Prevalence, Etiology and Risk Factors of Mastitis in Cows

Reared Under Dairy Farms and Domestic Environment in

District Nowshera, Pakistan

Scholar’s Name: Salwa Gul

Scholar’s ID # 21840
Program: M.Phil Zoology
Department of Chemical & Life Sciences
Date of Submission: 08/12/2024
Supervisor: Dr. Saira

Qurtuba University of Science & Information Technology

D.I. Khan / Peshawar, Khyber Pakhtunkhwa, Pakistan
 CHAPTER 1

1. Introduction

1.1 Overview

Milk and Dairy Products

A large percentage of the world's population is dependent on milk and dairy products essential nutrients,
making them an important part of the worldwide supply of food. These goods are essential for human well-
being and health since they are abundant in proteins, vitamins, and minerals. With foods like milk, cheese,
yoghurt, and butter being constants in many different cuisines, dairy is an integral part of dietary practices
worldwide.

Growing Global Demand for Dairy

Urbanization, population expansion, and expanding wealth have increased consumption of dairy products,
especially in emerging nations. Dairy has become a vital agricultural item as a result of an ongoing increase in
consumption per person. In order to increase production, the dairy sector worldwide has responded by applying
cutting-edge farming techniques and technologies.
Dairy farming has changed significantly in order to satisfy the rising demand and maintain profitability.
Through improved herd management, better nutrition, and genetic advancement, modern agricultural methods
aim to raise the average milk output per cow. Additionally, commercial farming methods now often involve
greater herd numbers in order to maximize economy of scale and boost productivity.
Dairy farming has seen tremendous production increases as a result of intensification, but there have also been
difficulties. It is essential to strike a balance between environmentally friendly methods and higher productivity.
Discussions on contemporary dairy farming center on topics including animal care, the effects on the
environment, and preserving milk quality. Farmers and researchers work together to find creative answers to
these challenges.

Economic Significance of Dairy Farming

For millions of people worldwide, milk production provides more than simply food; it is their source of
income. By generating revenue & opportunities for employment, it makes a substantial contribution to the rural
economy. The chance for economic expansion rises with herd sizes and milk result. These advantages must be
balanced, though, with the drawbacks of intensification, such as increased resource use and damaging the
environment (De Vliegher et al., 2012).

1.2 Background of Study

Because it's rich in vital elements such proteins, lactose, milk fats, minerals, and vitamins, milk is often
acknowledged as a balanced diet. Pakistan's rural sector depends mainly on livestock farming, giving farming
families steady income and easily liquid assets. With 11.76% of the country's GDP and 56.3% of its total
agricultural value addition, this sector makes an important contribution to Pakistan's agricultural economy
(Government of Pakistan, 2015).
Additionally, Pakistan's international trade is greatly influenced by livestock products; in 2013–2014, milk and
milk-based goods had been imported at a value of Rs.11.78 billion, while meat and related products were
exported at a value of Rs.23.674 billion (Government of Pakistan, 2015).The livestock industry has expanded
remarkably, never seeing a negative growth rate in its history, despite the government's little involvement in its
development. The raising of livestock directly employs about 8 million families in Pakistan (Agriculture
Survey Organization, 2010), creating significant job opportunities for both adults and children.
After the USA & India, Pakistan is globally third-largest producer of milk. Regarding consumption, 26% of
Pakistan's dietary budget comes towards milk and its byproducts (Government of Pakistan, 2015). Dairy
producers, however, have a difficult time getting their products to both urban and rural consumers (Farooq et
al., 2016).
Worldwide, mastitis is a common and economically significant illness in dairy cows. Due to decreased milk
output and treatment costs, it might result in significant losses in the profitability of dairy farms. In certain
situations, particularly when mastitis is severe or persistent, a dairy producer may find it more economical to
cull a cow with the disease rather than invest money on treatment.
Clinical mastitis (CM) and subclinical mastitis (SCM) are the two main types of mastitis. Visual symptoms of
udder inflammation, including redness, swelling, heat, and changes in appearance of milk (such as clots or a
decrease in milk quality), are indicative of clinical mastitis. CM has a wide range of causes, with many
microorganisms being the source of the illness. The way these viruses affect cows and the available treatments
might differ. Bacteria like Streptococcus uberis, Escherichia coli (E. coli), and Staphylococcus aureus are
among the most prevalent pathogens. The kind of pathogen implicated frequently determines the success of
therapy, which might affect the farmer's choice between treatment and culling.
Many farmers still struggle with whether to cure the affected cow, cull it, or do preventative measures, even
with advancements in veterinary treatment. The fact that clinical mastitis milk frequently is not cultured to
pinpoint the specific organism causing the illness is a major obstacle. It is challenging for the farmer to decide
on the best course of action, whether it be supportive care, a particular antibiotic treatment, or preventative
measures meant to lower the risk of recurring, without knowing the specific agent causing the problem.
This diagnostic uncertainty may result in ineffective decision-making, which might raise the financial burden
of mastitis. Thus, reducing the adverse effects of clinical mastitis on dairy farming profitability requires precise
detection of pathogens and focused treatment approaches. Dairy farmers must thus take into account both the
immediate costs and the long-term effects of their mastitis treatment choices (Bar et al., 2007).

Mastitis - Definition, Etymology, and Clinical Manifestations

From the Greek word mastos, meaning "breast," and the suffix -itis, that indicates inflammation, the word
"mastitis" is created. The term "inflammation" itself originates from the Latin word "inflammation" which
means "a kindling, a setting on fire." Given how mastitis manifests clinically, the idea of inflammation as the
word "fire" makes the most sense. The four basic indicators of inflammation are pain, swelling, redness, and
heat. These symptoms contribute to the common clinical manifestations of mastitis by reflecting molecular
events set off by inflammatory agents acting on contaminated tissues.
The increased permeability of blood vessels in the afflicted region is the primary cause of heat, redness, and
swelling in mastitis. The distinctive swelling and heat are caused by the fluid, proteins, and other chemicals
which may seep into the tissues that surround it due to this increased permeability. Increased blood flow to the
irritated region causes redness. Another defining feature of inflammation is pain, which results from immune
cells going to the infection site.
These immune cells—which include neutrophils, macrophages, and lymphocytes—are essential for the body's
defense against infections, but they may also cause the production of various chemical agents that cause nerve
endings to become sensitized, which adds to pain perception. These responses work together as the body's
defense system against the disease.
Clinical mastitis is the result from these processes of inflammation in the breast gland. Visible abnormalities in
the milk and the mammary gland are symptoms of clinical mastitis. The udder may become noticeably bloated,
heated, and uncomfortable to the touch, and the milk could show abnormal characteristics such clots, decreased
yield, or changes in consistency. As the infection spreads and sets off an increased immunological response, the
cow can show symptoms of systemic sickness, such as fever or lack of appetite.However, the clinical
manifestation of mastitis is not always obvious. Subclinical mastitis, in which the infection is not immediately
apparent, happens often. Only diagnostic procedures like bacteriological cultures or somatic cell counts (SCC)
in the milk can identify the inflammation that occurs in this way. The body's immune system includes somatic
cells, mostly leukocytes, which proliferate in reaction to infection.
One of the main markers of subclinical mastitis is their high concentration in milk. Subclinical mastitis affects
milk supply as well as quality even if it doesn't result in visible abnormalities in the milk or udder. For milk
producers, increased somatic cell counts are a major issue since they are linked to lower milk output, changes
in the composition of milk (such as an increase in protein content), and decreased milk shelf life.
The body's attempt to preserve biological homeostasis—the balance required for the healthy operation of
tissues and organs—involves the immune response triggered by both clinical and subclinical mastitis. The goal
of this immune system reaction is to destroy the infection-causing bacteria and return the mammary gland to its
typical state of operation. Even though inflammation serves as a defense mechanism, there may be drawbacks.
The long-term or serious inflammation can lead to scarring, tissue damage, and decreased supply of milk, all of
which have an impact on the health of the cow and the dairy farm's bottom line.
Though the outcome is dependent on the pathogen's type, the extent of the infection, and the cow's immune
system's capacity to deal with the risk, the body's inflammatory response is essential in the resolution of
mastitis. Chronic or recurring mastitis may occur in some situations if the immune system is unable to
adequately treat the infection, which can cause long-term health problems for the cow and continuous
difficulties for the farmer. Thus, for dairy herds to be treated and prevented effectively, it is crucial to
comprehend the biological causes of mastitis, including its clinical and subclinical manifestations (Miles et al.,
2021).

Etiology and Pathogenesis of Mastitis

Clinical mastitis, subclinical mastitis (SCM), and chronic mastitis are the three main types of mastitis, a
condition that is common in dairy herds. The intensity, signs, and effects on milk production of these types
vary. Because it is so minor, subclinical mastitis is the most prevalent of them and presents the biggest obstacle
for dairy farmers. Reduced milk supply without obvious clinical symptoms or milk abnormalities is a common
outcome of SCM. Subclinical mastitis is difficult to diagnose and treat since it often goes unnoticed for longer
due to the absence of obvious signs. Consequently, this type of mastitis contributes to lower milk output and
quality, which has major economic consequences for dairy farms.
An increase in the milk's somatic cell count (SCC) is the main sign of subclinical mastitis. In reaction to
infection, immune cells known as somatic cells go to the mammary gland. Even in the absence of any clinical
symptoms, an increased SCC indicates an inflammatory response to infections. SCM can also result in
modifications to the physical and chemical characteristics of milk, such as a change in the amount of protein
and a shorter shelf life, which can further affect the profitability of farms.
Mastitis has a complicated origin that includes environmental and infectious microbes. Contagious
microorganisms transfer from one cow to a different one during milking, and mostly surviving and multiplying
on the skin and teat infections of sick cows. Conversely, environmental infections do not survive on their teats
and usually come from the cow's surroundings, including water sources, bedding, and waste. Because their
incidence and effects differ according on environmental factors and farm management methods, both kinds of
infections have a part in the development of mastitis.
Several important infections have traditionally been linked to mastitis, and more than 140 different pathogenic
organisms have been discovered as possible causes of the disease. Mastitis has long been known to be primarily
caused by major pathogens, including Streptococcus agalactiae, Staphylococcus aureus, and coliforms,
including Escherichia coli. These microorganisms have a reputation for causing serious infections, especially
when clinical mastitis appears and there are visible symptoms of inflammation.
However, recent research suggests that the main pathogens causing mastitis are now different. Minor pathogens
including coagulase-negative Staphylococcus (CNS) and other bacilli are increasingly being recognized as
being important in the development of mastitis. Previously thought to be less important, current research show
that these small organisms may play a significant role in both subclinical and clinical mastitis instances. These
pathogens frequently vary from one herd to another, which emphasizes how crucial it is to comprehend the
unique microbial environment of a particular dairy farm. Since these small infections may be more challenging
to identify and efficiently treat, the transition from large to small pathogens highlights the necessity of
continuous surveillance and specialised treatments (Pascu et al., 2022).

Clinical Mastitis (CM): A Major Concern in Dairy Cows

Worldwide, dairy cows are susceptible to the common and economically important condition
known as clinical mastitis (CM). Its presence jeopardizes milk production, quality, and dairy
profitability in addition to having an effect on animal health.
Clinical Mastitis Incidence
 The prevalence of CM varies significantly between nations and housing systems. According
to studies, there are between 13 and 40 instances for every 100 cow-years, depending on
factors like:

• Environmental circumstances;

• Farm management techniques;

• Type of housing (dense vs. extensive systems);

• Milking hygiene procedures

Role of Antimicrobial Use in CM Management

• Antimicrobial use on dairy farms is mostly driven by CM, highlighting its significance in managing
herd health. Antimicrobial medication is not indicated in every case of CM, and overuse of antibiotics
can lead to antimicrobial resistance (AMR).
• Mild types of CM or cases involving Escherichia coli frequently resolve without the need of
antibiotics.
• To guarantee efficient management and lower the risks of AMR, appropriate diagnosis and treatment
procedures are crucial.

Recurrence of Mastitis Clinical

The high recurrence rates of CM, especially with certain infections, provide a major management
challenge:
• Escherichia coli: Known to cause environmental mastitis in vulnerable cows, which can develop
quickly and reoccur.
• Streptococcus uberis: Because of its capacity to escape the immune system, this ubiquitous
environmental pathogen is linked to recurring or chronic infections (Jamali et al., 2018).
Clinical and subclinical mastitis are the two universal classifications for mastitis. Along with to the presence of
inflammation-related symptoms in the mammary glands, such as swelling, heat, pain, clinical mastitis is
primarily identified by changes in the milk, such as flakes and clots, as well as systemic symptoms in the
animal, such as fever, rapid heartbeat, appetite loss, dehydration, and depression. The main sign of subclinical
mastitis is absence of obvious change in the milk or udder, but a decrease in milk production, a presence of
bacteria in the secretion, and changes in composition. As a result, only bacterial culture or measuring of a high
somatic cell count (SCC) in milk may be used to clinically detect the milk (Zeryehun et al., 2017).
1. Overview of Chronic Mastitis: Dairy production has major obstacles due to chronic mastitis, a chronic
inflammation of the udder. Chronic mastitis can have long-term effects on the production and well-
being of afflicted cows, in contrast to acute mastitis, which appears quickly.
Possible Results:
Chronic mastitis can have a broad range of outcomes:
Quick Death: Animals suffering from severe systemic illnesses may succumb quickly.

Lack of Udder Functionality: Because of tissue damage or necrosis, which the illness may
cause the udder's afflicted quarters to become partially or completely absent.
Chronic Intramammary Infection: Prolonged infections have the potential to harm the
mammary gland permanently and result in abscesses.
Elevated Somatic Cell Count (SCC): A sign of mastitis, elevated SCC denotes decreased milk
quality and inflammation.
Full Recovery: Although less frequent, recovery is possible with the right care and supervision
(Grant et al., 2016).

Different pathogens that differ from one community to another cause mastitis. The most prevalent pathogens
are coliforms (Escherichia coli and Klebsiella pneumoniae), streptococci (Streptococcus agalactiae,
Streptococcus dysgalactiae, Streptococcus uberis, and Streptococcus bovis), and staphylococci (Staphylococcus
aureus and Staphylococcus epidermidis). Yeast, Nocardia, Mycoplasma, and Pseudomonas species are less
common culprits. According to studies, coagulase-negative staphylococcus (CNS) is a common udder infection
pathogen, and buffalo mastitis cases are frequently caused by staphylococci, streptococci, E. coli, and
pseudomonas. The most common pathogen in instances of cow mastitis is Staphylococcus aureus.
There are two types of mastitis pathogens: infectious and environmental. The majority of clinical cases are
caused by infectious microorganisms, such as S. aureus and Streptococcus agalactiae, which are transmitted
during milking by flies, sharing cloth towels, or contaminated hands. However, coliforms like E. coli and
Klebsiella, as well as environmental infections like Streptococcus uberis and Streptococcus dysgalactiae, are
spread from one milking to the next. Unsanitary conditions are frequently the cause of coliform infections, and
sawdust containing bark or dirt is frequently linked to Klebsiella. Swollen udders or quarters, watery or
irregular milk, and decreased appetite are symptoms of these illnesses (Sharif et al., 2009).
One of the most common diseases affecting dairy cows is mastitis, which is an infection of the mammary
gland. Because of the enormous financial losses, it causes, the harm it does to the health and wellbeing of
animals, and the negative impacts it has on the amount and quality of milk, it is regarded as the most expensive
and destructive illness in the dairy business. The main dairy animals afflicted by this illness in Pakistan are
cattle and buffaloes.Mastitis induces alterations in cell permeability, decreases milk synthesis, and drastically
modifies the chemical makeup of milk. The majority of mastitis instances (70%) are caused by bacterial
infections, with the remaining 30% coming from non-infectious causes such mechanical damage to the gland
and physical trauma.
Twenty pathogenic bacteria are most frequently implicated in cow mastitis, while over 135 bacterial species
have been linked to the condition. The most prevalent mastitis-causing bacterial pathogens include
Staphylococcus aureus, Escherichia coli, coagulase-negative staphylococci (CNS), Streptococcus dysgalactiae,
Streptococcus uberis, and Streptococcus agalactiae (Ali et al., 2021).

Prevalence and Classification of Mastitis

Depending on its cause, mastitis, an infection of the mammary gland, can be categorised as either infectious or
environmental. Microorganisms such as Streptococcus uberis, Klebsiella pneumoniae, and E. coli that are
present in the animal's bedding, faeces, or urine can cause environmental mastitis. On the other hand,
contagious mastitis, which is mostly brought on by bacteria such as Streptococcus uberis, Streptococcus
agalactiae, and Staphylococcus aureus, is transmitted from diseased cows during milking.
Another way to categorize mastitis is as clinical or subclinical. While preclinical mastitis has no outward
symptoms but results in decreased milk supply and elevated somatic cell counts (SCC), clinical mastitis
exhibits outward symptoms such redness, swelling, and abnormal milk. Subclinical mastitis is more prevalent,
more difficult to identify, and more chronic.
Major Pathogens Causing Mastitis

Pathogens that cause mastitis can be categorized as severe or minor depending on their severity and frequency.
E. Coli, Streptococcus uberis, Streptococcus agalactiae, Staphylococcus aureus, Streptococcus dysgalactiae,
and Klebsiella pneumoniae are important pathogens.
1.E. coli, or Escherichia coli

• Type: an environmental pathogen.

• Prevalence: Typical during dry spells and the early stages of breastfeeding.
• Pathogenicity: Endotoxins harm breast tissue, resulting in moderate to severe mastitis.
• Factors: Vaccination, energy balance, vitamin status, breastfeeding stage, and infection level all affect
severity.
• Treatment: Regular milking aids in minor infections, but antibiotics are required for more serious ones.
• Control: To avoid infections during vital times, dry cow management must be done well.

2. Klebsiella pneumoniae
• Type: Pathogen in the environment
• Prevalence: Present in soil, water, and bedding materials.
• Pathogenicity: Endotoxins harm tissue and cause mastitis of various intensities, sometimes fatal.
• Impact: Significant reductions in milk output.
• Control: Better environmental and bedding hygiene.
3. Uberis Streptococcus
• Type: Environmental; potentially communicable.
• Prevalence: On cow corpses, in pastures, and in bedding.
• Pathogenicity: During the dry season, it causes mastitis, which develops into clinical manifestations during
breastfeeding.
• Treatment: Although reinfections are frequent, antibiotic treatment is successful.
• Control: Hygiene, disinfection, and appropriate bedding handling.
4. The agalactiae Streptococcus
• Type: infectious disease
• Prevalence: Linked to unsanitary methods of milking.
The ability to spread during milking and to stay subclinically in udders is known as pathogenicity.
• Treatment: Effective but expensive, blitz therapy, which treats the entire herd, works well with antibiotics.
• Control: New animal quarantine and good cleanliness.

5. Staphylococcus aureus
• Type: infectious disease
• Prevalence: Serious mastitis issue, frequently chronic and subclinical.
• Pathogenicity: Low antibiotic cure rate because to udder fibrosis and abscess development.
• Impact: Long-term reductions in milk production; in chronic situations, culling may be required.
• Control: Preventing infections by keeping animals clean and removing afflicted ones as soon as possible.
6. The dysgalactiae Streptococcus
• Type: contagious and environmental
• Prevalence: Often confused with other Streptococcus species, it is found in bedding, body parts, and the
environment.
• Pathogenicity: In the summer, it can be spread by vectors (flies) and causes acute inflammation.
• Control: Good environmental management and cleanliness.
Measures for General Control
• Better hygiene and management of the herd.
• Proper teat disinfection and dry cow treatment.
• Timely removal of high-risk bedding items and treatment of infections.
• Programs for vaccination against certain infections, such E. coli.

Effects of Mastitis on Dairy Production

Both clinical and subclinical mastitis cause large losses in milk output, with economic consequences
that go beyond the illness itself. Important conclusions include:
1. Losses in Milk Production:
o Even after recovery, subclinical mastitis causes ongoing decreases in milk output. Although they may
not return production to normal, antibiotic treatments may stop the development of clinical forms.
Significant decreases in milk output are caused by clinical mastitis; losses can reach 20% for infections
caused by Streptococcus dysgalactiae and 50% for infections caused by Escherichia coli.
2. Economic Expenses:
o The estimated expenses per case are GBP 131 (EUR 198), USD 179 (EUR 162), USD 155 (EUR
140), and USD 148 (EUR 134), depending on the area and severity.
o These expenses cover labour, veterinary care, treatment, wasted milk, losses in production, feed for
wasted milk, and illness-related

Milk Composition Changes:

o There are notable decreases in fat, casein, and lactose in infected milk.
o Minor pathogen-induced subclinical mastitis can considerably raise the somatic cell count (SCC) but may not
have a major impact on protein or fat contents.
3. Throwaway Milk:
o Because it still includes feeding expenses, milk from clinically diseased cows must be rejected, resulting in
greater losses than decreased output.
o By lowering the expense of milk replacer, feeding calves leftover milk helps minimise losses.
4. Indirect Losses: o There are notable average milk losses of 375 kg per lactation and a shorter lactation
duration (by around 57 days).
o Culling, higher mortality, and long-term output decreases all add to budgetary pressures.
5. Classification of Costs: o Preventive Costs: Materials for programs aimed at prevention and control.
o Failure Costs: Resources for rehabilitation and treatment, such as(Cobirka et al., 2020).

1.3 Problem Statement

Mastitis, an inflammation of the mammary glands, is one of the most significant and economically detrimental
diseases in dairy farming worldwide. This condition affects milk production, quality, and, in severe cases, the
overall health and longevity of the affected cows. Mastitis not only causes physical discomfort and suffering in
animals but also imposes financial losses on farmers due to reduced milk yield, increased veterinary expenses,
and premature culling of cows. In Pakistan, dairy farming is a vital sector contributing significantly to the
livelihoods of rural populations and the economy. However, mastitis continues to pose a substantial challenge
in maintaining high milk quality and ensuring the profitability of dairy farming systems.

The prevalence and severity of mastitis are influenced by various factors, including environmental conditions,
milking practices, hygiene standards, and the overall management of dairy cows. In District Nowshera,
Pakistan, dairy cows are reared under two distinct management systems: organized dairy farms and small-scale
domestic household settings. Organized farms typically adhere to standardized practices such as routine health
monitoring, structured feeding regimens, and controlled environments. In contrast, cows in domestic settings
are often kept in less formalized systems, with varying levels of hygiene and inconsistent health monitoring
practices. These differences in management systems play a critical role in the occurrence, sources, and risk
factors associated with mastitis.

Despite the high economic and health implications of mastitis, limited research has been conducted to compare
its prevalence and contributing factors in different rearing environments within District Nowshera. Most
existing studies focus on a single management system or broader regional assessments, leaving a significant
gap in localized data that addresses the specific dynamics of mastitis within varied management contexts.
Bridging this knowledge gap is essential for devising tailored interventions to effectively manage and prevent
mastitis in the region.
The primary purpose of this research is to establish baseline data on the prevalence, etiology, and risk factors
associated with mastitis in dairy cows reared under two different management systems—dairy farms and
domestic household environments—in District Nowshera, Pakistan. The study aims to identify significant
differences in the occurrence of mastitis, its sources, and contributing factors between these two settings. This
comparative approach will provide valuable insights into the underlying causes of mastitis and inform effective
prevention and control strategies tailored to each rearing environment.

This research will employ a systematic methodology, including field surveys, clinical examinations,
microbiological analyses, and statistical evaluations. By examining the interplay of management practices,
hygiene conditions, and environmental factors, the study seeks to uncover critical points of intervention to
reduce mastitis prevalence.
Understanding the prevalence and risk factors of mastitis in both farm and domestic settings is crucial for
improving dairy cow health, enhancing milk production, and ensuring sustainable dairy farming practices. This
study will provide foundational data that can guide policymakers, veterinarians, and farmers in implementing
targeted strategies to combat mastitis effectively.

For dairy farms, insights from this research may lead to recommendations for adopting advanced health
monitoring systems, improving milking hygiene, and optimizing environmental conditions. For domestic
settings, the findings could help promote cost-effective and practical measures, such as improved sanitation,
basic veterinary care, and education on best milking practices.

Additionally, this research has broader implications for public health and the dairy industry. Milk from mastitis
cows often exhibits reduced quality, with lower nutritional value and potential contamination risks. By
addressing mastitis, this study contributes to ensuring the production of safe and high-quality dairy products for
consumers.

1.4 Aims and Objectives

1. To assess the prevalence of mastitis in cows kept under dairy farms and domestic environment.
2. To identify the bacterial species which causes mastitis in cows and risk factors contributing to mastitis.

1.5 Significance & Scope of Study

Significance of the Study
In dairy farming, mastitis is a common issue that has a significant impact on milk supply, quality, and
profitability. Effective management of mastitis can have major economical and public health ramifications in
Pakistan, where the dairy industry is a vital component of the rural economy. The frequency, causes, and risk
factors of mastitis in dairy cows raised in two different environments—organized dairy farms and residential
settings—are better understood thanks to this study.
This study's ability to close the information gap on the etiological agents and local risk factors linked to mastitis
in District Nowshera is one of its main significances. The study's focus on this area will yield much-needed
data that captures the distinct environmental, managerial, and cultural practices of home and farm systems.
These kinds of insights are essential for developing practical, economical, and locally appropriate preventative
and control measures.
The results of this study will help policymakers and dairy producers comprehend how various management
techniques affect the prevalence of mastitis. On organized farms, where infrastructure and resources could be
available, suggestions might center on improving current procedures including frequent health checks,
biosecurity precautions, and milking cleanliness. In contrast, the study will provide workable, affordable
solutions that can dramatically lower the incidence of mastitis in domestic home settings, where there are
frequently budgetary limitations and restricted veterinarian access.
Additionally, this study fills a significant public health vacuum. Milk quality can be harmed by mastitis, which
can result in the presence of dangerous bacteria or leftovers. This study indirectly promotes the development of
safer dairy products by identifying the causes of mastitis, protecting the health of consumers in the process. The
findings can help regulatory agencies create guidelines for milk safety and raise public awareness of the
dangers of milk contaminated with mastitis.
The study's ramifications also affect farmers' financial security. Reduced milk production, increased veterinary
expenses, and, in extreme situations, the early culling of cows are all consequences of mastitis. Since small-
scale farmers make up the bulk of dairy producers in District Nowshera, they are disproportionately impacted
by these financial restrictions. The study intends to lessen these financial strains and support the sustainability
of smallholder dairy production by outlining practical preventative measures.
Finally, this work will provide a basis for further research. Further research into enhanced control techniques,
genetic resistance to mastitis, and the socioeconomic effects of the illness is made possible by the establishment
of baseline data on the prevalence, aetiology, and risk factors of mastitis in the area.
Scope of the Study
This study's focus on mastitis in dairy cows within the geographical setting of District Nowshera, Pakistan,
defines its scope. The two main management methods included in the study are household-based residential
settings and organized dairy farms. The research aims to capture the wide variety of variables impacting the
occurrence and development of mastitis by looking at these different habitats.
Geographic Range
Because of its significance in dairy production and its representation of both organized and home raising
systems, District Nowshera was selected as the research location. The district is a perfect case study for
comprehending the dynamics of mastitis in rural Pakistan because of its environment, socioeconomic
circumstances, and farming customs. Even if the results are unique to Nowshera, they could potentially apply
to other areas with comparable circumstances.

Scope of Biology and Etiology

The clinical and subclinical manifestations of mastitis in dairy cows are the main topics of this investigation.
While subclinical mastitis frequently remains undetected without diagnostic testing, clinical mastitis is
distinguished by outward signs such swelling, redness, and irregular milk. To find the causing pathogens, such
as Staphylococcus aureus, Escherichia coli, and other common bacteria, the study uses microbiological studies.
Developing focused treatment and preventative plans requires an understanding of the etiological factors.

Comparative Scope

A major component of this research is the comparative analysis of mastitis prevalence, sources, and risk factors
between dairy farms and household settings. This comparison will shed light on how management practices,
hygiene standards, feeding regimes, and environmental factors differ between the two systems and how these
differences influence mastitis dynamics.

Economic and Quality Implications

The study examines the impact of mastitis on milk yield, quality, and overall economic losses. By evaluating
these parameters, the research aims to quantify the extent of the problem and provide actionable insights for
farmers to minimize losses and improve productivity.
Temporal Scope

The study is designed as a cross-sectional investigation conducted over a defined period. This temporal
limitation provides a snapshot of mastitis prevalence and risk factors but does not capture seasonal variations or
long-term trends. Future research could build on this foundation by incorporating longitudinal studies.

Methodological Scope

This research employs a combination of field surveys, clinical examinations, and laboratory analyses. Field
surveys involve data collection on management practices, hygiene conditions, and farmer knowledge about
mastitis. Clinical examinations focus on detecting visible and subclinical cases, while microbiological analyses
identify the causative agents. Statistical methods, including comparative tests, will be used to analyze and
interpret the data.

Limitations and Delimitations

While the study covers significant ground, certain limitations exist. For instance, the reliance on farmer-
reported data may introduce biases related to recall or awareness. Additionally, the cross-sectional nature of the
study limits its ability to capture seasonal or long-term trends. Despite these limitations, the study’s design
ensures that the findings will be robust, reliable, and valuable for the targeted objectives.

By delineating its significance and scope, this research positions itself as a critical contribution to
understanding and managing mastitis in dairy cows in District Nowshera. The results will inform practical
interventions, enhance dairy sector sustainability, and serve as a stepping stone for future investigations.

References

Ali, T., Kamran, N., Raziq, A., Wazir, I., Ullah, R., Shah, P., ... & Liu, G. (2021). Prevalence of mastitis
pathogens and antimicrobial susceptibility of isolates from cattle and buffaloes in Northwest of
Pakistan. Frontiers in Veterinary Science, 8, 746755.

Bar, D., Gröhn, Y. T., Bennett, G., González, R. N., Hertl, J. A., Schulte, H. F., ... & Schukken, Y. H.
(2007). Effect of repeated episodes of generic clinical mastitis on milk yield in dairy cows. Journal
of Dairy Science, 90(10), 4643-4653.
Cobirka, M., Tancin, V., & Slama, P. (2020). Epidemiology and classification of
mastitis. Animals, 10(12), 2212.

De Vliegher, S., Fox, L. K., Piepers, S., McDougall, S., & Barkema, H. W. (2012). Invited review: Mastitis
in dairy heifers: Nature of the disease, potential impact, prevention, and control. Journal of dairy
science, 95(3), 1025-1040.
Farooq, U. (2016). Milk supplies in Pakistan: issues and challenges facing the dairy economy. Pakistan
Agricultural.
Grant, C., Smith, E. M., & Green, L. E. (2016). A longitudinal study of factors associated with acute and
chronic mastitis and their impact on lamb growth rate in 10 suckler sheep flocks in Great
Britain. Preventive veterinary medicine, 127, 27-36.
Jamali, H., Barkema, H. W., Jacques, M., Lavallée-Bourget, E. M., Malouin, F., Saini, V., ... & Dufour, S.
(2018). Invited review: Incidence, risk factors, and effects of clinical mastitis recurrence in dairy
cows. Journal of dairy science, 101(6), 4729-4746.
Miles, A. M., & Huson, H. J. (2021). Graduate student literature review: understanding the genetic
mechanisms underlying mastitis. Journal of dairy science, 104(1), 1183-1191
Pascu, C., Herman, V., Iancu, I., & Costinar, L. (2022). Etiology of mastitis and antimicrobial resistance in
dairy cattle farms in the western part of Romania. Antibiotics, 11(1), 57.
Sharif, A. A. M. I. R., Umer, M. U. H. A. M. M. A. D., & Muhammad, G. H. U. L. A. M. (2009). Mastitis
control in dairy production. J. Agric. Soc. Sci, 5(3), 102-105.
Zeryehun, T., & Abera, G. (2017). Prevalence and bacterial isolates of mastitis in dairy farms in selected
districts of Eastern Harrarghe zone, Eastern Ethiopia. Journal of veterinary medicine, 2017(1),
6498618.