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Lab 7 - Food Test (Digestion lab)

The document outlines a lab experiment aimed at testing food samples for reducing sugar, starch, lipids, and protein using various reagents and methods. It details the apparatus needed and the step-by-step procedures for each type of test, along with a section for recording observations and results. The document also includes sections for discussing biochemical reactions, sources of error, precautions, and reflections on the experiment.

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zairaheadley2009
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2 views

Lab 7 - Food Test (Digestion lab)

The document outlines a lab experiment aimed at testing food samples for reducing sugar, starch, lipids, and protein using various reagents and methods. It details the apparatus needed and the step-by-step procedures for each type of test, along with a section for recording observations and results. The document also includes sections for discussing biochemical reactions, sources of error, precautions, and reflections on the experiment.

Uploaded by

zairaheadley2009
Copyright
© © All Rights Reserved
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Digestion lab

Title: Food test


Aim: To test food samples for reducing sugar, starch, lipids and protein.
Apparatus and Materials:
Bunsen burner
Test tubes
Beaker
Glucose
Benedict’s solution
Biuret’s reagent
Ethanol
Water Bath
Food Test Samples

Method:
Test for reducing sugar
1.) A sample of food was crushed and placed in a test tube with water.
2.) A test tube of water was also placed on the rack
3.) 2cm3 of Benedict’s solution was added.
4.) Place both test tubes in a water bath
5.) The mixture was shaken and the observation was recorded

Test for Starch


1.) A potato was crushed and placed into a test tube
2.) A test tube of water was also placed on the rack
3.) 4 drops of Iodine solution was added to both test tubes
4.) The mixture was shaken and the observation was recorded

Test for lipids


1.) A sample of food was crushed and placed in a test tube
2.) A test tube of water was also placed on the rack
3.) 2cm3 of ethanol was added
4.) The mixture was shaken vigorously
5.) 2cm3 of water was added
6.) The observation was recorded

Test for protein


1.) A sample of food was crushed and placed in a test tube
2.) A test tube of water was also placed on the rack
3.) An equal volume of potassium hydroxide solution was added
4.) Two drops of biuret reagent was added
5.) The observation was recorded

Results:

Food tested Observation Inference

Reducing sugar

Starch

Lipids

Protein
Discussion
State the inference and observation seen for each test done. Explain the biochemical reactions
occuring in each test which caused the colour changes seen.
Example:
The test for reducing sugars produced a coloured precipitate with Benedicts solution. In the
Benedict's test, a reducing sugar reacts with the copper ions (Cu2+) in Benedict's reagent,
causing the copper ions to be reduced to cuprous ions (Cu+) which then precipitate out as a
brick-red colored copper(I) oxide, indicating the presence of a reducing sugar.

Sources of error (find 2)

Precautions

Conclusion

Reflection

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