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Validating micro pipette

The document provides a comprehensive guide for validating an automatic micropipette to ensure accurate liquid handling in laboratories. It includes steps for visual inspection, calibration checks using gravimetric and volumetric methods, and emphasizes the importance of regular maintenance and user training. Detailed documentation and environmental considerations are also highlighted to maintain the reliability of the micropipette's performance.

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7 views

Validating micro pipette

The document provides a comprehensive guide for validating an automatic micropipette to ensure accurate liquid handling in laboratories. It includes steps for visual inspection, calibration checks using gravimetric and volumetric methods, and emphasizes the importance of regular maintenance and user training. Detailed documentation and environmental considerations are also highlighted to maintain the reliability of the micropipette's performance.

Uploaded by

ikpefa hope
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOCX, PDF, TXT or read online on Scribd
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OCHONOGOR, IKEMEFUNE & OSIFO, JOY

Validating an automatic micropipette is essential to ensure accurate and precise liquid handling in laboratory
settings. Here’s a step-by-step guide to validate a micropipette:
1. Visual Inspection
• Check for Damage: Inspect the micropipette for any physical damage, such as cracks or wear on the tip ejector and
plunger.
• Cleanliness: Ensure that the pipette is clean and free from any residues.
2. Calibration Check
• Use Calibration Weights: For gravimetric calibration, use a balance to weigh the liquid dispensed by the
micropipette.
• Select Appropriate Liquid: Use distilled water at room temperature (20-25°C) for calibration, as its density is well-
known.
• Dispense Known Volumes: Set the micropipette to different volumes (e.g., 10 µL, 100 µL, 1000 µL) and dispense
into a pre-weighed container.
• Weigh the Dispensed Liquid: Record the weight of the liquid dispensed. Convert the weight to volume using the
density of water (1 g/mL).
3. Calculate Accuracy and Precision
• Accuracy: Compare the measured volume to the set volume. Calculate the percentage error using the formula:
Percentage Error = (( Measured Volume - Set Volume / Set Volum} )) × 100
• Precision: Repeat the dispensing process multiple times (at least 10 times) for each volume setting. Calculate the
standard deviation (SD) and coefficient of variation (CV) using:
CV = (( SD / Mean} )) × 100
4. Documentation
• Keep detailed records of all calibration checks, including date, results, and any corrective actions taken.
• Use a pipette calibration logbook or software for tracking.
5. Regular Maintenance
• Follow manufacturer guidelines for regular maintenance and servicing.
• Schedule periodic checks (e.g., quarterly or biannually) based on usage frequency.
6. User Training
• Ensure that all users are trained in proper pipetting techniques to minimize variability caused by user error.
7. Environmental Considerations
• Conduct validation in a controlled environment to minimize factors like temperature and humidity that can affect
results.
8. Use of Standards
• If available, use certified reference materials or standards to validate performance against known benchmarks.
By following these steps, you can ensure that your automatic micropipette is functioning accurately and reliably,
which is crucial for achieving consistent results in your laboratory work.

Micro pipettes are calibrated to ensure accurate and precise liquid aspiration and dispensing, to meet the
intended use
The process involves
Pre-Calibration Checks
1. Visual inspection: Check the pipette for any visible damage, wear, or corrosion.
2. Pipette maintenance: Ensure the pipette is properly maintained, including cleaning and lubricating moving parts.
Calibration Methods
1. Gravimetric calibration: This method involves measuring the weight of the liquid dispensed by the pipette. A
precise balance is used to measure the weight of the liquid.
2. Volumetric calibration: This method involves measuring the volume of the liquid dispensed by the pipette. A
precise volumetric measurement device, such as a burette, is used to measure the volume.
3. Photometric calibration: This method involves measuring the absorbance of a dye solution dispensed by the
pipette. A spectrophotometer is used to measure the absorbance.
Calibration Procedure
1. Prepare the calibration solution: Prepare a solution with a known density or concentration.
2. Set the pipette: Set the pipette to the desired volume.
3. Dispense the solution: Dispense the solution into a weighing dish or volumetric measurement device.
4. Measure the weight or volume: Measure the weight or volume of the dispensed solution.
5. Repeat the process: Repeat the process several times to ensure accuracy and precision.
6. Calculate the calibration factor: Calculate the calibration factor based on the measured values.
7. Adjust the pipette: Adjust the pipette as needed to ensure accurate and precise liquid handling.
Post-Calibration Checks
1. Verify the calibration: Verify the calibration by performing a series of tests.
2. Document the calibration: Document the calibration results, including the calibration factor and any adjustments
made to the pipette.
3. Schedule regular recalibration: Schedule regular recalibration to ensure the pipette remains accurate and precise
over time.

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