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......-………..』
4 Animal Development . . . . . . ... .... ”·· ... ”· . 51
Fertilization . . . . . . . . . ... …........ ... 52
Influence
- i of Yolk on Cleavage Patterns . . ... …........ ... 52
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Sea Star Embryonic Development . . . . . ... .......... 54
Unfertilized Egg …………… 54
Fertilized Egg . . . . . . . . . . . . . . . . . 54
丁wo-Cell through Eight-Cell Stages . . . . . 54
Morula (16- 32 Cell Stages) . . . . . . . . . 54
Blastula (64+ Cell Stages) . . . . . . . . . . 54
Early Gastru la . . . . . . . . . . . . . . . . 55
Late Gastrula . . . . . . . . . . . . . . . . . 55
Bipinnaria Larva . . . . . . . . . . . . . . . 55
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Unfertilized Egg …………… 57
Ferti Iized Egg . . . . . . . . . . . . . . . . . 57
Early Cleavage . . . . . . . . . . . . . . . . 57
Late CI eavage . . . . . . . . . . . . . . . . 58
Blastu la . . . . . . . . . . . . . . . . . . . 58
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In Vitro Fertilization with Sea Urchins . . . . . . . . . . . . . . . . . . . 62
Precautions for Successful Development . . . 63
Obtaining Gametes . . . . . . . . . . . . . 63
Ferti Iization . . . . . . . . . . . . . . . . . 64
Cleavage . . . . . . . . . . . . . . . . . . . 66
Questions for Review . ………………··... ... 67
Laboratory Practical Challenge .. ……………........ ... 69
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Phylum Euglenozoa (Euglenoids, Kinetoplastids) ..........
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89
-
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Euglenoids (ex. Euglena) . . . . . . . . . . . 89
Kinetoplastids (ex. 开ypanosoma) . . . . . . 91
[ Exercise 6-2 [ Alveolata (Dinoflagellates, Apicomplexans, Ciliates) . . . . . . . . . . . 93
Dinoflagellates (Phylum Dinoflagellata) . . . 93
Apiζomplexans (Phylum Apicomplexa) . . . . 95
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Cil iates (Phylum Ciliophora) . . . . . . . . . 96
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Unikonta (Amoebas, Choanoflagellates, Animals, Fungi) . . . . . . . . . 100
-
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Amoebas . . . . . . . . . . . . . . . . . . 100
Choanoflagellates . . . . . . . . . . . . . 102
Questions for Review . . . . . . . ...... .......... .......... .. 103
Laboratory Practical Challenge . . ...... .......... ........... .. 105
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Observation of Spicules: The Sponge Skeleton . . . . 113
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Comb Jellies (Phylum Ctenophora) .. ... .. 138
[ Exercise 8-5 Investigating Cnidarian Feeding Behavior . . . . ..........
I .. 140
Using the Scientific Method . . . . . . . . . . . . . . . . . . . .......... . 142
Questions for Review . . . ...... .... . 147
Laboratory Practical Challenge . . . . . . . . . . . . .......... . 149
CONTENTS
9 Flatw。『’’n ..... ... ....... ... .. 151
Animal Body Plans . . . . . ··.... ...... …........ ... .. 152
Evolutionary
i - History . . . . . ..... ...... …... ..... ... .. 152
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Planaria (Class Turbellaria) ....... ..... .......... .. 154
Exercise 9-2 Flukes (Class Trematoda) ….....
I I ...... ..... ... .. 157
Sheep Liver Fluke . . . . . . . . . . . . . 159
Human Liver Fluke . . . . . . . . . . . . . 160
[以ercise 9-王J Tapeworms (Cla岱 Cestoda) .. …·.. ..... …... ... . . 161
Exercise 9-4 Investigating Flatworm Behaviors . . . . . ... ..... ...
I .. 165
Chemosensory and Feeding Behaviors . . . 165
Photosensory Behaviors . . . . . . . . . . 165
丁acti le Responses . . . . . . . . . . . . . . 167
A Simple Model for Learning . . . . . . . . 168
I Exercise 9-5 I Investigating Regeneration in Flatworms ................ .. 170
Questions for Review . . ... ... .. 173
Laboratory Practical Challenge .. ·... ... . . 175
[_;~~-r~]~~- !_
Q_-i_l Investigating Locomotion in the Vinegar Eel .... .... .. 185
Using the Scientific Method . . ... ... .. 187
际二rcise 10-31 Rotifers (Phylum Rotifera) . ... .... .. 189
Questions for Review . . . . . . . . . . . . . ... .......... ..... .. 193
Laboratory Practical Challenge . ... .......... ..... .. 195
CONTENTS
14 Echin。derm .............. ..... ... .. 289
Evolutionary History . . . . . . . . . . . . . . . . . . . . . . . 290
’
·四·
I Exercise 14-11 Sea Lilies, Feather Stars (Class Crinoidea) . . . . . . . . . . . . . . . 291
IExercise 14-2 I Brittle Stars (Class Ophiuroidea) . . . . . . . . . . . . . . . . 292
IExercise 14-3 I Sea Urchins, Sand Dollars (Class Echinoidea) ... ... .. 293
I Exe叫se 14-4 Sea Stars (Class Asteroidea) ...... ... ........ .. 294
Sea Star External Anatomy . . . . . . . 294
Sea Star Internal Anatomy . . . . . . . 296
I Exercise 14-5 I Sea Cucumbers (Class Holothuroidea) ··......... .. 300
IExercise 14-6 I Investigating Locomotion in Sea Stars and Sea Urchins . ... .. 302
Questions for Review . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 305
Laboratory Practical Challenge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 307
CONTENTS
21 Birds .. ... .. ..... ... .. 441
Evolutionary History . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 442 ’
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Appendix . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 533
References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 540
Glossary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 542
Photo Credits . ,. . . . . . . . . . . . . . . . . . . ,. . . . . . . . . . . . . . . . . 555
’‘
Index . . . . . . ’民 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 557
’民
afety guidelines in biology laboratories are often taken with a tongue-in-cheek approach,
but should be taken seriously. Many of you casually think,“What could happen to me
in zoo lab ?” Although you certainly don’t often run the risk of catching yourself on fire
or blowing up something (these risks are more akin to a chemistry lab), there are plenty
of avenues for accidents of all sorts in the zoology lab. Integral to learning about zoology
is accepting the responsibility that comes with doing zoology. In your pursuit of knowledge
about the animal kingdom, you will immerse yourself in observations, dissections, and
experiments, each with their own specific protocols, techniques, and inherent perils. Don’t
become complacent about the purported sanctuary of the zoology lab. Accidents can
happen-and will happen if you let down your guard.
A common complaint is that too many rules take the fun out of lab. Common sense
dictates that some degree of “ looseness ” must be sacrificed to gain the necessary level of
safety that will ensure a positive lab experience. The laboratory is no place for a carefree,
haphazard attitude. In the proper perspective, howeve乌 these basic guidelines will keep you
safer and happier and will teach you the appropriate protocols that allow zoologists to
study their organisms safely and effectively.
The following list of basic safety rules for the laboratory is offered as a guide to make
yo旧 laboratory experiences safe and enjoyable. It is by no means a complete list but,
rather, a starting point upon which the instructor can build a tailored list to suit your
specific laboratory. Remember-your best defense against accidents in the lab and your
greatest asset in dealing with situations when they arise is common sense. If that fails
you, alert your instructor!
XIII
• Know the locations of the first-aid kit and eyewash fountain in your laboratory and
know how to use them. Your instructor should discuss these safety items during the
first laboratory period. If they are not discussed, ask about them.
• Report any electrical anomalies to your instructor immediately (for example, frayed
electrical cords, bare wires, broken plugs, foreign objects in sockets, faulty switches).
• Alert your instructor in the event of an accident-no matter how harmless it seems!
There may be unseen dangers of which you are not aware.
• Report any contact with human blood to your instructor immediately.
• Clean your lab bench and other work surfaces at the end of each lab period.
• Wash your hands carefully with soap and warm wate乌 and rinse them thoroughly
before leaving the laboratory.
如5
ζH APTER
After completing the exercises in this chapter, you should be able to:
1 Understand metric weights and measurements. 4 Identify the parts of a stereoscopic microscope and
understand how to use it.
2 Identify the parts of aζompound microsζope and
understand how to use it. 5 Disζ阳
3 Make a wet mount of a specimen on a microscope slide. 6 Interpret 陀ferenζes to body symmetry, body planes,
and body regions of animals.
1
Metric Weights Materials Needed
and Measurements O metersticks
O metric rulers
Scientists throughout the world use a standardized system of weights and O staples
measurements, the metric system. This system has been adopted by virtually O graduated cylinders
every major country around the world with the exception of the United (SO ml, 100 ml, 1,000 ml)
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States. As a result, U.S. students often are not as familiar with the relation- CLU
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ships of metric units. Yet, the metric system has crept into our society in a --
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few areas (2-liter soda bottles, 35 mm cameras, 9 mm handguns, etc.). In O paper clips
general, all scientific measurements that you make should be in metric units,
O electronic or triple beam balances
but you occasionally may obtain measurements in English units or be given
them from another source, so a conversion table is provided in Table 1-1
for your reference. Because you probably have had some prior training with the metric system, the attention to metrics
will be brief here, serving as a review of the basic concepts of the metric system.
In the metric system the basic unit of length is the meter (m), the basic unit of volume is the liter (L), the basic unit
of mass is the gram (g), and the basic unit of temperature is the degree Celsius (°C). The metric system is conveniently
based on units of 10, which simplifies conversions from one metric unit to another. Simply moving a decimal point
to the right or the left is usually all that is needed to convert from one metric unit to another.
Units of area are obtained by squaring the respective metric unit of length (for example, 25 cm2). Units of volume
are obtained either by cubing the respective metric unit of length (for example, 1 cm3) or by measuring the displacement
of the item in a fluid volume (for example, 1 ml).
The metric system was designed around the basic physical properties of water-one of the most abundant com-
pounds on our planet. At sea level, under standard atmospheric pressure (one atmosphere), water boils at 100°C and
freezes at 0°C. One gram (1 g) of water at 4°C occupies one cubic centimeter (1 cm斗, or one milliliter (1 ml), of volume.
Fluid measurements in cubic centimeters (cm3) are commonly abbreviated with the designation “ cc.”
Procedure
1 Obtain a meterstick or metric ruler from your instructor and measure the following items:
Leng·th: Area:
Pencil = cm Student ID card = cm2
2
Staple= 口1日1 Table surface = 日1
Your height = 日1
3 If an electronic balance or a triple beam balance is available, record the mass of the following objects:
Paper clip= mg Coffee cup (empty) = kg
Quarter = g Pencil= g
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763 mm = in. 4.7 L = 37°C = 。F
Ira协 1-1 IConversion Table of Metric Units and Their English Equivalents
Units 。f Length Units of v。 lume Units of Mass Units 。f Temperature
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= 1,000 mm = 0.26 gal. 。。C = 32。F (H20 f .p.)
= 0.001 km = 35 fl . oz. 100。C = 212 。F (H20 b.p.)
1 cm = 0.394 in. 1 ml = 0.035 fl. oz. 1 kg = 1,000 g
= 10 mm = 1 cm3(1 cc) = 2.2 lb.
Materials Needed
The Compound Microscope 口 compound microscope
口 slide of letter 飞”
The compound microscope is a tool that you will use repeatedly in the 口 slide of crossed threads
laboratory to reveal tiny structures and details that cannot be seen with 口 slide of ruler section
the unaided eye. Unfortunately, many students do not refine their microscope (or clear plastic ruler)
skills to their maximum potential and, thus, consistently miss a substantial
portion of the material presented in laboratory exercises that utilize mi-
croscopes. Dozens of hours in front of the microscope will be necessary to polish your technique to the point where
everything you look at is in sharp contrast and clearly focused. Whether you have or have not used a microscope
before, you should read through this section carefully and perform the accompanying exercises. There is something
for everyone to learn here. Your first goal is to familiarize yourself with the mechanical parts of the compound
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Procedure
1 Obtain a compound microscope from the cabinet. Be sure to carry it with both hands while transporting it to
your laboratory bench. Microscopes are expensive, precision instruments that may be damaged easily by dropping
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Procedure
1 Clean the ocular and objective lenses by gently
wiping them with dry lens pape汇 Never use Kimwipes®, paper
towels, or other paper materials
2 Unwrap the cord, plug in the microscope, and turn
on the power switch. If you do not see any light CAUTION to clean microscope lenses!
These materials are abrasive
coming from the illuminato鸟 check to ensure that and may scratch the lenses.
the light intensity slide near the power switch (if
present) is not set to the lowest setting. If that fails,
alert your instructo乌 because you may have a burned-out bulb.
3 Rotate the nosepiece until the low-power objective lens “ clicks ” firmly into place directly over the stage. On
most microscopes the lowest-power lens is either a 4 × or a 10 × lens. The number will be stamped on the side
of the lens. On some microscopes, the lowest-power lens is r eferred to as the “ scanning lens.”
4 Turn the coarse-focus adjustment knob to completely raise the stage until there are only a few milli口1eters between
the stage and the objective lens. On some microscopes, adjustment of the coarse-focus knob moves the entire
microscope body rather than the stage.
5 Place a slide of the letter “ e ” on the stage with the “e” faci吨 upright and centered with the “ e ” directly over the
hole in the stage and surrounded by a circle of light.
6 Adjust the iris dia.p hragm to the midway position, allowi吨 a moderate amount of light to penetrate the slide.
You may open or close the iris diaphragm later as needed to fine-tune the clarity of the image. As a rule of
thumb, the higher the magnification you use, the more light you will need to see an image clearly.
7 Look through the ocular lenses at the letter “ e ” and adjust the distance between the ocular lenses to match the
distance between your pupils. Resist the temptation to close one eye when looking through the microscope!
This is not an effective alternative to using both eyes and will eliminate the advantages of binocular 3-D vision,
as well as contribute to eyestrain and even headaches.
8 While looking through the ocular lenses at the “ e
the ‘‘e ” comes into focus. If you do not see the ‘ Y ’ the slide may be off center. Be sure that the slide is aligned
directly beneath the objective lens and held firmly by the stage clips (if present).
9 R emember-always focus by increasing the distance between the stage and the objective lens. Never focus in
the other direction (decreasing the distance between the stage and the objective lens). This avoids the 且nancial
expense to you of broken slides or damaged objective lenses!
10 Use the fine-focus adjustment knob to finely focus the image in view.
11 Check to see if one of your ocular lenses has an independent focus adjustment. Usually one ocular will have a
series of “ tick marks" on the side with a “。” and “+” and “一” signs . When the “。” is aligned with the indicator
mark on the side of the ocular lens, both ocular lenses h ave equivalent focal distances. If you wear contact lenses
to correct your vision or you have 20/20 vision, you will want the indicator mark on “。” so both oculars have
the same focal plane. If you wear eyeglasses, it is recommended that you remove them when using the microscope,
because this optical adjustment will allow you to correct for differences between your eyes.
12 Rotate the condenser adjustment knob to increase contrast a.n d sharpen the image. The condenser is an often
overlooked but extrem ely valuable component of the microscope that should not be neglected. You will find
its use absolutely necessary to obtain sharp images with certain slides.
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I. I Describe the orientation of the letter “ e ” as it appears through the ocular lenses.
I .2 As you move the slide toward the right of the stage, to which direction does the image of the “ e ” move when
viewed through the microscope?
I.3 As you move the slide away fro1n you on the stage, to which direction does the image move?
Pr。cedure
1 If your microscope is equipped with a 40 × lens, once the image is in focus under medium powe鸟 rotate the
40X objective into place while viewing the slide from the side to check for adequate clearance. If the objective
does not rotate into place without clearing the slide, do not force it. Ask your lab instructor to help you.
2 If you cannot locate the object through the ocular lenses and you find that the 40 × objective is more than a
centimeter away from the slide, you have passed the focal plane of the lens. Therefore, start from the beginning
again with the lowest-power objective and progressively work your way back to the 40 × lens. Be sure not to
lower the stage (or raise the objectives) once you have focused the image and wish to change to the next objective.
3 Your microscope may be equipped with a 100 × lens. This is a special type of lens known as an oil immersion
lens. The working distance is so small between this lens and the slide, and the magnification is so great, that it
is necessary to place a drop of immersion oil between the coverslip of the slide and the 100 × lens to obtain a
clear image. At such high magnification the distortion of light rays caused by light passing through glass, then
air, then into glass again is perceptible and causes a blurred image. Immersion oil has the same optical density
如f agnification
Together, the ocular and objective lenses constitute the magnifying system of your microscope. The initial magnification
of the objective lens provides an image with good detail but is too small for easy examination. The ocular lenses supply
secondary magnification of the initial image so the details are clear enough for normal viewing. The resulting image is
a magnification of a magnified image, and because the properties of magnification are multiplicative, you can easily
calculate the total magnification of a specimen by 1nultiplying together the independent magnification values of each
lens. Remember that the magnification of objectives and oculars is stamped on them.
In Table 1-2, record the values for the total magnification of each of your microscope lens pairs.
Low power ×
Medium power ×
High power ×
Field of View
The circular field that you see when looking through the microscope is described as the field of view. The diameter
of the field of view changes with different magnifications. While still observing the slide of the letter “ e," rotate the
low-, medium-, and high-power objective lenses into place and compare the amount or proportion of the entire letter
that is visible under each one.
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2.1 Under which objective lens is the field of view largest?
2.3 If you did not know what you were looking at already, could you still determine if it was an 飞” using high
power alone? If so, how?
2.4 Which lens (low丁 medium-, or high-power) gives you the largest working distance?
Pr。cedure
1 Obtain a slide of a section of a ruler or a small, clear plastic ruler and place it on the stage.
2 Using low powe鸟 view the ruler through the ocular lenses and esti1nate the size of the field of view by measuring
the diameter in millimeters. You should estimate this value to the nearest 0.1 mm.
3 Repeat this procedure with the medium-power lens in place.
4 Because the ruler (or slide) is too thick to observe using the higher-power lenses, do not attempt 切 measure the
卢eld of view of the 40 × or 100 × lenses. Your instructor can provide the values for the field of view diameters
of those lenses if present on your microscope.
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3.1 The diameter of the field of view of your low-power lens is 日1日1.
3.2 The diameter of the field of view of your medium-power lens is 日1日1.
3.3 Convert these values to micrometers (µm). Remember, there are 1,000 µmin 1 mm.
a. Low-power lens (4 ×)= µm
b. Medium-power lens (10 ×)= µm
c. High-power lens (40 ×)= µm (Obtain this value 斤om your instructor.)
d. Oil immersion lens (100 ×)= µm (Obtain this value 斤om your instructor.)
Depth of Field
The thickness of an image that is acceptably in focus is referred to as the depth of field of a lens. Put another way,
it’s the measure of the distance in front of and beyond the exact focal point of the lens that remains reasonably in
focus. Depth of field varies with the magnification of the objective lens used. You can establish the differences in
depth of field of your objective lenses by viewing a slide containing overlapping objects.
Procedure
1 Obtain a slide with a few strands of overlapping colored threads.
2 First view this slide on low power.
Exploring Zoology: A Laboratory Guide
3 Concentrate on a section where the overlapping of tl川hreads can be seen. Are all three colored threads
reasonably in focus using low power?
4 Now switch to medium power and try to determine which thread is on top of the other two. As you focus
through the image, some threads will be in focus and others will be blurred.
5 Now switch to high power and repeat this procedure. Can all three threads be in focus at the same time with
the high-power lens?
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4.1 Which lens has the greatest depth of field?
Materials Needed
Making a Wet Mount O glass slides
O coverslips
A common way to view living organisms or tissues with the microscope is O pond water culture
to make a wet mount of the specimen. This technique allows you to observe O compound microsζope
movements and properties of living specimens that are impossible to view O plastic droppers
with prepared slides. Figure 1.2 depicts the proper technique for preparing
a wet mount of a biological specimen for viewing with the compound
microscope. In this exercise, you will examine a drop of pond water using your microscope. It is very important to
practice proper microscope technique to reinforce the lessons that you have learned thus far.
Step 1
Step3
Step2
。 M ichael Schenk
Language: English
Kraag's eyes lingered on Jonner's pistol, hanging in the rack with the
heat-guns, and slowly an idea spread through his mind. The heat-gun
range was the same anywhere, but the range of a projectile weapon
should be greater here than on Mars or Earth. Its range should be far
greater than Jonner's rocks.
Kraag took it from the rack and turned it over in his hand, studying it.
He wasn't sure of its principle, but thought it was something on the
order of rocket fuel. It should fire without an atmosphere around it.
There were some figures stamped on the barrel: "COLT 1985,
Cal-.45, MV-1100, Ser-45617298." Kraag puzzled over them. He knew
the first one was the make and year and the last undoubtedly was
the serial number. He deduced that "MV-1100" probably was a figure
showing the relationship between the projectile's mass and velocity.
But it had been a long time since projectile weapons were common.
He called on the memory of a demonstration of the weapon Jonner
had given his companions once on Mars. There was something that
had to be done to prepare it for firing. Holding it in his right hand,
Kraag grasped the barrel with his left. After a moment of hesitant
tugging, he hit the right movement and the whole outer casing of the
barrel slid backward and clicked. It snapped back into position as
Kraag released it, and he remembered.
The gun was primed now. All he had to do was press the trigger and
it would fire. It would automatically prime itself again after firing. It
would fire each time he pressed the trigger now, until it exhausted its
projectiles.
Exultant, he laid it carefully in a contour chair, where it wouldn't slide
out. He put his helmet back on and replaced the hand-hooks of his
spacesuit.
He looked out several ports before he found Jonner. The captain was
not more than 150 feet away, casually lobbing rocks at the sphere.
Kraag picked up Jonner's pistol and made his way down to the
airlock. He emerged and walked around the sphere to the side where
he had located Jonner.
Jonner was moving away now, though he couldn't have known Kraag
was coming out. He was about 300 feet away—too far for a heat-
gun, but certainly within range of the projectile weapon. He seemed
to be headed toward one of the big fuel tanks.
Kraag levelled the pistol toward Jonner and pulled the trigger. To his
astonishment, he was hurtled backward, heels over head.
The kick of a .45 on an asteroid is pretty powerful. Kraag must have
bounced 50 feet backward over the terrain before he slid to rest on
his stomach. But he held on to the pistol—and, since he never had a
chance to release the pressure of his hand-hook on the trigger, it did
not fire again.
When he struggled upright, Jonner was standing at the edge of the
fuel tank, watching him.
"Using my gun now, eh, Kraag?" Jonner said. "You'd better stick to
weapons you know something about."
With that, he disappeared behind the fuel tank.
Kraag got to his feet and advanced confidently. His heat-gun was still
hanging at his belt if he got close enough to Jonner to use it, and he
could fire the projectile weapon at Jonner when Jonner was out of
heat-gun range.
He was learning. One had to point the projectile gun accurately
before firing. It couldn't be swung around and focused while pressing
the trigger, like a heat-gun. He might miss a few times, but he ought
to be able to hit Jonner at least once before the ammunition was
exhausted. Once should be enough.
Heat-gun ready in his left hand, projectile gun in his right, Kraag
circled the fuel tank. Keeping it between them, Jonner had headed
straight for the horizon, running in long, shallow leaps. He was at
least half a mile away.
Kraag pointed the projectile pistol and pulled the trigger. Nothing
happened. Then he realized that he had never released the pressure
of his hand-hook on the trigger after firing the first time. He let up on
it and pressed it down.
And again Kraag was hurled backward, but this time he was smashed
against the fuel tank and rebounded forward, falling on his face. By
the time he reached his feet again, Jonner had vanished over the
horizon.
Cursing softly, Kraag made his way back to the personnel sphere. He
had hoped to get Jonner with that shot. He was very sleepy, and now
he was faced with another night on guard.
He entered the airlock, pushed himself gently upward to catch the
rungs of the metal ladder and turned the wheel of the airlock's inner
door.
Nothing happened. The door did not open.
Fear gripped him like a paralyzing hand. For a moment he thought
Jonner had managed to get to the sphere ahead of him and
somehow had locked him out. But that was impossible. Then he
thought the inner door might be jammed, and he and Jonner locked
out together.
He glanced frantically below him, then broke into relieved laughter.
He had left the outer airlock door open. As a safety measure against
the sphere's accidentally losing its air, neither door would open unless
the other was shut.
And that meant he could lock Jonner out of the sphere simply by
leaving the inner door of the airlock open!
His laugh was full and genuine now as he pulled the outer door
closed.
"Having fun, Kraag?" asked Jonner in his earphones.
"Just looking forward to a good night's sleep, for a change," retorted
Kraag triumphantly. "Prowl around all you want to, Jonner. I can wait
you out, now."
"The airlock, eh? I wondered when your guilty conscience would
settle down and let you remember about that airlock," said Jonner
phlegmatically. "You know, Kraag, I had no idea you wouldn't think
about a simple thing like that, till I looked through the airlock port
last night and saw you huddled up there with a heat-gun. You should
have turned out the light."
Jonner was silent for a few minutes. Then he added:
"I don't think I'd laugh yet, though, Kraag. Remember, you're fighting
with my weapons."
Kraag wasn't sure what he meant by that: whether he was talking
about Kraag's using the projectile pistol or the fact that they were in
space, Jonner's natural element. Kraag himself had been in space 10
years, most of it with Jonner, but before then he had never left Earth.
Jonner had been born and raised on Mars, where a man needed a
suit to go to the next settlement, and he had been on a ship since he
was 15.
As for using the pistol, Kraag could see danger for no one but Jonner.
He had proved, twice, that he could fire it. He was quite sure the old-
fashioned weapon was no more likely to explode than a heat-gun.
The only trouble he foresaw was figuring how to reload it if he used
up all its projectiles before hitting Jonner.
Kraag shrugged and removed his suit. He was hungry, and he was
looking forward to a supper better than Jonner had available in the
concentrated supplies in his spacesuit. Jonner's food and water by
now had dwindled to less than 60 hours' supply, unless he was
weakening himself by going on slim rations.
When Kraag's watch told him it was morning, he opened the ports of
the center deck and let the weak sunlight stream into the sphere. Off
to the east, he saw Jonner digging with a pick from the cargo. Jonner
was far enough away for his legs from the knees down to be hidden
by the extreme curvature of the little planet.
Kraag's first impulse was to go out and take a pot shot at him.
Instead, he switched on the short-wave cooker and prepared some
breakfast. Taking it up to the control room, where he could switch on
the communications system, he opened the eastern port and
watched Jonner. This high, he could see Jonner's feet and the hole he
was digging—and Stein's body.
Jonner had taken Stein's body from the spot outside the sphere
where Kraag had pushed it. He was burying Stein.
Jonner finished his excavation and laid Stein gently to rest in it. He
pushed rocks back in to fill it up, and wrested a boulder that would
have weighed a ton over it for a monument. Then he murmured a
brief prayer over the grave.
Kraag was ashamed and then, unaccountably, angry. But he stood at
the port, drinking his coffee and watching Jonner, and said nothing.
Either with chalk or with some soft rock he had found—Kraag could
not tell which—Jonner wrote something on the big stone that was
Stein's monument. Then he stood up and turned toward the sphere.
"Kraag," he said. "Kraag, are you tuned in?"
"Yes," replied Kraag shortly.
"You have today to surrender. Tonight I'm going to hatch you out of
your comfortable egg."
Kraag switched off the communications system and paced the room,
anger burning slowly inside him. This was ridiculous. He held all the
cards. He had the guns, he had the sphere. Jonner was outside,
weaponless, with a limited supply of food and water. Yet Jonner had
him on the defensive.
How had it happened? How could it happen? Kraag lit a cigarette and
puffed at it slowly, applying his mind coldly to the situation.
He didn't doubt that Jonner would do as he threatened, but he didn't
think it was the recklessness of desperation. More likely, Jonner
deliberately, calculatingly, planned to reduce his own chances for
comfort, in order to bring Kraag down to more even terms with him.
If Jonner broke the hull of the sphere, it could be repaired—by
someone working outside, free from interruption by an enemy. Until it
was repaired, it would mean that Kraag, too, would have to live in a
spacesuit. And Jonner might knock open a hole, or more than one,
big enough to permit him to enter the sphere and attack Kraag in the
darkness.
If only he could surround the sphere with light at night, he could
keep Jonner at a distance. But with the solar mirror gone, the
searchlight, on top of the sphere's other electrical requirements,
would discharge the batteries before the night was half gone.
Kraag knew Jonner's stubbornness, his resourcefulness, his raw
courage. Jonner was the one of them who was really at bay, when
you considered it. Yet Kraag felt that Jonner was closing in on him,
gradually, inexorably.
Facing this, Kraag felt the steel enter his own will. He wasn't a
coward. He had just been expecting this to be too easy. If Jonner
would force him to fight, he would fight. He still had the advantage.
He must abandon the sphere as an asset. Jonner could take that
away from him anyhow. On the other hand, if Jonner took over the
sphere, Kraag could use the same weapon against him. He could
break open the sphere.
So the sphere was no longer a factor. The food and water were no
longer a factor, for food and water went with the sphere. He would
admit Jonner to equality in those supplies—not full equality, for he
could provision himself now more fully than Jonner had been
provisioned two Ceres days earlier. He still might pin Jonner down as
Jonner tried to get to the sphere for more supplies.
Then Kraag's remaining advantage lay in the guns. They should be
enough. If he could get close enough to use a heat-gun, he could
blast Jonner. Jonner's own projectile weapon would keep Jonner out
of rock-throwing range, and sooner or later he would hit Jonner with
it. He couldn't keep on missing; the law of average would give him a
hit sooner or later. And all he needed was just one....
Kraag provisioned his spacesuit and hung all three of the heat-guns
at his belt. In one of the capacious outside pockets he put two spare
flashlights and half a dozen of the extra fuel packets—What was it
Jonner had called them? Magazines, that was it—for Jonner's
projectile pistol. He took that pistol in his right hand and sallied forth
to do battle.
Jonner was nowhere in sight. Kraag shut the outer lock to make it
appear he might be still in the sphere if Jonner happened not to spot
him. He went over to Stein's new grave.
Jonner had written on the stone: REST IN PEACE. R. STEIN
MURDERED BY A. KRAAG. DEC. 12, 2057.
Angrily, Kraag burned the lettering off in a 30-second blast with his
heat-gun that left the face of Stein's gravestone cherry red.
He turned to survey the terrain, and saw Jonner. The captain was
crouched half a mile away, apparently writing more on a flat rock or
on the ground itself.
Jonner was facing him, but his head was down and he hadn't seen
Kraag. If Kraag fired the projectile pistol, he probably would miss and
might warn Jonner with the shot. He was sure of his accuracy with a
heat-gun. Kraag took a heat-gun in his left hand and ran toward
Jonner.
Possibly the vibration of the ground warned Jonner. He looked up,
jumped to his feet and fled. As soon as he could stop and get his feet
planted firmly on the ground, Kraag fired the projectile pistol after
him. He was still shooting low and to one side.
Kraag picked himself up from the ground, where the backlash of the
weapon had knocked him, and went up to the spot where Jonner had
been writing. A mathematical problem had been scratched on the
surface with a sharp rock. Kraag had interrupted Jonner in the middle
of it.
The figures that had been written were: