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Test type

The document outlines the testing procedures and result interpretations for three types of rapid HIV tests: One Step anti HIV (1 & 2), 1st Response HIV 1-2 card test, and Uni Gold HIV 1.2.0. It details the sample types, amounts, buffer requirements, timeframes, and expected results for each test, along with the interpretation of results as non-reactive, reactive, or invalid. The document serves as a job aid for public health laboratory personnel in conducting and interpreting rapid HIV tests.

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Gebremichael Reta Mengistu
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3 views

Test type

The document outlines the testing procedures and result interpretations for three types of rapid HIV tests: One Step anti HIV (1 & 2), 1st Response HIV 1-2 card test, and Uni Gold HIV 1.2.0. It details the sample types, amounts, buffer requirements, timeframes, and expected results for each test, along with the interpretation of results as non-reactive, reactive, or invalid. The document serves as a job aid for public health laboratory personnel in conducting and interpreting rapid HIV tests.

Uploaded by

Gebremichael Reta Mengistu
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOC, PDF, TXT or read online on Scribd
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Test type Sample Amount Buffer (Assay Time Result Interpretation

& Diluents)

R 2 lines (T & C) 2 lines of any intensity on both T & C

One step anti Whole Blood 1 Drop NR 1 line (C) 1 line appears in the C & No line in the T
HIV (1 &2)
(30µl) 1 Drop 10–20 Min Invalid No line appears Record the result of registration book but not
on C report invalid results. Repeat the test with a
Serum/Plasma new device.

Whole Blood 2 drops R 2(3) lines (T & C) 1 line should always appear in the control line
(vein/finger skin (20µl) region & another 1 or 2 colored line should
puncture 1 drop appear in the test T1 &/ or T2
1 st response 15-25 min
NR 1 line (C) 1 colored line appears in the C. No colored
HIV 1-2 card
lines appears in T1 &/ or T2
test 1 drop 1 drop
(10µl) Invalid No line appears Record the result of registration book but not
Serum/Plasma
on C report invalid results. Repeat the test with a
new device.

10‒12 min R 2(3) lines (T & C) T1 & C reactive for HIV‒1, T2 & C Reactive for
HIV‒2; T1, T2 & C reactive for HIV‒1 &/or HIV‒
Whole Blood 2
Uni Gold HIV 2 drops 2 drops
NR 1 line (C) 1 line appears in C & No line in T1 & T2
1.2.0
Serum /Plasma Invalid No line appears Record the result of registration book but not
on C report invalid results. Repeat the test with a
new device

Result Interpretation
One step anti HIV (1 &2) 1 st response HIV 1-2 card test One step anti HIV (1 &2) Uni Gold HIV 1.2.0 HIV Status

Non-Reactive Not Applicable Not Applicable Not Applicable Negative

Reactive Non-Reactive Reactive Not Applicable Appoint after 14 days

Reactive Reactive Not Applicable Reactive Positive

Reactive Reactive Not Applicable Non - Reactive Appoint after 14 days

SER PUBLIC HEALTH INSTITUTES LABORATORY JOB AID FOR RAPID HIV TEST NEW ALGORITHM ( ONE STEP, FIRST RESPONSE AND UNIGOLD)

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