TOCOBIOL SF C

Natural Antioxidant made up of mixed Tocopherols concentrate from natural NONGMO origin.

TOCOBIOL, E-306 …………………………………… 100%

INCI NAME Tocopherol (mixed), Beta-Sitosterol, Squalene
CAS NUMBER 59-02-9 / 16698-35-4 / 54-28-4 / 119-13-1 / 83-46-5 / 111-02-4
EINECS 200-412-2 / 240-747-1 / 200-201-5 / 204-299-0 / 201-480-6 / 203-826-1

CARRIER: Helianthus Annuus Seed Oil

STEROLS
Campsterol
11-13 %
Stigmasterol
14-17 %
B-Sitosterol
70-75 %

Last amendment: 27/11/2012, Rev 02

 Appearance: Oily liquid. Colour amber-yellowish to dark amber. It is possible
the presence of cereous components in microcrystalline form.
Solubility: Insoluble in water. Soluble in fats and oils.
Heavy Metals Not more than 10 ppm
Arsenic Not more than 3 ppm
Pesticides Absence

E.coli: Negative
Salmonella: Negative

Natural antioxidant especially appropriate for edible fats and oils, meat products, snacks,
essential oils and natural flavourings.

0,02-0,03% regarding the fat contents.

1, 2’5 and 5 Kg white polypropylene carafes in 20 Kg boxes, 10 kg carafes packaging.

We recommend storing the product in tightly closed containers under room temperature

Last amendment: 27/11/2012, Rev 02

conditions away from oxygen, heat and light.
Once open, use immediately.
Shelf-life: 2 years under the above mentioned storage conditions.

NON-GMO Certificate. KOSHER Certificate.

Detailed data upon request.

Last amendment: 27/11/2012, Rev 02

 LABORATORY REPORT

ANTIOXIDANT ACTIVITY OF TOCOBIOL IN COCONUT OIL

PROCEDURE

RANCIMAT METHOD at 98ºC and 120ºC

ANTIOXIDANTS TESTED

TOCOBIOL 200 ppm

BHA 200 ppm

200
150
120ºC
100
Horas
Ranci
mat

50 98ºC

0
Sin BHA
antioxidante

RESULTS

Comparison of induction times determined by Rancimat

The results obtained indicate that TOCOBIOL is the antioxidant that provides a greater
stability for Coconut Oil.

Assays performed at 98ºC show that TOCOBIOL increases this oil stability in 75%
while BHA samples show an increase of stability of 25%.

At 120ºC the induction time of the sample which contains TOCOBIOL is 32,8 hours.
That means an increase of the Coconut Oil stability of 6 test hours. The sample containing
BHA shows an induction time increase of 0,7 hours.

Last amendment: 27/11/2012, Rev 02

 LABORATORY REPORT
COMPARATIVE STABILITY STUDY OF BULK SAMPLES
STABILIZED WITH TOCOBIOL

Lab report #: 290 B Date: September 3rd 2008

AIM

Samples of lip stick mass containing different doses of TOCOBIOL will be tested using the
Rancimat method to determinate the protection against oxidation provided by the mentioned
Tocobiol doses.

Initial oxidation level of every sample will be tested measuring its Peroxide Value Index.

SAMPLES TESTED

Samples with the remarked doses of Tocobiol are received on August 13 th. They are named as
follows.

SAMPLE
TOCOBIOL amount
1A TOC A 78 0.000
2A TOC B 78 0.250
3A TOC C 78 0.500
1B TOC D 78 0.000
2B TOC E 78 0.250
3B TOC F 78 0.500
1C TOC G 78 0.000
2C TOC H 78 0.250
3C TOC I 78 0.500

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ANALYTICAL METHOD:

1. PEROXIDE INDEX

Peroxide value index determination is done under UNE 55.023 regulations

PEROXIDE INDEX
SAMPLE
(meq O2/ kg fat)
1A TOC A 78 (without antioxidant) 8.7
2A TOC B 78 5.4
3A TOC C 78 4.9
1B TOC D 78 (without antioxidant) 3.1
2B TOC E 78 2.0
3B TOC F 78 1.9
1C TOC G 78 (without antioxidant) 8.0
2C TOC H 78 7.8
3C TOC I 78 7.7

2. RANCIMAT METHOD

Rancimat test has been carried out over every sample to determinate the protection level
provided by Tocobiol at the used dosage.

Last amendment: 27/11/2012, Rev 02

RANCIMAT METHOD FOR “A” RANGE SAMPLES

TEMPERATURE: 120º C

AIR FLOW: 20 l/ h

CONDUCTIVITY RANGE : 200 µS

FAT MEDIUM: 1A , 2A , 3A SAMPLES

RESULTS:

Rancimat Stability
SAMPLE
hours increasing
1.- 1A TOC A 78 (WITHOUT ANTIOXIDANT) 5,15 h ---
2.- 2A TOC B 78 14,3 h 177,7%
3.- 3A TOC C 78 20,9 h 305,8%

Shown graphically:

"A" RANGE SAMPLES

25

20
Rancimat Hours

15

10

0
1A TOC A 78 (W.A.) 2A TOC B 78 3A TOC C 78

Last amendment: 27/11/2012, Rev 02

RANCIMAT METHOD FOR “B” RANGE SAMPLES

TEMPERATURE: 120º C

AIR FLOW: 20 l/ h

CONDUCTIVITY RANGE: 200 µS

FAT MEDIUM: 1B , 2B , 3B SAMPLES

RESULTS:

Rancimat Stability
SAMPLE
hours increasing
1.- 1B TOC D 78 (W.A.) 10,4 h ---
2.- 2B TOC E 78 30,1 h 189,4%
3.- 3B TOC F 78 51,0 h 390,0%

Shown graphically:

Last amendment: 27/11/2012, Rev 02

 "B" RANGE SAMPLES

60

50

40
Rancimat hours

30

20

10

0
1B TOC D 78 (W.A.) 2B TOC E 78 3B TOC F 78

RANCIMAT METHOD FOR “C” RANGE SAMPLES

TEMPERATURE: 120º C

AIR FLOW: 20 l/ h

CONDUCTIVITY RANGE: 200 µS

FAT MEDIUM: 1C , 2C , 3C SAMPLES

RESULTS:

Rancimat Stability
SAMPLE
Hours increasing
1.- 1C TOC G 78 (W.A.) 27,5 h ---
2.- 2C TOC H 78 41,2 h 49,8%
3.- 3C TOC I 78 51,2 h 86,2%

Shown graphically:

Last amendment: 27/11/2012, Rev 02

 "C" RANGE SAMPLES

60

50

40
Rancimat Hours

30

20

10

0
1C TOC G 78 (W.A.) 2C TOC H 78 3C TOC I 78

3. ORGANOLEPTIC CHARACTERISTICS

The samples sent by the customer show the following organoletic properties:

ORGANOLEPTIC
SAMPLE
PROPERTIES
1A TOC A 78 Pasty cream /
2A TOC B 78 Light green colored
3A TOC C 78
1B TOC D 78 Pasty cream /
2B TOC E 78 Yellow - grey colored
3B TOC F 78
1C TOC G 78 Greasy cream /
2C TOC H 78 Light yellow colored

Last amendment: 27/11/2012, Rev 02

 3C TOC I 78

CONCLUSIONS

Looking at the initial Peroxide Value index of the samples, we find that all mixes containing
TOCOBIOL, get better results than the one without it. That means that even from the
manufacturing time, Tocobiol provides a high level of protection against oxidation.

No difference in organoleptic properties are reported in the samples containing TOCOBIOL

regarding the ones without it.

Natural antioxidant TOCOBIOL gets better results when used over the most unstable samples
(A and B range) getting a stability increasing up to 300 % for A and 390% for B. The stability
improvement for C sample is lower (86%) because the Peroxide Value Index shows that its
oxidation level is lower. Sample C seems to be the more stable mix.

The adition of TOCOBIOL to every sample tested produces high increasings in the product´s
stability (up to 390% for B samples) without any organoleptical change.

Last amendment: 27/11/2012, Rev 02