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The document outlines various food tests to determine the presence of biological molecules such as glucose, starch, protein, and lipids in food samples. It provides detailed steps for preparing samples and conducting tests like Benedict’s test for glucose, iodine test for starch, Biuret test for proteins, and Sudan III test for lipids. Each test includes specific observations to identify the presence of these molecules.

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11 views11 pages

Study+guide+-+Chapter+2d+Biological+molecules+-+Food+tests+V3.0

The document outlines various food tests to determine the presence of biological molecules such as glucose, starch, protein, and lipids in food samples. It provides detailed steps for preparing samples and conducting tests like Benedict’s test for glucose, iodine test for starch, Biuret test for proteins, and Sudan III test for lipids. Each test includes specific observations to identify the presence of these molecules.

Uploaded by

ozdemirrdemirr
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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Structure, function &

organisation of living
organisms
Biological molecules - food tests
BIOLOGY Preparing samples
Food tests
We can do experiments (food tests) to check the content of any
food sample.

For example, does your fillet of fish contain:

● Glucose,
● Starch,
● Protein
● Fat
Food tests - Preparing samples
● Steps for preparing any food sample for
any food test:
1) Break up the food using a mortar &
pestle
2) Transfer the ground food to a beaker &
add distilled water
3) Stir the mixture with glass rod to
dissolve some of the food
4) Filter the solution using funnel (lined
with filter paper) to get rid of the solid
bits.
Structure, function &
organisation of living
organisms
Biological molecules - food tests
BIOLOGY Benedict’s test & Iodine test
Food tests 1: Glucose
Benedict’s test:
1) Transfer some pre-prepared food sample to a test tube.
2) Add several drops of Benedict’s solution using a pipette.
3) Place the test tube in a 75°C water bath & leave for 5 minutes.
4) Benedict’s solution starts as blue. If glucose present:
a) Low concentration: green or yellow
b) High concentrations: brick red
Food tests 2: Starch
Iodine solution test:

1) Transfer some pre-prepared food sample to a test tube.


2) Add a few drops of iodine solution and gently shake tube.
3) Iodine starts as orange-brown. If starch present it will turn
blue-black.

Negative Control Positive


Structure, function &
organisation of living
organisms
Biological molecules - food tests
BIOLOGY Biuret test & Sudan III stain
Food tests 3: Proteins
Biuret test

1) Transfer some pre-prepared food sample to a test tube.


2) Add an equal amount of Biuret solution to the sample. Mix
by gently shaking.
3) Biuret solution starts as blue. If protein present, it will turn
pink or purple.
Food tests 4: Lipids
Sudan III test
1) Transfer some pre-prepared food
sample to a test tube.
2) Add a few drops of Sudan III stain
solution to the test tube. Shake gently.
3) The solution will stain the lipids. If
lipids present, the solution will
separate into two layers. Top layer will
be bright red. If no lipids, no red layer.
Structure, function &
organisation of living
organisms
Biological molecules - food tests
BIOLOGY EXAM QUESTION
WALKTHROUGH
EXAM QUESTION WALKTHROUGH

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