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4-Functions-and-Procedures-Perfromed-by-a-Laboratory-Assistant-Aide-Assigned-to-the-Testing-Areas

The document outlines the functions and procedures of a laboratory assistant/aide in testing areas, including washing and sterilizing glassware, cleaning work areas, and managing laboratory waste. It details the proper methods for cleaning and sterilizing laboratory instruments, as well as the correct handling of accumulated waste materials. Additionally, it emphasizes the importance of maintaining a safe and organized laboratory environment.

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allysa alejo
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5 views

4-Functions-and-Procedures-Perfromed-by-a-Laboratory-Assistant-Aide-Assigned-to-the-Testing-Areas

The document outlines the functions and procedures of a laboratory assistant/aide in testing areas, including washing and sterilizing glassware, cleaning work areas, and managing laboratory waste. It details the proper methods for cleaning and sterilizing laboratory instruments, as well as the correct handling of accumulated waste materials. Additionally, it emphasizes the importance of maintaining a safe and organized laboratory environment.

Uploaded by

allysa alejo
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as TXT, PDF, TXT or read online on Scribd
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THE FUNCTIONS AND PROCEDURES

PERFORMED BY A LABORATORY
ASSISTANT/AIDE ASSIGNED TO THE
TESTING AREAS
LEARNING OUTCOMES
STATE THE FUNCTIONS OF A LABORATORY ASSISTANT/AIDE ASSIGNED TO THE
1 LABORATORY TESTING AREAS

DISCUSS THE CORRECT PROCEDURE IN WASHING LABORATORY


2 GLASSWARE/INSTRUMENTS AFTER USE

EXPLAIN THE PROPER WAY OF STERILIZING AND STORING CLEAN


3 GLASSWARE/INSTRUMENTS

ILLUSTRATE THE PROPER CLEANING AND MAINTEANCE PROCEDURE OF


4 LABORATORY WORK AREAS

5 ENUMERATE THE COMPONENTS OF THE LABORATORY WASTE MANAGEMENT PLAN

DISCUSS THE PROPER HANDLING OF LABORATORY-ACCUMULATED WASTE


6 MATERIALS
THE FUNCTIONS OF A LABORATORY ASSISTANT/AIDE
ASSIGNED TO THE TESTING AREAS

• Washing and sterilizing of laboratory glassware


and instruments
• Cleaning laboratory workbenches
• Helping mixing chemical solutions
• Preparing and segregating laboratory-accumulated
waste materials for disposal
• Cleaning and restocking the laboratory supplies
WHOA!
Washing Laboratory Glassware

CLEAN STERILE GREASE-FREE

DRIED IN A DRYING OVEN AT 150C, CAPPED USING AN ALUMINUM FOIL


REMOVING GREASE

1. Boil the glassware in a weak solution of sodium


carbonate, acetone, or any fat solvent
2. For silicone grease, soak the stopcock plug or barrel in a
warm decahydronaphthalene for two (2) hours
3. Drain, rinse with acetone or use fuming sulfuric acid for 30
minutes
4. Rinse off all of the cleaning agents
RINSING
1. GLASSWARE – rinse the glassware using running tap water. Rinse
with distilled water or use a large bath of distilled water
2. TEST TUBES, GRADUATED CYLINDERS AN FLASKS – allow the tap
water to run into and over them. Then, partly fill each pace with
water, shake, and empty for about six times
3. PIPETS - attach a piece of rubbing tubing to the faucet, then connect
the delivery end to a hose to allow water to run through them
CULTURE TUBES
1. Autoclave for 30 mins at 121°C
2. If there are media that solidify upon cooling, pour them out while the
tubes are still hot.
3. Empty the tubes
4. Brush with detergent and water
5. Rinse with tap water thoroughly
6. Rinse using distilled water
7. Place the tubes in the basket and let them dry
DISHES AND CULTURE BOTTLES
1. Sterilize and clean following the procedure for cleaning culture
tubes
2. Wrap them in a heavy aper or place them in a petri dish can
3. Sterilize the dishes and culture bottles using autoclave or a dry-air
sterilizer
PIPETS
1. Place pipets with tips down in a cylinder or tall jar of water
immediately after use
2. Do not drop them into the jar because the tips may break
3. Place a pad of cotton or glass wool at the bottom of the jar to avoid
breaking the tips
4. Make sure that the water level is high enough to immerse most if not
the whole pipet
5. Drain and place in a cylinder or jar of dissolved detergent. Soak in a
jar of chromic acid cleaning solution if exceptionally dirty
PIPETS
6. After soaking, drain them. Run tap water over and through the pipets
until you are sure that all traces of dirt are removed.
7. Soak the pipets in distilled water for at least one hour.
8. Remove them from distilled water.
9. Rinse and dry the outside portion using a cloth.
10.Shake the water out of the pipets and let them dry
STERILIZING AND STORING CLEAN GLASSWARE

• Glassware could be placed in a large bucket or boiler


• Add 1-2% soap or detergent before letting it boil for 30 minutes
• Rinse with tap water, scrubbed with detergent and then rinse
• An autoclave, large steam oven, or a similar equipment can be used
to sterilize the glassware
STERILIZING AND STORING CLEAN GLASSWARE

• Test tubes, culture tubes, flasks and other laboratory wares are dried
by hanging them on wooden pegs
• They could be air dried by placing them in baskets with mouths
downward or simply dried using an oven (140°C)
• To ensure that the vessel mouth is clean, the drying basket should be
lined with a clean cloth
• Pipets and cylinders are dried by standing them on a folded towel
• Clean glassware pieces are protected from dust by plugging them
with cotton, corking or placing a heavy piece of paper over the mouth
Additional precautions to ensure pieces of glassware are clean but also free from
damage and contaiminants

1. Always examine the glassware for damage and discard any cracked
or broken glassware in the proper disposal container
2. Clean and remove any residue including all tapes and labels
3. Use automatic cleaning apparatuses like lab washing machine,
automated pipette, burette washers, if available
4. Autoclave contaminated glassware to sterilize
5. Air-dry the glassware if it will not be used immediately. Hot air
drying racks can be used to accelerate the drying process
6. Store the glassware in the proper place after drying
Cleaning and Maintenace Procedure of the Laboratory Work Areas

1. Floors should be clutter-free.


2. Materials should be returned to their designated places after use
3. The laboratory exit floor plan should have two clear passageways
4. Safety showers, eyewashes, fire extinguishers, and electrical control
boxes should be accessible and strategically placed
5. Sink traps and floor drains should be maintained and filled with
water to avoid sewer gases from escaping the laboratories
6. Bench apparatus should be placed away from any edges
7. The cleaning of the laboratory working area should be scheduled
upon completion of tests or at the end of the working day
Cleaning and Maintenace Procedure of the Laboratory Work Areas

8. Bench tops and liners are kept free from visible contamination
9. Spills should be cleaned immediately to prevent slips, trips, and falls
10. Doors and drawers are kept closed and passageways should be free
from obstructions
CLEANING MATERIALS

• 12% Lysol
• 3.5% Sodium hypochlorite/Household bleach
• Spray bottles
• PPE
• Broom
• Mop
• Bucket
CLEANING REAGENTS

• 1% Sodium hypochlorite solution – for daily cleaning


Add 10 mL of sodium hypochlorite solution to 1000mL of water

• 70% Ethanol
To produce 1 liter, take 700 mL of absolute ethanol and add 300mL of
distilled water

• Lysol (5% phenolic solution)


1 liter of 5% Lysol is produced by adding 450 mL 12% Lysol to 550 mL of
distilled water
WEEKLY CLEANING of the WORK AREA

• Using soap solution followed by 5% Lysol solution to clean outer


surfaces of the cupboards, laboratory trolleys, sinks and chairs
• Cleaning the ultrasonic water bath using hypochlorite (bleach)
solution and refilling with fresh distilled water
• Cleaning the racks and tubes on a weekly basis by immersing them in
5% Lysol and allowing the, to air-dry
• The racks for the molecular testing are cleaned after use by
immersing them in 1% sodium hypochlorite (bleach) solution
Proper Handling of Laboratory-accumulated Waste Materials

• Collect hazardous or flammable waste solvents in containers pending


transfer or disposal
• Do not mix incompatible waste to avoid occurrence of heat
generation, gas evolution, or other reactions
• Always segregate wastes according to how they will be managed
• Always observe the compatibility of containers and the waste
contents. Always keep containers closed
• Provide a container (plastic/metal) for collection of liquid waste and
occasionally clean the frame arresters, which can be plugged if there
are sediments
Proper Handling of Laboratory-accumulated Waste Materials

• Do not use galvanized steel safety can for halogenated waste solvents
to avoid corrosion and leaks
• Label the waste containers with their contents clearly and properly
• Cap the waste containers after use.
• Separately collect aqueous wastes from organic solvent wastes.
Collect them in a container that is resistant to corrosion.
• Place solid chemical wastes in a labeled container prior to disposal
• Dispose non-hazardous solid wastes using the laboratory trash or
segregate them for recycling.
• When disposing empty containers, remove the labels and empty the
contents. Rinse the container if necessary.
PHILIPPINE HEALTH
CARE WASTE
MANAGEMENT
PROGRAM

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