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ABO Rh PROCEDURE

This document outlines the procedural method for determining ABO and Rh blood groups in a laboratory setting. It details the necessary specimens, reagents, supplies, and a step-by-step procedure for conducting the tests. The procedure includes labeling test tubes, adding specific drops of reagents and patient samples, and interpreting the results for agglutination.

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Raki Dallas
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6 views1 page

ABO Rh PROCEDURE

This document outlines the procedural method for determining ABO and Rh blood groups in a laboratory setting. It details the necessary specimens, reagents, supplies, and a step-by-step procedure for conducting the tests. The procedure includes labeling test tubes, adding specific drops of reagents and patient samples, and interpreting the results for agglutination.

Uploaded by

Raki Dallas
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOCX, PDF, TXT or read online on Scribd
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LABORATORY AND PATHOLOGY DEPARTMENT

BLOOD BANK SECTION

PROCEDURAL METHOD ON ABO AND Rh GROUP DETERMINATION

A. SPECIMEN
- Anti-coagulated Whole blood (Lavender Top)
- Serum

B. REAGENTS
- Anti-sera A, Anti-sera B, Anti-sera D
- Red Cell Suspension – A1 and B red cell. (Commercially prepared or laboratory
prepared from a 3% to 5% red cells suspension of pooled from known blood type “A”
and known blood type “B” donor red cell)

C. SUPPLIES
- Test tubes
- Calibrated plastic pipette (0.5 – 5 uL)
- Buffered Normal Saline Solution (NSS)
- Serologic Centrifuge

D. PROCEDURE

1. Label five (5) clean test tubes respectively as: Anti-A, Anti-B and Anti-D for forward grouping
and Known “A” and Known “B” (A1 cells & B cells) for reverse grouping.
2. Place 1 drop each of Anti-A, Anti-B and Anti-D in their respective labeled clean test tube.
3. Place 1 drop (approx.. 50 uL) each of Known “A” (A 1 cells) and Known “B” (B cells) in their
respective labeled clean test tube.
4. Add 1 drop of patient’s red cell suspension (3% - 5%) to test tube labeled Anti-A, Anti-B and
Anti-D.
5. Add 2 drops of patient’s serum / plasma to test tubes labeled Known ”A” and Known “B”.
6. Mix gently the content of all tubes and centrifuge them for the calibrated spin time.
7. Gently re-suspend cell buttons and examine them for agglutination.
8. Read, interpret, and record the test results accordingly.

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