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SubMandibularBloodCollectionMice_1 (1)

The document outlines the IACUC standard procedure for submandibular blood collection in mice, emphasizing its advantages of maximum sample volume with minimal trauma. The procedure requires anesthesia, trained personnel, and specific supplies, detailing steps for safe and effective blood collection. Potential adverse effects include hemorrhage and hematoma formation, necessitating careful monitoring during the process.

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3 views

SubMandibularBloodCollectionMice_1 (1)

The document outlines the IACUC standard procedure for submandibular blood collection in mice, emphasizing its advantages of maximum sample volume with minimal trauma. The procedure requires anesthesia, trained personnel, and specific supplies, detailing steps for safe and effective blood collection. Potential adverse effects include hemorrhage and hematoma formation, necessitating careful monitoring during the process.

Uploaded by

drylok360
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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Submandibular Blood

Collection in Mice
IACUC Standard Procedure
Effective Date: May 2016

Description of procedure:

Blood collection from the submandibular vein is the preferred blood collection technique for mice. This
method allows for maximum allowable sample volume with minimal trauma to the animal. Multiple
samples can be taken daily by alternating sides. This technique must be performed on anesthetized
animals. Only trained personnel may perform this procedure.

This technique may yield a large sample volume so will not be appropriate for frequent small blood
volume collection. For information regarding maximum blood collection volume please refer to the UCSF
Blood Collection Guidelines.

Supplies necessary:
• Isoflurane anesthesia system
• 25 gauge needles or Lancet
• Gauze sponges
• Blood collection tube

Procedure:
1. Anesthetize the mouse with isoflurane in an induction chamber.
2. Remove the mouse from the induction chamber and restrain the mouse with the non-dominant
hand by grasping the loose skin over the shoulders and behind the ears; the skin should be taut
over the mandible
3. Puncture the vein with a 25 gauge needle or lancet slightly behind the mandible. A swift lancing
motion is used to puncture the vessel (see Image 3 below). Only the tip of the needle should
enter the vessel to a shallow depth of 1-2 mm. Blood will flow immediately.
4. Collect sample with a pipette or other collection tube.
5. When the sample has been collected apply gentle pressure to the blood collection site with a
gauze sponge until bleeding has stopped.
6. The animal may be returned to their home cage once they have fully recovered from the
anesthesia.

To determine the appropriate location of the puncture site, locate the hairless spot along the jaw line
(Image 1), measure approximately 0.5 cm superior and lateral towards the shoulder (Image 2).

Image 1 Image 2
Image 3: Vasculature and demonstration of blood collection.

Agents: This procedure requires gas anesthesia. All agents administered to animals should be listed in the
"Agents" section of RIO.

Adverse effects to be considered: Hemorrhage from the ear canal or nasal cavity, hematoma formation

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