lab10, Helminthology_082743
lab10, Helminthology_082743
Practical parasitology
Stage :3
Lab:Introduction to helminthology
TEACHING: hiba ahmed
Introduction
• The helminths are worm-like parasites, multicellular eukaryotic
invertebrates
• With tube-like or flattened bodies, bilaterally symmetrical
• The clinically relevant groups are separated according to their
general external shape and the host organ they inhabit.
• There are either hermaphroditic (bisexual) or separate sex
• Helminths classification :
1. Phylum platyhelminths (Trematodes & Cestodes)
2. Phylum nematohelminths (Nematodes)
Flukes (Trematodes)
• Adult flukes are leaf-shaped flatworms.
• Prominent oral and ventral suckers help maintain position in situ.
• Flukes are hermaphroditic EXCEPT for blood flukes, which are
separate sex. The life-cycle includes a snail as intermediate host.
Trematodes Cycle
1. Egg
2. Miracidium
3. Sporocyst
4. Redia
5. Cercaria
6. Metacercaria
7. Adult
Tapewors (Cestodes)
• Adult tapeworms are elongated, segmented, hermaphroditic
flatworms that inhabit the intestinal lumen.
• Larval forms, which are cystic or solid, inhabit extraintestinal
tissues
Cestodes Cycle
1. Egg
2. Metacestode
3. Adult
Roundworms (Nematodes)
• Adult and larval roundworms are separate sex, cylindrical
worms. They inhabit intestinal and extraintestinal sites.
Nematodes Cycle
1. Egg
2. Larvae (L1-L4)
3. Adult
Entry of Pathogenic Helminthes
• Eggs & Larvae are the infective stages
How the Worm Harms the Host ?
• By causing irritation as in Fasciola
• By absorbing the food intended for the host as in Tapeworm
• By sucking blood or lymph as in Hookworm
• By feeding on the tissue of the host as in Ascaris
• By causing mechanical obstruction and pressure as in
Microfiliria
• By causing growth of nodules and tumers and by perforating
vessels as in Wuchereia
• By secreting toxin or harmful substance as anti-coagulants as in
Hookworm
• By transmitting infectious agent as in bacteria, virus, fungi, …
Transmission
• Fecal-oral Transmission
• Transdermal Transmission
• Vector-borne Transmission
• Predator-prey Transmission
Diagnosis
• Microscopic Examination
• Scotch Tape Test T
• Fecal/Stool Examination
• Kato-Katz Technique
• Endoscopy -
• Blood Test :
A. Serology : used to look for antibodies or parasite antigens
antigens
B. Blood Smear : used to look for parasites that are found in the
blood, e.g filariasis can be diagnosed
• Molecular Diagnosis (PCR)
• X-Ray, MRI