tweezers to carefully
lift the DNA strands out of the solution.
o Observe the extracted DNA and note its
appearance.
Discussion:

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