MCQs for Biochemistry

Which amino acid is commonly present at the catalytic site of chymotrypsin?

a) Glutamate

b) Serine

c) Lysine

d) Alanine

Answer: b) Serine

Which enzyme deficiency is responsible for Hyperammonemia Type-I?

a) Ornithine transcarbamoylase (OTC)

b) Arginase

c) Carbamoyl Phosphate Synthetase I (CPS-I)

d) Argininosuccinate synthetase

Answer: c) Carbamoyl Phosphate Synthetase I (CPS-I)

Which organelle is responsible for ATP production through the electron transport chain?

a) Golgi body

b) Mitochondria

c) Lysosome

d) Nucleus

Answer: b) Mitochondria

Which metal ion is present in carbonic anhydrase?

a) Copper

b) Iron

c) Zinc

d) Magnesium

Answer: c) Zinc

Deficiency of Vitamin A causes which of the following conditions?

a) Scurvy

b) Night blindness

c) Beriberi
d) Pellagra

Answer: b) Night blindness

What is the total net ATP gain per glucose molecule in anaerobic glycolysis?

a) 4 ATP

b) 2 ATP

c) 6 ATP

d) 1 ATP

Answer: b) 2 ATP

Which of the following is a competitive inhibitor of xanthine oxidase?

a) Allopurinol

b) Dicoumarol

c) Methotrexate

d) Penicillin

Answer: a) Allopurinol

In non-competitive inhibition, the Vmax of the enzyme reaction is:

a) Increased

b) Decreased

c) Unchanged

d) Doubled

Answer: b) Decreased

Which of the following vitamins is involved in blood coagulation?

a) Vitamin A

b) Vitamin D

c) Vitamin K

d) Vitamin E

Answer: c) Vitamin K

Which amino acid is non-essential?

a) Leucine

b) Valine
c) Glycine

d) Histidine

Answer: c) Glycine

Which enzyme is inhibited by Lovastatin?

a) HMG-CoA reductase

b) Neuraminidase

c) Thymidylate synthase

d) Dihydrofolate reductase

Answer: a) HMG-CoA reductase

Which coenzyme is involved in the transfer of acyl groups?

a) Biotin

b) Coenzyme-A (CoA)

c) Thiamine Pyrophosphate (TPP)

d) Pyridoxal Phosphate (PLP)

Answer: b) Coenzyme-A (CoA)

Which of the following disorders is caused by a deficiency of arginase?

a) Citrullinemia

b) Argininosuccinic aciduria

c) Hyperammonemia Type-II

d) Hyperargininemia

Answer: d) Hyperargininemia

In competitive inhibition, which parameter is increased?

a) Vmax

b) Km

c) Enzyme concentration

d) Substrate concentration

Answer: b) Km

Which metabolic function is performed by the cytosol?

a) TCA cycle
b) Glycolysis

c) Electron transport chain

d) Beta-oxidation of fatty acids

Answer: b) Glycolysis

Which vitamin is an antioxidant and is essential for maintaining healthy sperm motility?

a) Vitamin A

b) Vitamin D

c) Vitamin E

d) Vitamin K

Answer: c) Vitamin E

Which enzyme requires biotin as a coenzyme for the transfer of carbon dioxide?

a) Hexokinase

b) Acetyl-CoA carboxylase

c) Phosphoglucomutase

d) Alcohol dehydrogenase

Answer: b) Acetyl-CoA carboxylase

Which of the following urea cycle disorders involves defective ornithine transport?

a) Citrullinemia

b) Hyperornithinemia

c) Hyperammonemia Type-I

d) Argininosuccinic aciduria

Answer: b) Hyperornithinemia

Which enzyme contains copper and is responsible for melanin synthesis?

a) Alcohol dehydrogenase

b) Hexokinase

c) Tyrosinase

d) Xanthine oxidase

Answer: c) Tyrosinase

Which coenzyme transfers phosphate groups during biochemical reactions?

b) Tetrahydrofolate (FH4)

c) Coenzyme-A (CoA)

d) Adenosine Triphosphate (ATP)

Answer: d) Adenosine Triphosphate (ATP)

Which of the following is the key function of the lysosome?

a) Electron transport

b) Protein maturation

c) Degradation of cellular waste

d) Protein synthesis

Answer: c) Degradation of cellular waste

Which competitive inhibitor targets thymidylate synthase?

a) 5-Fluorouracil

b) Allopurinol

c) Methotrexate

d) Lovastatin

Answer: a) 5-Fluorouracil

What is the biochemical significance of the mitochondrial electron transport chain?

a) Glycolysis

b) ATP generation

c) Fatty acid synthesis

d) Urea synthesis

Answer: b) ATP generation

Which amino acid is classified as essential?

a) Glycine

b) Alanine

c) Leucine

d) Glutamate

Answer: c) Leucine
In competitive inhibition, which factor can relieve inhibition?

a) Increase in enzyme concentration

b) Increase in substrate concentration

c) Decrease in Km

d) Increase in product concentration

Answer: b) Increase in substrate concentration

Which enzyme is involved in cholesterol synthesis and is inhibited by statins?

a) HMG-CoA reductase

b) Pyruvate kinase

c) Carbonic anhydrase

d) Xanthine oxidase

Answer: a) HMG-CoA reductase

What is the main product of heme degradation in the liver?

a) Biliverdin

b) Urobilin

c) Bilirubin

d) Stercobilin

Answer: c) Bilirubin

Which organelle participates in urea synthesis and gluconeogenesis?

a) Cytosol

b) Lysosome

c) Golgi body

d) Mitochondria

Answer: d) Mitochondria

Which of the following disorders is inherited in an autosomal recessive manner and involves high levels
of citrulline?

a) Hyperammonemia Type-I

b) Hyperammonemia Type-II

c) Citrullinemia
d) Hyperargininemia

Answer: c) Citrullinemia

Which vitamin deficiency can result in bleeding disorders and impaired clotting?

a) Vitamin A

b) Vitamin D

c) Vitamin E

d) Vitamin K

Answer: d) Vitamin K

Which amino acid is present at the active site of carbonic anhydrase?

a) Serine

b) Histidine

c) Aspartate

d) Lysine

Answer: b) Histidine

Which of the following vitamins regulates calcium levels in the body?

a) Vitamin A

b) Vitamin D

c) Vitamin E

d) Vitamin K

Answer: b) Vitamin D

Which enzyme is responsible for the final step in glycolysis?

a) Hexokinase

b) Pyruvate kinase

c) Phosphofructokinase

d) Glucose-6-phosphatase

Answer: b) Pyruvate kinase

In urea cycle disorders, increased levels of ammonia and citrulline are characteristic of:

a) Argininosuccinic aciduria

b) Hyperornithinemia
c) Hyperammonemia Type-I

d) Citrullinemia

Answer: d) Citrullinemia

Which enzyme contains magnesium as a cofactor?

a) Hexokinase

b) Tyrosinase

c) Xanthine oxidase

d) Lecithinase

Answer: a) Hexokinase

Which coenzyme is essential for the transfer of amino groups?

a) Pyridoxal Phosphate (PLP)

b) Thiamine Pyrophosphate (TPP)

c) Biotin

d) Coenzyme-A (CoA)

Answer: a) Pyridoxal Phosphate (PLP)

What is the primary function of the Golgi apparatus?

a) ATP generation

b) Protein maturation

c) Degradation of lipids

d) DNA replication

Answer: b) Protein maturation

Which type of inhibition is generally irreversible?

a) Competitive inhibition

b) Non-competitive inhibition

c) Feedback inhibition

d) Uncompetitive inhibition

Answer: b) Non-competitive inhibition

Which urea cycle disorder involves the accumulation of argininosuccinate in blood and urine?

a) Hyperammonemia Type-I
b) Argininosuccinic aciduria

c) Citrullinemia

d) Hyperornithinemia

Answer: b) Argininosuccinic aciduria

Which vitamin deficiency is associated with rickets in children?

a) Vitamin A

b) Vitamin D

c) Vitamin E

d) Vitamin K

Answer: b) Vitamin D

Which enzyme inhibitor is used to block folate synthesis in bacteria?

a) Sulfonamides

b) Allopurinol

c) Dicoumarol

d) Lovastatin

Answer: a) Sulfonamides

Which of the following enzymes is inhibited by methotrexate?

a) Xanthine oxidase

b) Dihydrofolate reductase

c) Thymidylate synthase

d) Neuraminidase

Answer: b) Dihydrofolate reductase

Which organelle is involved in both beta-oxidation of fatty acids and ketone body production?

a) Mitochondria

b) Lysosome

c) Golgi body

d) Cytosol

Answer: a) Mitochondria

Which of the following is a fat-soluble vitamin?

b) Vitamin B12

c) Vitamin A

d) Vitamin B6

Answer: c) Vitamin A

Which enzyme is inhibited by dicoumarol, interfering with blood clotting?

a) Neuraminidase

b) Vitamin K epoxide reductase

c) HMG-CoA reductase

d) Xanthine oxidase

Answer: b) Vitamin K epoxide reductase

Which amino acid is a precursor for serotonin synthesis?

a) Tyrosine

b) Tryptophan

c) Phenylalanine

d) Arginine

Answer: b) Tryptophan

What is the primary function of pyrimidine synthesis in cells?

a) ATP production

b) DNA and RNA synthesis

c) Protein degradation

d) Lipid metabolism

Answer: b) DNA and RNA synthesis

Which vitamin deficiency causes night blindness?

a) Vitamin A

b) Vitamin D

c) Vitamin E

d) Vitamin K

Answer: a) Vitamin A
Which coenzyme transfers one-carbon groups in metabolic reactions?

a) Coenzyme-A (CoA)

b) Biotin

c) Tetrahydrofolate (FH4)

d) Pyridoxal Phosphate (PLP)

Answer: c) Tetrahydrofolate (FH4)

Which enzyme is involved in heme degradation to form biliverdin?

a) Catalase

b) Cytochrome oxidase

c) Heme oxygenase

d) Peroxidase

Answer: c) Heme oxygenase