Operation manual of RePure-A

Note: The Hangzhou Bio-gener Technology Co., Ltd. reserves all rights
to modify this manual at any time without notice.

Please read this manual carefully before operating Thermal Cycler !

Company Name: Hangzhou Bio-Gener Technology Co., Ltd.

Company Add: B1, No.588 Weishan Road, Chunjiang Street, Fuyang District, Hangzhou

311421, China

Tel: 86-571-88992477

Fax: 86-571-88037572

Postal code: 311421

Email: [email protected]

Website:www.bio-gener.com
Document Version: July, 2024, Version 1.2.0

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 Operation manual of RePure-A

CONTENTS
CONTENTS ...................................................................................................................... - 3 -
Chapter 1 Safety Guidelines ......................................................................................... - 4 -
Chapter 2 Installation .....................................................................................................- 5 -
2.1 Package Contents Verification ............................................................................- 5 -
2.2 Normal Operating Conditions ............................................................................. - 5 -
2.3 Transportation and Storage Requirements ........................................................ - 5 -
2.4 Power Requirements .......................................................................................... - 5 -
Chapter 3 Instrument Characteristics ............................................................................- 6 -
3.1 Instrument Construction ......................................................................................- 6 -
3.2 Specialty ..............................................................................................................- 8 -
Chapter 4 Operation Instruction .................................................................................. - 10 -
4.1 Power on ........................................................................................................... - 10 -
4.2 Menu Structure ..................................................................................................- 11 -
4.3 File Instructions ................................................................................................. - 11 -
4.3.1 Create or Edit File ...................................................................................- 12 -
4.3.2 Delete File ...............................................................................................- 15 -
4.3.3 Copy File .................................................................................................- 15 -
4.3.4 Rename File ........................................................................................... - 15 -
4.3.5 Run File .................................................................................................. - 15 -
4.4 Setting ............................................................................................................... - 17 -
4.4.1 Running Setting ...................................................................................... - 17 -
4.4.2 System Setting ....................................................................................... - 18 -
4.4.3 E-mail Setting ......................................................................................... - 18 -
4.4.4 GLP Report .............................................................................................- 19 -
4.4.5 Device Information ................................................................................. - 19 -
4.4.6 Software Update .....................................................................................- 20 -
4.4.7 Tm Calculator ......................................................................................... - 20 -
4.4.8 Calibration Log ....................................................................................... - 20 -
4.5 Incubate ............................................................................................................ - 20 -
4.6 Log In ................................................................................................................ - 21 -
4.6.1 New User ................................................................................................ - 22 -
4.6.2 Change Password and Delete User ....................................................- 22 -
Chapter 5 Maintenance and Troubleshooting .............................................................- 23 -
5.1 Machine Maintenance .......................................................................................- 23 -
5.2 Troubleshooting ................................................................................................ - 24 -
5.3 Notes ................................................................................................................. - 24 -
5.4 Error Messages and Correspond Solutions ..................................................... - 25 -
5.5 Cause of the Abnormal Phenomenon and Correspond Solution .................... - 25 -
Chapter 6 After-sales Service ......................................................................................- 26 -

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 Operation manual of RePure-A

Chapter 1 Safety Guidelines

1. Instrument Safety Warning Labels

Icon Meaning
High temperature warning:
To avoid burning, please don’t touch directly all areas labeled with safety warning and
the hot areas described in manual.
Electric shocks Warning:
To avoid electric shock accidents, please operate strictly according to the request of
electric shocks warning.
Note:
Please note that reminder contains important information and be sure to read them
carefully. Otherwise it will cause the instrument to not work properly or even damage
the instrument.

2. Safe Use

Please read the following information before using the instrument and be sure to comply with the
following basic security measures. Failure to follow the measures or the other warnings in the
manual will affect the normal work of the instrument, even damage the equipment and hurt people.
1) Prohibit using the instrument in humid, dusty, high temperature, magnetic environment.
2) Prohibit opening the cover of instrument or touching the inner device of instrument.
3) Prohibit blocking the air flow vents and be aware of gloves or rags sucked into air flow vent in the
bottom of instrument.
4) Please keep instrument clean, and maintain it regularly.

Note: Please disconnect the power immediately and contact the supplier or consult certified
mechanic for assistance when any of the following occurs.
1) Instrument be moistened by rain or water, or any other liquid.
2) Instrument can’t work properly, especially any abnormal sound or odor appears.
3) Instrument’s function changes obviously.

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 Operation manual of RePure-A

Chapter 2 Installation
2.1 Package Contents Verification
Please open box and check the following list when you receive the RePure-A PCR instrument.
Name Quantity
Instrument 1

Power line 1

Spare fuse (8A , 250V) 2

Operation manual 1

Performance test table 1

In the event of discrepancy, please keep the original box and contact with us immediately.

2.2 Normal Operating Conditions

1) Used indoors.
2) Temperature 10℃-30℃.
3) Relative Humidity 10%-85%.
4) Please keep it stay away from heat sources to avoid liquid soaked in the instrument.
5) Do not block the air flow vent in the side and bottom of the instrument, and keep the air flow
vents of the instrument smoothly.

2.3 Transportation and Storage Requirements

1) Ambient temperature: -20℃- +55℃.
2) Relative humidity ≤90%.

2.4 Power Requirements

The instrument has switching power supply with PFC function. The range of writable voltage is
wide, it can run normally during voltage AC 100-240V and frequency 50-60HZ, and it must be
fitted with a reliable grounding.

Warning: To avoid electric shock accident, instrument must be fitted with a reliable grounding.

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 Operation manual of RePure-A

Chapter 3 Instrument Characteristics

3.1 Instrument Construction
A.The front of the machine

Status LED

Inner lid Handle

Lock column

Reaction block

LCD display

Air vents
USB A port

Figure 1. Frontal view of the thermal cycler.

● Lock column - locks hot lid

● Status LED - indicates status of reaction module
● Inner lid - maintains the lid temperature to prevent condensation and evaporation
● Seal washer - sealed reaction zone
● Reaction block - holds reaction vessels, including tubes
● Air vents - allows the thermal cycler to cool quickly
● LCD display - displays operating status
● USB A port - connects to a USB key, computer mouse, or other USB devices, for update
UI software

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 Operation manual of RePure-A

B. The back of the machine

Cooling fins

Air vents
Main board fan

Power switch Power input Air vents

Figure 2. Back view of the thermal cycler.

● Cooling fins - allows the thermal cycler to cool quickly

● Air vents - allows the thermal cycler to cool quickly
● Main board fan - allows the main board to cool

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 Operation manual of RePure-A

3.2 Specialty
1. Long service life Peltier heating units.
2. Reinforced aluminum module with anodizing technology can keep rapid heating-conducting
property and have enough corrosion resistance.
3. High heating and cooling rate, max. ramping rate 5 ℃/s, can save your precious time.
4. Self-adapting pressure hot lid makes closing lid and tightening lid in one step.
5. Air channel is in front and back and it allows machine placed side by side.
6.It has Android operation system and 10.1 inch capacitive touch screen. It has graphical menu
navigation interface and operation is very simple.
7. Built-in multiple standard program file template, can quickly edit the required files.
8. The running program and left time can be displayed in real time , allow to edit file when
program is running.
9. One-click quick incubation function can meet experiment’s needs such as denaturation,
enzyme cutting/enzyme-link and ELISA.
10. Hot lid temperature and hot lid work mode can be set to meet different experiment’s need.
11. Automatic restart after power failure. When power is restored it can continue to run
unfinished program.
12. Support USB to store and copy PCR data, user can control PCR by USB mouse.
13. Update software by USB flash.
14. Wi-Fi module built-in, one unit can control multiple PCR machine through computer or cell
phone with internet connection.
15. Email notification after experiment is over.

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3.3 Performance

Model RePure-A
Capacity 96×0.2ml
Reaction Volume 5-100μl
Temperature Range 0-105℃
Max. Heating Rate 5℃/s
Uniformity ≤±0.2℃
Accuracy ≤±0.1℃
Display Resolution 0.1℃
Temperature Control Block/Tube
Ramping Rate
Adjustable 0.01-5℃/s
Gradient Temp.
Range 30-105℃
Gradient Spread Horizontal: 0.1-42℃
Hot Lid Temperature 30-115℃
Number of Programs 200,000+(USB FLASH)
Max. No. of Step 30(Multiple nested PCR experiments)
Max. No. of Cycle 200(Up to 100,000 in nested cycles)
Time Increment/
Decrement 1 Sec-600 Sec, Long PCR is available
Temp. Increment/
Decrement 0.1-10.0℃, Touchdown PCR is available
Pause Function Yes
Auto Data Protection Yes
Hold at 4℃ Forever
Record hot lid and module temperatures in real time,
Temperature Curve
with a 60-minute recording time on a single page and a sliding view.
Record the running information of each step of the program,
GLP Report
and can export CSV, PDF format report.
File Encryption
Individual program files can be encrypted
Function
User Management
Three levels of access (administrator, user, guest)
Privilege
Email Notification Email notification after experiment is over
Lock Screen Prevents accidental termination of the program being experimented
Function on.
PC-LINK Function Simultaneous on-line monitoring of more than 250 instruments
Mobile Phone APP Simultaneous on-line monitoring of more than 250 instruments
Communication
USB2.0, LAN, WIFI
Interface
LCD 10.1inch, 1280×800 Pixels,
Dimensions 385mm× 270mm× 255mm (L×W×H)
Weight 10kg
Power Supply 100-240VAC, 50/60Hz, 600W

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 Operation manual of RePure-A

Chapter 4 Operation Instruction

4.1 Power on
Connect power cord to the back of instrument then plug into power source and switch to "-". .
When the instrument is on, the buzzer should be heard and the LCD screen lights up to show
the Boot screen (Figure 3). After that, the operating system starts up and then goes to the
Self-Test screen (Figure 4).During this phase, the LCD screen displays the product name,
company LOGO. When it’s over, the system performs the Home screen (Figure 5) as shown
below.

Figure 3. The boot screen. Figure 4. The self-test screen.

Figure 5. The home screen.

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4.2 Menu Structure

Main
menu
New File
Gradient
Home Ramp Rate
Advanced Touch Down
Long PCR
Clear All
Save As

File Library

Home
New Directory
Directory Option
File Option
Edit

Incubate
Settings
Running Options
GLP Report
System Log
Look Screen
TM Calculator
Calibration Log
Time and Time Zone
Sound Settings
WIFI Connection
Software Updates
Firmware Updates
Switch Language
Factory Mode
Email Alerts
Remote Access
Device Information
Modify Background
User

Home
Logout
Login
Registered User
Change
Password
Delete User

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4.3 File Instructions

File is composed by Temperature Step and Cycle Step. Each File can hold up to 30 Steps,
Temperature Step contains Temperature, Time, Gradient, Ramp Rate,Temperature
Increment opening point, Time Increment opening point, Temperature Increment and
Time Increment. The maximum number of Cycles is 200 times.

4.3.1 Create or Edit File

Touch “New File” button in the Home screen to create new file (Fig. 1).

Figure 1. Create a new file

Or in the Home screen, touch “File Library” button to enter File Library screen (Fig 2),then
Select a file in the File Library screen, touch “Edit” button to enter Edit File screen (Fig 3).

Figure 2. File Library

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 Operation manual of RePure-A

Figure 3. Edit file

1) Change Target Temperature and Hold Time

In the New File screen, touch “+Temp” button to add a Temperature Step, touch “+Cycle” button
to add Cycle Step (GOTO step), use the pop-up numeric keypad to enter a new value for the
target temperature or hold time.
This program edit uses a GOTO structure where the total number of cycles = the
number of repetitions of the GOTO step + 1.

2) Run mode

There are two run modes: standard mode and fast mode. Users can choose the mode
according to their actual needs. The fast mode takes less experimental time than the standard
mode.

3) Advanced

Select a desired step, touch “Advanced” button to enter Advanced screen (Figure 4).
Parameters can be edited by selecting among Gradient, Ramp Rate, Temperature Increment or
Time Increment. After entering Gradient values the gradient distribution table will be shown at
bottom of the screen. Touch “OK” to save changes and return Edit File screen (Figure 5).

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 Operation manual of RePure-A

Figure 4 Advanced

Figure 5. Set the Gradient

4) Save file

In the Edit File screen , select the file name edit box, input volume of reagent , lid temperature,
as shown in Fig. 6, input the "Save as" button after completion, select the save path as shown in
Fig. 7, or click the "Run" button to run the file directly.

Figure 6. Save files

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 Operation manual of RePure-A

Figure 7. Select path

4.3.2 Delete File

In the File Library screen, touch File Option button to select the file to delete, and the query
dialog window will pop up when touching “Delete file” button, and delete this file by touching
OK(Fig. 8). Only one delete per time is allowed to prevent any files being mistakenly deleted.

Figure 8. Delete a file.

4.3.3 Copy File

In the File Library screen, touch File Option button to select the file to copy and touch Copy file
button. Then enter the save path and touch Paste file button to finish copy.
4.3.4 Rename File

In the File Library screen, touch File Option button and select the file to rename. Then touch
Rename file button and enter new file name in the dialog window to rename files.
4.3.5 Run File

In the File Library screen, select the file needed and touch Run button or touch the "Run" button
on the new file/edit file screen to pop up the prompt dialog box. Then press the "OK" button to

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 Operation manual of RePure-A

enter the running file screen,Click the button on the right to switch to the next page (Fig. 9).

Figure 9. File running.

1) Parameter
● Start Time - system time when file starts to run.
● Remain Time - remaining time for the experiment.
● Lid Temp - current hot lid temperature.
● Step - current step.
● Step Time - current step time.
● Block Temp - current block temperature.
2) Pause
Touch “Pause” button to pause running file and touch “Resume” button to restore running.
3) Stop
Touch “Stop” button to stop a running file.
4) Skip
Touch “Skip” button to jump to next step.
5) Go to another screen

Touch Home button to back the Home screen, or touch Circle in the middle of the screen to
enter the running file screen (Fig. 10).

Figure 10. The file is running of home screen.

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 Operation manual of RePure-A

4.4 Setting
In the Home screen, touch Setting button to enter the Setting screen (Figure 11).

Figure 11. Setting menu

4.4.1 Running Setting

In the Setting screen, select the Running options to enter the Running setting screen (Fig. 12).
1) Hot lid temperature
Hot lid temperature setting range:30-115℃,The system default:105℃.
2) Hot lid work mode setting
In total there are 2 working modes and default mode is that the hot lid will be on when file
running.
3)Temperature control mode
The temperature control mode contains block control mode and tube control mode. The block
control mode is suitable for performing normal PCR and the tube control mode is for
experiments that require higher environment conditions. The system default is tube control
mode.

Figure 12. Running setting.

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4.4.2 System Setting

In the Setting screen, can set time and date, language, sound.
1) Time and date
Set the system time and date(Fig. 13).
2) Sound
● Keyboard sound- sound when touch button.
● Alarm sound- sound when system error occurs.
● File end sound- sound when file running is complete.
● Temp to reach sound- sound when the target temperature reaches.

Figure 13. Time setting.

4.4.3 E-mail Setting

In the Setting screen, select E-mail alerts to enter the E-mail Setting screen.
1) Status of email alert
After program is over it will automatically send email if chose Enable.
2) Test email
Test email function is to test whether email can send or not.
3) Save
Touch “OK” to save information and email setting record.

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 Operation manual of RePure-A

Figure 14. E-mail setting.

4.4.4 GLP Report

Record of every file name, time of start run this file, block number, source files path (Fig. 15).
● GLP Report- Record the time and temperature for each step.

Figure 15. GLP Report.

4.4.5 Device Information

Show the machine name(can modify), the SN., the control version and UI version (Figure 16).

Figure 16. Device information.

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 Operation manual of RePure-A

4.4.6 Software Update

Copy the new software to the root directory, insert the USB flash disk into the USB port, then
touch “Software update”, return to Android desktop when updating, and immediately reopen the
updated App .

4.4.7 Tm Calculator

Touch the fields to enter concentrations and the primer sequences,then touch Tm calculator (Fig.
17) to calculate the melting temperatures.

Figure 17. Tm Calculator.

4.4.8 Calibration Log

Click the "Calibration Log" button in the setting interface, as shown in Figure 18.

Figure 18. Calibration Log.

4.5 Incubate
In the Home screen, touch Incubate button to enter the Incubate wizard screen (Fig. 19).

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 Operation manual of RePure-A

Figure 19. Incubate.

In the Incubate wizard screen, select the desired block and enter the block temperature and the
hold time. Then touch Start button to enter Incubate Running screen (Fig. 20).

Figure 20. Incubate running.

4.6 Log In
In the Home screen, touch User button to enter the User screen (Fig. 21).
NOTE: Administrator user's initial password is “123456”, you can change the password
after logged in.

Figure 21. User management.

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4.6.1 New User

In the User screen, touch New user button to enter the New User screen (Fig. 22). Enter user
name and password, then touch OK button to enter the User screen. In the User screen,select
the Registered user who needs to log in and press the "Login" button(Fig. 23).

Figure 22. New user.

Figure 23. Login

4.6.2 Change Password and Delete User

Only after login can passwords be changed and users be deleted, can other users be deleted
from the login administrator account.

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 Operation manual of RePure-A

Chapter 5 Maintenance and Troubleshooting

5.1 Machine Maintenance
1) Regular cleaning

A. Clean the holes on reaction block with neutral soap solution (Do not use any solvent
containing strong alkali, strong alcohol and organic solution)
B.Spaces near the left, right air vents and under the machine should be always kept free. It is
very important to clean the dust around air vents regularly.
C. Clean the reaction block regularly and remove any residue inside cavities to prevent any
affects to the temperature control (we suggest to use soft cloth).

2) Replace fuse
The machine has two fuses. Replace it according to the following once damage occurs.

A. Turn off the machine and unplug power source.

B. Unscrew the fuse box with flat screwdriver and replace the damaged fuses with new 8A 250V
fuses. After replacement, screw back in the fuses box.

Note: Please contact your supplier for repair when encountering any problem replacing
fuses.

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 Operation manual of RePure-A

5.2 Troubleshooting
1) Dissatisfactory Results
Biological, programmatic and hardware problems may contribute to any unsatisfied results of
experiment. In order to distinguish the hardware problems from other possible problems, the
machine is equipped with built-in self-test hardware and self-diagnosis software. Below is the
detailed description. According to the experiences, most problems are related to biological and
programmatic factors.
FAQ as follows:
A. Incorrect or insufficient reactants.
B. Denaturation temperature is too high or too low. It is suggested to set the temp. within range
of 90-95℃ for 40 seconds. The duration can be adjusted in accordance with reaction volume.
C. Annealing temperature is too high or too low. It should be between 55-70℃ and 20 to 30
chains.
D. Reactant concentration is too high or too low.
E. Preparation process without special treatment.
F. Time and temperature value in progress are not appropriate.
G. The temperature of sample is slightly too low while the temp. of block is slightly too high.
H. Check whether the PCR tubes are well placed. Smearing little mineral oil on the surface of
holes will increase the thermal conductivity.
2) Machine’s self-test and self-diagnosis function
The machine will run the self-test program when booting. The software and hardware of
machine will show the results so as to inform users the potential problems, minimize the failure
of experiment and display the error message when problem occurs.

5.3 Notes
1) Power
A. No special requirement for the power supply. Any AC power source within range of 100-240V
is applicable. However, to prevent from causing any damage to the machine, it is better to apply
low voltage fluctuation power sources, otherwise consider to install power supply regulator.
B. It’s prohibited to cut off the power to terminate a running experiment. It’s very harmful to the
machine.
2) LCD screen
Avoid using UV disinfection to clean the machine to prevent any damage.
Avoid any bump or scratch to the LCD screen while using.
3) Note about cleaning
Avoid any liquid getting inside the machine while cleaning the base of machine. Due to the
possible usage of radioactive substance during experiment, please carefully handle cleaning. It
is not suitable to use the machine in moisture or hot environment.

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 Operation manual of RePure-A

Note: Please do read the notes carefully! You may damage the machine if don’t operate
according to the above requirements.

5.4 Error Messages and Correspond Solutions

No. Error message Cause and correspond solution

1 File name can not be empty File name does not support null character
2 If have same file name, Please Don’t support multiple files with the same
re-name name’s file.
3 Block sensor 1, short circuit Hardware problem, need to repair
4 Block sensor 1, open circuit Hardware problem, need to repair
5 Block sensor 2, short circuit Hardware problem, need to repair
6 Block sensor 2, open circuit Hardware problem, need to repair
7 Block sensor 3, short circuit Hardware problem, need to repair
8 Block sensor 3, open circuit Hardware problem, need to repair
9 Radiator sensor short circuit Hardware problem, need to repair
10 Radiator sensor open circuit Hardware problem, need to repair
11 Lid sensor short circuit Hardware problem, need to repair
12 Lid sensor open circuit Hardware problem, need to repair
13 Power output short circuit Hardware problem, need to repair
14 Air flow vent is blocked or circuit problem, need
Module temperature too high
to repair if latter
15 Temperature of environment is too low or circuit
Module temperature too low
problem, need to repair if latter
16 Air flow vent is blocked or fan problem, need to
Radiator temperature too high
repair if latter
17 Temperature of environment is too low or circuit
Radiator temperature too low
problem, need to repair if latter
18 Lid temperature too high Circuit problem, need to repair
19 Block temperature rise fault Hardware problem, need to repair
20 Hot lid temperature rise fault Hardware problem, need to repair

5.5 Cause of the Abnormal Phenomenon and Correspond Solution

No. Description of problem Cause and correspond solution

1 Check whether plug inserted correctly and
No any display after opening the
power output has electricity , pull out the plug
machine
while turning off ,and check the fuse
2 Turn on, the machine start running The power was off before ending the last
according to the program program
3 The fan run fast some time, slow Normal.Fan is used dissipating heat while pump
sometimes is working, not for reaching the sited temp.
4 Normal. When need large power for intense or
Have slight pat or squeaking sound cold, the switch power supply is adjusted
while machine is working automatically causing the sound of pat or
squeaking
5 Increasing or decreasing module Check whether the setting of rate of variable
temperature too slow temperature and fan is normal or not
6 Caused by electrostatic pulse or power surge.
Wrong display appears in the
please turn off and open, does not affect
screen
program running

Note: If the above problems can’t be solved, please contact the supplier in time.

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 Operation manual of RePure-A

Chapter 6 After-sales Service

1．This product is warranted under normal usage to the original purchaser for two years from
the date of purchase.
2．Lifetime service guarantee. The local dealer, maintenance station and regional offices will
provide you excellent after-sales service.
3．The foregoing maintenance free service is only applicable to products sold as new.
4．Free maintenance, product return and exchange shall not be provided under any one of the
following circumstances even in the warranty period:
1) Damage caused by natural disasters factors (such as fire, earthquake, flood, wind disaster,
thunderbolt etc.) and failure conditions caused by abnormal voltage, public nuisance and
chemical substance.
2) Failure or damage caused by use this product under abominable conditions (such as oil
fume, dust, damp and direct sunlight etc.) or inappropriate use according to the instruction book.
3) Damage caused by drop, move, transport, entry of foreign matter and other factors unrelated
to manufacture process.

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