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HACH DR 5000 USER MANUAL

Quick Links
Stored Programs
Lamp Replacement
Section 8 Troubleshooting
Table of Contents
Table of Contents
Safety information
Use of hazard information
Precautionary labels
Class 1 LASER
Chemical and Biological Safety
Source Lamp Safety
Overview of product
Unpack the instrument
Operating environment
Connection
Cell compartment, Multi Cell Holder, 100 mm Cell Adapter (optional)
mm adapter (optional)
Installation Multi Cell Holder
Beam path
Power the instrument on and off
Language selection
Self-Check
Overview
 Tips for the use of the touch screen
Use of the alphanumeric keypad
Main Menu
Instrument Setup mode
Operator ID
Sample ID
Date and time
Display and sound preferences
Lamp Control
VIS-Lamp and UV-Lamp
Effect caused by the lamp settings on the Measuring Mode
PC and printer
Printer setup
Print data
HACH Data Trans
Password
Password deactivation
Store, recall, send and delete data
The Data Log
Auto/manual data storage
Recall stored data from the data log
Send data from the data log
Delete stored data from the data log
Store, recall, send and delete data from wavelength scan and time
 course
Data storage from wavelength scan or time course
Recall stored data from wavelength scan or time course
Send data from wavelength scan or time course
Delete stored data from wavelength scan or time course
Stored Programs
Select a saved test/method; enter user-specific basic data
Stored program options
Use of program timers
Set the reading mode
Take single wavelength measurements (single reading)
Take single wavelength measurements (continuous readings)
Set the dilution factor
Run a standard adjust
Set the chemical form
Change of the default setting of the chemical form
Run a reagent blank
Analysis of samples
Add stored programs to the favorite programs list
Barcode Programs
Complete a barcode 13 mm test/vial
Select the measuring range
Select the chemical evaluation form
Change of the default setting of the chemical form
 Basic test-specific and sample-specific data settings
Sample blank
Update/edit barcode tests
Manual update of a barcode test
Update an existing barcode test
Program a new test
Upgrade of the instrument software
User Programs
Program a user method
Single wavelength settings
Multi wavelength settings
Calibration settings for single and multi wavelength mode
Store a user program
Additional user-defined parameters and functions
Free programming program type
Measurement process
Enter a new element of a measuring sequence
Enter the calibration formula (evaluation formula)
Enter variables
Save a free programming user program
Select a user program
Add, edit and delete user programs from the favorites list
Add to Favorites
Edit
Delete
Favorite Programs
Recall a favorite program
Delete a favorite program
Standard Addition - monitoring/checking results
Complete a standard addition
Single Wavelength (absorbance, concentration and transmittance
measurements)
Set up single wavelength mode
Take single wavelength measurements (single reading)
Take single wavelength measurements (continuous readings)
Multi-Wavelength mode - measurements at more than one
wavelength
Set the reading mode at different wavelengths
Complete a measurement in the multi wavelength mode
Wavelength Scan mode - recording of absorbance and
transmittance spectrums
Set up the wavelength scan
Wavelength scan reading
Navigation of the wavelength scan graph or a wavelength scan analysis
Work with reference scans
Time course of absorbance/transmittance
Time course setup parameters
Time course scan reading
 Analysis of time course data
Navigation of a time scan or a time scan analysis
System checks
Instrument information
Upgrade of the instrument software
Optical checks
Wavelength check
Noise check
Stray light check
Absorbance check
Drift check
Verification kit
Output checks
Lamp history
Factory service
Service time
Instrument Backup
Cleaning requirements
Spectrophotometer
Display
Cuvettes/sample cells
Lamp replacement
Tungsten lamp replacement
 Deuterium lamp (UV) replacement
Fuse replacement
Filter pad maintenance
Filter pad replacement
 DOC022.53.00654

DR 5000
USER MANUAL

January 2008, Edition 2

© HACH Company, 2007–2008. All rights reserved. Printed in Germany.

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 Table of Contents
Section 1 Specifications ........................................................................................................................................ 7
Section 2 General Information .............................................................................................................................. 9
2.1 Safety information .............................................................................................................................................. 9
2.1.1 Use of hazard information ......................................................................................................................... 9
2.1.2 Precautionary labels ................................................................................................................................. 9
2.1.3 Class 1 LASER ....................................................................................................................................... 10
2.1.4 Chemical and Biological Safety .............................................................................................................. 10
2.1.5 Source Lamp Safety ............................................................................................................................... 10
2.2 Overview of product ......................................................................................................................................... 11
Section 3 Installation ........................................................................................................................................... 13
3.1 Unpack the instrument ..................................................................................................................................... 13
3.2 Operating environment .................................................................................................................................... 13
3.3 Connection ....................................................................................................................................................... 14
3.4 Cell compartment, Multi Cell Holder, 100 mm Cell Adapter (optional) ............................................................. 15
3.4.1 100 mm adapter (optional) ...................................................................................................................... 16
3.4.2 Installation Multi Cell Holder ................................................................................................................... 17
3.5 Beam path ........................................................................................................................................................ 18
Section 4 Start Up ................................................................................................................................................ 19
4.1 Power the instrument on and off ...................................................................................................................... 19
4.2 Language selection .......................................................................................................................................... 19
4.3 Self-Check ....................................................................................................................................................... 19
Section 5 Standard Operations ........................................................................................................................... 21
5.1 Overview .......................................................................................................................................................... 21
5.1.1 Tips for the use of the touch screen ........................................................................................................ 21
5.1.2 Use of the alphanumeric keypad ............................................................................................................ 21
5.1.3 Main Menu .............................................................................................................................................. 22
5.2 Instrument Setup mode .................................................................................................................................... 23
5.2.1 Operator ID ............................................................................................................................................. 23
5.2.2 Sample ID ............................................................................................................................................... 24
5.2.3 Date and time .......................................................................................................................................... 25
5.2.4 Display and sound preferences .............................................................................................................. 25
5.2.5 Lamp Control .......................................................................................................................................... 26
5.2.5.1 VIS-Lamp and UV-Lamp ............................................................................................................. 26
5.2.5.2 Effect caused by the lamp settings on the Measuring Mode ...................................................... 27
5.2.6 PC and printer ......................................................................................................................................... 29
5.2.6.1 Printer setup ............................................................................................................................... 29
5.2.6.2 Print data .................................................................................................................................... 31
5.2.6.3 HACH Data Trans ....................................................................................................................... 31
5.2.7 Password ................................................................................................................................................ 32
5.2.7.1 Password deactivation ................................................................................................................ 33
5.3 Store, recall, send and delete data .................................................................................................................. 35
5.3.1 The Data Log .......................................................................................................................................... 35
5.3.1.1 Auto/manual data storage ........................................................................................................... 35
5.3.1.2 Recall stored data from the data log ........................................................................................... 35
5.3.1.3 Send data from the data log ....................................................................................................... 36
5.3.1.4 Delete stored data from the data log .......................................................................................... 37
5.3.2 Store, recall, send and delete data from wavelength scan and time course ........................................... 38
5.3.2.1 Data storage from wavelength scan or time course ................................................................... 38
5.3.2.2 Recall stored data from wavelength scan or time course ........................................................... 38
5.3.2.3 Send data from wavelength scan or time course ........................................................................ 39
5.3.2.4 Delete stored data from wavelength scan or time course ........................................................... 41

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5.4 Stored Programs .............................................................................................................................................. 42

5.4.1 Select a saved test/method; enter user-specific basic data .................................................................... 42
5.4.2 Stored program options .......................................................................................................................... 42
5.4.3 Use of program timers ............................................................................................................................ 44
5.4.4 Set the reading mode .............................................................................................................................. 44
5.4.4.1 Take single wavelength measurements (single reading) ............................................................ 45
5.4.4.2 Take single wavelength measurements (continuous readings) .................................................. 45
5.4.5 Set the dilution factor .............................................................................................................................. 45
5.4.6 Run a standard adjust ............................................................................................................................. 46
5.4.7 Set the chemical form ............................................................................................................................. 47
5.4.7.1 Change of the default setting of the chemical form .................................................................... 47
5.4.8 Run a reagent blank ................................................................................................................................ 48
5.4.9 Analysis of samples ................................................................................................................................ 49
5.4.10 Add stored programs to the favorite programs list ................................................................................ 49
5.5 Barcode Programs ........................................................................................................................................... 50
5.5.1 Complete a barcode 13 mm test/vial ...................................................................................................... 51
5.5.2 Select the measuring range .................................................................................................................... 51
5.5.3 Select the chemical evaluation form ....................................................................................................... 51
5.5.3.1 Change of the default setting of the chemical form .................................................................... 52
5.5.4 Basic test-specific and sample-specific data settings ............................................................................. 52
5.5.5 Sample blank .......................................................................................................................................... 53
5.5.6 Update/edit barcode tests ....................................................................................................................... 54
5.5.6.1 Manual update of a barcode test ................................................................................................ 54
5.5.6.2 Update an existing barcode test ................................................................................................. 57
5.5.6.3 Program a new test ..................................................................................................................... 58
5.5.7 Upgrade of the instrument software ........................................................................................................ 58
Section 6 Advanced Operations ......................................................................................................................... 61
6.1 User Programs ................................................................................................................................................. 61
6.1.1 Program a user method .......................................................................................................................... 61
6.1.1.1 Single wavelength settings ......................................................................................................... 63
6.1.1.2 Multi wavelength settings ............................................................................................................ 64
6.1.1.3 Calibration settings for single and multi wavelength mode ......................................................... 66
6.1.1.4 Store a user program .................................................................................................................. 70
6.1.1.5 Additional user-defined parameters and functions ..................................................................... 70
6.1.2 Free programming program type ............................................................................................................ 72
6.1.2.1 Measurement process ................................................................................................................ 72
6.1.2.2 Enter a new element of a measuring sequence ......................................................................... 73
6.1.2.3 Enter the calibration formula (evaluation formula) ...................................................................... 75
6.1.2.4 Enter variables ............................................................................................................................ 78
6.1.2.5 Save a free programming user program ..................................................................................... 78
6.1.3 Select a user program ............................................................................................................................. 79
6.1.4 Add, edit and delete user programs from the favorites list ...................................................................... 79
6.1.4.1 Add to Favorites .......................................................................................................................... 80
6.1.4.2 Edit .............................................................................................................................................. 80
6.1.4.3 Delete ......................................................................................................................................... 80
6.2 Favorite Programs ........................................................................................................................................... 81
6.2.1 Recall a favorite program ........................................................................................................................ 81
6.2.2 Delete a favorite program ....................................................................................................................... 81
6.3 Standard Addition – monitoring/checking results ............................................................................................. 82
6.3.1 Complete a standard addition ................................................................................................................. 83

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 Table of Contents

6.4 Single Wavelength (absorbance, concentration and transmittance measurements) ....................................... 87

6.4.1 Set up single wavelength mode .............................................................................................................. 87
6.4.2 Take single wavelength measurements (single reading) ........................................................................ 89
6.4.3 Take single wavelength measurements (continuous readings) .............................................................. 89
6.5 Multi-Wavelength mode – measurements at more than one wavelength ........................................................ 90
6.5.1 Set the reading mode at different wavelengths ....................................................................................... 90
6.5.2 Complete a measurement in the multi wavelength mode ....................................................................... 93
6.6 Wavelength Scan mode – recording of absorbance and transmittance spectrums ......................................... 94
6.6.1 Set up the wavelength scan .................................................................................................................... 94
6.6.2 Wavelength scan reading ....................................................................................................................... 97
6.6.2.1 Navigation of the wavelength scan graph or a wavelength scan analysis .................................. 98
6.6.3 Work with reference scans ...................................................................................................................... 99
6.7 Time course of absorbance/transmittance ..................................................................................................... 101
6.7.1 Time course setup parameters ............................................................................................................. 101
6.7.2 Time course scan reading ..................................................................................................................... 102
6.7.3 Analysis of time course data ................................................................................................................. 103
6.7.3.1 Navigation of a time scan or a time scan analysis .................................................................... 104
6.8 System checks ............................................................................................................................................... 105
6.8.1 Instrument information .......................................................................................................................... 105
6.8.2 Upgrade of the instrument software ...................................................................................................... 106
6.8.3 Optical checks ....................................................................................................................................... 106
6.8.3.1 Wavelength check .................................................................................................................... 107
6.8.3.2 Noise check .............................................................................................................................. 108
6.8.3.3 Stray light check ....................................................................................................................... 110
6.8.3.4 Absorbance check .................................................................................................................... 111
6.8.3.5 Drift check ................................................................................................................................. 112
6.8.3.6 Verification kit ........................................................................................................................... 113
6.8.4 Output checks ....................................................................................................................................... 115
6.8.5 Lamp history ......................................................................................................................................... 115
6.8.6 Factory service ...................................................................................................................................... 116
6.8.7 Service time .......................................................................................................................................... 116
6.8.8 Instrument Backup ................................................................................................................................ 117
Section 7 Maintenance ...................................................................................................................................... 121
7.1 Cleaning requirements ................................................................................................................................... 121
7.1.1 Spectrophotometer ............................................................................................................................... 121
7.1.2 Display .................................................................................................................................................. 121
7.1.3 Cuvettes/sample cells ........................................................................................................................... 121
7.2 Lamp replacement ......................................................................................................................................... 122
7.2.1 Tungsten lamp replacement ................................................................................................................. 122
7.2.2 Deuterium lamp (UV) replacement ....................................................................................................... 123
7.3 Fuse replacement .......................................................................................................................................... 124
7.4 Filter pad maintenance .................................................................................................................................. 125
7.4.1 Filter pad replacement .......................................................................................................................... 125
Section 8 Troubleshooting ................................................................................................................................ 127
Section 9 Replacement Parts ............................................................................................................................ 129
Section 10 Contact Information ........................................................................................................................ 131
Section 11 Limited Warranty ............................................................................................................................. 133
Index .................................................................................................................................................................... 135

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 Table of Contents

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 Section 1 Specifications
Specifications are subject to change without notice.

Performance specifications

Operating mode Transmittance (%), Absorbance and Concentration

Source lamp Gas-filled Tungsten (visible) and Deuterium (UV)

Wavelength range 190–1100 nm

Wavelength accuracy ± 1 nm in Wavelength Range 200–900 nm

Wavelength reproducibility < 0.1 nm

Wavelength resolution 0.1 nm

Wavelength calibration Automatic

Wavelength selection Automatic, based on method selection

Scanning speed 900 nm/min 1 nm steps

Spectral bandwidth 2 nm

Photometric range ± 3.0 Abs in Wavelength Range 200–900 nm

5 mAbs at 0.0–0.5 Abs
Photometric accuracy
1% at 0.50–2.0 Abs
< 0.5%–2 Abs
Photometric linearity < = 1% at > 2 Abs
with neutral glass at 546 nm
KI-solution at 220 nm
Stray light
> 3.3 Abs / < 0.05%
Data storage 1000 measured values (result, date, time, sample ID, user ID)

User programs 50

Physical and environmental specifications

Width 450 mm (17.7 in)

Height 200 mm (7.9 in)

Depth 500 mm (19.7 in)

Weight 15.5 kg (34.2 Ibs)

Operating requirements 10–40 °C (50–104 °F), max. 80% relative humidity (non-condensing)

Storage requirements –25–60 °C (–13–140 °F) max. 80% relative humidity (non-condensing)

Additional technical data

Mains connection 100–240 V~; 50/60 Hz; automatic changeover

Use only shielded cable with max. length of 3 m.
Interfaces 1 x USB type B
2 x USB type A
Water-resistant to
IP 31
Enclosure

Safety class Safety class I

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 Specifications

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 Section 2 General Information

2.1 Safety information

Please read this entire manual before unpacking, setting up or
operating this equipment. Pay attention to all danger, warning and
caution statements. Failure to do so could result in serious injury to
the operator or damage to the equipment.
Make sure that the protection provided by this equipment is not
impaired, do not use or install this equipment in any manner other
than that specified in this manual.

2.1.1 Use of hazard information

DANGER
Indicates a potentially or imminently hazardous situation
which, if not avoided, will result in death or serious injury.

WARNING
Indicates a potentially or imminently hazardous situation
which, if not avoided, could result in death or serious injury.

CAUTION
Indicates a potentially hazardous situation that may result in
minor or moderate injury.

Important Note: Indicates a situation which, if not avoided, may

cause damage to the instrument. Information that requires special
emphasis.

Note: Information that supplements points in the main text.

2.1.2 Precautionary labels

Read all labels and tags attached to the instrument. Personal injury
or damage to the instrument could occur if not observed. A symbol,
if noted on the instrument, will be included with a danger or caution
statement in the manual.

This symbol, if noted on the instrument, references the instruction manual for operation and/or safety information.

This symbol, if noted on the instrument, indicates a hot surface.

Electrical equipment marked with this symbol may not be disposed of in European public disposal systems after
12 August of 2005. In conformity with European local and national regulations (EU Directive 2002/96/EC),
European electrical equipment users must now return old or end-of life equipment to the Producer for disposal at no
charge to the user.
Note: For return for recycling, please contact the equipment producer or supplier for instructions on how to return
end-of-life equipment, producer-supplied electrical accessories and all auxiliary items for proper disposal.

This symbol indicates that the instrument contains a Class 1 LASER device.
LASER CLASS 1 Data: 0.3 mW; λ = 650 nm

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 General Information

2.1.3 Class 1 LASER

A Class 1 LASER is installed in this instrument. Class 1 LASERS
are products where the radiant power of the LASER beam
accessible (the accessible emission) is always below the Maximum
Permissible Exposure value. Therefore, for Class 1 LASERS the
output power is below the level at which it is believed eye damage
will occur. Exposure to the beam of a Class 1 LASER will not result
in eye injury. Class 1 LASERS may therefore be considered safe.
However, Class 1 LASER products may contain LASER systems of
a higher Class but there are adequate engineering control
measures to ensure that access to the beam is not reasonably
likely. Examples of such products include LASER printers and
compact disc players. CDRH assession number 0510555-02.
Data: 0.3 mW; wavelength = 650 nm

2.1.4 Chemical and Biological Safety

DANGER
Potential Chemical/ Biological Exposure Hazards. Handling
chemical samples, standards and reagents can be dangerous.
Users of this product are advised to familiarize themselves
with safety procedures and the correct use of chemicals, and
to carefully read all relevant Material Safety Data Sheets.

Normal operation of this instrument may involve the use of

hazardous chemicals or biologically harmful samples.
• The user must observe all cautionary information printed on the
original solution containers and safety data sheet prior to their
use.
• All waste solutions must be disposed in accordance with local
and national law.
• The type of protective equipment must be selected according to
the concentration and amount of the dangerous substance at
the specific workplace.

2.1.5 Source Lamp Safety

The source lamp operate at high temperatures.
• To avoid a possible electric shock, disconnect the instrument
from the power source before servicing the lamp. Make sure
that the instrument cannot be accidentally switched on again.

CAUTION
The UV lamp generates UV light.
Do not look directly at an operating lamp without wearing UV
protective eye glasses.

Important Note: To prevent a possible burn, allow the lamp to cool

at least 30 minutes before handling.

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 General Information

2.2 Overview of product

The DR 5000 Spectrophotometer is a complete scanning UV/VIS
spectrophotometer with a wavelength range of 190 to 1100 nm. The
instrument comes with a complete set of application programs and
multi-language support.
The DR 5000 Spectrophotometer contains the following application
modes: Stored Programs, User Programs, Favorite Programs,
Single Wavelength Mode, Multi-Wavelength Mode, Wavelength
Scanning Mode and Time Course Mode.
The DR 5000 is used for testing in visible and ultraviolet
wavelengths. A gas-filled tungsten lamp produces light in the visible
spectrum (320 to 1100 nm), and a deuterium lamp available
produces light in the ultraviolet spectrum (190 to 360 nm).
The DR 5000 Spectrophotometer provides digital readouts in direct
concentration units, absorbance, or percent transmittance.
When a user-generated or programmed method is selected, the
on-screen menus and prompts direct the user through the test.
This menu system also can be used to generate reports, statistical
evaluations of generated calibration curves, and to report
instrument diagnostic checks.

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 General Information

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 Section 3 Installation
WARNING
Electrical and Fire Hazards. Use only the provided power
supply. Only qualified personnel should conduct the tasks
described in this section of the manual.

3.1 Unpack the instrument

The DR 5000 Spectrophotometer comes packaged with the
following items:
• DR 5000 Spectrophotometer
• Mains power cable
• Multi Cell Holder
• DR 5000 User Manual
• CD-ROM containing the HACH and HACH LANGE procedures
Note: If any of these items are missing or damaged, contact the
manufacturer or a sales representative immediatly.

3.2 Operating environment

The following conditions are necessary to ensure correct
instrument operation and accurate results:
• Place the instrument firmly on an even surface. Do not push
any objects under the instrument.
• Maintain an ambient temperature of 10 to 40 ºC (50 to 104 ºF)
for proper instrument operation.
• The relative humidity should be less than 80%; moisture should
not condense on the instrument.
• Leave at least a 15 cm (6 in.) clearance at the top and on all
sides for air circulation to avoid overheating of electrical parts.
• Do not operate or store the instrument in extremely dusty,
damp or wet locations.
• Keep the surface of the instrument, the cell compartment and
all accessories clean and dry at all times. Splashes or spills on
and in the instrument should be cleaned up immediately (see
section 7.1 on page 121).
Important Note: Protect the instrument from temperature
extremes, including heaters, direct sunlight and other heat sources.

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 Installation

3.3 Connection
The DR 5000 has three USB interfaces as a standard feature,
located on the back of the instrument (Figure 1).
The USB Type A interfaces are used for communications with a
printer, USB memory stick or keyboard. A USB memory stick is
used to update instrument software.
The USB Type B interface is used for communications with a PC.
The optional Hach Data Trans software (see section 5.2.6.3 on
page 31) must be installed on the PC for this use.
A USB hub may be used to connect several accessories at a time.
Note: USB cables must not be longer than 3 meters (10 feet).

These USB interfaces enable data and graphics to be output to a

Printer and a PC and upgrade of instrument software (see
section 6.8.2 on page 106).

Figure 1 Interfaces
1 USB type A 4 Fuse
2 USB type B 5 Plug in power supply
3 On/Off switch

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 Installation

3.4 Cell compartment, Multi Cell Holder, 100 mm Cell Adapter (optional)

Figure 2 Cell compartment

1 Cell compartment 2 Cell compartment for 13 mm or 16 mm
round cuvettes/vials only

The DR 5000 Spectrophotometer comes equipped with a Multi Cell

Holder (Figure 3 on page 16) which is the Standard Holder,
provided with each instrument. The Multi Cell Holder can
accommodate the following cell types:
• 10 mm square cells
• 20/50 mm rectangular cells
• 1“ round cells
• 1“ square cells
Only one cell type can be used for a measurement. On the top and
bottom of the Multi Cell Holder are a variety of openings to
accommodate different types of cells. Beside each opening is
printed the type of cell for which it is intended.
Optionally there is a 100 mm adapter available.

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 Installation

Figure 3 Multi Cell Holder (Top and Bottom)

1 10 mm square cell 4 1“ round cell
2 20 mm rectangular cell 5 1“ square cell
3 50 mm rectangular cell

3.4.1 100 mm adapter (optional)

Figure 4 100 mm Cell Adapter

1 100 mm rectangular cell

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 Installation

3.4.2 Installation Multi Cell Holder

Assembly of the Multi Cell Holder:

1. Open the cell compartment.

2. Identify the correct opening for the selected cell type in the
Multi Cell Holder.

3. Insert the Multi Cell Holder in the cell compartment in such a

way that the name of the selected cell type can be read directly
and the cell opening is at the front. Secure the holder with two
locking screws (Figure 5).

Figure 5 Installation Multi Cell Holder

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 Installation

3.5 Beam path

Figure 6 shows the beam path of the DR 5000:

Figure 6 Beam path

1 Deuterium lamp 10 Exit slit
2 Lamp selection mirror 11 Cell compartment
3 Tungsten lamp 12 Lens
4 Mirror 13 Round cuvette/vial compartment
5 Grating 14 Measurement element
6 Reference element 15 Monochromator
7 Mirror 16 Mirror
8 Splitter mirror 17 Mirror
9 Filter 18 Entrance slit

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 Section 4 Start Up

4.1 Power the instrument on and off

1. Plug in power supply.

2. Close the empty cell compartment.

3. Turn the instrument on by pressing the power switch on the

back.

Note: Do not turn the instrument off and on in rapid succession. Always
wait about 20 seconds before turning the instrument on again, otherwise
the electronic and mechanical systems will be damaged.

4.2 Language selection

The spectrophotometer software includes several language

options. The first time the instrument is turned on, the language
selection screen will appear.

1. Select the desired language.

2. Press OK to confirm the language selection. The self check will

start automatically.

Changing the language setting

The instrument functions in the selected language until the option is
changed.

1. While turning the instrument on, touch the screen at any point
until the list for selecting a language appears (about
30 seconds).

2. Select the required language.

3. Press OK to confirm. The test program subsequently starts

automatically.

4.3 Self-Check

Each time the instrument is powered up, a series of diagnostic tests

are performed automatically to ensure operation of major system
components.
This procedure, which takes approximately two minutes, checks the
system, lamp, filter adjustment, wavelength calibration and voltage.
Each test which functions correctly is confirmed with a check mark.
The Main Menu is displayed when power up diagnostics are
completed.
Note: Further error messages during self check, see Section 8 on page
127.

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 Start Up

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 Section 5 Standard Operations

5.1 Overview
5.1.1 Tips for the use of the touch screen
The entire screen is touch-activated. To make a selection, press the
screen with a fingernail, fingertip, pencil eraser or a stylus. Do not
press the screen with a sharp object, such as the tip of a ball
point pen.
• Do not place anything on top of the screen, to prevent damage
or scratching on the screen.
• Press keys, words or icons to select them.
• Use scroll bars to move up and down long lists very quickly.
Press and hold the scroll bar, then move up or down to move
through the list.
• Highlight an item from a list by pressing it once. When the item
has been successfully selected, it will be displayed as reversed
text (light text on a dark background).

5.1.2 Use of the alphanumeric keypad

This display is used to enter letters, numbers and symbols as

needed when programming the instrument. Unavailable options are
disabled (grayed out). The icons on the right and left of the screen
are described in Table 1.
The central keypad changes to reflect the chosen entry mode.
Press a key repeatedly until the desired character appears on the
screen. A space can be entered by using the underscore on the
YZ_ key.
Note: A USB keyboard (with US keyboard layout) or a USB Barcode
handset scanner can be used for input (see Section 9 on page 129).

Table 1 Alphanumeric keypad

Icon / key Description Function
When entering alphabetic characters (ex. user-entered units), this key allows to
ABC/abc Alphabetic
toggle between upper and lower case letters.
#% Symbols Punctuation, symbols and numerical sub- and superscripts may be entered.

123 Numeric For entering regular numbers.

CE Clear Entry Clear the entry.

Moves back one position. This deletes the character previously entered in the new
Left Arrow Backspace
position.
Moves to the next space in an entry when two adjacent characters occur on the
Right Arrow Advance
same key.

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5.1.3 Main Menu

A variety of modes may be selected from the Main Menu. The

following table briefly describes each menu option.

Table 2 Main Menu options

Option Function

Stored programs are pre-programmed methods that make use of HACH reagents and LANGE
Stored Programs (HACH cuvette tests and pipette tests.
LANGE Programs) The DR 5000 Procedures Manual contains illustrated, step-by-step procedures for analyses
using HACH programs. The working procedures for LANGE tests are included in the test packs.
User programs make "made to measure analysis" possible:
–Users can program methods they have developed themselves
User Programs
–Existing HACH and LANGE methods can be stored as user programs. The LANGE tests can
then be modified to suit the user's requirements.
Favorite Programs List of methods/tests created by the user to suit his own requirements.
Single wavelength measurements are:
Absorbance measurements: The light absorbed by the sample is measured in absorbance
units.
Single Wavelength Transmittance measurements (%): The percentage of the light that passes through the sample
and reaches the detector is measured.
Concentration measurements: A concentration factor can be entered to enable the measured
absorbance values to be converted into concentration values.
In the multi-wavelength mode, absorbance (Abs) or percentage transmittance (%T) is measured
Multi Wavelength at up to four wavelengths and absorbance differences and absorbance relationships are
calculated. Simple conversions into concentrations can also be carried out.
A wavelength scan shows how the light from a sample is absorbed over a defined wavelength
spectrum.This function can be used to determine the wavelength at which the maximum
Wavelength Scan
absorbance value can be measured.The absorbance behavior is displayed graphically during the
scan.
Time Course The time scan records the absorbance or % transmittance at a wavelength over a defined time.
The system checks menu offers a number of options, including optical checks, output checks,
System Checks
lamp history, instrument update, service time and instrument backup.
Recall Data Stored data can be recalled, filtered, sent and deleted.
In this mode, user-specific or method-specific settings can be entered: Operator-ID, Sample-ID,
Instrument Setup
Date & Time, Display & Sound, Lamp Control, PC & Printer, Password, and Module Options.

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5.2 Instrument Setup mode

1. Select Instrument Setup in the Main Menu.

A selection of functions appears in order to configure the functions

of the instrument.
This display appears when the multi-cell holder is installed or the
13 mm round cuvette/vial compartment is used. If the imstrument
recognizes a Sipper module or a Peltier module the Carousel
Options key is replaced by Sipper Options or Peltier Options.
Optional modules for the DR5000:
• Carousel Holder (Sample Changer)
• Pour-Thru Cell module
• Sipper module
• Peltier module (Temperature control module)
Refer to the corresponding manuals/instruction sheets for more
information.

5.2.1 Operator ID
Use this option to enter up to 30 sets of operator initials (up to
5 characters each) into the instrument. This feature helps record
which operator measured each sample.

1. Press Operator ID in the Instrument Setup.

2. Press New to enter a new Operator ID.

3. Use the alphanumeric keypad to enter a new Operator ID.

4. Press OK to confirm.

5. The display shows the chosen Operator ID.

6. Press OK. The instrument will return to the Instrument Setup

screen and show the selected operator identifier.

7. The chosen Operator ID is activated.

Note: Press Delete to remove an Operator ID from the list.

Note: Alternatively, enter or change an Operator ID in measurement mode.

In the results screen, press Options>More>Instrument Setup or if an
Operator ID is already assigned, select the "Operator ID symbol
immediately in the results screen.

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5.2.2 Sample ID
Use this option to enter up to 30 Sample Identification tags (up to
13 characters each) into the instrument. Sample IDs can be used to
specify the sample location or other sample specific information.

1. Press Sample ID in the Instrument Setup.

2. Press New to enter a new Sample ID.

3. Use the alphanumeric keypad to enter a new Sample ID.

Note: If a USB Barcode handset scanner ( see Section 9 on page 129)

is connected, Sample IDs can also be scanned.

4. Press OK to confirm.

5. To number the Sample IDs sequentially (e.g. Inflow (01 etc.)),

select Add Number.

• Use the arrow keys to specify the first number of the

sequence.
• Use the key between the arrow keys to enter the first
number of the sequence using the alphanumeric keypad.

6. Press OK to return to "Instrument Setup".

7. The Sample ID is activated. Each Sample ID is automatically

numbered in ascending order after a measurement. The
number is shown in parentheses behind the Sample ID.

To remove a Sample ID, highlight the ID and press Delete.

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There are two ways to enter or change a Sample ID in the

Measurement Mode:

Option 1
In the result display, press Options>More>Instrument Setup. If a
Sample ID is already assigned, select the Sample ID symbol in the
result display.

Option 2
With an USB keyboard (with US keyboard layout) or a scanner a
Sample ID can be directly entered in the result display.

5.2.3 Date and time

1. Press Date & Time in the Instrument Setup.

2. The date and time are subdivided over a number of fields.

Press the appropriate field and use the arrow keys to change
the value.

3. Press OK to confirm. The instrument will return to

Instrument Setup.

5.2.4 Display and sound preferences

1. Press Display & Sound in the Instrument Setup.

The following options will be displayed:

• Display Contrast—Adjusts the display brightness to suit
lighting conditions.
• Screen touch—Activates//Deactivates a short beep each time
the screen is pressed (Default:off).
• Reading done—Activates/Deactivates a sound when a
reading is complete (Default: short beep every time a reading is
complete).
• Timer—Adjusts the length of the timer sound. Select Short or
Long. Long beeps are recommended for noisy environments.

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2. Select Long to change the number of audio signals.

Use the alphanumeric keypad to enter/specify the number of

audio signals (4–25).

Note: A high number of audio signals increases the duration of the

tones and a small number of audio signals reduces the duration of the
tones.

3. Press OK to confirm. The selected number of the audio signals

sounds as a corresponding acoustic signal.

4. Press OK to confirm. The instrument will return to

Instrument Setup.

5.2.5 Lamp Control

The tungsten lamp produces light in the visible spectrum 320 to
1100 nm.
The deuterium lamp (UV-lamp) available produces light in the
ultraviolet spectrum 190 to 360 nm.
In the overlap zone from 320 to 360 nm, either the deuterium lamp
(UV-lamp) or the tungsten lamp can be used for measurements.
The lifetime of the lamps is influenced
• by on-off operation and the length of time in use.
• Typical use is to turn the instrument on for the entire 8–10 hour
shift, and then off until the next day.
Note: In general, avoid on/off cycles of the lamp; this shortens the lamp’s
life span. For maximum life, turn the lamp off only if it will remain off for at
least 4–5 hours.

The lamp switches on automatically

• if a lamp is needed for the selected program
• if the instrument is operating inside the lamp’s spectrum

5.2.5.1 VIS-Lamp and UV-Lamp

1. Press Lamp Control in the Instrument Setup.

2. Select On to switch on the VIS-Lamp and/or UV-Lamp.

3. Select Save: in order to define a time interval for the burning

time of the VIS lamp and/or UV lamp.

4. Press the field below Save to select the lamp burning time.

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5. Select the length of time the lamp will be switched on.

Note: After this period of time the lamp automatically turns off, after no
measurement has been made outside the corresponding range (UV/VIS).

6. Touch OK to confirm.

7. Touch UV Switch key to select the wavelength value between

320 nm and 360 nm, at which the instrument changes from the
visible to UV source.

8. Use the alphanumeric keypad to enter the maximum

wavelength for UV operation.

9. Touch OK to confirm and return to “Lamp Control“.

10. Touch OK to confirm and return to “Instrument Setup“.

Note: The selected program that requires a lamp has highest priority. If the
lamp in the Lamp Control was turned off, the lamp will be turned on
automatically if it is needed for the stored program.

5.2.5.2 Effect caused by the lamp settings on the Measuring Mode

UV-Lamp switches on manually. The icon ”UV Lamp“ appears in the

display and flashes.
The instrument will display ”Lamp Warmup...“. This takes about
3 minutes.

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When the lamp is warmed up and ready, the UV icon stops flashing.
Note: If both lamps are On, the icon UV-VIS is displayed in the selected
Measurement Mode.

Note: Alternatively you can touch the UV- or VIS-Lamp icon on the
measurement screen to change the actual setting.

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5.2.6 PC and printer

The instrument is provided with 3 USB interfaces, which are located
on the back of the instrument (see Figure 1 on page 14). These
interfaces can be used for exporting data and graphics to a printer,
updating data and for data communication to a personal computer.
These interfaces can be used for the connection of a USB stick, an
external USB keyboard or a USB Barcode handset scanner.
Note: A USB hub may be used to connect several accessories at a time.

A USB memory stick is used to upgrade data and software, see

section 6.8.2 on page 106.
Important Note: A screened USB cable must not be longer than
3 m!

Table 3 USB connector

USB Interfaces Description
This USB interface is only intended to connect the instrument to a PC (with installation of the
USB (Type B)
HACH Data Trans Software).
These USB ports can be used to connect a printer, a USB memory stick, a barcode scanner or a
USB (Type A)
keyboard.

1. Press PC & Printer in the Instrument Setup.

A list with information about the connections opens.

5.2.6.1 Printer setup

For reasons of compatibility, the printer language must be

HP PCL 3.

2. Press Printer.

3. Press Setup to display the Printer Setup screen.

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Printer Setup:
• Resolution: Print quality
• Paper: Paper size
Note: If an optional Thermal Printer is connected, the function "Auto Send"
on/off is available.

4. Select Auto-Send: On to send all measured data automatically

to the Thermal printer.

Note: The option Auto-Send is not available for any other printer (e.g. ink
jet printer).

5. Press Resolution to select the print quality.

Select between

• 100 dpi
• 150 dpi and
• 300 dpi

6. Press OK to confirm.

Note: Press OK again to return to the PC & Printer menu.

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7. Press Paper to select the paper size.

Select between

• Letter
• Legal
• Executive
• A4

8. Press OK to confirm.

Note: Press OK again to return to the PC & Printer menu.

5.2.6.2 Print data

1. Press Recall Data in the Main Menu.

2. Select the data source, where the data to be printed are stored.

3. A list is displayed. Data can be filtered. For more information

see section 5.3.1.2 on page 35.

4. Press the PC & Printer icon to send the data (table, curve)
immediately to the printer.

5. Highlight Single point or Filtered data or All data and press

OK to confirm.

Sending Data... is displayed until the data have been printed.

5.2.6.3 HACH Data Trans

The optional HACH Data Trans software must be installed on the
PC for the subsequent to process for measurement data.

1. Press PC & Printer in the Instrument Setup.

2. Select PC.

3. Press Setup to display the PC Setup screen.

For further installation instructions, refer to the HACH Data

Trans user manual.

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4. Select Auto-Send: On to send all measured data automatically

to the PC.

Note: If Auto-Send: Off is selected, the PC & Printer icon must be

pressed, in order to send data to the PC.

Note: The remote function is only for monitoring the data transfer.

5.2.7 Password
The Password menu contains a variety of security settings to
control access to various functions. For example, prevent
unauthorized changes to stored programs or instrument
configurations.

1. Press Password in the Instrument Setup menu.

2. In order to highlight the Security List assign a password. Press

Set Password.

3. Use the alphanumeric keypad to enter a new Password (up to

10 characters each) and press OK to confirm.

The access to the Security List is activated.

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4. Press Security List to lock various functions for unauthorized

users.

5. Highlight the desired functions to control.

6. Confirm the Security List with OK to return to the Password

menu.

7. Press On to highlight the new settings of the Security List.

8. Enter the new Password again to confirm.

9. Press OK to return to Instrument Setup.

Note: The alphanumeric keypad to the Password inquiry appears when a

user tries to reach a locked setting.

5.2.7.1 Password deactivation

1. Press Password in the Instrument Setup.

2. Use the alphanumeric keypad to enter the former Password

and press OK to confirm.

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3. Press Off to deactivate the settings of the Security List.

4. Press OK to return to Instrument Setup.

Note: Use this function to delete the former Password or to enter a new
one.

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5.3 Store, recall, send and delete data

5.3.1 The Data Log
The Data Log will store up to 1000 readings taken in the modes:
Stored Programs, User Programs, Favorite Programs, Single
Wavelength and Multi Wavelength. A complete record of the
analysis is stored, including the Date, Time, Results, Sample ID,
and Operator ID.

5.3.1.1 Auto/manual data storage

The data storage parameter indicates whether data are to be stored
automatically or manually (in which case the user has to decide
which data to store).

1. Press Store: On/Off in the Options menu.

• With the Store On setting, all measurement data are stored

automatically.
• With the Store Off setting, no measurement data are
stored. However, this setting can be changed to Store On
in the result display through configuration. The reading
currently shown in the display is then stored.
Note: When the instrument's memory (data log) is full, the oldest data are
automatically deleted allowing the new data to be stored.

5.3.1.2 Recall stored data from the data log

1. Press Recall Data in the Main Menu.

2. Press Data Log.

A listing of the stored data is displayed.

3. Press Filter: On/Off.

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4. The function Filter Settings is used to search for specific

items.

5. Highlight On to turn on the filters to select data by

• Sample ID
• Operator ID
• Start Date
• Parameter

or any combination of the four.

6. Press OK to confirm the selection. The chosen items are listed.

7. Press View Details to get more information.

5.3.1.3 Send data from the data log

Data is sent from the data log as CSV (Comma Separated Value)
files through a USB memory stick to a file named DATALOG. The
file can then be processed using a spreadsheet program. The file
name will be formatted as:
DLYear_Month_Day_Hour_Minute_Second. CSV.
To send data to a Printer, see section 5.2.6.2 on page 31.

1. Plug in the USB device (Figure 1 on page 14).

2. Press Recall Data from the Main Menu. Press Options and
then the PC & Printer icon

3. Select the data to send to the memory stick and press OK.

Note: The number in parenthesis is the total number of data sets

assigned to this selection.

To send measurement data to a PC:

The optional HACH Data Trans software must be installed on the
PC, see section 5.2.6.3 on page 31.

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5.3.1.4 Delete stored data from the data log

1. Press Recall Data in the Main Menu.

2. Press Data Log>Options>Delete.

3. Highlight Single Point or Filtered data or All data and press

OK to confirm.

Note: The number in parentheses is the total number of data sets

assigned to this selection.

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5.3.2 Store, recall, send and delete data from wavelength scan and time course
The instrument can store 20 Wavelength Scans and 20 Time
Course Data sets.The data can be stored manually at the user's
discretion after viewing the data.

5.3.2.1 Data storage from wavelength scan or time course

1. Press the Store icon in the Options menu after a reading is

taken.

The Store Data list will be displayed.

2. Press Store to save the current scan to the highlighted

numbered line. A scan can also be overwritten.

5.3.2.2 Recall stored data from wavelength scan or time course

1. Press Recall Data in the Main Menu.

a. Select Wavelength Scan or Time Course to recall data.

b. If a program is already in progress, press

Options>More>Recall Data.

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2. Press Graph to look at details.

Note: Press View Summary to return to the Recall Data list.

3. Press Table to look at details.

Note: Press View Summary to return to the Recall Data list.

5.3.2.3 Send data from wavelength scan or time course

There are two ways to recall sent data to a USB memory stick,
printer or PC with Hach Data Trans.

Option 1:

1. Press Recall Data in the Main Menu and then Wavelength

Scan or Time Course.

2. Press Options and then the PC & Printer icon to send the
data to a USB memory stick, to a printer or to a PC with Hach
Data Trans.

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• When a printer is connected, select how to send the data to

the printer (graph, table or both graph and table).
• When a USB memory stick is connected, the files will be
automatically sent as CSV files (Comma Separated Value)
to a file ”WLData“ (Wavelength Scan Data) or ”TCData“
(Time Course Data) to the USB memory stick.

The file name will be formatted as: ”ScanData_X.csv“

(Wavelength Scan Data) or ”TCData_X.csv” (Time Course
Data).
X = number of scans (1–20)

For further processing use a spreadsheet program.

Note: The advice ”Data already exist. Overwrite?“ appears when the files
were already stored. Press OK to overwrite the stored data.

Option 2:

1. Press Wavelength Scan or Time Course and then

Options>More>Send Data to send the data to a USB memory
stick or to a printer.

• When a printer is connected, select how to send the data to

the printer (graph, table or both graph and table).
• When a USB memory stick is connected, the files will be
automatically sent as CSV files (Comma Separated Value)
to a file ”WLData“ (Wavelength Scan Data) or ”TCData“
(Time Course Data).

The file name will be formatted as:

”ScanData_Year_Month_Day_Hour_Minute_Second.CSV“
(Wavelength Scan Data) or
The file name will be formatted as:
”TCYear_Month_Day_Hour_Minute_Second.CSV“ (Time
Course Data).

For further processing use a spreadsheet program.

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5.3.2.4 Delete stored data from wavelength scan or time course

1. Press Recall Data from the Main Menu and then Wavelength
Scan or Time Course or Options>More>Recall Data.

A listing of the stored data is displayed.

2. Highlight any data to delete.

3. Press Delete in the Options menu and press OK to confirm.

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5.4 Stored Programs

The instrument contains more than 200 programmed procedures.
They can be accessed through the Stored Programs menu.

5.4.1 Select a saved test/method; enter user-specific basic data

4. Press Stored Programs in the Main Menu to view an

alphabetical list of stored programs with program numbers.

The Stored Programs list will appear.

5. Highlight the required test.

Note: Select the program number by name or use the arrow keys to
scroll through the list quickly and highlight the program or press
Select by number to search for a specific program number. Use the
alphanumeric keypad to enter the test number and press OK.

6. Press Start to run the program. After a program is selected, the

screen for that parameter will appear.

Note: All corresponding data (wavelength, factors and constants) are

already preset.

7. Follow the chemical procedures described in the corresponding

Procedures Manual.

5.4.2 Stored program options

1. From the Main Menu, select Stored Programs. Select the

necessary method and press Start.

2. Press Options for Parameter Setup. Refer to Table 4 for stored

program descriptions.

Table 4 Stored programs options

Options Description

More For further Options

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Table 4 Stored programs options (continued)

Options Description
With the Store On setting, all measurement data are stored automatically. With the Store Off
Store Off/On
setting, no measurement data are stored.
% Trans/Conc/Abs To switch to % transmittance, concentration or absorbance readings

Send Data icon /

To send Data to a printer, computer or USB memory stick (Type A)
Send Data
This functions as a stopwatch. It helps to ensure that the steps of an analysis are correctly timed
(e.g. reaction times, wait times, etc., can be exactly specified). When the specified time has
Timer icon
elapsed, an acoustic signal is emitted. The use of the timer has no influence on the measurement
program.
Single Reading Mode: A reading is only displayed after a measurement has been carried out
(press Read; standard setting) (see section 5.4.4.1 on page 45).
Reading Mode
Continuous Reading Mode: After the zero measurement, all readings are displayed
automatically and continuously (see section 5.4.4.2 on page 45).
A corrective dilution factor can be entered in order to take account of certain properties.
The number entered at the dilution factor prompt will be multiplied by the result to compensate for
Dilution Factor Off/On the adjustment. For example, if the sample has been diluted by a factor of 2, enter 2. The default
setting of the dilution factor is turned off.
Note: When a dilution is in effect, the dilution icon will appear on the display.
This enables the accuracy of the measurements to be checked. The (working) procedure for a
Standard Addition
test parameter contains a detailed explanation of how to use this function.
The (working) procedure for a test parameter indicates whether a standard adjustment is
Standard Adjust
necessary and, if so, how to proceed.
Chemical Form Some of the stored tests/methods allow to select the chemical form and the measuring range.
Some of the stored tests/methods include the "Reagent Blank" function. This enables the reagent
blank value to be added to or subtracted from, the subsequent readings. The reagent blank value
Reagent Blank shifts the calibration curve along the y-axis, without changing the shape or gradient of the curve.
The effect corresponds to a y-axis intercept of the calibration straight line. This is made clear by
the following equation: Concentration = [(Conc. factor) * Abs] – (reagent blank value).
Save as User Program To store the selected parameters as a User Program, see section 6.1 on page 61.
Call up saved measurement data, wavelength scans or time courses, see section 5.3 on
Recall Data
page 35.
Instrument Setup Basic data of the instrument, see section 5.2 on page 23.

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5.4.3 Use of program timers

Some procedures do not require the use of timers. Other
procedures require several timers. These timers are
pre-programmed into each Stored Program, along with a
description of the activity to be performed during the timed period.

1. Press the Timer icon on the display.

2. Press OK to start the first timer.

The timer will count down on the screen.

3. To start the next timed activity for the Stored Program, press
the Timer icon and OK.

Note: Press Close to view the measurement screen while the timer is
running. The time will be shown left side bottom instead of the date.

Note: Press Cancel to stop the timer midway through the countdown.

Note: The Timer will beep when the end of the running time is reached.

A general purpose timer is also available in many programs. When

the timer icon is visible, press the icon and select General Timer.
A new screen will appear. Enter the length of the timed interval and
press OK to start the timer. The timer will beep when the timed
interval ends.

5.4.4 Set the reading mode

1. To highlight the required mode, press Reading Mode.

2. Select the required mode, then press OK, then Return to return
to the result display.

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5.4.4.1 Take single wavelength measurements (single reading)

1. Insert the blank cuvette into the cuvette/sample cell holder.

Press Zero.

Note: The Read key is only active after the zero measurement has
been carried out.

2. Insert the sample cuvette into the cuvette/sample cell holder.

Press Read.

Note: For data storage, see section 5.3.2 on page 38.

5.4.4.2 Take single wavelength measurements (continuous readings)

1. Insert the blank cuvette into the cuvette/sample cell holder.

Press Zero.

Note: In the reading mode ”Continuous“ only the Zero key is shown to
start the reading. The reading sequence is started automatically.

2. Insert the sample cuvette into the cuvette/sample cell holder.

3. Press Options and then the Store icon to store the displayed
data in the Data Log.

Note: For data storage, see section 5.3.2 on page 38.

5.4.5 Set the dilution factor

The Dilution Factor function is used to calculate the original
concentration of a sample that has been diluted by a known ratio.
For example, dilution factor can bring the analyzed concentration
within the test range.

1. Press Options>More…>Dilution Factor.

The number entered at the dilution factor prompt will be

multiplied by the result to compensate for the adjustment.

For example, if the sample has been diluted by a factor of 2,

enter 2. The default setting of the dilution factor is turned off.

2. Press OK to confirm.

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3. Press Return to return to the result display.

4. Confirm ’Store present reading again?’ to save the current

measured value with the dilution factor.

Note: When a dilution is in effect, the dilution icon will appear on the
display.

Note: If using undiluted samples, set the dilution factor off again.

5.4.6 Run a standard adjust

The Standard Adjust function allows the calibration curve for a
stored program to be adjusted based on analysis of a known
standard solution. The Accuracy Check section of written
procedures often suggests a standard solution concentration for
this purpose.
Read a standard before setting Standard Adjust to On.

1. Follow the entire procedure, using a known standard for the

sample.

2. After reading the concentration, press

Options>More>Standard Adjust.

3. If Standard Adjust is set to Off, turn it On.

The Current Reading will show the concentration. The box on

the right will show the default standard value for the test, as
mentioned in the procedure.

4. If the measurement used a standard concentration that is

different from the one displayed in the box, press the box on the
right to enter a different standard value and enter the new
value. Press OK to confirm.

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5. Press Adjust to enable the Standard Adjust. The Standard

Adjust icon will appear.

Note: The adjustment must be within certain limits, which vary with
each program. The allowable percentage is shown after "Adjustment“.

Note: When a Standard Adjust is in effect, the Standard Adjust icon

will appear on the display.

5.4.7 Set the chemical form

Some Stored Programs allow a variety of chemical forms to
be selected.
Press the unit (e.g. mg/L) or the chemical representation of the
evaluation form (e.g. Al3+). A list of available evaluation forms is
displayed. Select the required form by pressing the corresponding
entry in the list.
Note: When the program is exited, the evaluation form reverts to the
standard setting.

An alternative way of changing the standard setting:

1. Press Options>More>Chemical Form.

2. Select the Chemical Form.

Note: The stoichiometric conversion of the measurement result is

carried out automatically.

Note: The selected Chemical Form will appear on the display. Test
results will be calculated and displayed in this chemical form.

5.4.7.1 Change of the default setting of the chemical form

1. Insert the sample cuvette or blank (depending on the working

procedure) into the cell compartment.

2. In the result display, press Options>More>Chemical Form.

3. A list of available evaluation forms appears. Select the new

default setting.

4. Press Save as Default.

The current result and all further measurements will be displayed in

the new chemical form.

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5.4.8 Run a reagent blank

Some of the stored tests/methods include the "Reagent Blank"
function. This enables the reagent blank value to be measured and
then taken into account in calculating the measurement result.

Measurement/analysis of a reagent blank:

1. Prepare the test/method in accordance with the (working)

procedure. Instead of a sample, deionized water is used to
determine the reagent blank value.

2. Select the test. If required by the (working) procedure, insert

the cell with deionized water into the cell compartment. Press
Zero.

3. Insert the prepared sample cell into the cell compartment.

Press Read. The result is displayed.

4. Press Options>More>Reagent Blank.

5. Press On to highlight the Reagent Blank function.

6. The concentration shown on the key is the measured value of

the reagent blank. To use this value for more analyses of this
parameter, press OK.

To enter a previously recorded reagent blank value, press the key

and use the alphanumeric keypad.

7. Press OK.

Note: The Reagent Blank function is deactivated when the measurement

program is left. To use the same blank value later for other tests using the
same reagent lot, enter the value per step .

Note: The results calculated using the reagent blank value must lie within
the limits of the measuring range of the test/method.

Note: The reagent blank icon is shown in the result display (see arrow)
when the function is active.

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5.4.9 Analysis of samples

1. Press Stored Programs and select a program.

2. Insert the blank cuvette/cell into the cuvette/sample cell holder.

3. Press Zero.

4. Remove blank cuvette/cell and insert sample cuvette/cell into

the cell compartment.

5. Press Read. The result will be displayed.

6. For data storage, see section 5.3.2 on page 38.

5.4.10 Add stored programs to the favorite programs list

The Favorites menu simplifies test selection by creating a list of the
most frequently used tests from the Stored Programs and
User Programs.

1. Press Stored Programs in the Main Menu. The Stored

Programs list will appear.

2. Highlight the selection by pressing it or Select by Number to

search for the program by number.

3. Press Add to Favorites and press OK to confirm.

The program can now be selected from Favorite Programs

menu in the Main Menu.

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5.5 Barcode Programs

A special barcode reader in cell compartment #1 automatically
reads the barcode on the 13 mm cuvette/vial as the cuvette/vial
completes a single rotation. The instrument uses the barcode
identification to automatically set the correct wavelength for the
analysis and calculates the result immediately with the help of the
stored factors.
In addition, measured values are recorded at 10 different positions
during a rotation. A special outlier-elimination program is run and
then the average of the measured values is calculated. Cuvette/vial
errors and soiling are recognized and highly precise results are
obtained.

Figure 7 Cell compartment #1

1 Cell compartment #1 for 13 mm barcode cuvettes/or 16 mm vials

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5.5.1 Complete a barcode 13 mm test/vial

Note: The cell compartment must be closed before a measurement is

made!

1. The instrument needs to be at the Main Menu.

2. Prepare the barcode 13 mm cuvette/vial in accordance with the

working procedure and insert the cuvette/vial in cell
compartment #1 and close the cell compartment.

When a coded cuvette/vial is placed in cell compartment #1

(Figure 7), the corresponding measurement program is
automatically activated in the Main Menu.

The measurement is started automatically and the results are

displayed.
To evaluate other cuvette/vial tests and other parameters, insert the
prepared cuvette/vial into the cell compartment and read the result.

5.5.2 Select the measuring range

Some tests can be used for different measuring ranges. After the
sample cuvette/vial has been inserted, a list of the different
measuring ranges is displayed.
Select the required measuring range by highlighting the appropriate
line.
Press Start Permanent if this measuring range is to apply to all
subsequent measurements.

Changing the standard setting

In the result display, press Options>More>Permanent: On. The
key will change to Permanent: Off.

5.5.3 Select the chemical evaluation form

The chemical form of the test result of a number of parameters can
be selected individually.
In the result display, press the unit (e.g. mg/L) or the chemical
representation of the evaluation form (e.g. PO43––P). A list of
possible evaluation forms is displayed, from which the required
form can be selected. Press OK to confirm.
Another way of changing the standard setting is:

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1. In the result display, press Options>More>Chemical Form.

A list of available evaluation forms appears.

2. Select the required chemical form and press OK to confirm.

Note: The selected chemical form is displayed, but does not become
the default. To change the default, see section 5.5.3.1.

5.5.3.1 Change of the default setting of the chemical form

1. Insert the blank or sample cuvette/vial (depending on the

working procedure) into the cell compartment and close the cell
compartment.

2. In the result display, press Options>More>Chemical Form.

3. A list of available evaluation forms appear. Select the new

default setting.

4. Press Save as Default.

The current result and all further measurements will be displayed in

the new chemical form.

5.5.4 Basic test-specific and sample-specific data settings

Press Options to change test or sample specific settings.

Table 5 Barcode program options

Options Description

More For further Options

With the Store On setting, all measurement data are stored automatically. With the Store Off
Store Off/On
setting, no measurement data are stored.
Abs % Trans To switch to % transmittance or absorbance readings

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Table 5 Barcode program options (continued)

Options Description

Send Data icon /

To send data to a printer, computer or USB memory stick (Type A)
Send Data
This functions as a stopwatch. It helps to ensure that the steps of an analysis are correctly timed
(e.g. reaction times, wait times, etc., can be exactly specified). When the specified time has
Timer icon
elapsed, an acoustic signal is emitted. The use of the timer has no influence on the measurement
program.
A corrective dilution factor can be entered in order to take account of certain properties.
The number entered at the dilution factor prompt will be multiplied by the result to compensate for
the adjustment. For example, if the sample has been diluted by a factor of 2, enter 2. The default
Dilution Factor Off/On
setting of the dilution factor is turned off.
Note: When a dilution is in effect, the dilution icon will appear on the display.
Note: If undiluted samples are used, set the dilution factor off.
Chemical Form Some of the stored tests/methods allow to select the chemical form and the measuring range.

Edit To modify an existing program

Save as User Program To store the selected parameters as a User Program, see section 6.1 on page 61.
Call up saved measurement data, wavelength scans or time courses, see section 5.3 on
Recall Data
page 35.
Instrument Setup Basic data of the instrument, see section 5.2 on page 23.

5.5.5 Sample blank

Turbidity and color in the sample matrix can falsify the results of a
photometric analysis. The interference factors come from the
sample or are created by reactions with the reagents.
The influence of turbidity and/or color can be eliminated or reduced
by taking a sample blank reading.
In the barcode mode, a special cuvette/vial (TNT919) containing
the sample blank is placed in cell compartment #1 after the sample
reading has been taken and is automatically measured. The
sample reading is then corrected by adding or subtracting the blank
value. The final result is displayed, with the message "After blank
value corr.".
Some barcode tests do not require a sample blank value to be
determined, as turbidity and color are dealt with during the test

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procedure. Other barcode tests use the reagent test vials to

determine the sample blank. Please refer to the Hach procedure for
method-specific instructions.

5.5.6 Update/edit barcode tests

5.5.6.1 Manual update of a barcode test

Using the data provided in the barcode, the instrument

automatically sets the measurement wavelength and factors. If a
discrepancy is detected between the barcode data and the stored
data or a new test is identified, the instrument requests an update.
Data types are described in Table 6.

Table 6 Program update options

Program point Description

Name Name of the analysis parameter

Version An abbreviation assigned by the user or the version number, etc. is entered here
Exact definition of the test: the number of wavelengths at which measurements are carried out,
Measurement process the number of absorbance measurements needed, the keys to be used, any waiting periods
between measurements, etc.
Formula Definition of the evaluation formulas with which the test result is calculated
The number of variables shown in the display depends on the definition of the measurement
Variables process and the formulas. Input of the numerical values of the wavelengths, factors, constants,
etc.
This function can be used to enter abbreviations and defined times for up to four timers. Highlight
the appropriate line and press Edit.
Timer 1, Timer 2,
The timers are activated or deactivated with the control boxes on the left of the display. In the
Timer 3, Timer 4
next column, a selection can be made from a list of names that describe the corresponding work
step. In the third column, the times for each active timer are entered.

Enter the Name

Check the working procedure in advance to determine whether this
point needs to be changed.

1. Highlight the line containing Name and press Edit. Use the
alphanumeric keypad to enter the name indicated in the
working procedure.

2. Press OK to confirm.

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Enter the Version

1. Highlight the line containing Version and press Edit. Use the
alphanumeric keypad to enter the name indicated in the
working procedure.

2. Press OK to confirm.

Enter the Measurement Process

For detailed information on entering the measurement process, see
section 6.1.2 on page 72.
Check the working procedure in advance to determine whether this
point needs to be changed.

1. Highlight the Measurement Process line in the data overview

and press Edit.

2. Press Edit, select the sequence to be edited and press Delete.

3. Press New and use the alphanumeric keypad to enter the

process indicated in the working procedure.

Enter the Formula, Concentration Units, Designation,

Measuring Ranges
For detailed information on entering the Formula, see section 6.1.2
on page 72.
Check the working procedure in advance to determine whether this
information needs to be changed.

1. Highlight the Formula line in the data overview and press Edit.

2. Select the formula, press Edit and use the alphanumeric

keypad to enter the data specified in the working procedure (for
C1=, C2=, units, name, measuring range limits, etc.).

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Enter the Variables (Factors, Wavelength and Conversion

Factors)
For detailed information on entering the Variables, see section 6.1.2
on page 72.
Check the working procedure in advance to determine whether this
point needs to be changed.

1. Highlight the Variables line in the data overview and press

Edit.

2. Select the variable, press Edit and use the alphanumeric

keypad to enter the data specified in the working procedure (for
F1, F2, λ1, U1 etc.). Confirm each entry with OK.

Abbreviation of Variables:
F1: Factor 1
F2: Factor 2
λ1: Wavelength 1
U1: Conversion Factor 1 for the first chemical form
U2: Conversion Factor 2 for additional chemical forms etc.

Timer 1, Timer 2, Timer 3 and Timer 4:

The timers are activated or deactivated with the control boxes on
the left of the display. In the next column, a selection can be made
from a list of names that describe the corresponding work step. In
the third column, the times for each active timer are entered.

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5.5.6.2 Update an existing barcode test

Note: Only in very few cases does the revision of a test require all test
specifications to be updated.

Option 1: Manual update of test data

1. The instrument needs to be at the Main Menu.

2. Insert the blank or sample cuvette/vial (depending on the

working procedure) in cell compartment #1 and close the cell
compartment.

3. After "Barcode controlnumber?", a data overview is displayed,

including the specifications of the test that is to be revised.

Note: The working procedures of the corresponding test contains the

new data (Wavelength, Factors, Measurement Ranges, Conversion
Factor for chemical Form, etc.).

4. Highlight the corresponding line and press Edit.

5. When finished programming, press Store and then Cancel to

return to the User Programs menu.

6. Press Start to complete the test with the new data.

Option 2: Manual check/revision of test data

If an incorrect entry was made during the data update and is not
recognized via the barcode of the cuvette/sample vial (e.g. an
incorrect measuring range), another option is available to check
and if necessary to update, the test data.

1. The instrument need to be at the Main Menu.

2. Insert the blank or sample cuvette/vial (depending on the

working procedure) into the cell compartment #1 and close the
cell compartment.

3. Press Options>More>Edit.

4. A data overview is displayed, including the specifications of the

test that is to be revised. Compare the displayed data with the
data in the working procedure and edit the displayed data in
accordance with the working procedure.

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5.5.6.3 Program a new test

1. The instrument needs to be at the Main Menu.

2. Insert the sample cuvette/vial or blank (depending on the

working procedure) into the cell compartment #1 and close the
cell compartment.

3. After "Program not available", a data overview is displayed,

including the specifications of the test that is to be revised.

The working procedures of the corresponding test contains the

new data (Wavelength, Factors, Measurement Ranges,
Conversion Factor for chemical Form etc.).

4. Highlight the corresponding line including the specifications of

the test that is to be programmed and press Edit.

5. To finish programming, press Store and then Cancel to return

to the Main Menu.

6. Press Start to run the test with the new data.

5.5.7 Upgrade of the instrument software

To obtain the software for the update from the Internet at
www.hach.com:

1. Go to http://www.hach.com.

2. On the DR 5000 product page, click Lab System

Software/Software Update Downloads under the heading
Downloads.

3. Locate the appropriate download and follow the prompts for

saving the file(s) to the USB memory stick.

4. On the DR 5000, press Instrument Update in the System

Checks menu.

5. Connect the USB stick to the USB interface on the DR 5000

(section 3.3 on page 14). Press OK.The link is established
automatically and the software is updated.

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6. Press OK to return to the System Checks menu.

Note: When the instrument software has been updated, a prompt to restart
the instrument is displayed.

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6.1 User Programs

User programs provide the opportunity to complete "made to
measure" analysis.
The User Programs database is empty when the instrument leaves
the factory and is used to accommodate programs created by users
specific needs. Here are a few examples of entries:
• Programming of user-created procedures. The analysis
procedure must be developed first, before it can be
programmed. The user must define or determine the program
sequences, calculation formulas, measurement wavelengths,
factors, measuring range limits, etc.
• Modified tests
• Assignment of user programs to the favorites menu for
frequently used tests.
• Creation of a specific selection of methods and tests.
Press User Programs in the Main Menu and then
Program Options. The Program Options menu contains several
input and editing options (Table 7):

Table 7 Program options user programs

Options Description
1 New Select New to program a new user program.
Note: The first time Program Options is selected, only the New option is available. The other
options remain inactive (gray) until the first program has been created.
1 Add to favorites Select Add to favorites to add an existing user program to the list of frequently used programs.
1 Edit Select Edit to modify an existing program
1 Delete Select Delete to remove a program from the list of user programs. The program will be
simultaneously deleted from the Favorites list.

6.1.1 Program a user method

All input steps and their significance and options are explained in
the following sections.

1. Select New in the Program Options menu.

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Program Number:
Specific test number, with which the program can subsequently be
called up from the selection list in the User Programs menu or the
Favorites menu.

2. Use the alphanumeric keypad to enter a program number

between 950 and 999. The lowest available number appears
automatically.

3. Press OK.

Note: If the program number is already assigned to another user

program, a message appears, asking whether the existing program
should be replaced. Press OK to overwrite the existing program.

Program Name:

4. Use the alphanumeric keypad to enter a program name. The

name can be a maximum of 28 characters long.

5. Press Back to go back to the previous program point or press

Next to continue with the input of the program data.

Program Type:

6. Select the required option (Table 8) and press Next.

7. If the Single Wavelength (section 6.1.1.1 on page 63) or Multi

Wavelength (section 6.1.1.2 on page 64) is selected, define the
unit, wavelength, absorbance formula, wavelength λx,
concentration factor Kx, concentration resolution, chemical
form and calibration equation.
More information on Free Programming parameters see
section 6.1.2 on page 72.

Table 8 Program descriptions

Program Type Description

Single Wavelength Measurements at a defined wavelength

In the Multi Wavelength mode, absorbance values can be measured at up to four wavelengths
Multi Wavelength
and the results can be mathematically processed to obtain sums, differences and relationships.
This is an advanced form of programming for original user-developed methods. In Free
Free Programming Programming, the user defines the measurement process, variables and calculations involved in
obtaining a reading.

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6.1.1.1 Single wavelength settings

If the Single Wavelength mode is selected, the following
parameters can be defined:

Units:
Select the required unit from the list and press Next.
Note: Units of measure not included in this list can be added in the edit
program under Program Options, Edit. Select Units, Edit and then New.

Wavelength (single wavelength program type):

Use the alphanumeric keypad to enter the measurement
wavelength. The entered wavelength must be in the range from
190–1100 nm.
Press Next to proceed.

Concentration resolution (number of decimal places)

Select the required number of decimal places from the displayed
list and press Next.

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Chemical form:
Enter the chemical formula used in the display to represent the
analysis parameter.
Use the alphanumeric keypad to enter the chemical form and press
Next to enter Calibration settings.

6.1.1.2 Multi wavelength settings

If the Multi Wavelength mode is selected, the following parameters
can be defined:

Units:
Select the required unit from the list and press Next.
Note: Units of measure not included in this list can be added in the edit
program under Program Options, Edit. Select Units, Edit and then New.

Absorbance formula (multi-wavelength program type):

The Absorbance Formula menu is used to define the wavelengths
and the coefficients used in the formula. The absorbance formula
defines the calculation for the multi-wavelength measurement.
Press the appropriate key to edit the input.
Press the Formula key.
In the displayed list, select the formula for the program and press
OK to confirm.

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List of available absorbance formulas

A1 is the absorbance at wavelength 1,
A2 is the absorbance at wavelength 2 and so on
K1 is the factor at wavelength 1,
K2 is the factor at wavelength 2 and so on
If a subtraction has to be completed, the factors can be entered
with a minus sign.

Wavelength λx:
Press a λx key and use the alphanumeric keypad to enter a
wavelength. Press another λx key and enter the next wavelength. If
necessary, repeat until all the wavelengths for the formula have
been entered. The wavelengths must be in the range from
190–1100 nm. Press OK.

Concentration Factor Kx
Multiplication factor for converting absorbance values into
concentration values.
Press a factor key and use the alphanumeric keypad to enter a
factor. If the formula includes more than one factor, press another
factor key and enter another factor and repeat until all the factors
have been entered. Press OK. When all the relevant data have
been entered, press Next.
Note: Up to five digits can be entered, including a maximum of 4 decimal
places to the right of the decimal.

Concentration resolution (number of decimal places)

Select the required number of decimal places from the displayed
list and press Next.

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Chemical form:
Enter the chemical formula used in the display to represent the
analysis parameter.
Use the alphanumeric keypad to enter the chemical form and press
Next to enter Calibration settings.

6.1.1.3 Calibration settings for single and multi wavelength mode

A method is calibrated by determining the absorbance values of
several standard solutions of known concentration.
There are three ways to create and store a calibration curve.
Instructions for each method follows (Table 9).

Table 9 Calibration settings

Mode Descriptions
A calibration table is created by entering the concentration values and the absorbance values of
Enter values the analyte solution. The absorbance values are plotted versus standard concentrations and the
calibration curve is displayed as a graph (page 66).
A calibration table is created by entering the concentration values of the standard solutions and
Read Standards then measuring the absorbance of the analyte solutions. The absorbance values are plotted
versus standard concentrations and the calibration curve is displayed as a graph (page 68).
If the calibration curve can be determined from the mathematical relationship between
concentration and absorbance by linear regression, etc., the corresponding formula can be
Enter Formula
selected (linear, 2nd or 3rd order polynomial) from a list and the appropriate factors can be
entered (page 69).

Calibration by entering calibration values

Enter concentration/absorbance

1. Select the Enter Values and press Next.

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2. To enter the standard concentrations and corresponding

absorbance values in the displayed table, press the "+" symbol.
Use the alphanumeric keypad to enter the values.

Press OK and enter the corresponding absorbance value.

Press OK.

The entered data are displayed in the table. Repeat the

sequence for each data point to enter it.

3. To change a value in the table, highlight the appropriate line,

press the unit key (e.g. mg/L) or Abs and enter the changed
value via the alphanumeric keypad.

4. When the data have all been entered, press Graph.

Note: The correlation coefficient (r2) is shown on the left below the
axes.

5. The linear equation corresponds to the standard setting. Press

Next Curve to display the polynomial 2nd order curve. Press
Next Curve again, to display the polynomial 3rd order curve.

6. Press Force 0 to change the setting from Off to On. The curve
now passes through the origin of the coordinate system.

Note: This may have an adverse effect on the correlation coefficient

(r2).

7. Press Table to display the table again.

8. When the table has been completed and the curve type has
been chosen, press Done when the graph is displayed or Exit
when the table is displayed. Go to section 6.1.1.4 on page 70.

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Calibration by reading standards

1. Press Read Standards and press Next.

2. To enter the standard concentrations in the displayed table,

press the "+" symbol. Use the alphanumeric keypad to enter
the standard concentration. Press OK.

3. Press the "+" symbol again (see arrow) and enter the next
standard concentration. Repeat this sequence until all standard
conentrations (maximum of 24 solutions) have been entered.

4. Highlight the line with the appropriate concentration and insert

the cuvette/cell with the corresponding standard solution.

5. Insert the zero solution into the cell compartment. Press Zero.

6. Insert the first standard solution into the cell compartment.

Press Read.

Insert the second standard solution into the cell compartment.

Press Read.

Repeat this sequence until all the standard solutions have been
measured (maximum of 24 solutions).

The entered and measured data are displayed in the table.

Note: To delete a standard concentration, highlight the appropriate line and
press the Delete icon.

The timer icon shown in the display helps to ensure, when

necessary, that the steps of an analysis are correctly timed (e.g.
reaction times, wait times, etc., can be exactly specified). When the
specified time has elapsed, an acoustic signal is emitted. The use
of the timer has no influence on the measurement program.

7. When the data have all been entered and the measurements
have all been completed, press Graph.

8. The linear curve corresponds to the standard setting. Press

Next Curve to display the polynomial 2nd order curve.Press
Next Curve again to display the polynomial 3rd order curve.

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9. Press Force 0 to change the setting from Off to On. The curve
then passes through the origin of the coordinate system.

Note: This may have an adverse effect on the correlation coefficient

(r2).

10. Press Table to display the table again.

11. When the table has been completed and the curve type has
been chosen, press Done when the graph is displayed or Exit
when the table is displayed. Go to section 6.1.1.4 on page 70.

Calibration by entering the formula

1. Press Enter Formula and press Next.

2. Press the formula key.

A list of available formulas (linear and 2nd and 3rd order

polynomial) is displayed. Up to 4 coefficients can be entered,
depending on the selected formula. Press the required formula.

3. Depending on the selected formula, the required coefficients

(a, b, c...) are displayed. Press the coefficient keys and enter
the corresponding values via the alphanumeric keypad. After
each entry, press OK to confirm.

Note: The coefficients can have 5 digits and can have a positive or a
negative sign.

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6.1.1.4 Store a user program

The input of the basic data is complete. An overview of the variable

program data is displayed.

4. To enter more specifications or change existing ones, highlight

the appropriate line and press Edit.

5. Select Store to save the user program.

6. Press the PC & Printer icon to send the program data to a

printer or to a USB memory stick (connect the USB memory
stick to the USB interface first).

Note: The test data will be formatted in the preinstalled PrgData folder
as a .csv and as a .lst file.

7. To transfer the program data from one instrument to another

create a new folder on the USB memory stick named dbhl and
copy the .lst files into this folder. (see section 6.8.2 on
page 106).

6.1.1.5 Additional user-defined parameters and functions

In addition to the previously defined basic data, additional
parameters and functions can be defined for user programs:
• measuring range upper and lower limits
• timer functions
• chemical forms

Upper and lower limits of the measuring range

It is possible to enter a maximum (upper) and minimum (lower)
measurement value. An error message is displayed if a reading is
above the upper limit or below the lower limit.

1. Highlight the appropriate line in the overview of the program

data and press Edit.

2. Set On and press the 0.000 key to enter the measuring range
limit. Confirm the entry by pressing OK.

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Timer 1 / Timer 2 / Timer 3 / Timer 4:

This function can define time intervals for up to four timers. Timer
designations such as Shake, Wait and Swirl can be assigned.

1. Highlight the appropriate line in the overview of the program

data and press Edit.

2. The timers are activated or deactivated with the check boxes in

the left part of the display. In the next column, a selection can
be made from a list of names that designate the corresponding
work step. In the third column, the times for each timer are
entered (in mm:ss).

Chemical form 2 / chemical form 3 / chemical form 4:

If a Chemical Form 1 has been defined, up to three additional
alternative forms can be entered here.

1. Highlight the appropriate line in the overview of the program

data and press Edit.

2. The chemical forms are activated or deactivated with the check

boxes in the left part of the display.

3. Press the left key to enter another chemical form with the
alphanumeric keypad and press OK to confirm. Press the right
key to enter the conversion factor to calculate the concentration
of the additional chemical form from the concentration of
Chemical Form 1 and press OK to confirm.

4. Press Store to save the program data. Press Cancel to return

to the Main Menu.

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6.1.2 Free programming program type

Free Programming is an advanced option for entering original
user-developed methods. When the Free Programming option is
selected, an overview of the specifications of the programmed test
is displayed. Each input option can be modified to develop the user
method. Refer to Table 10 for more information. To modify an input
option, select the appropriate line and press Edit.
Important Note: Perform steps 1–6 of Programming a New User
Method (section 6.1.1 on page 61) before proceeding with Free
Programming.

Highlight the line containing the program point that is to be edited or

defined and press Edit.

Table 10 Definitions of the program points

Program point Description

Name Name of the analysis parameter

Version An abbreviation or version number assigned by the user is entered here.

Exact definition of the test: the number of wavelengths at which measurements are made, the
Measurement Process number of absorbance measurements needed, the keys to be used, any waiting periods between
measurements, etc.
Formula Definition of the formulas with which the test result is calculated.
The number of variables shown in the display depends on the definition of the measurement
Variables process and the formulas. Input of the numerical values of the wavelengths, factors, constants,
etc.
Used to enter abbreviations and defined times for up to four timers. Highlight the appropriate line
Timer 1, Timer 2, and press Edit. The timers are activated or deactivated with the control boxes on the left of the
Timer 3, Timer 4 display. In the next column, a selection can be made from a list of names that describe the
corresponding work step. In the third column, the times for each active timer are entered.

6.1.2.1 Measurement process

The measurement process defines the handling and the
measurements of the test:
• At which and how many wavelengths should measurements be
completed
• How many absorbance measurements must be completed?
• When should the zero measurement and the sample
measurement be completed?
• Are waiting times necessary between measurements?

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• Should individual program sequences be repeated?

The elements of a measuring sequence, such as zero and sample
measurements and the timer(s) (reaction times, waiting times, etc.)
are individually defined.

6.1.2.2 Enter a new element of a measuring sequence

Important Note: Each component of the measurement process
must be entered in the order in which it will be completed.

1. Highlight the Measurement Process line in the data overview

and press Edit.

2. Press Edit again and then New.

Content and definition of the keys

[Z] key / Zeroing

1. Press the [Z] key to program a zero measurement. Confirm

with OK.

2. Press New and then Zeroing... and use the alphanumeric

keypad to enter the wavelength at which the zero measurement
is to be completed. Press OK and confirm the input by pressing
OK again.

3. If zero measurements are to be carried out at a number of

wavelengths, repeat the above two steps for each wavelength.

Note: The entered measurement sequence is displayed.

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Process Timer key

1. Press the Process Timer key to enter any waiting, reaction or

handling times that have to be taken into account. Use the
alphanumeric keypad to enter the time. Press OK and confirm
the input by pressing OK again.

Note: This time is integrated into the measurement process.

Note: The entered measurement sequence is displayed.

[R] key / Reading...

1. Press the [R] key to program a measurement of the substance

that is to be analyzed. Confirm with OK.

2. Press New and then Reading... and use the alphanumeric

keypad to enter the wavelength at which the measurement is to
be completed. Press OK and confirm the input by pressing OK
again.

3. If measurements are to be completed at a number of

wavelengths, repeat the above two steps for each wavelength.

Note: The entered measurement sequence is displayed.

{ } key
Elements of the measurement sequence that are to be repeated
are placed in brackets.
The left bracket "{"marks the start of the sequence that is to be
repeated and the right bracket "}" marks the end.
Note: The key showing the right bracket remains inactive until a left bracket
is entered.

1. Press {.

2. Press the key that defines the sequence that is to be repeated:

[Z] or [R]. Confirm with OK.

3. Press New and then press Zeroing... or Reading... and use

the alphanumeric keypad to enter the wavelength at which the
measurement should be completed. Press OK and confirm the
input by pressing OK again.

4. Press } to end the sequence.

Note: If an action such as a zero measurement recurs at different

stages of a measurement sequence, the series of actions is numbered
sequentially (e.g. Z1, Z2, etc.).

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Delete an element of a measuring sequence

Select the appropriate line and press Delete. The element is
deleted.

Insert an element of a measuring sequence

Select the line in the measuring sequence where the insertion is be
made and press New.
A new element can be entered at the selected position.
When the input is complete, press OK in the "Measurement
Process" display. The data overview is then displayed.

6.1.2.3 Enter the calibration formula (evaluation formula)

The calibration formula (evaluation formula) defines the calculation
and display of intermediate and final results. The previously defined
elements of the measuring sequence are the basis for calculating
the concentrations.

Enter calibration formula C1

1. Highlight the Formula line in the data overview and press Edit.

2. Highlight the line C1: Off and press Edit.

3. Select C1: Off again and press Edit. The display changes to
C1: On.

4. Highlight the next line C1 = to define the formula and press

Edit.

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Refer to Table 11 for detailed information on the Edit

formula keys.
Note: The evaluation formula is built up successively in the display in
accordance with the input.

Note: The arrow key deletes the most recently entered element of the
formula.

Table 11 Edit formula key descriptions

Screen Key Description

Press the Abs/Variables key to select, from the

displayed list, the required element of the defined
Abs/Variables measuring sequence and therefore the corresponding
measurement wavelength, so that this can be taken into
account in the formula.

New Number Press New Number to enter a new factor or constant.

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Table 11 Edit formula key descriptions (continued)

Screen Key Description
Press +– ÷x, to enter a mathematical operation.
Select the operation and press OK to confirm.
The available choice of mathematical operations
depends on the defined formula.
This means that functions such as "( )" or "ln"/"log" etc.
are only active if a term in parentheses or the calculation
of a logarithm is mathematically permissible in the
defined formula (this also applies to the basic
mathematical operations).
The following mathematical operations are available:
+– ÷x
• + (Addition)
• – (Subtraction)
• ÷ (Division)
• x (Multiplication)
• ^ (Exponent)
• Ln (Natural logarithm)
• Log (Common logarithm)

Press >=< to include logic statements/links/conditions in

the formula. The following functions are available:
= (Equal to), < (Less than), > (Greater than), <= (Less
than or equal to), >= (Greater than or equal to), IF,
THEN, ELSE
>=< When the evaluation formula C1 has been entered
completely, press OK to confirm. Press OK again to
return to the Formula display.
When the formula C1 has been entered and confirmed,
the parameter name, upper and lower limits of the
measuring range and display result (yes, no) can be
entered.

Enter the next calibration formula (C2 or Cn)

5. Highlight C2: Off and press Edit.

6. Select C2: Off again and press Edit. The display switches to
C2: On.

7. Highlight the next line C2 = to define the formula and press

Edit.

In addition to the keys described in Table 11, only one other

function is available:

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Select Conc. key

If an already defined formula, in this case C1, is to be taken into
account in the formula for C2, press Select conc.
Enter the number of the formula (e.g. 1 for C1) and press OK to
confirm.
Cn can now be linked with a mathematical operation.
Note: The Cn concentrations that are to be calculated are numbered in
sequence: C1, C2, C3, etc.

Note: When the first Cn formula has been defined, the Formula list is
automatically extended by Cn+1.

6.1.2.4 Enter variables

1. Highlight the Variables line in the data overview and press

Edit.

2. Select the variable to be edited, press Edit and use the

alphanumeric keypad to enter the data specified in the working
procedure (for F1, F2, l1, U1 etc.). Press OK to confirm each
entry.

Abbreviation of Variables:
F1: Factor 1
F2: Factor 2
λ1: Wavelength 1
U1: Conversion Factor 1 for the first chemical form
U2: Conversion Factor 2 for the further chemical form etc.

6.1.2.5 Save a free programming user program

1. Press Store to save the entered data. The data can be stored
under any data point (Measurement sequence, Formula, Timer,
etc.).

2. Press the PC & Printer icon to send the program data to a

printer or to a USB memory stick (connect the USB memory
stick to the USB interface first).

Note: The test data will be formatted in the preinstalled PrgData folder
as a .csv and as a .lst file.

3. To transfer the program data from one instrument to another

create a new folder on the USB memory stick named dbhl and
copy the .lst files into this folder (see section 6.8.2 on
page 106).

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6.1.3 Select a user program

1. Press User Programs in the Main Menu to view an

alphabetical list of user programs with program numbers.

The User Programs list will appear.

Note: Use the scroll bar to scroll through the list quickly.

Note: Highlight the selection by pressing it or press Select by

Number to search for the program by number.

2. Press Start to run the program.

6.1.4 Add, edit and delete user programs from the favorites list
The most frequently used tests/methods in the User Program menu
can also be added to the list of favorites to simplify their selection.

1. Press User Programs in the Main Menu.

The User Programs list will appear.

2. Highlight the selection by pressing it or press Select by

Number to search for the program by number.

Note: Use the scroll bar to scroll through the list quickly. Use the
alphanumeric keypad to enter the test number (program number) and
press OK to confirm.

3. Press Program Options.

4. Press Add to Favorites, Edit or Delete and press OK to

confirm.

Note: If the stored program is deleted in User Programs, it will also be

deleted in Favorites Programs.

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6.1.4.1 Add to Favorites

1. Press Add to Favorites and press OK to confirm.

The program is added to the Favorites.

6.1.4.2 Edit

1. Press Edit and press OK to confirm.

An overview of the specifications of the programmed test is

displayed. More information about the input options is provided
in the section 6.1.2 on page 72.

6.1.4.3 Delete

1. Press Delete and press OK to confirm.

The program is deleted from the list of User Programs.

Note: If the stored program is deleted in User Programs, it will also be

deleted in Favorites Programs.

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6.2 Favorite Programs

The most frequently used tests/methods in the Stored Programs
menu and the User Programs menu can also be added to the list
of favorites to simplify their selection.
To add Stored Programs and/or User Programs to the favorites
list or the favorite programs, see section 6.1.4 on page 79.

6.2.1 Recall a favorite program

1. Press Favorite Programs in the Main Menu.

The Favorite Programs list will appear.

2. Highlight the selection by pressing it.

Note: Use the scroll bar to scroll through the list quickly.

Note: Press Select by Number to search for the program by number. Use
the alphanumeric keypad to enter the test number (program number) and
confirm the input by pressing OK.

3. Press Start.

6.2.2 Delete a favorite program

1. Press Favorite Programs in the Main Menu.

The Favorite Programs list will appear.

2. Highlight the selection by pressing it.

Note: Use the scroll bar to scroll through the list quickly.

Note: Press Select by Number to search for the program by number. Use
the alphanumeric keypad to enter the test number (program number) and
confirm the input by pressing OK.

3. Press Remove Program and press OK to confirm.

Note: If a Favorite Programs is deleted, it will stay in the User

Programs or Stored Programs.

Note: If the stored program is deleted in User Programs, it will also be

deleted in Favorites Programs.

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6.3 Standard Addition – monitoring/checking results

The accuracy of measured values (their correspondence with the
actual concentration of the analyte in the sample) and their
precision (correspondence of the measurement results obtained
from several samples containing the same concentration of the test
analyte) can be determined or improved using the standard addition
method.
This method (also referred to as spiking) serves to identify
sample-specific interference factors, e.g. substances in the sample
that falsify the analysis (sample matrix effect), a defective
measuring instrument or contaminated reagents.

Method:
A defined amount (concentration) of a standard solution of the test
substance is added to the sample. The detection rate should be
close to 100%.

Measured value after a standard addition

Detection rate = -------------------------------------------------------------------------------------------------------------
Expected value after a standard addition

Detection rate Conclusion

Probability that the measurement results are correct
100%
is high.
Assumption: The analysis was falsified by
substances in the sample (sample matrix effect)
Test to determine whether a sample matrix effect
< 100%
is present:
Use distilled water instead of the sample. Add
standard solution as described in the procedure.
Detection rate Conclusion
Ions in the sample are
interfering with the
100%
analysis, causing false
results to be obtained.
No interfering ions -
≠ 100% consider other
interference factors.

Measures to identify other interference factors:

Checklist:

1. Check if the procedure is completed correctly:

a. Are the reagents added in the correct order?

b. Is enough time allowed for color development?

c. Is the correct glassware in use?

d. Is the glassware clean?

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e. Does the test require the sample to be at a certain

temperature?

f. Was the pH of the sample in the correct range?

g. Is the pipette volume correct?

2. Check the used reagents by repeating the standard addition

procedure with freshly prepared reagents.

Detection rate Conclusion

The originally used reagents were defective.
Check the standard solution:
100%
Repeat the standard addition procedure with a
freshly prepared standard solution.
Detection rate Conclusion
The originally used
100% standard solution was
defective.

If none of these measures resolves the problem, please contact the

manufacturer or a sales representative.

6.3.1 Complete a standard addition

Carry out the standard addition in accordance with the
corresponding procedure.
There are two different methods:

Peak volume (Standard addition):

Defined volumes of a standard solution are added step by step to
an already analyzed sample. The sample is measured again after
each addition.

Sample volume:
A defined volume of a standard solution of known concentration is
added to the volume of sample specified in the procedure and the
sample is measured after each addition. In most cases three
different standard solutions are prepared and the procedure is
repeated for each of them.
Note: The units and chemical forms used for the sample are used for the
standard solutions. Be careful to ensure that to use the correct units for
subsequent entries.

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Peak volume/sample volume methods

1. Select Stored Programs in the main menu. Select the required

program.

2. Press Start.

3. Analyze a sample without added standard solution in

accordance with the instructions in the Procedures Manual.
When the measurement is complete, leave the sample
cuvette/cell in the cuvette/sample cell holder.

4. Press Options>More>Standard Addition.

An overview of the data of the standard addition procedure is

displayed.

5. Press OK to accept the standard values for standard

concentration, sample volume (total) and standard addition
volume. Press Edit to change any of these values.

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6. Press the key of the value to change it. Use the alphanumeric
keypad to change the value. Press OK to confirm.

7. Press the keys to enter the standard addition volumes. Use the
alphanumeric keypad to enter the new data and press OK.

Description of the table of readings

• The first column shows the standard addition volume. 0 mL
stands for a sample containing no added standard.
• The second column shows the reading of the samples with and
without added standard.
• The third column shows the detection rate of the added
standard.
Note: The black highlighted line is active.

8. The reading of the sample in the instrument, without added

standard, is automatically shown under 0 mL.

9. Prepare the standard addition solution as described in the

procedure.

10. Use the arrow keys to select the first standard addition volume
in the table and insert the cuvette/cell with the corresponding
volume of added standard into the cell compartment.
Press Read.

Repeat the procedure from point 8 with all the other standard
addition solutions.

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11. After all the standard addition solutions have been measured,
press Graph.

The regression line through the standard addition data points is

displayed.

The correlation coefficient r2 indicates how close the data

points are to the line.

If the correlation coefficient = 1, the curve is linear.

The concentration shown above the curve is the estimated

concentration of the sample without the added standard.

Note: In the curve menu, the name on the Curve key switches to
Table. Press Table to display all the data in the table again.

12. Press Ideal line to display the relationship between the added
standard solutions and the ideal line (detection rate 100%).

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6.4 Single Wavelength (absorbance, concentration and transmittance

measurements)
The Single Wavelength mode can be used in three ways. For
sample measurements at a single wavelength, the instrument can
be programmed to measure the absorbance, % transmittance or
concentration of the analyte.
Absorbance measures the amount of light absorbed by the sample,
in units of absorbance.
% transmittance measures the percent of the original light that
passes through the sample and reaches the detector.
Turn the concentration factor on to select a specific multiplier for
converting absorbance readings to concentration. In a graph of
concentration versus the absorbance, the concentration factor is
the slope of the line.

6.4.1 Set up single wavelength mode

Press Single Wavelength in the Main Menu. Press Options for
Parameter Setup.

Table 12 Single wavelength setup options

Options Description

More For further Options

With the Store On setting, all measurement data are stored automatically. With the Store Off
Store Off/On
setting, no measurement data are stored.
% Trans/Abs To switch to % transmittance, concentration or absorbance readings
To enter the measurement wavelength. Use the alphanumeric keypad to enter the measurement
λ Wavelength
wavelength. The entered wavelength must be in the range from 190–1100 nm.
This functions as a stopwatch. It helps to ensure that the steps of an analysis are correctly timed
(e.g. reaction times, wait times, etc., can be exactly specified). When the specified time has
Timer icon
elapsed, an acoustic signal is emitted. The use of the timer has no influence on the measurement
program.
Concentration Factor Multiplication factor for converting absorbance values into concentration values.

Concentration
To select the position of the decimal point in the calculated concentration readings.
Resolution

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Table 12 Single wavelength setup options (continued)

Options Description
Single Reading Mode: A reading is only displayed after a measurement has been carried out
(press Read; standard setting) (see section 6.4.2 on page 89).
Reading Mode
Continuous Reading Mode: After the zero measurement, all readings are displayed
automatically and continuously (see section 6.4.3 on page 89).
Save as User Program To store the selected parameters as a User Program, see section 6.1 on page 61
Call up saved measurement data, wavelength scans or time courses, see section 5.3 on
Recall Data
page 35.
Instrument Setup Basic data of the instrument, see section 5.2 on page 23.

Concentration factor:

1. Press Concentration Factor: Off in the Options menu. Press

On to highlight this feature.

2. Press the ”Factor“ key and use the alphanumeric keypad to

enter the factor by which absorbance readings are to be
multiplied. Press the ”Unit“ key to select the units for
concentration measurements or to create a new unit.

3. Press OK to confirm.

Concentration resolution:

1. Press Concentration Resolution in the Options menu.

2. Select the resolution and press OK to confirm.

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Reading mode:

1. To highlight the required mode, start by pressing Reading

mode.

2. Select the required mode, then press OK, then Return to return
to the result display.

6.4.2 Take single wavelength measurements (single reading)

1. Insert the blank cuvette/cell into the cuvette/sample cell holder.

Press Zero.

Note: The Read key is only active after the zero measurement has
been completed.

2. Insert the sample cuvette/cell into the cuvette/sample cell

holder. Press Read.

3. For data storage, see section 5.3.2 on page 38.

6.4.3 Take single wavelength measurements (continuous readings)

1. Insert the blank cuvette/cell into the cuvette/sample cell holder.

Press Zero.

Note: In the reading mode ”Continuous“ only the Zero key is shown to
start the reading. The reading sequence is started automatically.

2. Insert the sample cuvette/cell into the cuvette/sample cell

holder.

3. Press Options and then the Store icon to store the displayed
data in the Data Log.

Note: For data storage, see section 5.3.2 on page 38.

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6.5 Multi-Wavelength mode – measurements at more than one wavelength

In the multi-wavelength mode, absorbance values can be
measured at up to four wavelengths and the results can be
mathematically processed to obtain sums, differences and
relationships.
Absorbance measures the amount of light absorbed by the sample,
in units of absorbance.
% Transmittance measures the percent of the original light that
passes through the sample and reaches the detector.
Turning on the concentration factor allows selection of a specific
multiplier for converting absorbance readings to concentration. In a
graph of concentration versus the absorbance, the concentration
factor is the slope of the line. Concentration is calculated using a
single factor for each wavelength, which is input by the user.

6.5.1 Set the reading mode at different wavelengths

Press Multi Wavelength in the Main Menu. Press Options for
Parameter Setup.

Table 13 Multi-wavelength setup options

Options Description

More For further Options

With the Store On setting, all measurement data are stored automatically. With the Store Off
Store Off/On
setting, no measurement data are stored.
% Trans/Abs To switch to % transmittance, concentration or absorbance readings
To enter the measurement wavelength. Use the alphanumeric keypad to enter the measurement
λ Wavelength
wavelength. The entered wavelength must be in the range from 190–1100 nm.
This functions as a stopwatch. It helps to ensure that the steps of an analysis are correctly timed
(e.g. reaction times, wait times, etc., can be exactly specified). When the specified time has
Timer icon
elapsed, an acoustic signal is emitted. The use of the timer has no influence on the measurement
program.
Concentration Factor Multiplication factor for converting absorbance values into concentration values.

Concentration
To select the position of the decimal point in the calculated concentration readings.
Resolution

Absorbance Formula Calculation basis for evaluating samples

Save as User Program To store the selected parameters as a User Program, see section 6.1 on page 61

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Table 13 Multi-wavelength setup options (continued)

Options Description
Call up saved measurement data, wavelength scans or time courses, see section 5.3 on
Recall Data
page 35.
Instrument Setup Basic data of the instrument, see section 5.2 on page 23.

λ / Absorbance formula:

1. Press Absorbance Formula.

2. The formula selected in the top key determines the number of

wavelength and coefficent keys that will appear below. To
change the absorbance formula, press the top key, select a
formula from the displayed list and press OK. When a new
formula is selected, the number of variables below changes to
match.

The following formulas are available:

K1A1+K2A2
K1A1+K2A2+K3A3
K1A1+K2A2+K3A3+K4A4
K1A1/K2A2
(K 1 A 1 + K 2 A 2 ) / K 3 A 3
(K 1 A 1 + K 2 A 2 ) / (K 3 A 3 + K 4 A 4 )

A 1 refers to the absorbance at wavelength 1

A 2 refers to the absorbance at wavelength 2, etc.
K 1 refers to the coefficient at wavelength 1
K 2 refers to the coefficient at wavelength 2, etc.
Coefficients can be set negative where subtraction is required.

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3. To change a wavelength, press one of the ”λx:“ keys. Enter the

desired wavelength coefficient into the numeric keypad.
Press OK to confirm.

4. To change a coefficient, press one of the ”KX:“ keys. Enter the

desired coefficient into the numeric keypad.
Press OK to confirm.

Note: The instrument allows entry of up to 5 significant digits, with a

maximum of 4 significant digits after the decimal point.

Concentration factor:

1. Press Concentration Factor: Off in the Options menu. Press

On to highlight this feature.

2. Press the ”Factor“ key to enter the factor by which

absorbance readings are to be multiplied. Press the ”Unit“ key
to select the units for concentration measurements or to create
a new unit.

3. Press OK to confirm.

Concentration resolution:

1. Press Concentration Resolution in the Options menu.

2. Select the resolution and press OK to confirm.

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6.5.2 Complete a measurement in the multi wavelength mode

1. Insert the blank cuvette/cell into the cuvette/sample cell holder.

Press Zero.

Note: The Read key does not become active until the zero
measurement has been completed.

2. Insert the sample cuvette/cell into the cuvette/sample cell

holder. Press Read.

Note: The UV lamp is not switched on until a wavelength in the UV

spectrum has been entered and the blank reading has been started.
During the warm-up phase of the UV lamp, the message ”Warming
up...“ is displayed and the ”UV lamp“ symbol flashes. As soon as the
UV lamp is ready, the blank reading is carried out.

Note: Zero and Read are disabled until the cell compartment is
closed.

3. For data storage, see section 5.3.1.1 on page 35.

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6.6 Wavelength Scan mode – recording of absorbance and transmittance

spectrums
In the wavelength scan mode, the absorbance of the light in a
solution over a defined wavelength spectrum is measured.
The measurement results can be displayed as a curve, as percent
transmittance (%T) or as absorbance (Abs). The collected data can
be printed as a table or a curve.
The data are available for formatting changes. These include
automatic scaling and zoom functions. Maximum and minimum
values are determined and shown as a table.
The cursor can be moved to any point on the curve for the purpose
of reading off the absorbance or transmittance value and the
wavelength. The data associated with each data point can also be
shown as a table.

6.6.1 Set up the wavelength scan

Press Wavelength Scan in the Main Menu. Press Options for
Parameter Setup.

Table 14 Wavelength scan setup options

Option Description

More For further Options

Store icon To store the scan data

From the displayed list of stored scans, a record is selected for use as a reference
scan/superimposed scan. This can be highlighted or shown in the background in comparison
Reference Off/On with the actual measured scan.
Note: This option is only available when there are stored scans with the same wavelength range
and step.
λ To enter the wavelength spectrum and the scan interval
Enables the user to switch the display back and forth between the scan data tables
Select View (wavelength/absorbance) and the graph of the curve.
Note: Select View will be activated after the first reading.
To select Track or Peak/Valley. The selection for this menu item determines to which points on
Cursor Mode
the graph the cursor moves.
Send Data To send Data to a printer, computer or USB memory stick (Type A)

Integral: On/Off The integral gives the area and the derivative of the integral gives the original function

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Table 14 Wavelength scan setup options (continued)

Option Description
Scale: In the automatic scaling mode, the y-axis is automatically adjusted so that the total scan is
displayed.
Scale & Units
The manual scaling mode allows sections of the scan to be displayed.
Units: Choice of absorbance or transmittance.
Call up saved measurement data, wavelength scans or time courses, see section 5.3 on
Recall Data
page 35.
Instrument Setup Basic data of the instrument, see section 5.2 on page 23.

λ Setting wavelength

1. Press the λ key in the Options menu to select the wavelength

range and the wavelength step.

2. Press the upper left key to open the numeric keypad and select
the minimum wavelength. Press OK to confirm.

3. Press the upper right key to open the numeric keypad and
select the maximum wavelength. Press OK to confirm.

Note: Do not select the same wavelength for minimum and maximum.

4. Highlight the required wavelength step.

Note: Scan recordings of high resolution data take a longer time than
recordings of low resolution data.Selecting a larger step allows the
instrument to scan faster, but decreases the resolution of the collected
data.

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5. According to the selected wavelength range, select the interval

from the actively displayed wavelength steps. In summery
maximally 780 measuring steps can be accomplished during a
Scan.

Note: The maximum wavelength adjusts automatically if the difference

between the maximum and minimum wavelength is not a multiple of
the interval.

6. Press OK to return to the scan mode. Selected parameters are

displayed along the graph‘s x-axis.

Select view (displaying table)

1. Press Select View in the Options menu after a reading is

taken.

2. A table with the results is displayed.

3. To return to the graph press Options and then View Graph.

Cursor mode

1. Press Cursor Mode: Track in the Options menu.

2. The selection for this menu item determines what data are
displayed in the table. Highlight Track or Peak/Valley.

3. Press OK to confirm.

4. Press Return to return to the scan mode.

Integral
The Integral applies to the whole wavelength range of the scan.

1. Press Integral: Off in the Options menu.

2. Highlight On to show the Integral. To find the integral of other

wavelength ranges, change the wavelength range and scan
again.

3. Press OK to confirm.

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4. Press Return to return to the scan mode.

Note: The Integral is shown instead of the date on the display.

Note: For the next scan measurement the setting for the Integral will be
On.

Scale & units

1. Press Scale & Units.

2. Highlight the required units (Abs or %T).

3. Highlight Auto or Manual scaling on the graph´s y-axis.

Note: If manual scaling is selected, use the alphanumeric keypad to set the
limits ymin. and ymax.. The graph is adjusted to display only the values in the
selected range. If automatic scaling is selected, the instrument sets the
limits automatically so that the total range can be displayed.

4. Press OK.

5. Press Return to return to the scan mode.

6.6.2 Wavelength scan reading

After the scanning parameters have been selected, the baseline
must be scanned. Changing any of the scanning parameters
requires a new baseline scan. When the baseline has been
scanned, the instrument is ready to scan one or more samples.

1. Press Wavelength Scan in the Main Menu.

2. Insert the blank cuvette/cell into the cuvette/sample cell holder.

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3. Press Zero. ”Zeroing....“ appears below the graph as the

baseline scan begins.

4. Insert the prepared sample cuvette/cell into the cuvette/sample

cell holder.

5. Press Read. Below the graph ”Reading...“ appears and a graph

of the absorbance or transmittance values at the scanned
wavelengths is displayed continuously.

The Wavelength Scan is complete, if

• the graph is shown full size,
• the scaling of the x-axis fits automatically,
• the Cursor functions in the vertical navigation bar are
highlighted.

6.6.2.1 Navigation of the wavelength scan graph or a wavelength scan analysis

Table 15 Navigating the wavelength scan

Cursor Function/
Description
Zoom Function

Curve Icon Choice of Cursor Mode Peak/Valley (cursor moves between minimum/maximum absorbance
(Choice of Cursor Mode) values) or Cursor Mode Tracking (cursor moves over each data point of the scan).
The arrow keys are used (right/left) to move the cursor (depending on the selected mode) to the
next data point. The data of the data point (wavelength/absorbance or transmittance value) are
Arrow keys highlighted on the x and y axes.
Note: Press any point on the curve to display the associated data.
This function is used to magnify the section of the curve in the vicinity of the cursor. The original
Zoom Icon
curve size can be restored by pressing the zoom icon again.

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6.6.3 Work with reference scans

There are two options to work with Reference Scan:

Option 1:

1. Press Reference: Off in the Options menu to select another

scan to display on the same screen with the current scan.
Highlight the required scan number and press
Highlight Reference.

Note: After selecting a reference scan the Reference: Off key in the
Options menu turns into Reference: On.

Note: Only scans that have the same wavelength range and step can
be displayed using the overlay option. A reference curve can only be
displayed with another active curve. Two previously stored scans
cannot be overlaid.

2. The reference curve is shown in gray. The absorbance or

transmittance value and the associated wavelength are
highlighted in gray.

Note: A black and an gray box are shown in the left corner of the
display. The gray box relates to the reference scan and the black one
relates to the current wavelength scan.

3. To complete the wavelength scan reading, see section 6.6.2 on

page 97.

• The newly plotted wavelength scan curves are shown in

black.
• The absorbance or transmittance value and the associated
wavelength are highlighted in black.
• In addition, the display shows the difference between the
wavelength scan curve and the reference curve against the
wavelength.

4. Press the black or gray small box in the left upper corner on the
screen to switch between the actual wavelength scan and
reference scan.

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Option 2:

1. Insert the blank cuvette/cell into the cuvette/sample cell holder.

Press Zero.

2. Insert the sample cuvette/cell into the cuvette/sample cell

holder. Press Read.

• The newly plotted wavelength scan curves are shown in

black.
• The absorbance or transmittance value and the associated
wavelength are highlighted in black.

3. Press Options and then Reference: Off in the Options menu

to select another scan to display on the same screen with the
current scan. Highlight the required scan number and press
Highlight Reference.

Note: After selecting a reference scan the Reference: Off key in the
Options menu turns into Reference: On.

Note: Only scans that have the same wavelength range and step can
be displayed using the overlay option. A reference curve can only be
displayed with another active curve. Two previously stored scans
cannot be overlaid.

4. The reference curve is shown in gray. The absorbance or

transmittance value and the associated wavelength are
highlighted in gray.

• Additionally the difference of the extinction and/or

transmission value between the two Scans (measured
Scan and reference Scan) is indicated/highlighted at each
position of the cursor.
Note: A black and an gray box are shown in the left corner of the
display. The gray box relates to the reference scan and the black one
relates to the current wavelength scan.

5. Press the black or gray small box in the left upper corner on the
screen to switch between the actual wavelength scan and
reference scan.

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6.7 Time course of absorbance/transmittance

The Time Course Mode is used to collect data in either absorbance
or transmittance for a user-specified length of time. After the data
are collected, they can be displayed in either graphic or tabular
format.

6.7.1 Time course setup parameters

Press Time Course mode in the Main Menu. Press Options to
configure parameters.

Table 16 Time course setup options

Option Description

More For further Options

Store icon To store the scan data

To input the total time for data collection and the time interval between the collection of data
Time & Interval
points
λ To input the wavelength setting
To display readings in absorbance, transmittance or concentration. This can be changed after
View Table/Graph
sample data are collected
Scale: In the automatic scaling mode, the y-axis is automatically adjusted so that the total scan is
displayed.
Scale & Units
The manual scaling mode allows sections of the scan to be displayed.
Units: Choice of absorbance or transmittance.
Send Data To send Data to a printer, computer or USB memory stick (Type A)
Call up saved measurement data, wavelength scans or time courses, see section 5.3 on
Recall Data
page 35.
Instrument Setup Basic data of the instrument, see section 5.2 on page 23.

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Time & interval:

1. Press Time & Interval in the Options menu.

2. Input the total time and the reading time and press OK to
confirm.

Note: In total 500 measuring steps are possible. To select a total time and
a time interval that would cause this number of measurements to be
exceeded, the time interval is defined automatically and the OK key is
inactivated.

Scale & units:

1. Press Scale & Units in the Options menu.

2. Highlight Abs or %T as the required units.

3. Highlight Auto or Manual scaling on the graph´s y-axis.

Note: If manual scaling is selected, use the alphanumeric keypad to set the
limits ymin. and ymax.. The graph is adjusted to display only the values in the
selected range. If automatic scaling is selected, the instrument sets the
limits automatically so that the total range can be displayed.

4. Press OK to confirm.

5. Press Return to return to the scan mode.

6.7.2 Time course scan reading

After the parameters have been selected, the instrument must be

blanked, then the sample can be analyzed.

1. Insert the blank cuvette/cell into the cuvette/sample cell holder.

Press Zero. The blank reading is shown on the display.

2. Insert the sample cuvette/cell into the cuvette/sample cell

holder. Press Read. Start collecting time course (kinetic) data.

Note: During the measurement the Zero and Read keys change to
Mark and Stop.

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• Select Mark to mark the next data point collected. This mark is
not used by the instrument, but is available for the user and
may indicate a significant event, such as the addition of a
sample or other reagent. The mark is also shown in the table.
• Select Stop to stop taking sample readings.

6.7.3 Analysis of time course data

After the data are collected, the following manipulations can be
done on the graphic data:

The Time Course Program is complete, if

• the sound is turned on, the instrument beeps when the
readings are done
• the graph is shown fullsize,
• the scaling of the x-axis occurs automatically,
• the Cursor functions in the vertical navigation bar are
highlighted.

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6.7.3.1 Navigation of a time scan or a time scan analysis

After a time scan has been completed, the time and the
absorbance/transmittance data are displayed as a curve.
Where the cursor is positioned on the curve, the elapsed time up to
this point and the corresponding absorbance are highlighted.

Table 17 Navigating the time scan

Cursor Function/
Description
Zoom Function
Delta mode: A second cursor is highlighted. The position of the fixed cursor was previously
defined in Cursor Mode Single. Use the active cursor to select any point on the measurement
curve. The difference to the fixed cursor is shown on the curve. The delta values are
Curve Icon correspondingly highlighted and displayed on the x and y axes.
(Choice of Cursor Mode) The gradient of the curve and the correlation coefficient (r2) between the cursor points in the
Delta mode are shown under the curve.
Cursor Mode Single: The cursor moves to each selected measurement point of the scan.
The arrow keys (right/left) are used to move the cursor (depending on the selected mode) to the
next data point. The data of the data point (wavelength/absorbance or transmittance value) are
Arrow keys highlighted on the x and y axes.
Note: Press any point on the curve to display the associated data.
This function is used to magnify the section of the curve in the vicinity of the cursor. The original
Zoom Icon
curve size can be restored by pressing the zoom icon again.

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6.8 System checks

1. Press System Checks in the Main Menu.

The System Checks menu contains instrument information and

various performance tests.

6.8.1 Instrument information

1. Press Instrument Information in the System Checks menu.

2. The model, serial number and software version are displayed.

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6.8.2 Upgrade of the instrument software

To obtain the software for the update from the Internet at
www.hach.com:

1. Go to http://www.hach.com.

2. On the DR 5000 product page, click Lab System

Software/Software Update Downloads under the heading
Downloads.

3. Locate the appropriate download and follow the prompts for

saving the file(s) to the USB memory stick.

4. On the DR 5000, press Instrument Update in the System

Checks menu.

5. Connect the USB stick to the USB interface on the DR 5000

(section 3.3 on page 14). Press OK.The link is established
automatically and the software is updated.

6. Press OK to return to the System Checks menu.

Note: When the instrument software has been updated, a prompt to restart
the instrument is displayed.

6.8.3 Optical checks

For each optical check, the measured results must be evaluated
against user-specific requirements. The check options do not
define tolerances.

1. Press Optical Checks in the System Checks menu.

The Optical Checks menu contains programs for checking the

wavelength accuracy, stray light and photometric accuracy.
An optional test filter set (Verification Kit) (Section 9 on page 129)
containing six precision glass filters, target values, tolerances and
instructions is available and is recommended as an aid for carrying
out comprehensive in-house instrument checks.

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6.8.3.1 Wavelength check

The Wavelength Check test is used to check wavelength accuracy
at 656.1 and 486.0 nm and to check bandwidth at 656.1 nm, only.

1. Press Wavelength Check in the Optical Checks menu.

2. Press λ to select the wavelength.

3. Make a choice and confirm with OK.

4. Press Start.

Note: During the warm-up phase of the UV lamp, the message ”Lamp
Warmup...“ is displayed.

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5. The result is displayed.

6. Press Cancel to return to Optical Checks.

6.8.3.2 Noise check

The Noise Check test is used to test the photometric noise in the
instrument.
This test can be used to test noise at any wavelength and at an
absorbance level determined by a specific sample.

1. Press Noise Check in the Optical Checks menu.

2. Press λ to input the wavelength.

3. Input the wavelength and press OK to confirm.

4. Press Zero.

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5. Insert sample cuvette/cell into the cell compartment. Close the

cell compartment.

6. Press Start.

7. The result will be displayed. Thirty readings are averaged for

the blank. The ”Average“ and ”Std Deviation“ are calculated
from 100 consecutive absorbance readings (Noise, Sample,
Reference).

Note: Failings and passings will be defined by the user.

8. Press PC & Printer icon to send data to a PC or Printer.

9. Press Cancel to return to Optical Checks.

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6.8.3.3 Stray light check

The Stray Light test is used to measure the stray light in the
instrument at 220.0 nm and 340.0 nm.

1. Press Stray Light Check in the Optical Checks menu.

2. Press λ to select the wavelength.

3. Make a choice and confirm with OK.

4. Press Zero.

5. Insert the sample cuvette/cell or reference filter into the cell

compartment. Close the cell compartment. Press Start.

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6. The ”Average“ and ”Std Deviation“ are calculated from

100 successive absorbance measurements. The result is
displayed and is to be compared with the nominal/standard
data (given in the quality control certificate) of the sample
cell/test filter.

Note: Failings and passings will be defined by the user.

7. Press Cancel to return to Optical Checks.

6.8.3.4 Absorbance check

The Absorbance Check test is used to test the photometric

accuracy and repeatability of the instrument.
This test can be used to test absorbance at any wavelength by a
specific sample or test filter set (Refer to section 6.8.3.6 on
page 113).

1. Press Absorbance Check in the Optical Checks menu.

2. Press λ to input the wavelength.

3. Input the wavelength and press OK to confirm.

4. Remove any cuvette/sample cell from the cell compartment,

close the cell compartment, and press Zero.

5. Insert sample cuvette/cell or test filter into the cell

compartment, close the cell compartment, and press Read.

6. Repeat steps 4 and 5 for a total of 10 replicates to obtain final

results.

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7. The result is displayed and is to be compared with the

nominal/standard data of the test filter. If a specific sample is
used, the measured results must be evaluated against
user-defined requirements.

8. Press Cancel to return to Optical Checks.

6.8.3.5 Drift check

The Drift Check test is used to test the stability of the instrument.
Note: The Drift Check runs 1 hour.

1. Press Drift Check in the Optical Checks menu.

2. Press λ to input the wavelength.

3. Input the wavelength and press OK to confirm.

4. Press Start.

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5. The Drift Check runs 1 hour.

This test takes a reading every minute for one hour. Every
15 minutes, linear regression is used to calculate the slope
(rate of change) for the previous 15 minute interval.

6. The last reading and slope for each 15 minute interval are
displayed. At the end of the hour, the overall values are
calculated and displayed. The test proceeds to completion.

7. Press Cancel to return to Optical Checks.

6.8.3.6 Verification kit

The Verification Kit, LZV 537, (see Section 9 on page 129) is
designed for periodic monitoring of scattered light, photometric
accuracy and the wavelength accuracy of the spectrophotometers.
When results exceed allowable tolerances (given in the quality
control certificate), contact the manufacturer.

1. Press Verification Kit in the Optical Checks menu.

2. Press Nominal Values.

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3. Press Edit.

An automatic menu guidance queries values (filters,

wavelength, nominal values and tolerances) given in the quality
control certificate, to the following specifications:

• Stray Light
• Photometrical accuracy
• Wavelength accuracy

4. Press OK when all values are entered and the overview is

displayed.

5. Press Verification.

6. Insert the Multi Cell Holder in the cell compartment in such a

way that the cell opening for 10 mm square cuvettes/cells is at
the front. Secure the holder with two locking screws.

7. Remove any cuvettes/cells from the cell compartment, close

the cell compartment, and press Start.

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8. Insert the different filter in the given order one after the other
with the filter glass facing the front of the instrument. Press
Next after inserting a filter and closing the cell compartment

After the last measurement the results are displayed.

9. Press PC & Printer icon to send the data to a USB memory

stick, PC or to a printer.

The files will be stored automatically as CSV file (Comma

Separated Value). The file name will be formatted as
“Verification.csv“.

6.8.4 Output checks

If a printer is connected a test printing of the current screen will be
printed.

6.8.5 Lamp history

The Lamp History menu provides the following information:
• The amount of time that the lamp has been on (Hours)
• The number of times that the lamp has been turned on (Cycles)
• The number of times that the instrument tried to turn the UV
lamp on (triggers)

After a lamp is replaced and the Lamp History is reset, the display
of the total operating time is reset to 0.

1. Press Lamp History in the System Checks menu.

2. Press Reset VIS and the Visible Lamp will be reset.

3. Press Reset UV and the UV Lamp will be reset.

4. Press OK to return to System Checks.

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6.8.6 Factory service

The Factory Service menu is password protected. This menu is not

intended for customer use.

6.8.7 Service time

In order to ensure a regular inspection, an automatic memory
reference for the service times can be entered. After switching the
instrument on this memory reference will be activated and indicated
at the appropriate time.

1. Press Service Time in the System Checks menu.

2. Select On and then Last Service to enter the date of the last
inspection.

3. Press OK to confirm.

4. Select Next Service to determine a specific period of time up to

the next inspection.

5. Press OK to confirm.

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If the next service is due, the message "Next service is due!" is

displayed after switching on the instrument.

6. Press OK to return to the Main Menu.

Contact the manufacturer or distributor to arrange an appointment

for the next service.

6.8.8 Instrument Backup

Before the next service date the Instrument Backup menu offers
the possibility to store all programs, measuring data, Operator ID,
Sample ID, passwords and all adjustable data on a USB stick.

1. Press Instrument Backup in the System Checks menu.

2. Connect the USB memory stick (section 3.3 on page 14).

3. Press Store to start a Backup.

Note: If the USB stick is not connected, the message "Please insert USB
Memory" is displayed. Connect a USB stick, in order to store the data.
Press OK to confirm and press Store again.

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Note: If the Backup was already stored before, the message "Data already
exists. Overwrite?" is displayed. Press OK to overwrite the data.

If the file was stored the message Instrument Backup is stored to

USB stick will be displayed.

4. Press OK to return to the System Checks menu.

Restore backup data:

Important Note: All current data will be overwritten when restoring
the Backup file!

1. Press Instrument Backup in the System Checks menu.

2. Connect the USB memory stick containing the Backup

(section 3.3 on page 14).

3. Press Restore to pass back the data.

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Note: If the USB stick is not connected, the message "Please insert USB
Memory" is displayed. Connect a USB stick, in order to store the data.
Press OK to confirm and press Restore again.

4. Press OK to confirm after the message “Instrument Backup

from S/N XXXXXXX. Restore?“ is displayed.

5. After the backup start the instrument again.

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CAUTION
Potential Chemical, Biological Eye and Skin Hazards.
Only qualified personnel should conduct the tasks described
in this section of the manual.

Important Note: Remove any cuvettes/cells that are still in the

instrument and dispose of them or their contents using an approved
disposal method.

7.1 Cleaning requirements

CAUTION
Potential Pinch, Eye, Burn and Chemical Hazards.
Always disconnect power from the instrument before
attempting any cleaning operations.

Important Note: Under no circumstances should the instrument,

display or the accessories be cleaned with solvents such as white
spirit, acetone, etc.

7.1.1 Spectrophotometer
• Clean the enclosure, cuvette/sample cell compartments and all
accessories with a soft damp cloth. A mild soap solution can
also be used. Do not get excess water in the cuvette/sample
cell compartments. Do not insert a brush or sharp object into
Cell Compartment #1 to avoid damaging the mechanical
components.
• Dry the cleaned parts carefully with a soft cotton cloth.

7.1.2 Display
• Take care not to scratch the display. Do not touch the screen
with ball pens, pencils or similar pointed objects.
• Clean the display with a soft, lint-free and oil-free cotton cloth.
Diluted window cleaner liquid can also be used.

7.1.3 Cuvettes/sample cells

CAUTION
Potential Chemical/ Biological Exposure Hazards.
Use proper laboratory practices whenever there is a risk of
chemical exposure.

1. After performing a procedure, clean glass cuvettes/sample cells

with cleaning agents.

2. Afterwards, rinse the cuvettes/sample cells several times with

tap water and then thoroughly with deionized water.

Important Note: Glass cuvettes/sample cells that have been used

for organic solvents (such as chloroform, benzene, toluene, etc.)
must be rinsed with acetone before being treated with cleaning
agents. In addition, another rinse with acetone is necessary as a
final treatment step before the cuvettes/sample cells are dried.

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7.2 Lamp replacement

CAUTION
The UV lamp generates UV light. Do not look directly at an
operating lamp without wearing UV protective eye glasses.

The Lamp compartment is on the left side behind the display and is
provided with ventilation on the back side. The tungsten and
deuterium (UV) lamp are installed in the lamp compartment. On the
back side a fan is installed for cooling of electric components.
The ventilation system operates automatically.
Refer to section 7.2.1 on page 122, section 7.2.2 on page 123 and
Figure 8 on page 124 for instructions on replacing the tungsten
lamp and deuterium lamp (UV).

7.2.1 Tungsten lamp replacement

1. Switch the instrument off.

2. Unplug the power cord.

DANGER
The instrument must remain switched off until the change of
lamp has been completed.

WARNING
Burn Hazard. Wait until the lamp cools down. Contact with the
hot lamp can cause burns.

3. Use a screwdriver to remove the cover from the back of the

instrument (the screws may be slotted or cross-headed).

4. Place the cover and the attached fan carefully beside the
instrument (take special care with the fan cable).

5. Push the spring (Figure 8 on page 124, item 2) up and remove

the tungsten lamp (Figure 8, item 1) from the lamp
compartment.

6. Unplug plug contact (Figure 8, item 7) from the tungsten lamp.

Important Note: The lamp should only be held by the lamp socket.
Avoid touching the glass.

7. Push plug contact firmly onto the new tungsten lamp.

8. Insert the tungsten lamp into the lamp compartment. Push the
spring down.

9. Check that the spring and the lamp socket are positioned
correctly.

10. Use a screwdriver to screw the back cover onto the instrument
again.

11. Plug in power supply again.

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12. Switch the instrument on.

13. Reset the Lamp History, see section 6.8.5 on page 115.

7.2.2 Deuterium lamp (UV) replacement

1. Switch the instrument off.

2. Unplug the power cord.

DANGER
The instrument must remain switched off until the change of
lamp has been completed.

WARNING
Burn Hazard. Wait until the lamp cools down. Contact with the
hot lamp can cause burns.

3. Use a screwdriver to remove the cover from the back of the

instrument (the screws may be slotted or cross-headed).

4. Place the cover and the attached fan carefully beside the
instrument (take special care with the fan cable).

5. Unplug the deuterium lamp (Figure 8 on page 124, item 4) from

the socket by pushing down on the safety contact.

6. Use a screwdriver to unscrew the two fastening screws

(Figure 8, item 3) (the screws may be slotted or cross-headed)
out of the socket.

7. Holding the lamp socket, lift the deuterium lamp up out of the
lamp compartment (remove the complete unit, including the
cable).

8. Carefully insert the new deuterium lamp into the lamp

compartment.

Important Note: Do not touch the glass envelope on the new lamp.
If it is touched, clean with alcohol.

9. Screw in both fastening screws again until they are finger-tight.

10. Insert the deuterium lamp in the socket so that the safety
contact clicks into place.

11. Use a screwdriver to screw the back cover onto the instrument
again.

12. Plug in power supply again.

13. Switch the instrument on.

14. Reset the Lamp History, see section 6.8.5 on page 115.

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Figure 8 Lamp compartment

1 Tungsten lamp 5 Plug contacts for fan
2 Spring 6 Plug contact for deuterium lamp
3 Screws 7 Plug contact for tungsten lamp (with grip and cable)
4 Deuterium lamp (lamp socket)

7.3 Fuse replacement

DANGER
Certain electrical circuits within this equipment are protected
by fuses against over-current conditions. For continued
protection against a risk of fire, replace fuses only with the
same type and rating specified.

DANGER
Failed fuses are generally an indication of a problem with the
equipment. If fuse failure continues, contact a service
representative for instructions on how to return the equipment
for repair. Do not attempt to repair the equipment yourself.

1. Switch the instrument off.

2. Unplug the power cord to remove all power from the

instrument.

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3. Pull out the plastic base over the power cable socket.

4. Remove the defective fuse, see Figure 1 on page 14.

5. Insert new fuse (T 2 A H; 250 V)

6. Replace the plastic base.

7. Plug in the power cord.

8. Switch the instrument on.

7.4 Filter pad maintenance

To determine when the filter mat needs to be replaced, inspect the
filter mat every 3–6 months (in a relatively dust-free environment,
this interval can be longer).

1. Remove any cuvettes and cuvette/sample cell adapter from the

cell compartment.

2. Turn the instrument off.

3. Unplug the power cord.

4. Lift the instrument and check the color of the filter mat. Replace
the filter mat if is dark gray or black.

7.4.1 Filter pad replacement

5. Carefully turn the instrument over and place it on a soft surface.

6. Use a screwdriver (standard or cross-head) to open the

filter grid (Figure 9 on page 126, item 1).

7. Lift the filter grid (Figure 10 on page 126, item 1).

8. Remove the old air filter pad and replace it with a new air filter
pad (Figure 10, item 2).

9. Screw the grid back in place.

10. Carefully stand the instrument upright.

11. Plug the instrument in.

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Figure 9 Base of DR 5000 with filter grid

1 Filter grid 3 Phillips screw
2 Filter pad

Figure 10 Change of filter pad

1 Filter pad

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 Section 8 Troubleshooting

Problem/Display screen Likely Cause Action

Dilute the sample and repeat the
Absorbance > 3.5! The measured absorbance exceeds 3.5
measurement
Calculated concentration is higher than Dilute the sample and repeat the
Concentration too high!
999999 measurement
Error
Barcode controlnumber? Deviation to the stored data Data updating
Please update program data!

Error Data updating

Program not available. Barcode cuvette test missing
Please update program data!

Error The cuvette is soiled or there are Clean the cuvette; allow the particles to
Clean Cuvette undissolved particles in the cuvette settle

Error Check the lamp and replace it if

The lamp output is too low
Please check the UV Lamp. necessary

Error Check the lamp and replace, if

Selfcheck stopped. necessary.
Self Check Test stops while starting the
Please check the lamp.
instrument Close the lid.
Please close the lid.
Error [xx] Press Start Again.

Error Remove the cuvette/sample cell from

Selfcheck stopped. Self Check Test stops while starting the the cell compartment.
Please remove the cuvette instrument
Press OK.
Please close the lid.
Kink in tube
Error Sipper? Check the tube connections
Peristaltic pump failure
Error
Contact the manufacturer or a sales
Selfcheck stopped.
Electronic defect representative and indicate the error
Hardware error.
number
Error [x]

Negative result! The calculated result is negative Check the concentration of the sample
Check the programming
Error in the test database / user
No evaluation! Contact the manufacturer or a sales
database
representative
The measured absorbance is above the Dilute the sample and repeat the
Over measuring range
calibration range of the test measurement
If possible, select a test with a lower
The measured absorbance is below the
Under measuring range measurement range or use a cuvette
calibration range of the test
with a longer path length

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 Section 9 Replacement Parts

Description Cat.-No.
Sipper Module DR 5000 LZV485
Peltier temperature controlled Module DR 5000 LZV513
Pour-Thru Module DR 5000 LZV479
Multi-Cell Holder DR 5000 A23618
Carousel Holder (Sample changer) A23620
USB-Keyboard (keyboard layout: US) LZV582
USB-Barcode Scanner (handset scanner for entering sample IDs) LZV566
Hach Data Trans (PC software for data transfer) LZY274
Brewery Analysis Software LZV659
Sipper Tubing, Inlet and Waste A23800
Sipper Tubing, PHARMED quality LZV580
Sipper Tubing, comes standard with Sipper Module LZV780
Pour Thru Tube Kit LZV569
USB-Memory Stick 2946900
USB-Interface Cable, (1 m) LZV567
Tungsten (VIS) Lamp A23778
Deuterium (UV) Lamp A23792
Fuse A23772
Filter Pad A23766
Certified test filter set for self-checks (Verification Kit)
LZV537
(6 precision glass filters with target values)
Semi-Micro Cell, 50 mm, Glass LZP269
Macro Cell, 20 mm, Glass LZP331
Sample Cell, 1 cm Square Glass, (10 mm path length) matched pair 2095100
Sample Cell, 1 cm Square Quartz, (10 mm path length) matched pair 4822800
Sample Cell, 50 mm Rectangular Glass 2629250
Sample Cell, 50 mm Rectangular Quartz 2624450
Sample Cell, 100 mm Rectangular Glass 2629201
Sample Cell, 100 mm Rectangular Quartz 2624401
Sample Cell, 1" Round Plastic, (1 cm path length) 10 mL, w/cap 4864302
Sample Cell, 1" Square Glass, 10 mL, matched sets 2495402
Sample Cell, 1" Square Glass, 10&25 mL, matched sets 2665902
Sample Cell, 1" Square Plastic 2410212
Pour Thru Cell, 1x1 cm (Quartz/160 µL/ Glass center height 10 mm) A24209
Pour Thru Cell, 1x1 cm (Quartz/450 µL/ Glass center height 10 mm) LZV510
Pour Thru Cell, 5x1 cm (Quartz/370 µL/ Glass center height 10 mm) LZV649
Pour Thru Cell, 1" Square (1" path length) 5913700
Adapter for 100 mm Sample Cell LZY421
Dust Cover LZV728

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 Section 10 Contact Information
HACH Company Repair Service in the Repair Service in Canada: Repair Service in
World Headquarters United States: Hach Sales & Service Latin America, the
P.O. Box 389 HACH Company Canada Ltd. Caribbean, the Far East,
Loveland, Colorado Ames Service 1313 Border Street, Unit 34 Indian Subcontinent, Africa,
80539-0389 U.S.A. 100 Dayton Avenue Winnipeg, Manitoba Europe, or the Middle East:
Tel (800) 227-HACH Ames, Iowa 50010 R3H 0X4 Hach Company World
(800) -227-4224 Tel (800) 227-4224 Tel (800) 665-7635 Headquarters,
(U.S.A. only) (U.S.A. only) (Canada only) P.O. Box 389
Fax (970) 669-2932 Fax (515) 232-3835 Tel (204) 632-5598 Loveland, Colorado,
[email protected] Fax (204) 694-5134 80539-0389 U.S.A.
www.hach.com [email protected] Tel +001 (970) 669-3050
Fax +001 (970) 669-2932
[email protected]

HACH LANGE GMBH HACH LANGE LTD HACH LANGE LTD HACH LANGE GMBH
Willstätterstraße 11 Pacific Way Unit 1, Chestnut Road Hütteldorferstr. 299/Top 6
D-40549 Düsseldorf Salford Western Industrial Estate A-1140 Wien
Tel. +49 (0)2 11 52 88-320 GB-Manchester, M50 1DL IRL-Dublin 12 Tel. +43 (0)1 9 12 16 92
Fax +49 (0)2 11 52 88-210 Tel. +44 (0)161 872 14 87 Tel. +353(0)1 46 02 5 22 Fax +43 (0)1 9 12 16 92-99
[email protected] Fax +44 (0)161 848 73 24 Fax +353(0)1 4 50 93 37 [email protected]
www.hach-lange.de [email protected] [email protected] www.hach-lange.at
www.hach-lange.co.uk www.hach-lange.ie
DR. BRUNO LANGE AG HACH LANGE FRANCE HACH LANGE SA DR. LANGE NEDERLAND
Juchstrasse 1 S.A.S. Motstraat 54 B.V.
CH-8604 Hegnau 33, Rue du Ballon B-2800 Mechelen Laan van Westroijen 2a
Tel. +41(0)44 9 45 66 10 F-93165 Noisy Le Grand Tél. +32 (0)15 42 35 00 NL-4003 AZ Tiel
Fax +41(0)44 9 45 66 76 Tél. +33 (0)1 48 15 68 70 Fax +32 (0)15 41 61 20 Tel. +31(0)344 63 11 30
[email protected] Fax +33 (0)1 48 15 80 00 [email protected] Fax +31(0)344 63 11 50
www.hach-lange.ch [email protected] www.hach-lange.be [email protected]
www.hach-lange.fr www.hach-lange.nl

HACH LANGE APS HACH LANGE AB HACH LANGE S.R.L. HACH LANGE S.L.U.
Åkandevej 21 Vinthundsvägen 159A Via Riccione, 14 Edif. Arteaga Centrum
DK-2700 Brønshøj SE-128 62 Sköndal I-20156 Milano C/Larrauri, 1C- 2ª Pl.
Tel. +45 36 77 29 11 Tel. +46 (0)8 7 98 05 00 Tel. +39 02 39 23 14-1 E-48160 Derio/Vizcaya
Fax +45 36 77 49 11 Fax +46 (0)8 7 98 05 30 Fax +39 02 39 23 14-39 Tel. +34 94 657 33 88
[email protected] [email protected] [email protected] Fax +34 94 657 33 97
www.hach-lange.dk www.hach-lange.se www.hach-lange.it [email protected]
www.hach-lange.es

HACH LANGE LDA HACH LANGE SP.ZO.O. HACH LANGE S.R.O. HACH LANGE S.R.O.
Av. do Forte nº8 ul. Opolska 143 a Lešanská 2a/1176 Roľnícka 21
Fracção M PL-52-013 Wrocław CZ-141 00 Praha 4 SK-831 07 Bratislava –
P-2790-072 Carnaxide Tel. +48 (0)71 342 10-83 Tel. +420 272 12 45 45 Vajnory
Tel. +351 214 253 420 Fax +48 (0)71 342 10-79 Fax +420 272 12 45 46 Tel. +421 (0)2 4820 9091
Fax +351 214 253 429 [email protected] [email protected] Fax +421 (0)2 4820 9093
[email protected] www.hach-lange.pl www.hach-lange.cz [email protected]
www.hach-lange.pt www.hach-lange.sk

HACH LANGE KFT. HACH LANGE S.R.L. HACH LANGE HACH LANGE SU
Hegyalja út 7-13. Str. Leonida, nr. 13 8, Kr. Sarafov str. ANALİZ SİSTEMLERİ
H-1016 Budapest Sector 2 BG-1164 Sofia LTD.ŞTİ.
Tel. +36 (06)1 225 7783 RO-020555 Bucuresti Tel. +359 (0)2 963 44 54 Hilal Mah. 75. Sokak
Fax +36 (06)1 225 7784 Tel. +40 (0) 21 201 92 43 Fax +359 (0)2 866 04 47 Arman Plaza No: 9/A
[email protected] Fax +40 (0) 21 201 92 43 [email protected] TR-06550 Çankaya/ANKARA
www.hach-lange.hu [email protected] www.hach-lange.bg Tel. +90 (0)312 440 98 98
www.hach-lange.ro Fax +90 (0)312 442 11 01
[email protected]
www.hach-lange.com.tr

HACH LANGE D.O.O. ΗΑCH LANGE E.Π.Ε. HACH LANGE E.P.E.

Fajfarjeva 15 Αυλίδος 27 27, Avlidos str
SI-1230 Domžale GR-115 27 Αθήνα GR-115 27 Athens
Tel. +386 (0)59 051 000 Τηλ. +30 210 7777038 Tel. +30 210 7777038
Fax +386 (0)59 051 010 Fax +30 210 7777976 Fax +30 210 7777976
[email protected] [email protected] [email protected]
www.hach-lange.si www.hach-lange.gr www.hach-lange.gr

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 Section 11 Limited Warranty

Hach Company warrants its products to the original purchaser against any defects that are due to faulty
material or workmanship for a period of one year from date of shipment unless otherwise noted in the
product manual.

In the event that a defect is discovered during the warranty period, Hach Company agrees that, at its
option, it will repair or replace the defective product or refund the purchase price excluding original
shipping and handling charges. Any product repaired or replaced under this warranty will be warranted
only for the remainder of the original product warranty period.

This warranty does not apply to consumable products such as chemical reagents; or consumable
components of a product, such as, but not limited to, lamps and tubing.

Contact Hach Company or your distributor to initiate warranty support. Products may not be returned
without authorization from Hach Company.

Limitations
This warranty does not cover:
• Damage caused by acts of God, natural disaster, labor unrest, acts of war (declared or undeclared),
terrorism, civil strife or acts of any governmental jurisdiction

• Damage caused by misuse, neglect, accident or improper application or installation

• Damage caused by any repair or attempted repair not authorized by Hach Company

• Any product not used in accordance with the instructions furnished by Hach Company

• Freight charges to return merchandise to Hach Company

• Freight charges on expedited or express shipment of warranted parts or product

• Travel fees associated with on-site warranty repair

This warranty contains the sole express warranty made by Hach Company in connection with its
products. All implied warranties, including without limitation, the warranties of merchantability and fitness
for a particular purpose, are expressly disclaimed.

Some states within the United States do not allow the disclaimer of implied warranties and if this is true in
your state the above limitation may not apply to you. This warranty gives you specific rights, and you
may also have other rights that vary from state to state.

This warranty constitutes the final, complete, and exclusive statement of warranty terms and no person
is authorized to make any other warranties or representations on behalf of Hach Company.

Limitation of Remedies
The remedies of repair, replacement or refund of purchase price as stated above are the exclusive
remedies for the breach of this warranty. On the basis of strict liability or under any other legal theory, in
no event shall Hach Company be liable for any incidental or consequential damages of any kind for
breach of warranty or negligence.

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 Index

A M
Absorbance Check ................................................. 111 Main Menu ............................................................... 22
Absorbance Formula ......................................... 90, 91 Maintenance .......................................................... 121
Alphanumeric keypad .............................................. 21 Mark ....................................................................... 103
Audio signals ........................................................... 25 Measurement Process .................................54, 55, 72
B Measuring sequence ................................................ 73
Barcode Programs .......................................51, 52, 53 Multi Wavelength ............... 22, 64, 66, 90, 91, 92, 93
edit ..................................................................... 53 N
Basic data ................................................................ 42 Name ....................................................................... 54
C Noise Check ........................................................... 108
Calibration formula ................................................... 75 O
Calibration Settings .................................................. 66 Operating environment ............................................ 13
Chemical Form ............................................43, 47, 53 Operator ID .............................................................. 23
Chemical form .................................................... 51, 52 creating .............................................................. 23
Cleaning delete ................................................................. 23
Display ............................................................. 121 Optical Checks ...... 106, 107, 108, 110, 111, 112, 113
Spectrophotometer .......................................... 121 Output Checks ........................................................115
Concentration Factor ............................ 87, 88, 90, 92 P
Concentration Resolution ..................... 87, 88, 90, 92 Password ................................................................. 32
Continuous Reading .......................................... 43, 45 activate .............................................................. 32
Cursor Mode ...................................................... 94, 96 PC and Printer ................................................... 29, 31
Cuvettes/cells ........................................................ 121 Print data .................................................................. 31
D Printer Setup ............................................................ 29
Data Printing data ............................................................. 31
delete ........................................................... 35, 37 Program ................................................................... 61
recall .................................................................. 35 Programm overview ................................................. 22
send ................................................................... 35 R
store .................................................................. 35 Reading Mode ....................................... 43, 44, 88, 89
Data Storage ............................................................ 38 Reagent Blank ................................................... 43, 48
Date ......................................................................... 25 Recall Data ................22, 38, 43, 53, 88, 91, 95, 101
Date and Time ......................................................... 25 Reference Scan ................................................. 94, 99
Dilution Factor ..............................................43, 45, 53 S
Display ............................................................. 21, 121 Safety Information ...................................................... 9
Display and Sound ................................................... 25 Sample blank ........................................................... 53
Drift Check ..............................................................112 Sample ID ................................................................ 24
F create ................................................................ 24
Factory Service .......................................................116 delete ................................................................. 24
Favorites ............................................... 22, 49, 80, 81 Save as User Program .......................... 43, 53, 88, 90
delete ........................................................... 80, 81 Scale & Units .................................... 95, 97, 101, 102
edit ..................................................................... 80 Security List ............................................................. 32
recall .................................................................. 81 Select View .............................................................. 96
Filter Settings ........................................................... 36 Send Data .....................................36, 43, 53, 94, 101
Formula .............................................................. 54, 55 Service Time ...........................................................116
Free programming ..........................72, 73, 75, 78, 80 Single Reading ......................................................... 43
H Single Wavelength .......22, 43, 45, 63, 66, 87, 88, 89
HACH Data Trans .............................................. 31, 36 Software ........................................................... 58, 106
Hazard Information .................................................... 9 Standard Addition .............................................. 43, 82
I Standard Adjust ................................................. 43, 46
Instrument Backup ..........................................117, 118 Stored Data .............................................................. 35
Instrument Information ........................................... 105 Stored Programs ..........22, 42, 43, 45, 46, 47, 48, 49
Instrument Setup .......22, 23, 43, 53, 88, 91, 95, 101 send Data .......................................................... 43
Integral ............................................................... 94, 96 Stray Light Check ....................................................110
Interfaces ................................................................. 29 System Check .........................................................116
Internet ............................................................. 58, 106 System Checks 22, 105, 107, 108, 110, 111, 112, 113,
L ................................................................ 115, 116, 117
Language ................................................................. 19

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 T
Time & Interval ............................................... 101, 102
Time Course ..........22, 38, 39, 40, 41, 101, 103, 104
Time Course Scan Reading ................................... 102
Timer .................................. 25, 43, 53, 54, 56, 87, 90
Touch Screen .......................................................... 21
U
Unpack the instrument ............................................. 13
Update .......................................................54, 58, 106
User Programs ................................................... 22, 61
V
Variables ......................................................54, 56, 78
Verification Kit .........................................................113
Version ............................................................... 54, 55
View Table ............................................................. 101
W
Warranty ................................................................ 133
Wavelength ........................................................ 87, 90
Wavelength Check ................................................. 107
Wavelength Range .................................................. 95
Wavelength Scan .........22, 38, 39, 40, 41, 94, 95, 98
Wavelength setting ................................................ 101
Wavelength spectrum .............................................. 94

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