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Streaking_Protocol_LS1102

The Streaking Protocol is a method for isolating pure strains of microorganisms, particularly bacteria, using sterile tools and aseptic techniques. It involves preparing an LB agar plate, sterilizing an inoculation loop, and streaking a colony onto a new plate for culture growth. The process includes specific steps for cleaning, labeling, and incubating the plates to ensure successful isolation and observation of the microorganisms.

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5 views

Streaking_Protocol_LS1102

The Streaking Protocol is a method for isolating pure strains of microorganisms, particularly bacteria, using sterile tools and aseptic techniques. It involves preparing an LB agar plate, sterilizing an inoculation loop, and streaking a colony onto a new plate for culture growth. The process includes specific steps for cleaning, labeling, and incubating the plates to ensure successful isolation and observation of the microorganisms.

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ada359413
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Streaking Protocol

Streaking is a technique used to isolate a pure strain from a single species of microorganism,
often bacteria. Samples can then be taken from the resulting colonies and a microbiological
culture can be grown on a new plate so that the organism can be identified, studied, or tested.
Streaking is done using a sterile tool, such as a cotton swab or commonly an inoculation loop.
Aseptic techniques are used to maintain pure cultures and to prevent contamination of the
growth medium. If the agar surface grows microorganisms which are all genetically same, the
culture is then considered as a pure culture. The modern streak plate method, was first
developed in Robert Koch's laboratory. It was first demonstrated by Loeffler and Gaffky in
Koch’s laboratory.

Requirements:- LB Agar Plate, Laminar Air Flow Cabinet, Inoculation loop, Ethanol, Spirit
lamp.
Procedure:-
• Before starting the work, clean the Laminar Air Flow Cabinet and give UV for 15 min.
• Before starting, clean your hands with 70% ethanol.
• After that, sterilize the inoculating loop by heating it with a spirit lamp. Wait for some
time to cool it, and touch the loop to the agar surface to check if it is cool.
• Label the petri dish with all important information, such as your name, date, media used
and the culture being inoculated.
• After the cooling, pick a colony of interest from the previously streaked plate and streak
it into a new agar plate.
• Place the plates in the incubator (overnight) at 37oC upside down to prevent moisture
loss.
• Record your observation the next morning (after 12 hours).

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