CHEM 14CL SP25 FLILL problem set attached

Ngo, C.

b) List the type(s) of electronic transitions for each compound.

*

A .
T
*
B .
I -
I

+ [ *, n +
T
D.-
*,
*
n-

3. Shown below are the structures and colors of an organic indicator in acidic and basic pHs.

Acidic pH (Yellow)
-
Basic pH (Blue)

a) What is the absorption range (nm) in the visible region for the indicator in acidic pH?
-

400 nm + 430 m

b) What is the absorption range (nm) in the visible region for the indicator in basic pH?
-

580n -650nm

c) Provide an explanation of why the indicator has a blue color in a basic pH and has a
yellow color in an acidic pH.

opposite
color
The indicator absorbs the

because the blue indicator is conjugated

more
way
them it has absobs higher i so
the yello ,
so a

b that wavelength
it absobs the solars
completary .

4
CHEM 14CL SP25 Ngo, C.

Important Equations for Review

$ ' = − log ! A = ,-.

!= = 10"#$%
$!
(
2&
!"#$%&#&'( "* +",-"$./ 0 &. 1"#23.' 4 0&(() = 0* 5 9
/= α 2+ + 2&
!"#$%&#&'( "* +",-"$./ 0 &. 1"#23.' 5
0+(() + 0&(() = 0*

hc 1
Ev = = hcv v =
l l

Practice Problems

1. During the Beer-Lambert Law experiments, a student prepared a blank and six standard
solutions in a 1.40 cm cuvette by diluting a 0.50 M CuSO4 stock solution. The absorbance of
each of the standard solutions was then determined at the optimum wavelength. An unknown
solution C was obtained, and its absorbance was measured. The absorbance reading of solution
C was higher than 1.000 (deviated from the Beer's Law calibration plot). Thus, the student
diluted the unknown C with water so that the diluted unknown solution has a concentration
-

-
that is half of the original. The absorbance obtained for this diluted unknown C was 0.76.
Use the equation provided on the Beer's Law calibration plot to determine the
concentration of the original (not the diluted) unknown solution C.

Beer's Law Calibration Plot of Copper (II) Sulfate at 635 nM

1.000 A =
2tC
0.900

0.800
. 76
8 = 1 68067 -

0 0158
0.700
y = 1.6806x - 0.0158 . .

0.600
R² = 0.9973 = 0 46
c
Aborbance

0.500

original
0.400
con).
0.300 2) =

0.200

0 92M CSOn
0.100

0.000 .

0.00 0.10 0.20 0.30 0.40 0.50 0.60

Concentration (M)

2
CHEM 14CL SP25 Ngo, C.

* Shater I
-

* longer 4
n + π

2. Shown below is the list of some natural and synthetic compounds that can absorb UV-Vis
spectrum due to the conjugated pi system: 3π4T
O OH
5π + OH
*
24+ &
O

A: Naphthalene B: 1,3,5-Heptatriene C: Alizarin (from madder root)

HO
OH 972 - π
O

D: Crocetin from saffron

a) Rank the compounds in the order of increasing wavelengths of UV-Vis photons required
to induce electronic excitations.

B A C D
, , ,

3
CHEM 14CL SP25 Ngo, C.

4. Choose the best statement that describes the calibration plot:

Beer's Law Calibration Plot of Chemical Dye X

1.00
0.90
0.80
0.70

Absorbance
0.60
0.50
0.40
0.30
0.20
0.10
0.00
0.000 0.100 0.200 0.300 0.400 0.500 0.600 0.700 0.800 0.900
Concentration (M)

a. A couple of standard solutions are still quite concentrated.

b. The calibration curve is deviated from the expected Beer’s Law response.
c. Too many standard solutions were included in the calibration curve.
d. The wavelength was not fixed during the measurements.

z
e. A proper blank was not included.

5. Choose all possible procedures that describe how a student correctly prepared a diluted
standard solution with the measured absorbance reading of 0.180 from its stock solution. The
measured absorbance for the stock solution is 0.900.
a. Use a volumetric pipet to transfer 5 mL of the stock solution into a 50-mL
-
volumetric flask and dilute the solution with water to the calibration mark

8b. Use volumetric pipets to transfer 10 mL of the stock solution into a 50-mL
volumetric flask and dilute the solution with water to the calibration mark

c. Use volumetric pipets to transfer 20 mL of the stock solution into a 50-mL

volumetric flask and dilute the solution with water to the calibration mark

Dd. Use volumetric pipets to transfer 20 mL of the stock solution into a 100-mL
volumetric flask and dilute the solution with water to the calibration mark

e. Use volumetric pipets to transfer 25 mL of the stock solution into a 100-mL

- volumetric flask and dilute the solution with water to the calibration mark

f. Use volumetric pipets to transfer 50 mL of the stock solution into a 100-mL

- volumetric flask and dilute the solution with water to the calibration mark

0 180 .
= EL
0 9 .
=
EXC
5
CHEM 14CL SP25 Ngo, C.

6. Being familiar with extraction technique from the Caffeine extraction experiment, a student
from CHEM 14CL would like to perform extraction of compound A in their undergrad research
lab. The data table shown below includes the solvents of choice and their corresponding
dielectric constants and solubility values of compound A.

a) Choose the best extraction solvent system consisting of two solvents to extract
compound A from water.
extracting form

most
-

dichloromethane Veter,
must
water belar
experiments
b) Provide sufficient reasoning for your answer in (a)
De
20. .

Dichbronchore is
rompolar,
Roles the greatest a
,
and

is under 20 .
c) Calculate the partition coefficient using solubility values of compound A for the
extraction solvent system chosen in (a).

Solvent Dielectric constant Solubility of compound A

(room temp.) (in molarity)
Acetone 20.7 0.080 M
Diethyl ether 4.3 0.066 M
Dichloromethane
Hexane
9.1
1.9
0.073 M
0.012 M
·
Methanol 32.6 0.098 M
Water 80.4 0.005 M

TM
14.
=
=

Please also check out the supplemental practice problem sets (S-PPS04, S-PPS05 &
S-PPS06) posted on Bruin Learn for extra practice problems.