NAME: BASSEY GOODNESS EMMANUEL

DEPARTMENT: MEDICAL REHABILITATION

FACULTY: HEALTH SCIENCES AND TECHNOLOGY

COURSE CODE: BIO 151

REGISTRATION NUMBER: 202440022289HA

DATE: 5TH APRIL, 2025

HISTORY AND CLASSIFICATION OF VIRUSES

Introduction

A virus is a microscopic infectious agent that is non-cellular, consisting of genetic material

(either DNA or RNA) enclosed within a protein coat called a capsid, and in some cases, an
additional lipid envelope. Viruses are obligate intracellular parasites, meaning they cannot
reproduce or carry out metabolic processes outside a host cell. They infect all forms of life,
including animals, plants, fungi, bacteria (bacteriophages), and archaea.
Viruses are microscopic infectious agents that can replicate only inside living cells. Despite their
simplicity, viruses have had an enormous impact on the biological world, influencing the
evolution of species and shaping medical science. They are responsible for a range of human,
animal, and plant diseases, from the common cold to pandemics like COVID-19. This paper
explores the history of virus discovery, their structural and genetic diversity, mechanisms of
replication, interaction with hosts, and the systems developed for their classification.
Understanding these aspects is vital for combating viral diseases and harnessing viruses for
beneficial applications in medicine and biotechnology.

Characteristics of Viruses

1. Acellular Structure:
Viruses are not made up of cells and lack the cellular machinery necessary for metabolism and
replication.

2. Genetic Material:
A virus contains either DNA or RNA (never both), which may be single-stranded or
double-stranded, linear or circular, and segmented or non-segmented.

3. Protein Coat (Capsid):

The capsid protects the viral genome and facilitates attachment to and penetration into host cells.
It is made up of protein subunits called capsomeres.

4. Envelope (in some viruses):

Some viruses have a lipid envelope derived from the host cell membrane. This envelope may
contain viral glycoproteins crucial for recognizing and entering host cells.

5. Lack of Metabolic Machinery:

Viruses do not possess ribosomes, enzymes for energy production, or other organelles; hence,
they rely entirely on the host cell’s machinery for replication and protein synthesis.

6. Host Specificity:
Viruses are often highly specific to the host species and even to certain cell types within the host.

7. Replication Through Assembly:

Viruses replicate by assembling newly synthesized viral components within the host cell, not by
cell division.

History of Viruses

Early Concepts of Disease

Before the scientific discovery of microorganisms, diseases were attributed to supernatural forces
or environmental miasmas. The germ theory of disease, developed in the 19th century by
scientists like Louis Pasteur and Robert Koch, laid the groundwork for identifying pathogens,
including viruses.

Discovery of Viruses
The discovery of viruses began in 1892 when Dmitri Ivanovsky observed that sap from diseased
tobacco plants remained infectious after being filtered to remove bacteria. In 1898, Martinus
Beijerinck confirmed this and coined the term "virus," meaning poison. He proposed that these
infectious agents were not bacterial but a new form of pathogen.

20th Century Virology Breakthroughs

In the early 20th century, virology gained momentum. In 1915 and 1917, bacteriophages were
independently discovered by Frederick Twort and Félix d'Herelle. These viruses infect bacteria
and were critical in early molecular biology research. The invention of the electron microscope
in the 1930s allowed scientists to visualize viruses for the first time, confirming their particulate
nature.

Discovery of Specific Virus Families

With new techniques, researchers identified major virus families such as Herpesviridae,
Orthomyxoviridae, and Retroviridae. The development of cell culture methods in the 1940s
enabled the isolation and propagation of viruses, facilitating vaccine development.

Advances Through Molecular Biology

The 1970s and 1980s saw a revolution in virology with the emergence of molecular biology
techniques. Sequencing viral genomes and understanding their replication mechanisms became
possible. This period also witnessed the identification of oncogenic viruses and the Human
Immunodeficiency Virus (HIV).

Important Historical Case Studies

Smallpox Eradication

Smallpox, caused by the Variola virus, was a devastating disease for centuries. The global
vaccination campaign led by the World Health Organization resulted in the eradication of
smallpox in 1980, marking a monumental achievement in virology and public health.

Polio Outbreaks and Vaccines

Poliovirus caused widespread outbreaks during the 20th century, particularly affecting children.
The development of inactivated and oral polio vaccines by Jonas Salk and Albert Sabin
drastically reduced incidence rates worldwide.
HIV/AIDS Crisis

Discovered in the early 1980s, HIV causes AIDS and has led to millions of deaths globally.
Research into HIV led to major advances in understanding retroviruses and the development of
antiretroviral therapies.

SARS, MERS, and COVID-19

Coronaviruses have caused several significant outbreaks: SARS in 2003, MERS in 2012, and
COVID-19 in 2019. SARS-CoV-2, the virus behind COVID-19, has had profound social and
economic impacts, emphasizing the importance of virology in pandemic preparedness.

Virus Structure and Life Cycle

Structural Diversity

Viruses vary in shape and size. They may be helical, icosahedral, or complex. Most viruses
consist of a nucleic acid core, a protein capsid, and sometimes a lipid envelope derived from the
host cell membrane.

Replication Mechanisms

Viruses replicate by entering a host cell and hijacking its machinery. The lytic cycle involves
rapid replication and host cell lysis, while the lysogenic cycle involves viral genome integration
and latency.

Genetic Material in Viruses

DNA Viruses

DNA viruses can have single-stranded (ssDNA) or double-stranded (dsDNA) genomes.

Examples include Herpesviridae (dsDNA) and Parvoviridae (ssDNA).

RNA Viruses
RNA viruses are categorized as positive-sense (+ssRNA), negative-sense (-ssRNA), or
double-stranded (dsRNA). Examples include Coronaviridae (+ssRNA) and Orthomyxoviridae
(-ssRNA).

Retroviruses

Retroviruses, such as HIV, possess ssRNA genomes and reverse transcriptase enzymes to
synthesize DNA from RNA, which integrates into the host genome.

Virus-Host Interactions

Entry and Replication

Viruses enter host cells via receptor-mediated endocytosis or membrane fusion. After uncoating,
the viral genome is replicated and proteins are synthesized.

Immune Evasion

Viruses have evolved strategies to evade host immunity, such as antigenic variation, inhibition of
interferons, and latency.

Effects on Host Cells

Some viruses cause cytopathic effects, while others integrate into the host genome, potentially
leading to oncogenesis.

Classification of Viruses

a.ICTV System

The International Committee on Taxonomy of Viruses (ICTV) classifies viruses based on

morphology, genome type, and replication mechanism. Classification includes realms, kingdoms,
orders, families, genera, and species.

b. Baltimore Classification

David Baltimore proposed a system classifying viruses into seven groups based on how they
generate mRNA:
Group I: dsDNA viruses

Group II: ssDNA viruses

Group III: dsRNA viruses

Group IV: +ssRNA viruses

Group V: -ssRNA viruses

Group VI: ssRNA-RT viruses

Group VII: dsDNA-RT viruses

c. Comparative Analysis

While ICTV focuses on taxonomy, Baltimore's system emphasizes replication strategies. Both
are complementary and widely used in virology.

d.Classification by host
Viruses can be classified based on the type of organism they infect:

Animal Viruses: These are viruses that infect animals and humans. Examples include: rabies,
avian influenza (bird flu), foot and mouth disease.

Plant Viruses: These are viruses that infect plants. Examples include: Tobacco Mosaic Virus.

Bacterial Viruses (Bacteriophages): These viruses infect bacteria. Example: T4 bacteriophage.

Fungal Viruses: These are viruses that infect fungi. Example: Cryphonectria parasitica (chestnut
blight).

Archaeal Viruses: These are viruses that infect archaea (primitive microorganisms). Example:
Metallosphaera turreted icosahedral virus and methanosarana spherical virus.

Detailed Examples of Virus Families

Herpesviridae

Large dsDNA viruses causing lifelong infections. Includes HSV-1, HSV-2, and Varicella-Zoster
Virus.

8.2 Orthomyxoviridae

Includes Influenza viruses. Segmented -ssRNA genome; subject to antigenic drift and shift.

8.3 Flaviviridae

+ssRNA viruses transmitted by arthropods. Includes Dengue, Zika, and Hepatitis C viruses.

8.4 Retroviridae

Enveloped viruses with reverse transcriptase. Includes HIV, HTLV-1.

9. Zoonotic and Emerging Viruses

9.1 Spillover Events

Viruses like Ebola and SARS-CoV-2 originate in animal reservoirs and cross species barriers.

9.2 Reservoir Hosts

Bats, birds, and rodents serve as natural hosts for many zoonotic viruses.

9.3 Environmental and Societal Factors

Deforestation, climate change, and global travel contribute to the emergence of new viruses.

10. Medical and Scientific Applications

10.1 Vaccines

Vaccination remains the most effective method of viral disease prevention. Examples include
mRNA vaccines for COVID-19 and live-attenuated vaccines for measles.

10.2 Gene Therapy

Viruses like adenoviruses and lentiviruses are used as vectors to deliver therapeutic genes to
target cells.

10.3 Oncolytic Viruses

Engineered viruses selectively infect and destroy cancer cells while sparing normal tissue.

11. Biotechnological Applications

11.1 CRISPR and Phages

Bacteriophages played a role in the discovery of CRISPR-Cas systems, now widely used for
genome editing.

11.2 Virus-Based Nanotechnology

Viruses are used to build nanoscale materials for drug delivery and diagnostics.

11.3 Synthetic Virology

Synthetic biology allows for the creation of designer viruses with potential uses in therapy and
research.

12. Plant and Animal Viruses

12.1 Agricultural Impact

Plant viruses like Tobacco Mosaic Virus and Banana Bunchy Top Virus cause significant crop
losses.

12.2 Veterinary Virology

Viruses such as Foot-and-Mouth Disease Virus and Avian Influenza Virus pose threats to
livestock and poultry industries.

13. Challenges in Virology

13.1 Mutation and Drug Resistance

High mutation rates in RNA viruses lead to challenges in treatment and vaccine development.

13.2 Diagnostic Limitations

Early and accurate detection of viral infections is crucial but can be hindered by low viral loads
or variability.

13.3 Biosecurity and Ethics

Research on highly pathogenic viruses raises concerns about accidental release and bioterrorism.

14. Future Perspectives in Virology

14.1 Pandemic Preparedness

Global surveillance and rapid response systems are essential for identifying and containing
outbreaks.

14.2 AI in Virus Prediction

Artificial intelligence is being used to predict viral evolution and identify potential zoonotic
threats.

14.3 Universal Vaccines

Research is underway to develop vaccines effective against all strains of a virus, such as a
universal influenza vaccine.

15. Conclusion

Viruses are fundamental to understanding biology, disease, and the evolution of life. Their study
has led to life-saving vaccines, advanced therapies, and critical biotechnological tools. Continued
research and surveillance are vital for anticipating and responding to viral threats. As science
advances, our ability to harness viruses for good while mitigating their risks will shape the future
of health and medicine.

16. References

Baltimore, D. (1971). Expression of animal virus genomes. Bacteriological Reviews, 35(3),

235–241.
ICTV. (2023). Virus Taxonomy: 2023 Release. International Committee on Taxonomy of
Viruses. https://ictv.global

Lwoff, A. (1957). The concept of virus. Journal of General Microbiology, 17(2), 239–253.

Wimmer, E. (2006). The test tube synthesis of a chemical called poliovirus. Journal of Virology,
80(6), 2711–2719.

Zhu, N., Zhang, D., Wang, W., et al. (2020). A novel coronavirus from patients with pneumonia
in China, 2019. New England Journal of Medicine, 382(8), 727–733.

Zhou, P., Yang, X. L., Wang, X. G., et al. (2020). A pneumonia outbreak associated with a new
coronavirus of probable bat origin. Nature, 579(7798), 270–273.

Fields, B. N., Knipe, D. M., & Howley, P. M. (Eds.). (2007). Fields Virology (5th ed.). Lippincott
Williams & Wilkins.

Flint, S. J., Enquist, L. W., Racaniello, V. R., & Skalka, A. M. (2015). Principles of Virology (4th
ed.). ASM Press.

Fauci, A. S., Lane, H. C., & Redfield, R. R. (2020). COVID-19—navigating the uncharted. New
England Journal of Medicine, 382(13), 1268–1269.

Woolhouse, M. E. J., & Gowtage-Sequeria, S. (2005). Host range and emerging and reemerging
pathogens. Emerging Infectious Diseases, 11(12), 1842–1847.

Weiss, R. A., & McMichael, A. J. (2004). Social and environmental risk factors in the emergence
of infectious diseases. Nature Medicine, 10(S12), S70–S76.

Drosten, C., Günther, S., Preiser, W., et al. (2003). Identification of a novel coronavirus in
patients with severe acute respiratory syndrome. New England Journal of Medicine, 348(20),
1967–1976.

Morens, D. M., & Fauci, A. S. (2008). Emerging infectious diseases: threats to human health and
global stability. PLoS Pathogens, 4(9), e1000213.

Rybicki, E. P. (1990). The classification of organisms at the edge of life, or problems with virus
systematics. South African Journal of Science, 86(5), 182–186.