0% found this document useful (0 votes)
6 views

pump, column, detectors in HPLC

The document discusses various types of pumps and columns used in analytical chemistry, including their mechanisms, advantages, and disadvantages. It details specific pump types such as screw-driven and reciprocating pumps, as well as different column packings and detector technologies used in chromatography. Additionally, it highlights the importance of sample handling and the characteristics of various detectors in the analysis process.

Uploaded by

Disha
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
0% found this document useful (0 votes)
6 views

pump, column, detectors in HPLC

The document discusses various types of pumps and columns used in analytical chemistry, including their mechanisms, advantages, and disadvantages. It details specific pump types such as screw-driven and reciprocating pumps, as well as different column packings and detector technologies used in chromatography. Additionally, it highlights the importance of sample handling and the characteristics of various detectors in the analysis process.

Uploaded by

Disha
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
You are on page 1/ 8

fumpingSplem

a constent too
fumps at
ot molile phaseto hecoluon
Requiumuas to
bollo w
bquwd cheomatagta phie pampi
soo0 psi (IL)
low tates |anging hom o to lo min
QFlouuuptoducibil ih'es o-5 oA bete
Resis tan ce to catosorn bya Natrety ok solveuts

Follaoinqtpes o pumps at Uscd in ApLe Systms


0Sceew- deivesn pump
These conssts ol- laege syeinge lke chombcs
equieped wrth a plunge actvaed by a sctew-deien
mechanism powued54 a motot
Adv Pulse-tee delieuy
Elow tate s ndepndt ot viscoity A
back ptassU
Disad- Limited salventcopauhy
not suls!e toe qeadant tion tecbaique
DATE

e Reciptocahing plmps
Usually consi'sts ot a small choamber n
which the solveutispumpd by the baek
o o motot- deven pistun.

To column
Motot Seal

Ball che -valve


Reutoahig
Pston 4 solvent
Pa Reca'ptucotg Pumg
Two wbich opm amd
close alhemately conthol te
solvat mto and out ok aylindes Th
solxtat dreet contactwththe
piston
Reiptoahgs
becouse o ollous
avata
vofmeC35-Lyo.
@high output ptusuCupto lo, ooo
to gtodit elahon
constaut Howtate
back pt1ss42e solsuat viatos ity
and
Sustees tim a
apulscd tlew cohick_ must be damped dosaduataye apo
pulss appean as. basline
thecheomato qzam..
9Întumatic pumps
conslats ok collapsihle Solvunt conta'nu
houned 'n a vesselthat can Le pesSzed
by compuised gas
Advantag
Disad vantasg
and ptessum output
odaptabil 6qeaditelaton s mot possit!
Samplenjecran
`ample tn
systeen
Sampe
-eSonple
Joo
Sample
Out

Eluent

-cheomatoqgeophlale
@A sanple Loop Column Igck sampk
od
Laz laApicada injehion systo
a stainlesssteel ting
with six consistslak
tocolum . A drtteeentepotts
movableteHon Cone
,
the ing has
ogniasemeuh
eacko whicbaconnsiapaieaot stecaal
pot Two ohthepottsau
steemaL.Smple loopo connehd by
trxedvolume
PolIntoneconbiquaton
dietHow heonepetmb.
otantueantntocelumt
cansbe
tlledwitl thel sempleTThe
Conecan
the
thest betotated 3oto make
3amplehkaopLapatt.a
okichswupshe sámplest Calumn maun stam
loops Thtsessyslemshaveioechamgeable
ttom ptondingto loo
a choice o
sample s'zes.
PAGE NC
DATE

Columhs toe PLC

Laud cheo madogkayphre columns.


constuchd teom smoo+h bot
nually
stau'nus steed
ubingThese Sometimnts made up o
Leav walled glau ubing and polym
(palyethewtheketonePEEK). SS colums
PEEK ane aso ola

Analtico columms
le-columns 5-2S m longIn
dioroe)aok anayteo columns is 3-5 mm.
3'es3-5U m Suckcolums
andpattide
4o,000 to o,000 plats met
lo,000platescolumn0*poibmo
Nowadoy colums. Mm panhida
diameke 3 1 Cnheigt 3-5
These haue 4he
25,000 platsealum
minmal solvent
geod.speed and
Consumian

Guacd cahimn
Itiswually shott and intoduad betot
analy ha celumn to
analyhial column by emonigpartnlat
mall cantamiaus tom the solv
also sample ompanuts hot bind
and
Statianayphae
Composhon quad column packiint
Aimila
wually latg
Gteoocolemn Anee sacuticed to ptotct

+natn Contol -
Iypes ot columnpackings
Two baslc types ot au wsd inLc.
packings
9)fotous
fellrcdo pahides wre spheu calj nonpot,
glas ot polymn beads urttdidmeha'o 30d Nm
A thinl patousloye ak scaalumira
a polstyne. drxinyl bumene ynthehe2ain
depas1 td on5uiteic at these beads
ThtseLppelliculan packings
kaplaud y oSmapotousmiceo patides
fellicla paueingsua tor
Seponahon ok peoBuins langea biomalecus
fotouni miceopatthds howe diamiki tang ng
tom3lo un These hawaNny
pathide sizdstebutionaee
Thtselpathicles at composedalasilace
olumna t:ot synthete tsinpolysyent
divioylbsmzine alas
sd Silica s by bae most ComnOn
tot lebiaune ot is hish suntaee aa
good d mechanical stngthSilrca patie
peapaudby alamtnot smann
silca pauthicsencconditons whichatgix
lagepatheles ok unboem diamet
Rasal tingpacheles coated with atqanc
tlms ohick auchemcall physitlly
bondedtote Subecins
hom tisulsduentwkich
Intative
ndea changes measUtes It
dekcto abo
teeunhalhattomeho
-cond deeetor calleddas
chiqueis
ae toe
nbbegened inepensiv
I latredy
enshvopand.
ntadration
beam theougkAmal
ispaised calumn he teom EHbluet
papulan deteors
mostNouaslezuvelyth
kansgtivdekcto 9Naiableeou
Lbitsng wewdeny selected
pes ty
ons inh'sa absaek moleales
a
nmounestom 254and2io
dengto Photomehtawrtha
todiogs thistcle
laeqetSensiiveto tacdiahian
is
Do
bancd at wich
idelusede
tectets most ate Thtse
tot ulta-xialet
ac O
nanoga micegam
to ihvit Sens
high
HPLCashoudbe
ol tot Detectots
Detetos
DATE
PAGE
It s vety senstive to chang1s in
atuict temptat conttor a taqwd
It
ehhon
ccnnot
due
be used etctvely orth
oa
jodhent
honge in basel ne The
change tekactve index of
soent changes
hehaseline.
It s a tuggeddekctoe and detct concentahbns
ot about lo sto lo:

2.
Phetodede attay detkcto
PDA deketots detoed entèL speceum
3imultaneausyA drode attay consss ok a
seties ot hundtds ot silicon photo diodes
posihoned snde by side `in gle sih con
Coystal oe chip. Each has an asoiatd
Alasnge copaeito that collects and integeates
the phatcuet ut qenatc cwhen photons stui'ke
the pho todiede tadiahon alispesed inte
its drteent waulengths tals on the suta
aea the diudeattaya spectum Can be
tecotded:APPAdeketo consis lo2.4
diode element jn
entimehas spacaok Couyteot
The instantaneaus decoedinq a
absouphon sprcha _peovides pownkal qualitahv
tool The tadinhonpasses hedugh the detctoz
How el ans dispesed hygzahng to
phatodiade toz detechor' Each
aztay a sepanate photodiode
wawebngts_talaon
amd spechum
No hie akleadantaqe ok ppA etecto ia
pea puuity and thus extmelywscbad toL
lemtihtatonokunkngwn swbstanus
oYalopping
PAGE NC

DATE

9 FuotsencA detctoes
Amptaveoselechihy i.
These detctoes give detectoesbecouát tew
cOmpounds bHou tham absaeb
Higly insthse delete toe tuoeLutara
cen poundsamd alnoiunetdnbianalytieal
Audics Rsbot lain thiamine Fludtui canit
Ampromehtmaybe
deivatives deeetospupand
echoNagts
by
<J-danayl cWod
usetul in dekehng alecttoetive.
substancus and tindsapphcabilty in balagizal
studres emHPLC Sepasathern and delechon
catcholam'n tom
otoh
oF teaaclequao thes
the beatn
go Thase dttots at batedalon ampuomeh,
yottammahyoulametuy andonbcomet
Advantuqes hgh sensikh wih usin pihci
wrdespeabdapplhcabiliky
theat 6ontunctoncdgeoups can beOxid'e
+21Vo|b to2
contananyothisgrouplcan he cheecd
3ompromehic deteeto
9 Mass specaomehi'ce detecto
cheomnatogeaphyand mas spechomebhy
rdeol met qul. toe sepouathon and
detchon Fot the output
ot Lc colurmn test
umov Solvnt Them 4he concen thateo
cluat Napoei'sed Bine the sample
tut mars. Apectorchy must be-gucous
nate

You might also like