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BBT-603

This document is an examination paper for the B. Tech (Biotechnology) course at D. Y. Patil Deemed to be University, focusing on Bioprocess Technology. It includes various questions on topics such as solid substrate fermentation, sterilization processes, fermentation economics, and effluent treatment. The exam consists of multiple sections with compulsory questions, diagrams, and comparisons required for comprehensive responses.

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0 views2 pages

BBT-603

This document is an examination paper for the B. Tech (Biotechnology) course at D. Y. Patil Deemed to be University, focusing on Bioprocess Technology. It includes various questions on topics such as solid substrate fermentation, sterilization processes, fermentation economics, and effluent treatment. The exam consists of multiple sections with compulsory questions, diagrams, and comparisons required for comprehensive responses.

Uploaded by

prathamrex14
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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D. Y.

PATIL DEEMED TO BE UNIVERSITY, NAVI MUMBAI


2024.01
B. Tech (Biotechnology)
BBT-603: Bioprocess Technology
Additional Requirements:NIL
Duration: 2.30 Hrs Max Marks: 60
Note: 1. All questions are Compulsory.
2. All question carry equal marks.
3. Draw diagrams, illustrations, tasks etc. wherever necessary.

Q.1 Answer the following (Any Four): [12]


a) Define solid substrate fermentation and give any two characteristics of the same.
b) Justify: filter sterilization is more suitable for industrial media sterilization.
c) Discuss the physical characteristics of the effluent that are studied before the actual
treatment.
d) How does scale-down studies contribute to process understanding and optimization in
bioprocessing?
e) What are the common types of steam traps used in bioreactor systems?
f) Elaborate on the role of media cost in maintaining fermentation economics.

Q.2 Attempt any one of the following from each sub-questions


a) Diagrammatically describe the basic principle of operation of an airlift reactor and [6]
mention its primary application areas.
OR
b) Compare SSF with submerged fermentation on the following points: i) Product Yield [6]
and Quality ii) Microbial Growth and Metabolism.

c) With a flowsheet explain the working of the photobioreactor. [4]


OR
d) Explain the key steps involved in the upstream processing of fermentation. [4]

e) List two advantages of using fluidized bed reactors compared to fixed-bed reactors in
[2]
chemical processes.
OR
f) Discuss the role of the feed pump and peristaltic pump. [2]

Q.3 Attempt any one of the following from each sub-questions


a) Explain why sterilization of growth media is crucial and how the process of batch
[6]
sterilization is carried out.
OR
b) Discuss the microbial kinetics for continuous fermentation. [6]

c) Explain the role of sulfur and phosphorus in microbial nutrition. [4]


OR
d) Discuss the principle behind the use of Mac Conkey’s medium in microbiology. [4]

e) What is a synthetic medium? Give two examples. [2]


OR
f) Elaborate on the factors that influence heat sterilization. [2]

P.T.O.
Q.4 Attempt any one of the following from each sub-question
a) Explain the different mechanical processes used in the effluent treatment. [6]
OR
b) Discuss the dissolved oxygen control in fermentation process. [6]

c) Explain temperature control in fermentation process. [4]


OR
d) What are the main contaminants found in the industrial wastewater? [4]

e) Explain the controlling mechanism used for pressure control in fermentation. [2]
OR
f) What is chemical oxygen demand? [2]

Q.5 Attempt any one of the following from each sub-question


a) What is fermentation economics? Elaborate on the role of instrumentation and [6]
downstream processing in deciding fermentation economics.
OR
b) Which aspects are mainly studied while scaling down a fermentation process? [6]

c) Justify: One must pay keen attention to the mass transfer of oxygen between big and
[4]
small fermenters in scale-down studies.
OR
d) Elaborate on the role of oxygen uptake rate in fermentation. [4]

e) Why is there a need to focus on inoculum development during scale-up? [2]


OR
f) Give any two reasons for scaling up an industrial process. [2]

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